Publications
2011
Semaan N, Frenzel L, Alsaleh G, Suffert G, Gottenberg J E, Sibilia J, Pfeffer S, Wachsmann D
miR-346 controls release of TNF-alpha protein and stability of its mRNA in rheumatoid arthritis via tristetraprolin stabilization Article de journal
Dans: PLoS One, vol. 6, no. 5, p. e19827, 2011, ISSN: 1932-6203 (Electronic) 1932-6203 (Linking), (Semaan, Noha Frenzel, Laurent Alsaleh, Ghada Suffert, Guillaume Gottenberg, Jacques-Eric Sibilia, Jean Pfeffer, Sebastien Wachsmann, Dominique Research Support, Non-U.S. Gov't United States PloS one PLoS One. 2011;6(5):e19827. Epub 2011 May 17. DOI: 10.1371/journal.pone.0019827).
Résumé | Liens | BibTeX | Étiquettes: Antisense/metabolism RNA, arthritis, Biological Protein Stability/drug effects Protein-Tyrosine Kinases/metabolism *RNA Stability/drug effects RNA, Messenger/genetics/metabolism Reverse Transcriptase Polymerase Chain Reaction Synovial Fluid/cytology Transfection Tristetraprolin/*metabolism Tumor Necrosis Factor-alpha/biosynthesis/genetics/*secretion, PFEFFER, Rheumatoid/*genetics/pathology Cell Line Fibroblasts/drug effects/metabolism/pathology Gene Expression Regulation Humans Lipopolysaccharides/pharmacology MicroRNAs/genetics/*metabolism Models, Unité ARN
@article{,
title = {miR-346 controls release of TNF-alpha protein and stability of its mRNA in rheumatoid arthritis via tristetraprolin stabilization},
author = {N Semaan and L Frenzel and G Alsaleh and G Suffert and J E Gottenberg and J Sibilia and S Pfeffer and D Wachsmann},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21611196},
doi = {10.1371/journal.pone.0019827},
issn = {1932-6203 (Electronic) 1932-6203 (Linking)},
year = {2011},
date = {2011-01-01},
journal = {PLoS One},
volume = {6},
number = {5},
pages = {e19827},
abstract = {TNF-alpha is a major cytokine implicated in rheumatoid arthritis. Its expression is regulated both at the transcriptional and posttranscriptional levels and recent data demonstrated that miRNAs are implicated in TNF-alpha response in macrophages. LPS-activated FLS isolated from RA patients express TNF-alpha mRNA but not the mature protein. This prompted us to look for miRNAs which could be implicated in this anti-inflammatory effect. Using a microarray, we found two miRNAs, miR-125b and miR-939 predicted to target the 3'-UTR of TNF-alpha mRNA, to be up-regulated in RA FLS in response to LPS, but their repression did not restore mature TNF-alpha expression in FLS. We showed previously that miR-346, which is upregulated in LPS-activated FLS, inhibited Btk expression that stabilized TNF-alpha mRNA. Blocking miR-346 reestablished TNF-alpha expression in activated FLS. Interestingly, transfection of miR-346 in LPS-activated THP-1 cells inhibited TNF-alpha secretion. We also demonstrated that TTP, a RNA binding protein which inhibited TNF-alpha synthesis, is overexpressed in activated FLS and that inhibition of miR-346 decreases its expression. Conversely, transfection of miR-346 in LPS-activated THP-1 cells increased TTP mRNA expression and inhibited TNF-alpha release. These results indicate that miR-346 controls TNF-alpha synthesis by regulating TTP expression.},
note = {Semaan, Noha
Frenzel, Laurent
Alsaleh, Ghada
Suffert, Guillaume
Gottenberg, Jacques-Eric
Sibilia, Jean
Pfeffer, Sebastien
Wachsmann, Dominique
Research Support, Non-U.S. Gov't
United States
PloS one
PLoS One. 2011;6(5):e19827. Epub 2011 May 17.
DOI: 10.1371/journal.pone.0019827},
keywords = {Antisense/metabolism RNA, arthritis, Biological Protein Stability/drug effects Protein-Tyrosine Kinases/metabolism *RNA Stability/drug effects RNA, Messenger/genetics/metabolism Reverse Transcriptase Polymerase Chain Reaction Synovial Fluid/cytology Transfection Tristetraprolin/*metabolism Tumor Necrosis Factor-alpha/biosynthesis/genetics/*secretion, PFEFFER, Rheumatoid/*genetics/pathology Cell Line Fibroblasts/drug effects/metabolism/pathology Gene Expression Regulation Humans Lipopolysaccharides/pharmacology MicroRNAs/genetics/*metabolism Models, Unité ARN},
pubstate = {published},
tppubtype = {article}
}