Monneaux F, Muller S
Laboratory protocols for the identification of Th cell epitopes on self-antigens in mice with systemic autoimmune diseases Article de journal
Dans: Journal of Immunological Methods, vol. 244, no. 1-2, p. 195–204, 2000, ISSN: 0022-1759.
Résumé | Liens | BibTeX | Étiquettes: Animals, Antigen Presentation, Antigen-Presenting Cells, Autoantigens, B-Lymphocytes, Coculture Techniques, Epitopes, Female, Flow Cytometry, I2CT, Inbred MRL lpr, Inbred NZB, Lupus Erythematosus, Lymphocyte Activation, Mice, Monneaux, Ribonucleoproteins, Small Nuclear, Systemic, T-Lymphocyte, Team-Dumortier, Th1 Cells, Th2 Cells
@article{monneaux_laboratory_2000,
title = {Laboratory protocols for the identification of Th cell epitopes on self-antigens in mice with systemic autoimmune diseases},
author = {F Monneaux and S Muller},
doi = {10.1016/s0022-1759(00)00256-8},
issn = {0022-1759},
year = {2000},
date = {2000-10-01},
journal = {Journal of Immunological Methods},
volume = {244},
number = {1-2},
pages = {195--204},
abstract = {T cells play a critical role in both the immunological and clinical manifestations of systemic autoimmune diseases such as systemic lupus erythematosus (SLE). Although in normal mice multiple T cell epitopes have been characterized in several self-proteins, there is little information on the fine specificity of autoreactive T cells in lupus model mice and humans. In SLE-prone mice and humans, the only Th cell epitopes identified at the molecular level in self-antigens concern histones and nucleosomes, and the 70-kD U1-snRNP protein. T cell characterization in certain autoimmune mice such as MRL lpr/lpr and NZB/NZW mice has been largely impaired by their hyporesponsiveness in response to mitogen and minimal IL-2 secretion. In addition, MRL lpr/lpr mice also develop lymphadenopathy characterized by the progressive accumulation of functionally immature CD4(-) CD8(-) T cells. It is therefore important to optimize the methods used to measure T cell proliferation and cytokine production ex vivo in order to identify minimal activation in the presence of appropriate antigen. The protocol described in this article has been used for identifying in young MRL lpr/lpr and NZB/NZW mice a CD4(+) T cell epitope in the murine 70-kD U1-RNP protein.},
keywords = {Animals, Antigen Presentation, Antigen-Presenting Cells, Autoantigens, B-Lymphocytes, Coculture Techniques, Epitopes, Female, Flow Cytometry, I2CT, Inbred MRL lpr, Inbred NZB, Lupus Erythematosus, Lymphocyte Activation, Mice, Monneaux, Ribonucleoproteins, Small Nuclear, Systemic, T-Lymphocyte, Team-Dumortier, Th1 Cells, Th2 Cells},
pubstate = {published},
tppubtype = {article}
}
Dumortier H, Monneaux F, Jahn-Schmid B, Briand J P, Skriner K, Cohen P L, Smolen J S, Steiner G, Muller S
B and Ŧ cell responses to the spliceosomal heterogeneous nuclear ribonucleoproteins A2 and B1 in normal and lupus mice Article de journal
Dans: Journal of Immunology (Baltimore, Md.: 1950), vol. 165, no. 4, p. 2297–2305, 2000, ISSN: 0022-1767.
Résumé | Liens | BibTeX | Étiquettes: Amino Acid Sequence, Animals, Autoantibodies, B-Lymphocytes, Dumortier, Epitope Mapping, Female, Heterogeneous Nuclear, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Heterogeneous-Nuclear Ribonucleoproteins, Humans, I2CT, Immunoglobulin G, Inbred BALB C, Inbred C57BL, Inbred CBA, Inbred MRL lpr, Injections, Lupus Nephritis, Lymphocyte Activation, Male, Mice, Molecular Sequence Data, Monneaux, Peptide Fragments, Recombinant Proteins, Ribonucleoproteins, RNA, Spliceosomes, Subcutaneous, T-Lymphocytes, Team-Dumortier, transgenic
@article{dumortier_b_2000,
title = {B and Ŧ cell responses to the spliceosomal heterogeneous nuclear ribonucleoproteins A2 and B1 in normal and lupus mice},
author = {H Dumortier and F Monneaux and B Jahn-Schmid and J P Briand and K Skriner and P L Cohen and J S Smolen and G Steiner and S Muller},
doi = {10.4049/jimmunol.165.4.2297},
issn = {0022-1767},
year = {2000},
date = {2000-08-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {165},
number = {4},
pages = {2297--2305},
abstract = {Autoantibodies directed against spliceosomal heterogeneous nuclear ribonucleoproteins (hnRNPs) are a typical feature of rheumatoid arthritis, systemic lupus erythematosus, and mixed-connective tissue disease. With the aim of investigating a potential pathogenic role of these Abs, we have studied the Ab response to A2/B1 hnRNPs in different murine models of lupus. The specificity of anti-A2/B1 Abs was tested with a series of 14 overlapping synthetic peptides covering the region 1-206 of A2 that contains most of the epitopes recognized by patients' Abs. A major epitope recognized very early during the course of the disease by Abs from most of MRL lpr/lpr mice but not from other lupus mice and from mice of different MHC haplotypes immunized against B1 was identified in residues 50-70. This peptide contains a highly conserved sequence RGFGFVTF also present in other hnRNPs and small nuclear ribonucleoproteins. Abs reacting with a second A2 epitope identified in residues 35-55 were detectable several weeks later, suggesting an intramolecular B cell epitope spreading during the course of the disease. We identified several T cell epitopes within the region 35-175 that generated an effective Th cell response with IL-2 and IFN-gamma secretion in nonautoimmune CBA/J mice sharing the same MHC haplotype H-2k as MRL/lpr mice. None of the peptides stimulated T cells primed in vivo with B1. Because Abs to peptide 50-70 were detected significantly earlier than Abs reacting with other A2 peptides and the protein itself, it is possible that within the protein, this segment contains residues playing an initiator role in the induction of the anti-A2/B1 and antispliceosome Ab response.},
keywords = {Amino Acid Sequence, Animals, Autoantibodies, B-Lymphocytes, Dumortier, Epitope Mapping, Female, Heterogeneous Nuclear, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Heterogeneous-Nuclear Ribonucleoproteins, Humans, I2CT, Immunoglobulin G, Inbred BALB C, Inbred C57BL, Inbred CBA, Inbred MRL lpr, Injections, Lupus Nephritis, Lymphocyte Activation, Male, Mice, Molecular Sequence Data, Monneaux, Peptide Fragments, Recombinant Proteins, Ribonucleoproteins, RNA, Spliceosomes, Subcutaneous, T-Lymphocytes, Team-Dumortier, transgenic},
pubstate = {published},
tppubtype = {article}
}
Monneaux F, Briand J P, Muller S
Dans: European Journal of Immunology, vol. 30, no. 8, p. 2191–2200, 2000, ISSN: 0014-2980.
Résumé | Liens | BibTeX | Étiquettes: Amino Acid Motifs, Animals, Antigen-Presenting Cells, Autoimmunity, B-Lymphocytes, CD4-Positive T-Lymphocytes, Epitopes, Female, I2CT, Inbred BALB C, Inbred CBA, Inbred MRL lpr, Lupus Vulgaris, Lymphocyte Activation, Mice, Monneaux, Peptide Fragments, Ribonucleoprotein, T-Lymphocyte, Team-Dumortier, U1 Small Nuclear
@article{monneaux_b_2000,
title = {B and Ŧ cell immune response to small nuclear ribonucleoprotein particles in lupus mice: autoreactive CD4(+) Ŧ cells recognize a Ŧ cell epitope located within the RNP80 motif of the 70K protein},
author = {F Monneaux and J P Briand and S Muller},
doi = {10.1002/1521-4141(2000)30:8<2191::AID-IMMU2191>3.0.CO;2-R},
issn = {0014-2980},
year = {2000},
date = {2000-08-01},
journal = {European Journal of Immunology},
volume = {30},
number = {8},
pages = {2191--2200},
abstract = {Systemic lupus erythematosus is characterized by the presence of high titers of autoantibodies reacting with various components of the U1 small nuclear ribonucleoprotein particle (snRNP). It has been suggested that these antibodies are produced by an antigen-driven mechanism under the dependence of antigen-specific T cells. To investigate the role of T cell help in this process, we sought, with 20 overlapping peptides, the Th epitopes on the U1-70K snRNP in unprimed H-2(k) MRL / lpr lupus mice and immunized CBA normal mice. The peptide 131 - 151 was recognized by both IgG autoantibodies and CD4(+) T cells from 7 - 9-week-old MRL / lpr mice. In this test, antigen-presenting cells (APC) from MRL / lpr mice were required; APC from naive CBA mice failed to stimulate CD4(+) cells from MRL / lpr mice. The potential role of MRL / lpr B cells as APC, the expression of MHC class II molecules at their surface and their activation state (expression of CD69, CD80 / B7-1 and CD86 / B7-2 molecules) were studied. Peptide 131 - 151 bound both I-A(k) and I-E(k) class II molecules and favored an IL-2-positive T cell response but not IFN-gamma, IL-6 and IL-10 secretion. Segment 131 - 151 is localized within the RNP80 motif and contains residues that are highly conserved in many nuclear, nucleolar and cytoplasmic RNA binding proteins.},
keywords = {Amino Acid Motifs, Animals, Antigen-Presenting Cells, Autoimmunity, B-Lymphocytes, CD4-Positive T-Lymphocytes, Epitopes, Female, I2CT, Inbred BALB C, Inbred CBA, Inbred MRL lpr, Lupus Vulgaris, Lymphocyte Activation, Mice, Monneaux, Peptide Fragments, Ribonucleoprotein, T-Lymphocyte, Team-Dumortier, U1 Small Nuclear},
pubstate = {published},
tppubtype = {article}
}
Obrecht-Pflumio S., Dirheimer G.
In vitro DNA and dGMP adducts formation caused by ochratoxin A Article de journal
Dans: Chem Biol Interact, vol. 127, no. 1, p. 29-44, 2000, (0009-2797 Journal Article).
Résumé | BibTeX | Étiquettes: Acid/metabolism, Adducts/*metabolism, Agents/pharmacology, Animals, Arachidonic, Carcinogens/pharmacology, Chelating, Chromatography, Deferoxamine/pharmacology, Deoxyguanine, DNA, Female, Kidney/ultrastructure, Liver/metabolism, Male, Mice, Microsomes, Microsomes/metabolism, Mycotoxins/pharmacology, NADP/metabolism, Nucleotides/*metabolism, Nucleotides/metabolism, Ochratoxins/*pharmacology, Peroxidase/metabolism, Rabbits, Spectrophotometry
@article{,
title = {In vitro DNA and dGMP adducts formation caused by ochratoxin A},
author = { S. Obrecht-Pflumio and G. Dirheimer},
year = {2000},
date = {2000-01-01},
journal = {Chem Biol Interact},
volume = {127},
number = {1},
pages = {29-44},
abstract = {Ochratoxin A (OTA), a nephrotoxic and nephrocarcinogenic mycotoxin, leads to the formation of DNA adducts after administration to animals. This could be due to an epigenetic effect. In vitro assays can exclude an indirect effect, where the xenobiotic can generate, in vivo, endogenous reactive compounds which give adducts on DNA. Microsomes prepared from mice or rabbit kidney and liver, used as metabolic activators, were incubated in the presence of commercial salmon testes DNA and OTA, with NADPH or arachidonic acid used as cofactors. Upto 126 DNA adducts for 10(9) nucleotides were detected using the 32P postlabeling method after incubation with the mouse kidney system. Similar results were obtained with rabbit kidney microsomes. Using liver microsomes, the number of DNA adducts detected was much lower. When NADPH was used as a cosubstrate (to explore the cytochrome P450 metabolic pathways), with mice kidney microsomes, the adduct level was only 44% of the one obtained with arachidonic acid. These results lend support to the hypothesis of the preferential activation of OTA by the peroxidase activity of prostaglandin synthases and/or lipoxygenases to direct genotoxic metabolites, and are in agreement with the previously obtained results after in vivo treatment of mice. In order to identify the nucleotides of DNA modified by the OTA metabolites, dAMP, dGMP, dTMP and dCMP were used as substrates under the same conditions as with DNA. The adducts were found only on dGMP. The total adduct level was of 344 adducts per 10(9) nucleotides with the appearance of three major adducts in the presence of arachidonic acid. With NADPH, 271 adducts were obtained per 10(9) nucleotides, with again three major adducts, but only two of them were similar to two adducts obtained in the presence of arachidonic acid. Desferal (desferrioxamine B methanesulphonate), at a 50 microM concentration, did not reduce the adduct level. Adducts were also obtained when polydG, polydC and dG-p-dG were used as alternative substrates, whereas no adducts were obtained with polydA, polydT and polydC. The major adduct obtained after incubation of DNA with OTA, comigrated with the major adduct obtained with dGMP, in two chromatographic solvents. These results show that OTA is metabolized to genotoxic metabolite(s) which interact with the guanine residues of DNA.},
note = {0009-2797
Journal Article},
keywords = {Acid/metabolism, Adducts/*metabolism, Agents/pharmacology, Animals, Arachidonic, Carcinogens/pharmacology, Chelating, Chromatography, Deferoxamine/pharmacology, Deoxyguanine, DNA, Female, Kidney/ultrastructure, Liver/metabolism, Male, Mice, Microsomes, Microsomes/metabolism, Mycotoxins/pharmacology, NADP/metabolism, Nucleotides/*metabolism, Nucleotides/metabolism, Ochratoxins/*pharmacology, Peroxidase/metabolism, Rabbits, Spectrophotometry},
pubstate = {published},
tppubtype = {article}
}
Postawski K., Olech-Fudali E., Jakowicki J. A., Korobowicz E., Keith G., Baranowski W.
[Overall genomic DNA methylation in relation to estrogen] Article de journal
Dans: Ginekol Pol, vol. 71, no. 9, p. 1206-11, 2000, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: 80, Abstract, Aged, and, Biopsy, DNA, English, Estrogen/*metabolism, Female, Gov't, Human, Methylases/metabolism, Methylation, Middle, modification, Neoplasms/*metabolism/*pathology, Non-U.S., over, Progesterone/*metabolism, Receptors, Support, Uterine, Uterus/*metabolism/pathology
@article{,
title = {[Overall genomic DNA methylation in relation to estrogen]},
author = { K. Postawski and E. Olech-Fudali and J. A. Jakowicki and E. Korobowicz and G. Keith and W. Baranowski},
year = {2000},
date = {2000-01-01},
journal = {Ginekol Pol},
volume = {71},
number = {9},
pages = {1206-11},
abstract = {Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors. Additionally, there was no low DNA methylation in ER negative uterine cancer tissues. Decrease of the overall genomic DNA methylation could be related to the increase of ER/PR ratio, however it was not significant in our investigation. The potential role of steroid receptors status in uterine cancer tissue is discussed.},
note = {0017-0011
Journal Article},
keywords = {80, Abstract, Aged, and, Biopsy, DNA, English, Estrogen/*metabolism, Female, Gov't, Human, Methylases/metabolism, Methylation, Middle, modification, Neoplasms/*metabolism/*pathology, Non-U.S., over, Progesterone/*metabolism, Receptors, Support, Uterine, Uterus/*metabolism/pathology},
pubstate = {published},
tppubtype = {article}
}
Manfruelli P, Reichhart Jean-Marc, Steward R, Hoffmann Jules A, Lemaitre Bruno
A mosaic analysis in Drosophila fat body cells of the control of antimicrobial peptide genes by the Rel proteins Dorsal and DIF Article de journal
Dans: EMBO J., vol. 18, no. 12, p. 3380–3391, 1999, ISSN: 0261-4189.
Résumé | Liens | BibTeX | Étiquettes: Animals, Anti-Infective Agents, Cell Surface, Clone Cells, DNA-Binding Proteins, Fat Body, Female, Gene Expression Regulation, Genes, hoffmann, Insect, Insect Proteins, Larva, M3i, Male, Membrane Glycoproteins, Mosaicism, Mutation, Nuclear Proteins, Phosphoproteins, Receptors, reichhart, Reporter, Signal Transduction, Toll-Like Receptors, Transcription Factors
@article{manfruelli_mosaic_1999,
title = {A mosaic analysis in Drosophila fat body cells of the control of antimicrobial peptide genes by the Rel proteins Dorsal and DIF},
author = {P Manfruelli and Jean-Marc Reichhart and R Steward and Jules A Hoffmann and Bruno Lemaitre},
doi = {10.1093/emboj/18.12.3380},
issn = {0261-4189},
year = {1999},
date = {1999-06-01},
journal = {EMBO J.},
volume = {18},
number = {12},
pages = {3380--3391},
abstract = {Expression of the gene encoding the antifungal peptide Drosomycin in Drosophila adults is controlled by the Toll signaling pathway. The Rel proteins Dorsal and DIF (Dorsal-related immunity factor) are possible candidates for the transactivating protein in the Toll pathway that directly regulates the drosomycin gene. We have examined the requirement of Dorsal and DIF for drosomycin expression in larval fat body cells, the predominant immune-responsive tissue, using the yeast site-specific flp/FRT recombination system to generate cell clones homozygous for a deficiency uncovering both the dorsal and the dif genes. Here we show that in the absence of both genes, the immune-inducibility of drosomycin is lost but can be rescued by overexpression of either dorsal or dif under the control of a heat-shock promoter. This result suggests a functional redundancy between both Rel proteins in the control of drosomycin gene expression in the larvae of Drosophila. Interestingly, the gene encoding the antibacterial peptide Diptericin remains fully inducible in the absence of the dorsal and dif genes. Finally, we have used fat body cell clones homozygous for various mutations to show that a linear activation cascade Spaetzle--textgreater Toll--textgreaterCactus--textgreaterDorsal/DIF leads to the induction of the drosomycin gene in larval fat body cells.},
keywords = {Animals, Anti-Infective Agents, Cell Surface, Clone Cells, DNA-Binding Proteins, Fat Body, Female, Gene Expression Regulation, Genes, hoffmann, Insect, Insect Proteins, Larva, M3i, Male, Membrane Glycoproteins, Mosaicism, Mutation, Nuclear Proteins, Phosphoproteins, Receptors, reichhart, Reporter, Signal Transduction, Toll-Like Receptors, Transcription Factors},
pubstate = {published},
tppubtype = {article}
}
Lowenberger C A, Kamal S, Chiles J, Paskewitz S, Bulet Philippe, Hoffmann Jules A, Christensen B M
Mosquito-Plasmodium interactions in response to immune activation of the vector Article de journal
Dans: Exp. Parasitol., vol. 91, no. 1, p. 59–69, 1999, ISSN: 0014-4894.
Résumé | Liens | BibTeX | Étiquettes: Aedes, Animals, Anopheles, Culicidae, Defensins, Digestive System, Escherichia coli, Female, Genetic, Hemolymph, hoffmann, Insect Vectors, M3i, messenger, Micrococcus luteus, Plasmodium, Plasmodium berghei, Plasmodium gallinaceum, Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Transcription
@article{lowenberger_mosquito-plasmodium_1999,
title = {Mosquito-Plasmodium interactions in response to immune activation of the vector},
author = {C A Lowenberger and S Kamal and J Chiles and S Paskewitz and Philippe Bulet and Jules A Hoffmann and B M Christensen},
doi = {10.1006/expr.1999.4350},
issn = {0014-4894},
year = {1999},
date = {1999-01-01},
journal = {Exp. Parasitol.},
volume = {91},
number = {1},
pages = {59--69},
abstract = {During the development of Plasmodium sp. within the mosquito midgut, the parasite undergoes a series of developmental changes. The elongated ookinete migrates through the layers of the midgut where it forms the oocyst under the basal lamina. We demonstrate here that if Aedes aegypti or Anopheles gambiae, normally susceptible to Plasmodium gallinaceum and P. berghei, respectively, are immune activated by the injection of bacteria into the hemocoel, and subsequently are fed on an infectious bloodmeal, there is a significant reduction in the prevalence and mean intensity of infection of oocysts on the midgut. Only those mosquitoes immune activated prior to, or immediately after, parasite ingestion exhibit this reduction in parasite development. Mosquitoes immune activated 2-5 days after bloodfeeding show no differences in parasite burdens compared with naive controls. Northern analyses reveal that transcriptional activity for mosquito defensins is not detected in the whole bodies of Ae. aegypti from 4 h to 10 days after ingesting P. gallinaceum, suggesting that parasite ingestion, passage from the food bolus through the midgut, oocyst formation, and subsequent release of sporozoites into the hemolymph do not induce the production of defensin. However, reverse transcriptase-PCR of RNA isolated solely from the midguts of Ae. aegypti indicates that transcription of mosquito defensins occurs in the midguts of naive mosquitoes and those ingesting an infectious or noninfectious bloodmeal. Bacteria-challenged Ae. aegypti showed high levels of mature defensin in the hemolymph that correlate with a lower prevalence and mean intensity of infection with oocysts. Because few oocysts were found on the midgut of immune-activated mosquitoes, the data suggest that some factor, induced by bacterial challenge, kills the parasite at a preoocyst stage.},
keywords = {Aedes, Animals, Anopheles, Culicidae, Defensins, Digestive System, Escherichia coli, Female, Genetic, Hemolymph, hoffmann, Insect Vectors, M3i, messenger, Micrococcus luteus, Plasmodium, Plasmodium berghei, Plasmodium gallinaceum, Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Transcription},
pubstate = {published},
tppubtype = {article}
}
Ferrandon Dominique, Jung Alain C, Criqui M, Lemaitre Bruno, Uttenweiler-Joseph S, Michaut Lydia, Reichhart Jean-Marc, Hoffmann Jules A
A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway Article de journal
Dans: EMBO J., vol. 17, no. 5, p. 1217–1227, 1998, ISSN: 0261-4189.
Résumé | Liens | BibTeX | Étiquettes: Animals, bacteria, Cell Surface, Developmental, Digestive System, Epithelium, Fat Body, Female, ferrandon, Fungal, Gene Expression Regulation, Genes, Green Fluorescent Proteins, hoffmann, Insect Proteins, Larva, Luminescent Proteins, M3i, Male, Membrane Glycoproteins, Organ Specificity, Receptors, reichhart, Reporter, Respiratory System, Spores, Toll-Like Receptors, Trachea, Transgenes
@article{ferrandon_drosomycin-gfp_1998,
title = {A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway},
author = {Dominique Ferrandon and Alain C Jung and M Criqui and Bruno Lemaitre and S Uttenweiler-Joseph and Lydia Michaut and Jean-Marc Reichhart and Jules A Hoffmann},
doi = {10.1093/emboj/17.5.1217},
issn = {0261-4189},
year = {1998},
date = {1998-08-01},
journal = {EMBO J.},
volume = {17},
number = {5},
pages = {1217--1227},
abstract = {A hallmark of the systemic antimicrobial response of Drosophila is the synthesis by the fat body of several antimicrobial peptides which are released into the hemolymph in response to a septic injury. One of these peptides, drosomycin, is active primarily against fungi. Using a drosomycin-green fluorescent protein (GFP) reporter gene, we now show that in addition to the fat body, a variety of epithelial tissues that are in direct contact with the external environment, including those of the respiratory, digestive and reproductive tracts, can express the antifungal peptide, suggesting a local response to infections affecting these barrier tissues. As is the case for vertebrate epithelia, insect epithelia appear to be more than passive physical barriers and are likely to constitute an active component of innate immunity. We also show that, in contrast to the systemic antifungal response, this local immune response is independent of the Toll pathway.},
keywords = {Animals, bacteria, Cell Surface, Developmental, Digestive System, Epithelium, Fat Body, Female, ferrandon, Fungal, Gene Expression Regulation, Genes, Green Fluorescent Proteins, hoffmann, Insect Proteins, Larva, Luminescent Proteins, M3i, Male, Membrane Glycoproteins, Organ Specificity, Receptors, reichhart, Reporter, Respiratory System, Spores, Toll-Like Receptors, Trachea, Transgenes},
pubstate = {published},
tppubtype = {article}
}
Mézière C, Viguier M, Dumortier H, Lo-Man R, Leclerc C, Guillet J G, Briand J P, Muller S
In vivo Ŧ helper cell response to retro-inverso peptidomimetics Article de journal
Dans: Journal of Immunology (Baltimore, Md.: 1950), vol. 159, no. 7, p. 3230–3237, 1997, ISSN: 0022-1767.
Résumé | BibTeX | Étiquettes: Amino Acid Sequence, Animals, Antibodies, Antigen, Capsid, Capsid Proteins, Dumortier, Female, Helper-Inducer, Histocompatibility Antigens Class II, I2CT, Immunoglobulin Allotypes, Immunoglobulin G, Inbred BALB C, Injections, Intraperitoneal, Lymphocyte Activation, Mice, Molecular Sequence Data, Peptide Fragments, Poliovirus, Protein Binding, Receptors, T-Cell, T-Lymphocytes, Team-Dumortier, Viral
@article{meziere_vivo_1997,
title = {In vivo Ŧ helper cell response to retro-inverso peptidomimetics},
author = {C Mézière and M Viguier and H Dumortier and R Lo-Man and C Leclerc and J G Guillet and J P Briand and S Muller},
issn = {0022-1767},
year = {1997},
date = {1997-10-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {159},
number = {7},
pages = {3230--3237},
abstract = {Peptide analogues containing reversed peptide bonds between each residue along the peptide sequence (retro-inverso modification) have been analyzed for their antigenic and in vivo immunogenic properties in the MHC II and Th cell response context. Two antigenic peptides were selected for this study, namely peptide 103-115 of poliovirus VP1, which is involved in the production of Abs that neutralize the infectivity of the virus, and peptide 435-446 from the third constant region of mouse heavy chain IgG2a allopeptide gamma 2ab, which mimics a corneal Ag implicated in autoimmune keratitis. In a competition assay performed in vitro using reference hybridomas of known MHC class II restriction, both retro-inverso analogues bound (although more weakly in our test) to I-Ad and/or I-Ed class II molecules. However, in both cases, this lower affinity was apparently largely compensated in vivo, as a T cell response (with IL-2 secretion), equivalent to that obtained with the wild-type peptides, was observed following immunization of BALB/c mice with the retro-inverso analogues. Moreover, these T cells proliferated and produced IL-2 in response to the cognate peptides. It is concluded that the T cell receptors of T cells primed in vivo with the retro-inverso analogues readily cross-react with parent and retro-inverso analogue-MHC complexes. The approach of using pseudopeptides containing changes involving the backbone, and not the orientation of side chains, may thus be promising to design potent immunogens for class II-restricted T cells.},
keywords = {Amino Acid Sequence, Animals, Antibodies, Antigen, Capsid, Capsid Proteins, Dumortier, Female, Helper-Inducer, Histocompatibility Antigens Class II, I2CT, Immunoglobulin Allotypes, Immunoglobulin G, Inbred BALB C, Injections, Intraperitoneal, Lymphocyte Activation, Mice, Molecular Sequence Data, Peptide Fragments, Poliovirus, Protein Binding, Receptors, T-Cell, T-Lymphocytes, Team-Dumortier, Viral},
pubstate = {published},
tppubtype = {article}
}
Richman A M, Bulet Philippe, Hetru Charles, Barillas-Mury Carolina, Hoffmann Jules A, Kafalos Fotis C
Inducible immune factors of the vector mosquito Anopheles gambiae: biochemical purification of a defensin antibacterial peptide and molecular cloning of preprodefensin cDNA Article de journal
Dans: Insect Mol. Biol., vol. 5, no. 3, p. 203–210, 1996, ISSN: 0962-1075.
Résumé | BibTeX | Étiquettes: Amino Acid, Animals, Anopheles, Base Sequence, Blood Bactericidal Activity, Blood Proteins, Cloning, Complementary, Defensins, DNA, Escherichia coli, Female, Gene Expression, Genes, hoffmann, Insect, Insect Vectors, Larva, M3i, Micrococcus luteus, Molecular, Sequence Homology
@article{richman_inducible_1996,
title = {Inducible immune factors of the vector mosquito Anopheles gambiae: biochemical purification of a defensin antibacterial peptide and molecular cloning of preprodefensin cDNA},
author = {A M Richman and Philippe Bulet and Charles Hetru and Carolina Barillas-Mury and Jules A Hoffmann and Fotis C Kafalos},
issn = {0962-1075},
year = {1996},
date = {1996-08-01},
journal = {Insect Mol. Biol.},
volume = {5},
number = {3},
pages = {203--210},
abstract = {Larvae of the mosquito vector of human malaria, Anopheles gambiae, were inoculated with bacteria and extracts were biochemically fractionated by reverse-phase HPLC. Multiple induced polypeptides and antibacterial activities were observed following bacterial infection, including a member of the insect defensin family of antibacterial proteins. A cDNA encoding An. gambiae preprodefensin was isolated using PCR primers based on phylogenetically conserved sequences. The mature peptide is highly conserved, but the signal and propeptide segments are not, relative to corresponding defensin sequences of other insects. Defensin expression is induced in response to bacterial infection, in both adult and larval stages. In contrast, pupae express defensin mRNA constitutively. Defensin expression may prove a valuable molecular marker to monitor the An. gambiae host response to infection by parasitic protozoa of medical importance.},
keywords = {Amino Acid, Animals, Anopheles, Base Sequence, Blood Bactericidal Activity, Blood Proteins, Cloning, Complementary, Defensins, DNA, Escherichia coli, Female, Gene Expression, Genes, hoffmann, Insect, Insect Vectors, Larva, M3i, Micrococcus luteus, Molecular, Sequence Homology},
pubstate = {published},
tppubtype = {article}
}
Dirheimer G., Baranowski W., Keith G.
Variations in tRNA modifications, particularly of their queuine content in higher eukaryotes. Its relation to malignancy grading Article de journal
Dans: Biochimie, vol. 77, no. 1-2, p. 99-103, 1995, (0300-9084 Journal Article Review Review, Tutorial).
Résumé | BibTeX | Étiquettes: &, Animals, Cell, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Neoplasms/*genetics/pathology, Neoplastic/genetics, Non-U.S., Ovarian, post-transcriptional, Processing, Purines/analysis, Pyrimidines/analysis, RNA, Support, Transfer/*chemistry/metabolism, Transformation
@article{,
title = {Variations in tRNA modifications, particularly of their queuine content in higher eukaryotes. Its relation to malignancy grading},
author = { G. Dirheimer and W. Baranowski and G. Keith},
year = {1995},
date = {1995-01-01},
journal = {Biochimie},
volume = {77},
number = {1-2},
pages = {99-103},
abstract = {Literature references dealing with the variations in the modification level of nucleosides in total eukaryotic tRNAs as a function of different physiological status and after drug administration as well as in sequenced cytoplasmic tRNAs between normal and tumor cells and in SV40-transformed cells are reviewed. In addition, special attention is given to guanine replacement of queuine in the first position of the anticodon of tRNAs. A correlation between the level of this undermodification in cancer tissues and the malignancy grading could be found in human ovarian tumors, confirming the results reported in several laboratories for lymphomas and lung cancer tissues. Indeed tRNAs from primary and metastatic human ovarian malignant tumors are Q deficient as compared to tRNAs from normal tissues or benign tumors: thus queuine deficiency increases with malignancy and grading of differentiation.},
note = {0300-9084
Journal Article
Review
Review, Tutorial},
keywords = {&, Animals, Cell, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Neoplasms/*genetics/pathology, Neoplastic/genetics, Non-U.S., Ovarian, post-transcriptional, Processing, Purines/analysis, Pyrimidines/analysis, RNA, Support, Transfer/*chemistry/metabolism, Transformation},
pubstate = {published},
tppubtype = {article}
}
Meister Marie, Braun A, Kappler Christine, Reichhart Jean-Marc, Hoffmann Jules A
Insect immunity. A transgenic analysis in Drosophila defines several functional domains in the diptericin promoter Article de journal
Dans: EMBO J., vol. 13, no. 24, p. 5958–5966, 1994, ISSN: 0261-4189.
Résumé | BibTeX | Étiquettes: Animals, Anti-Infective Agents, Base Sequence, beta-Galactosidase, DNA Mutational Analysis, Female, Gene Expression Regulation, Genetic, Genetically Modified, Germ Cells, hoffmann, Insect Hormones, Insect Proteins, M3i, Male, Models, Nucleic Acid, Promoter Regions, Recombinant Fusion Proteins, reichhart, Repetitive Sequences, Transformation
@article{meister_insect_1994,
title = {Insect immunity. A transgenic analysis in Drosophila defines several functional domains in the diptericin promoter},
author = {Marie Meister and A Braun and Christine Kappler and Jean-Marc Reichhart and Jules A Hoffmann},
issn = {0261-4189},
year = {1994},
date = {1994-12-01},
journal = {EMBO J.},
volume = {13},
number = {24},
pages = {5958--5966},
abstract = {Diptericins are antibacterial polypeptides which are strongly induced in the fat body and blood cells of dipteran insects in response to septic injury. The promoter of the single-copy, intronless diptericin gene of Drosophila contains several nucleotide sequences homologous to mammalian cis-regulatory motifs involved in the control of acute phase response genes. Extending our previous studies on the expression of the diptericin gene, we now report a quantitative analysis of the contribution of various putative regulatory elements to the bacterial inducibility of this gene, based on the generation of 60 transgenic fly lines carrying different elements fused to a reporter gene. Our data definitively identify two Kappa B-related motifs in the proximal promoter as the sites conferring inducibility and tissue-specific expression to the diptericin gene. These motifs alone, however, mediate only minimal levels of expression. Additional proximal regulatory elements are necessary to attain some 20% of the full response and we suspect a role for sequences homologous to mammalian IL6 response elements and interferon-gamma responsive sites in this up-regulation. The transgenic experiments also reveal the existence of a distal regulatory element located upstream of -0.6 kb which increases the level of expression by a factor of five.},
keywords = {Animals, Anti-Infective Agents, Base Sequence, beta-Galactosidase, DNA Mutational Analysis, Female, Gene Expression Regulation, Genetic, Genetically Modified, Germ Cells, hoffmann, Insect Hormones, Insect Proteins, M3i, Male, Models, Nucleic Acid, Promoter Regions, Recombinant Fusion Proteins, reichhart, Repetitive Sequences, Transformation},
pubstate = {published},
tppubtype = {article}
}
Baranowski W., Dirheimer G., Jakowicki J. A., Keith G.
Deficiency of queuine, a highly modified purine base, in transfer RNAs from primary and metastatic ovarian malignant tumors in women Article de journal
Dans: Cancer Res, vol. 54, no. 16, p. 4468-71, 1994, (0008-5472 Journal Article).
Résumé | BibTeX | Étiquettes: &, Adolescent, Adult, Aged, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Middle, Neoplasm/*chemistry, Neoplasms/*chemistry/pathology, Non-U.S., Ovarian, RNA, Support, Transfer/*chemistry
@article{,
title = {Deficiency of queuine, a highly modified purine base, in transfer RNAs from primary and metastatic ovarian malignant tumors in women},
author = { W. Baranowski and G. Dirheimer and J. A. Jakowicki and G. Keith},
year = {1994},
date = {1994-01-01},
journal = {Cancer Res},
volume = {54},
number = {16},
pages = {4468-71},
abstract = {The tRNAs from rapidly growing tissues, particularly from neoplasia, often exhibit queuine deficiency. In order to check whether different kinds of ovarian tumors display queuine deficiencies we have analyzed tRNA samples from 16 ovarian malignancies. The tRNAs from histologically normal myometrium (4 samples) and myoma (6 samples) were taken as healthy tissue and benign tumor references. Queuine deficiency was determined by an exchange assay using [8-3H]guanine and tRNA:guanine transglycosylase from Escherichia coli. The mean values of queuine deficiencies in tRNAs were: 10.95 +/- 2.21 (SD) pmol/A260 in gonadal and germ cell tumors (5 cases); 23.75 +/- 7.89 pmol/A260 in primary epithelial tumors (9 cases); and 34.58 +/- 7.18 pmol/A260 in metastatic tumors (2 cases). These values displayed statistically significant differences (P = 0.0003, Kruskal-Wallis test). The queuine deficiencies in tRNAs significantly increased when moving from well-differentiated through moderately differentiated to poorly differentiated tumors, with the highest values found in poorly differentiated metastatic tumors (P = 0.0002, Kruskal-Wallis test). Queuine deficiency determination in tRNAs is proposed as a factor for clinical outcome prognosis of ovarian malignancies.},
note = {0008-5472
Journal Article},
keywords = {&, Adolescent, Adult, Aged, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Middle, Neoplasm/*chemistry, Neoplasms/*chemistry/pathology, Non-U.S., Ovarian, RNA, Support, Transfer/*chemistry},
pubstate = {published},
tppubtype = {article}
}
Baranowski W., Tomaszewski J., Keith G.
[Methylation of DNA in tissue of ovarian malignant tumors in women] Article de journal
Dans: Ginekol Pol, vol. 64, no. 4, p. 169-73, 1993, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: Abstract, Adult, Aged, Carcinoma/genetics, Cell, DNA, English, Female, Human, Methylation, Middle, Neoplasm/*metabolism, Neoplasms/*genetics, Ovarian, Sertoli, Tumor/genetics
@article{,
title = {[Methylation of DNA in tissue of ovarian malignant tumors in women]},
author = { W. Baranowski and J. Tomaszewski and G. Keith},
year = {1993},
date = {1993-01-01},
journal = {Ginekol Pol},
volume = {64},
number = {4},
pages = {169-73},
abstract = {DNA methylation level from woman ovarian malignant tissues was investigated by 32P postlabeling of the single nucleotides, separation on TLC with followed autoradiography and Cerenkov counting. Slight differences in DNA methylation extent were found in malignant ovarian tumors as compared to normal myometrium and benign uterine tumor [myomas]. Lowest level of m5dC in relation to C and also in relation to total DNA was found in carcinoma embryonal tissue whereas maximal DNA methylation level was obtained in folliculoma tissue. This preliminary report needs further investigation of particularly genes methylation.},
note = {0017-0011
Journal Article},
keywords = {Abstract, Adult, Aged, Carcinoma/genetics, Cell, DNA, English, Female, Human, Methylation, Middle, Neoplasm/*metabolism, Neoplasms/*genetics, Ovarian, Sertoli, Tumor/genetics},
pubstate = {published},
tppubtype = {article}
}
Baranowski W., Tomaszewski J., Keith G.
Unusual deficiency of the modified purine base queuine in transfer ribonucleic acid from the human placenta as tested by enzymatic assay Article de journal
Dans: Am J Obstet Gynecol, vol. 169, no. 3, p. 581-2, 1993, (0002-9378 Journal Article).
Résumé | BibTeX | Étiquettes: &, derivatives/chemistry, Female, Guanine/*analogs, Human, Placenta/*chemistry, Pregnancy, RNA, Transfer/*chemistry
@article{,
title = {Unusual deficiency of the modified purine base queuine in transfer ribonucleic acid from the human placenta as tested by enzymatic assay},
author = { W. Baranowski and J. Tomaszewski and G. Keith},
year = {1993},
date = {1993-01-01},
journal = {Am J Obstet Gynecol},
volume = {169},
number = {3},
pages = {581-2},
abstract = {Transfer ribonucleic acid from rapidly growing tissues, particularly from neoplasia, is partially deficient in queuine, a highly modified transfer ribonucleic acid constituent. By means of an enzymatic assay we also found a queuine deficiency (14%) in human placenta transfer ribonucleic acid despite its high concentrations in the amniotic fluid. Proposed cause and significance of the results are discussed.},
note = {0002-9378
Journal Article},
keywords = {&, derivatives/chemistry, Female, Guanine/*analogs, Human, Placenta/*chemistry, Pregnancy, RNA, Transfer/*chemistry},
pubstate = {published},
tppubtype = {article}
}
Hetru Charles, Li K W, Bulet Philippe, Lagueux Marie, Hoffmann Jules A
Isolation and structural characterization of an insulin-related molecule, a predominant neuropeptide from Locusta migratoria Article de journal
Dans: Eur. J. Biochem., vol. 201, no. 2, p. 495–499, 1991, ISSN: 0014-2956.
Résumé | BibTeX | Étiquettes: Animals, Chromatography, DNA, Female, Grasshoppers, High Pressure Liquid, hoffmann, Insect Hormones, Insulin, M3i, Mass Spectrometry, Neuropeptides, Proinsulin, Protein Conformation
@article{hetru_isolation_1991,
title = {Isolation and structural characterization of an insulin-related molecule, a predominant neuropeptide from Locusta migratoria},
author = {Charles Hetru and K W Li and Philippe Bulet and Marie Lagueux and Jules A Hoffmann},
issn = {0014-2956},
year = {1991},
date = {1991-10-01},
journal = {Eur. J. Biochem.},
volume = {201},
number = {2},
pages = {495--499},
abstract = {Neurohaemal lobes of corpora cardiaca of Locusta migratoria are an established storage site for neurohormones produced by the neurosecretory cells of the brain. As previously reported [Hietter, H., Van Dorsselaer, A., Green, B., Denoroy, L., Hoffmann, J.A. & Luu, B. (1990) Eur. J. Biochem. 187, 241-247], the isolation and characterization of a novel 5-kDa peptide from these lobes served as the basis for oligonucleotide screening of cDNA libraries prepared from poly(A) RNA from neurosecretory cells of the central nervous system. From subsequent cDNA cloning studies [Lagueux, M., Lwoff, L., Meister, M., Goltzené, F. & Hoffmann, J.A. (1990) Eur. J. Biochem. 187, 249-254], the existence of a 145-residue precursor protein was deduced, which contained, in addition to the 5-kDa peptide, amino-acid sequences with homology to the A and B chains of an insulin-related peptide. In the present study we have isolated the native molecule from corpora cardiaca of Locusta and characterized, by Edman degradation and plasma-desorption mass spectrometry, the two chains as follows: A chain, Gly-Val-Phe-Asp-Glu-Cys-Cys-Arg-Lys-Ser-Cys-Ser-Ile-Ser-Glu-Leu-Gln-Thr- Tyr-Cys - Gly (Ile, isoleucine); B chain, Ser-Gly-Ala-Pro-Gln-Pro-Val-Ala-Arg-Tyr-Cys-Gly-Glu-Lys-Leu-Ser-Asn-Ala- Leu-Lys - Leu-Val-Cys-Arg-Gly-Asn-Tyr-Asn-Thr-Met-Phe. Taken in conjunction with the previous cloning studies, our data lead to a clear picture of the processing of Locusta preproinsulin. They indicate that locusta corpora cardiaca contain remarkably large amounts of one single insulin form, in contrast to multiple insulin isoforms of Bombyx mori, the only other insect species from which insulin-related peptides have been isolated and characterized [Nagasawa, H., Kataoka, H., Isogai, A., Tamura, S., Suzuki, A., Mizoguchi, A., Fujiwara, Y., Suzuki, A., Takahashi, S. & Ishizaki, H. (1986) Proc. Natl Acad. Sci. USA 83, 5840-5843].},
keywords = {Animals, Chromatography, DNA, Female, Grasshoppers, High Pressure Liquid, hoffmann, Insect Hormones, Insulin, M3i, Mass Spectrometry, Neuropeptides, Proinsulin, Protein Conformation},
pubstate = {published},
tppubtype = {article}
}
Lanot R, Thiebold J, Costet-Corio M F, Benveniste P, Hoffmann Jules A
Further experimental evidence for the involvement of ecdysone in the control of meiotic reinitiation in oocytes of Locusta migratoria (Insecta, Orthoptera) Article de journal
Dans: Dev. Biol., vol. 126, no. 1, p. 212–214, 1988, ISSN: 0012-1606.
Résumé | BibTeX | Étiquettes: Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Meiosis, Oocytes, Ovary
@article{lanot_further_1988,
title = {Further experimental evidence for the involvement of ecdysone in the control of meiotic reinitiation in oocytes of Locusta migratoria (Insecta, Orthoptera)},
author = {R Lanot and J Thiebold and M F Costet-Corio and P Benveniste and Jules A Hoffmann},
issn = {0012-1606},
year = {1988},
date = {1988-03-01},
journal = {Dev. Biol.},
volume = {126},
number = {1},
pages = {212--214},
abstract = {Ecdysone has recently been shown to be able to trigger meiotic reinitiation in vitro in submature oocytes of Locusta. In the present study we have experimentally depressed (by 60-70%) ecdysone biosynthesis in the ovaries of adult females by rearing them on a diet with a modified sterol profile. Mature oocytes from such females fail to undergo normal reinitiation, but when incubated in vitro, can be induced to break their meiotic arrest by the addition of exogenous ecdysone. These results lend further support to the hypothesis that in Locusta, ovarian ecdysone is involved in the control of meiotic reinitiation.},
keywords = {Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Meiosis, Oocytes, Ovary},
pubstate = {published},
tppubtype = {article}
}
Tsoupras G, Luu B, Hoffmann Jules A
Isolation and identification of three ecdysteroid conjugates with a C-20 hydroxy group in eggs of Locusta migratoria Article de journal
Dans: Steroids, vol. 40, no. 5, p. 551–560, 1982, ISSN: 0039-128X.
Résumé | BibTeX | Étiquettes: Animals, Chromatography, Ecdysone, Ecdysterone, Female, Grasshoppers, High Pressure Liquid, hoffmann, M3i, Magnetic Resonance Spectroscopy, Ovum, Phosphates, Spectrophotometry, Ultraviolet
@article{tsoupras_isolation_1982,
title = {Isolation and identification of three ecdysteroid conjugates with a C-20 hydroxy group in eggs of Locusta migratoria},
author = {G Tsoupras and B Luu and Jules A Hoffmann},
issn = {0039-128X},
year = {1982},
date = {1982-11-01},
journal = {Steroids},
volume = {40},
number = {5},
pages = {551--560},
abstract = {Three ecdysteroids conjugates with a hydroxy group at C-20 were isolated from developing eggs of locusta migratoria and identified as 22-phosphate conjugates of 2-deoxy-20-hydroxy-ecdysone, 20-hydroxyecdysone and 20-hydroxyecdysone acetate.},
keywords = {Animals, Chromatography, Ecdysone, Ecdysterone, Female, Grasshoppers, High Pressure Liquid, hoffmann, M3i, Magnetic Resonance Spectroscopy, Ovum, Phosphates, Spectrophotometry, Ultraviolet},
pubstate = {published},
tppubtype = {article}
}
Lachaise F, Hoffmann Jules A
Ecdysteroids and embryonic development in the shore crab, Carcinus maenas Article de journal
Dans: Hoppe-Seyler's Z. Physiol. Chem., vol. 363, no. 9, p. 1059–1067, 1982, ISSN: 0018-4888.
Résumé | BibTeX | Étiquettes: Animals, Brachyura, Ecdysone, Ecdysterone, Embryo, Female, hoffmann, M3i, Nonmammalian, Ovum, Radioimmunoassay
@article{lachaise_ecdysteroids_1982,
title = {Ecdysteroids and embryonic development in the shore crab, Carcinus maenas},
author = {F Lachaise and Jules A Hoffmann},
issn = {0018-4888},
year = {1982},
date = {1982-09-01},
journal = {Hoppe-Seyler's Z. Physiol. Chem.},
volume = {363},
number = {9},
pages = {1059--1067},
abstract = {Eggs at various stages of embryonic development of Carcinus maenas contain high concentrations of the ecdysteroid ponasterone A together with lower titres of 20-hydroxyecdysone and ecdysone. Correlative studies on ecdysteroid titres and developmental characteristics of Carcinus embryos indicate that one function of ponasterone A might be related to the control of deposition of an embryonic envelope.},
keywords = {Animals, Brachyura, Ecdysone, Ecdysterone, Embryo, Female, hoffmann, M3i, Nonmammalian, Ovum, Radioimmunoassay},
pubstate = {published},
tppubtype = {article}
}
Hetru Charles, Kappler Christine, Hoffmann Jules A, Nearn R, Bang Lee, Horn D H
The biosynthetic pathway of ecdysone: studies with vitellogenic ovaries of Locusta migratoria (Orthoptera) Article de journal
Dans: Mol. Cell. Endocrinol., vol. 26, no. 1-2, p. 51–80, 1982, ISSN: 0303-7207.
Résumé | BibTeX | Étiquettes: Animals, Chromatography, Ecdysone, Female, Gas Chromatography-Mass Spectrometry, High Pressure Liquid, hoffmann, M3i, Orthoptera, Ovary
@article{hetru_biosynthetic_1982,
title = {The biosynthetic pathway of ecdysone: studies with vitellogenic ovaries of Locusta migratoria (Orthoptera)},
author = {Charles Hetru and Christine Kappler and Jules A Hoffmann and R Nearn and Lee Bang and D H Horn},
issn = {0303-7207},
year = {1982},
date = {1982-04-01},
journal = {Mol. Cell. Endocrinol.},
volume = {26},
number = {1-2},
pages = {51--80},
abstract = {Ovaries of adult females of Locusta migratoria synthesize impressive amounts of the steroid hormone ecdysone (and related ecdysteroids) during the late phases of vitellogenesis. The present study, aimed at elucidating the sequence of the biosynthetic steps that lead from cholesterol to ecdysone, has taken benefit of this remarkable biological model by using a double approach: (1) isolation and physico-chemical identification of endogenous biogenetic intermediates; (2) metabolic study of labelled putative precursor molecules. The data presented in this paper lead us to propose the following sequence of events: conversion of cholesterol to 3 beta-hydroxy-5 beta-cholest-7-en-6-one (via several intermediates not identified in this study) followed by 14 beta-hydroxylation to 3 beta, 14 alpha-dihydroxy-5 beta-cholest-7-en-6-one; hydroxylation on the side-chain at C-25 and C-22 (in this order) to 2-deoxyecdysone; hydroxylation at C-2 to ecdysone.},
keywords = {Animals, Chromatography, Ecdysone, Female, Gas Chromatography-Mass Spectrometry, High Pressure Liquid, hoffmann, M3i, Orthoptera, Ovary},
pubstate = {published},
tppubtype = {article}
}
Lagueux Marie, Harry P, Hoffmann Jules A
Ecdysteroids are bound to vitellin in newly laid eggs of Locusta Article de journal
Dans: Mol. Cell. Endocrinol., vol. 24, no. 3, p. 325–338, 1981, ISSN: 0303-7207.
Résumé | BibTeX | Étiquettes: Animals, Chromatography, Ecdysteroids, Female, Grasshoppers, High Pressure Liquid, hoffmann, Invertebrate Hormones, Lipoproteins, M3i, Molecular Weight, Ovum, Peptide Hydrolases, Radioimmunoassay, Vitellogenins
@article{lagueux_ecdysteroids_1981,
title = {Ecdysteroids are bound to vitellin in newly laid eggs of Locusta},
author = {Marie Lagueux and P Harry and Jules A Hoffmann},
issn = {0303-7207},
year = {1981},
date = {1981-12-01},
journal = {Mol. Cell. Endocrinol.},
volume = {24},
number = {3},
pages = {325--338},
abstract = {The follicle cells of vitellogenic ovaries of Locusta migratoria have been reported to synthesize impressive amounts of ecdysteroids which accumulate inside the oöcytes where they persist during egg-laying; these ecdysteroids are conjugated to more than 95%, and it is believed that their hydrolysis in the egg is the source of the peaks of free ecdysone observed in early embryonic development. The present paper shows that, in the eggs, the ecdysteroid conjugates are bound to a 520 000-dalton macromolecule which shares several characteristics with the major yolk protein vitellin and is precipitated by an anti-vitellin antibody. The physiological relevance of the binding of ovarian ecdysteroid conjugates is discussed in respect to the transfer of maternal ecdysteroids to the embryo.},
keywords = {Animals, Chromatography, Ecdysteroids, Female, Grasshoppers, High Pressure Liquid, hoffmann, Invertebrate Hormones, Lipoproteins, M3i, Molecular Weight, Ovum, Peptide Hydrolases, Radioimmunoassay, Vitellogenins},
pubstate = {published},
tppubtype = {article}
}
Lachaise F, Goudeau M, Hetru Charles, Kappler Christine, Hoffmann Jules A
Ecdysteroids and ovarian development in the shore crab, Carcinus maenas Article de journal
Dans: Hoppe-Seyler's Z. Physiol. Chem., vol. 362, no. 5, p. 521–529, 1981, ISSN: 0018-4888.
Résumé | BibTeX | Étiquettes: aging, Animals, Brachyura, Cross Reactions, Ecdysone, Ecdysterone, Female, hoffmann, M3i, Ovary, Radioimmunoassay, Sexual Maturation
@article{lachaise_ecdysteroids_1981,
title = {Ecdysteroids and ovarian development in the shore crab, Carcinus maenas},
author = {F Lachaise and M Goudeau and Charles Hetru and Christine Kappler and Jules A Hoffmann},
issn = {0018-4888},
year = {1981},
date = {1981-01-01},
journal = {Hoppe-Seyler's Z. Physiol. Chem.},
volume = {362},
number = {5},
pages = {521--529},
abstract = {Mature ovaries of the shore crab Carcinus maenas contain large concentrations of three major ecdysteroids which we have identified by physicochemical methods as ecdysone, 20-hydroxyecdysone and ponasterone A. The fluctuations of ovarian and blood ecdysteroid concentrations are presented in relation to the various stages of ovarian development.},
keywords = {aging, Animals, Brachyura, Cross Reactions, Ecdysone, Ecdysterone, Female, hoffmann, M3i, Ovary, Radioimmunoassay, Sexual Maturation},
pubstate = {published},
tppubtype = {article}
}
Goltzené F, Lagueux Marie, Charlet Maurice, Hoffmann Jules A
The follicle cell epithelium of maturing ovaries of Locusta migratoria: a new biosynthetic tissue for ecdysone Article de journal
Dans: Hoppe-Seyler's Z. Physiol. Chem., vol. 359, no. 10, p. 1427–1434, 1978, ISSN: 0018-4888.
Résumé | BibTeX | Étiquettes: Animals, Ecdysone, Epithelium, Female, Grasshoppers, hoffmann, Kinetics, M3i, Oocytes, Ovarian Follicle, Ovary, Sexual Maturation
@article{goltzene_follicle_1978,
title = {The follicle cell epithelium of maturing ovaries of Locusta migratoria: a new biosynthetic tissue for ecdysone},
author = {F Goltzené and Marie Lagueux and Maurice Charlet and Jules A Hoffmann},
issn = {0018-4888},
year = {1978},
date = {1978-10-01},
journal = {Hoppe-Seyler's Z. Physiol. Chem.},
volume = {359},
number = {10},
pages = {1427--1434},
abstract = {Follicle cells of maturing ovaries of Locusta migratoria are demonstrated to synthesize the moulting hormone ecdysone (2beta,3beta,14alpha,22R,25-pentahydroxy-5beta-cholest-7-en-6-one). Studies of secretory kinetics under in vitro conditions show that the intensity of hormone secretion is strictly dependent on the stage of maturation of the excised ovaries.},
keywords = {Animals, Ecdysone, Epithelium, Female, Grasshoppers, hoffmann, Kinetics, M3i, Oocytes, Ovarian Follicle, Ovary, Sexual Maturation},
pubstate = {published},
tppubtype = {article}
}
Hetru Charles, Lagueux Marie, Bang L, Hoffmann Jules A
Adult ovaries of Locusta migratoria contain the sequence of biosynthetic intermediates for ecdysone Article de journal
Dans: Life Sci., vol. 22, no. 23, p. 2141–2154, 1978, ISSN: 0024-3205.
BibTeX | Étiquettes: Animals, Chemical Phenomena, Chemistry, Cholesterol, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary, Ovum
@article{hetru_adult_1978,
title = {Adult ovaries of Locusta migratoria contain the sequence of biosynthetic intermediates for ecdysone},
author = {Charles Hetru and Marie Lagueux and L Bang and Jules A Hoffmann},
issn = {0024-3205},
year = {1978},
date = {1978-06-01},
journal = {Life Sci.},
volume = {22},
number = {23},
pages = {2141--2154},
keywords = {Animals, Chemical Phenomena, Chemistry, Cholesterol, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary, Ovum},
pubstate = {published},
tppubtype = {article}
}
Lagueux Marie, Hirn M, Hoffmann Jules A
Ecdysone during ovarian development in Locusta migratoria Article de journal
Dans: J. Insect Physiol., vol. 23, no. 1, p. 109–119, 1977, ISSN: 0022-1910.
BibTeX | Étiquettes: Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Male, Oogenesis, Ovary
@article{lagueux_ecdysone_1977,
title = {Ecdysone during ovarian development in Locusta migratoria},
author = {Marie Lagueux and M Hirn and Jules A Hoffmann},
issn = {0022-1910},
year = {1977},
date = {1977-01-01},
journal = {J. Insect Physiol.},
volume = {23},
number = {1},
pages = {109--119},
keywords = {Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Male, Oogenesis, Ovary},
pubstate = {published},
tppubtype = {article}
}
Bang L, Lagueux Marie, Hirn M, Hoffmann Jules A
Identification, by gas chromatography and mass spectrometry, of ecdysone synthesized in the ovaries of adult female Locusta migratoria (Insecta, Orthoptera) Article de journal
Dans: C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat., vol. 283, no. 9, p. 1081–1084, 1976.
Résumé | BibTeX | Étiquettes: Age Factors, Animals, Chromatography, Ecdysone, Female, Gas, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary
@article{bang_identification_1976,
title = {Identification, by gas chromatography and mass spectrometry, of ecdysone synthesized in the ovaries of adult female Locusta migratoria (Insecta, Orthoptera)},
author = {L Bang and Marie Lagueux and M Hirn and Jules A Hoffmann},
year = {1976},
date = {1976-10-01},
journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},
volume = {283},
number = {9},
pages = {1081--1084},
abstract = {Gas chromatographic analysis, coupled to mass spectrometry, demonstrates that the considerable amounts of ecdysteroids produced in the ovaries of female adults of Locusta migratoria at the end of each ovarian cycle, consist predominantly of ecdysone.},
keywords = {Age Factors, Animals, Chromatography, Ecdysone, Female, Gas, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary},
pubstate = {published},
tppubtype = {article}
}
Lagueux Marie, Hirn M, de Reggi M, Hoffmann Jules A
Ecdysteroid levels and ovarian development in adult female Locust migratoria Article de journal
Dans: C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat., vol. 282, no. 12, p. 1187–1190, 1976.
Résumé | BibTeX | Étiquettes: Adipose Tissue, Animals, Castration, Female, Grasshoppers, Hemolymph, hoffmann, Invertebrate Hormones, M3i, Neurosecretory Systems, Oogenesis, Ovary, Oviposition
@article{lagueux_ecdysteroid_1976,
title = {Ecdysteroid levels and ovarian development in adult female Locust migratoria},
author = {Marie Lagueux and M Hirn and M de Reggi and Jules A Hoffmann},
year = {1976},
date = {1976-03-01},
journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},
volume = {282},
number = {12},
pages = {1187--1190},
abstract = {During ovarian development in adult females of Locusta, an important biosynthesis of ecdysteroids is observed at the end of the maturation process of the terminal oocyte. This phenomenon occurs regularly during the different ovarian cycles. No synthesis is observed in allatectomized or ovariectomized female adults.},
keywords = {Adipose Tissue, Animals, Castration, Female, Grasshoppers, Hemolymph, hoffmann, Invertebrate Hormones, M3i, Neurosecretory Systems, Oogenesis, Ovary, Oviposition},
pubstate = {published},
tppubtype = {article}
}
Goltzené F, Hoffmann Jules A
Control of haemolymph protein synthesis and oocyte maturation by the corpora allata in female adults of locusta migratoria (Orthoptera): role of the blood-forming tissue Article de journal
Dans: Gen. Comp. Endocrinol., vol. 22, no. 4, p. 489–498, 1974, ISSN: 0016-6480.
BibTeX | Étiquettes: Animals, Disc, Electrophoresis, Female, Grasshoppers, Hematopoietic System, Hemolymph, hoffmann, M3i, Ovum, Protein Biosynthesis, Radiation Effects
@article{goltzene_control_1974,
title = {Control of haemolymph protein synthesis and oocyte maturation by the corpora allata in female adults of locusta migratoria (Orthoptera): role of the blood-forming tissue},
author = {F Goltzené and Jules A Hoffmann},
issn = {0016-6480},
year = {1974},
date = {1974-04-01},
journal = {Gen. Comp. Endocrinol.},
volume = {22},
number = {4},
pages = {489--498},
keywords = {Animals, Disc, Electrophoresis, Female, Grasshoppers, Hematopoietic System, Hemolymph, hoffmann, M3i, Ovum, Protein Biosynthesis, Radiation Effects},
pubstate = {published},
tppubtype = {article}
}
Hoffmann Jules A
Endocrine regulation of production and differentiation of hemocytes in an orthopteran insect: Locusta migratoria migratoroides Article de journal
Dans: Gen. Comp. Endocrinol., vol. 15, no. 2, p. 198–219, 1970, ISSN: 0016-6480.
BibTeX | Étiquettes: Animals, Biological, Blood Cells, Electrocoagulation, Female, Hemolymph, hoffmann, insects, M3i, Male, Metamorphosis, Microscopy, Neurosecretory Systems, Phase-Contrast
@article{hoffmann_endocrine_1970,
title = {Endocrine regulation of production and differentiation of hemocytes in an orthopteran insect: Locusta migratoria migratoroides},
author = {Jules A Hoffmann},
issn = {0016-6480},
year = {1970},
date = {1970-10-01},
journal = {Gen. Comp. Endocrinol.},
volume = {15},
number = {2},
pages = {198--219},
keywords = {Animals, Biological, Blood Cells, Electrocoagulation, Female, Hemolymph, hoffmann, insects, M3i, Male, Metamorphosis, Microscopy, Neurosecretory Systems, Phase-Contrast},
pubstate = {published},
tppubtype = {article}
}