Publications
2015
Aguiar Eric Roberto Guimarães Rocha, Olmo Roenick Proveti, Paro Simona, Ferreira Flavia Viana, da de Faria Isaque João Silva, Todjro Yaovi Mathias Honore, Lobo Francisco Pereira, Kroon Erna Geessien, Meignin Carine, Gatherer Derek, Imler Jean-Luc, Marques João Trindade
Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host Article de journal
Dans: Nucleic Acids Research, vol. 43, no. 13, p. 6191–6206, 2015, ISSN: 1362-4962.
Résumé | Liens | BibTeX | Étiquettes: Animals, Contig Mapping, Female, imler, insects, M3i, meignin, Ovary, Plants, RNA, Sequence Analysis, Small Untranslated, Vertebrates, Viral, Viral Tropism, viruses
@article{aguiar_sequence-independent_2015,
title = {Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host},
author = {Eric Roberto Guimarães Rocha Aguiar and Roenick Proveti Olmo and Simona Paro and Flavia Viana Ferreira and Isaque João Silva da de Faria and Yaovi Mathias Honore Todjro and Francisco Pereira Lobo and Erna Geessien Kroon and Carine Meignin and Derek Gatherer and Jean-Luc Imler and João Trindade Marques},
url = {http://nar.oxfordjournals.org/lookup/doi/10.1093/nar/gkv587},
doi = {10.1093/nar/gkv587},
issn = {1362-4962},
year = {2015},
date = {2015-07-01},
journal = {Nucleic Acids Research},
volume = {43},
number = {13},
pages = {6191--6206},
abstract = {Virus surveillance in vector insects is potentially of great benefit to public health. Large-scale sequencing of small and long RNAs has previously been used to detect viruses, but without any formal comparison of different strategies. Furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. Here, we developed a sequence-independent strategy based on virus-derived small RNAs produced by the host response, such as the RNA interference pathway. In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. Using this strategy, we characterized six novel viruses in the viromes of laboratory fruit flies and wild populations of two insect vectors: mosquitoes and sandflies. We also show that the small RNA profile could be used to infer viral tropism for ovaries among other aspects of virus biology. Additionally, our results suggest that virus detection utilizing small RNAs can also be applied to vertebrates, although not as efficiently as to plants and insects.},
keywords = {Animals, Contig Mapping, Female, imler, insects, M3i, meignin, Ovary, Plants, RNA, Sequence Analysis, Small Untranslated, Vertebrates, Viral, Viral Tropism, viruses},
pubstate = {published},
tppubtype = {article}
}
Paro Simona, Imler Jean-Luc, Meignin Carine
Sensing viral RNAs by Dicer/RIG-I like ATPases across species Article de journal
Dans: Current Opinion in Immunology, vol. 32, p. 106–113, 2015, ISSN: 1879-0372.
Résumé | Liens | BibTeX | Étiquettes: Adenosine Triphosphatases, Animals, DEAD-box RNA Helicases, Humans, imler, M3i, meignin, Protein Binding, Protein Interaction Domains and Motifs, Ribonuclease III, RNA, Viral, Virus Diseases, viruses
@article{paro_sensing_2015,
title = {Sensing viral RNAs by Dicer/RIG-I like ATPases across species},
author = {Simona Paro and Jean-Luc Imler and Carine Meignin},
url = {http://linkinghub.elsevier.com/retrieve/pii/S0952791515000102},
doi = {10.1016/j.coi.2015.01.009},
issn = {1879-0372},
year = {2015},
date = {2015-02-01},
journal = {Current Opinion in Immunology},
volume = {32},
pages = {106--113},
abstract = {Induction of antiviral immunity in vertebrates and invertebrates relies on members of the RIG-I-like receptor and Dicer families, respectively. Although these proteins have different size and domain composition, members of both families share a conserved DECH-box helicase domain. This helicase, also known as a duplex RNA activated ATPase, or DRA domain, plays an important role in viral RNA sensing. Crystallographic and electron microscopy studies of the RIG-I and Dicer DRA domains indicate a common structure and that similar conformational changes are induced by dsRNA binding. Genetic and biochemical studies on the function and regulation of DRAs reveal similarities, but also some differences, between viral RNA sensing mechanisms in nematodes, flies and mammals.},
keywords = {Adenosine Triphosphatases, Animals, DEAD-box RNA Helicases, Humans, imler, M3i, meignin, Protein Binding, Protein Interaction Domains and Motifs, Ribonuclease III, RNA, Viral, Virus Diseases, viruses},
pubstate = {published},
tppubtype = {article}
}
2011
Chtarbanova Stanislava, Imler Jean-Luc
Immunité innée antivirale chez la drosophile Article de journal
Dans: Virologie, vol. 15, no. 5, p. 296-306, 2011, ISSN: 1267-8694.
Résumé | Liens | BibTeX | Étiquettes: *RNA Interference, antiviral immunity, Argonaute, dicer, helicase, imler, M3i, viruses
@article{Chtarbanova2011,
title = {Immunité innée antivirale chez la drosophile},
author = {Stanislava Chtarbanova and Jean-Luc Imler},
url = {http://www.jle.com/fr/revues/vir/e-docs/immunite_innee_antivirale_chez_la_drosophile_290366/article.phtml?tab=texte},
doi = {10.1684/vir.2011.0417},
issn = {1267-8694},
year = {2011},
date = {2011-10-01},
journal = {Virologie},
volume = {15},
number = {5},
pages = {296-306},
abstract = {La drosophile est utilisée depuis une quinzaine d’années comme modèle pour l’étude des mécanismes de l’immunité innée contre les infections bactériennes et fongiques. Les mécanismes de défense de cet insecte contre les infections virales sont maintenant abordés. L’interférence ARN joue un rôle essentiel dans la détection et le contrôle des virus. Ce mécanisme implique la reconnaissance des ARN double brins viraux par Dicer-2 et leur coupure pour former des petits ARN interférants (ARNsi) de 21 nucléotides. Ces ARNsi sont ensuite chargés sur l’enzyme Argonaute-2, qui inhibera spécifiquement les ARN viraux contenant une séquence complémentaire. Une réponse immunitaire inductible contribue également au contrôle des infections virales chez la drosophile. Cette réponse implique les voies de signalisation Toll et IMD, d’une part, (régulant des facteurs de transcription de la famille NF-κB) et JAK/STAT, d’autre part. On ne sait encore que peu de choses sur la nature des molécules effectrices régulées par ces voies de signalisation et sur les récepteurs qui les activent. Même si de nombreux points d’interrogation demeurent, l’intérêt du modèle drosophile pour identifier des mécanismes de défense antivirale conservés au cours de l’évolution, et impliqués dans la transmission des arbovirus par les moustiques, est aujourd’hui bien établi.},
keywords = {*RNA Interference, antiviral immunity, Argonaute, dicer, helicase, imler, M3i, viruses},
pubstate = {published},
tppubtype = {article}
}
2007
Beutler Bruce, Eidenschenk Celine, Crozat Karine, Imler Jean-Luc, Takeuchi Osamu, Hoffmann Jules A, Akira Shizuo
Genetic analysis of resistance to viral infection Article de journal
Dans: Nature Reviews. Immunology, vol. 7, no. 10, p. 753–766, 2007, ISSN: 1474-1741.
Résumé | Liens | BibTeX | Étiquettes: Animals, Antiviral Agents, Disease Susceptibility, Drug Resistance, Eukaryotic Cells, hoffmann, Humans, imler, Immunity, M3i, Mutation, Viral, Virus Diseases, viruses
@article{beutler_genetic_2007,
title = {Genetic analysis of resistance to viral infection},
author = {Bruce Beutler and Celine Eidenschenk and Karine Crozat and Jean-Luc Imler and Osamu Takeuchi and Jules A Hoffmann and Shizuo Akira},
doi = {10.1038/nri2174},
issn = {1474-1741},
year = {2007},
date = {2007-10-01},
journal = {Nature Reviews. Immunology},
volume = {7},
number = {10},
pages = {753--766},
abstract = {As machines that reprogramme eukaryotic cells to suit their own purposes, viruses present a difficult problem for multicellular hosts, and indeed, have become one of the central pre-occupations of the immune system. Unable to permanently outpace individual viruses in an evolutionary footrace, higher eukaryotes have evolved broadly active mechanisms with which to sense viruses and suppress their proliferation. These mechanisms have recently been elucidated by a combination of forward and reverse genetic methods. Some of these mechanisms are clearly ancient, whereas others are relatively new. All are remarkably adept at discriminating self from non-self, and allow the host to cope with what might seem an impossible predicament.},
keywords = {Animals, Antiviral Agents, Disease Susceptibility, Drug Resistance, Eukaryotic Cells, hoffmann, Humans, imler, Immunity, M3i, Mutation, Viral, Virus Diseases, viruses},
pubstate = {published},
tppubtype = {article}
}
2006
Flacher Vincent, Bouschbacher Marielle, Verronèse Estelle, Massacrier Catherine, Sisirak Vanja, Berthier-Vergnes Odile, de Saint-Vis Blandine, Caux Christophe, Dezutter-Dambuyant Colette, Lebecque Serge, Valladeau Jenny
Human Langerhans cells express a specific TLR profile and differentially respond to viruses and Gram-positive bacteria Article de journal
Dans: Journal of Immunology (Baltimore, Md.: 1950), vol. 177, no. 11, p. 7959–7967, 2006, ISSN: 0022-1767.
Résumé | Liens | BibTeX | Étiquettes: bacteria, Double-Stranded, Gram-Positive Bacteria, Human, Humans, Interleukin-6, Interleukin-8, Langerhans Cells, Reverse Transcriptase Polymerase Chain Reaction, RNA, Skin, Team-Mueller, TLR4, TLR7, Toll-Like Receptors, Tumor Necrosis Factor-alpha, viruses
@article{flacher_human_2006,
title = {Human Langerhans cells express a specific TLR profile and differentially respond to viruses and Gram-positive bacteria},
author = {Vincent Flacher and Marielle Bouschbacher and Estelle Verronèse and Catherine Massacrier and Vanja Sisirak and Odile Berthier-Vergnes and Blandine de Saint-Vis and Christophe Caux and Colette Dezutter-Dambuyant and Serge Lebecque and Jenny Valladeau},
doi = {10.4049/jimmunol.177.11.7959},
issn = {0022-1767},
year = {2006},
date = {2006-12-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {177},
number = {11},
pages = {7959--7967},
abstract = {Dendritic cells (DC) are APCs essential for the development of primary immune responses. In pluristratified epithelia, Langerhans cells (LC) are a critical subset of DC which take up Ags and migrate toward lymph nodes upon inflammatory stimuli. TLR allow detection of pathogen-associated molecular patterns (PAMP) by different DC subsets. The repertoire of TLR expressed by human LC is uncharacterized and their ability to directly respond to PAMP has not been systematically investigated. In this study, we show for the first time that freshly purified LC from human skin express mRNA encoding TLR1, TLR2, TLR3, TLR5, TLR6 and TLR10. In addition, keratinocytes ex vivo display TLR1-5, TLR7, and TLR10. Accordingly, highly enriched immature LC efficiently respond to TLR2 agonists peptidoglycan and lipoteichoic acid from Gram-positive bacteria, and to dsRNA which engages TLR3. In contrast, LC do not directly sense TLR7/8 ligands and LPS from Gram-negative bacteria, which signals through TLR4. TLR engagement also results in cytokine production, with marked differences depending on the PAMP detected. TLR2 and TLR3 ligands increase IL-6 and IL-8 production, while dsRNA alone stimulates TNF-alpha release. Strikingly, only peptidoglycan triggers IL-10 secretion, thereby suggesting a specific function in tolerance to commensal Gram-positive bacteria. However, LC do not produce IL-12p70 or type I IFNs. In conclusion, human LC are equipped with TLR that enable direct detection of PAMP from viruses and Gram-positive bacteria, subsequent phenotypic maturation, and differential cytokine production. This implies a significant role for LC in the control of skin immune responses.},
keywords = {bacteria, Double-Stranded, Gram-Positive Bacteria, Human, Humans, Interleukin-6, Interleukin-8, Langerhans Cells, Reverse Transcriptase Polymerase Chain Reaction, RNA, Skin, Team-Mueller, TLR4, TLR7, Toll-Like Receptors, Tumor Necrosis Factor-alpha, viruses},
pubstate = {published},
tppubtype = {article}
}