Biologie Digitale de l’ARN

@article{,

title = {The use of chemical modification interference and inverse PCR mutagenesis to identify the dimerization initiation site of HIV-1 genomic RNA},

author = {J C Paillart and E Skripkin and B Ehresmann and C Ehresmann and R Marquet},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8786995},

isbn = {8786995},

year  = {1996},

date = {1996-01-01},

journal = {Pharm Acta Helv},

volume = {71},

number = {1},

pages = {21-28},

abstract = {The retroviral genome consists of two identical RNA molecules physically linked together close to their 5' end, in a region called the Dimer Linkage Structure (DLS). Recent findings suggest that dimerization is involved in encapsidation, regulation of translation and reverse transcription. Previous in vitro studies localized the DLS of HIV-1 in a region downstream of the splice donor (SD) site. More recently, we showed that dimerization of HIV-1 RNA also involves sequences upstream of the SD site. Modification interference experiments and site-directed mutagenesis were used to identify the nucleotides required in the dimerization process of HIV-1 RNA. Our results point out a self-complementary sequence located in a hairpin loop, between the Primer Binding Site (PBS) and the SD site, as the Dimerization Initiation Site.},

note = {0031-6865 

Journal Article},

keywords = {Base Sequence  *Genome, MARQUET, Non-U.S. Gov't, PAILLART, Site-Directed  Polymerase Chain Reaction  RNA, Unité ARN, Viral  HIV-1/*chemistry  Human  Molecular Sequence Data  Mutagenesis, Viral/*chemistry  Support},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {The use of chemical modification interference and inverse PCR mutagenesis to identify the dimerization initiation site of HIV-1 genomic RNA},

author = {J C Paillart and E Skripkin and B Ehresmann and C Ehresmann and R Marquet},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8786995},

isbn = {8786995},

year  = {1996},

date = {1996-01-01},

journal = {Pharm Acta Helv},

volume = {71},

number = {1},

pages = {21-28},

abstract = {The retroviral genome consists of two identical RNA molecules physically linked together close to their 5' end, in a region called the Dimer Linkage Structure (DLS). Recent findings suggest that dimerization is involved in encapsidation, regulation of translation and reverse transcription. Previous in vitro studies localized the DLS of HIV-1 in a region downstream of the splice donor (SD) site. More recently, we showed that dimerization of HIV-1 RNA also involves sequences upstream of the SD site. Modification interference experiments and site-directed mutagenesis were used to identify the nucleotides required in the dimerization process of HIV-1 RNA. Our results point out a self-complementary sequence located in a hairpin loop, between the Primer Binding Site (PBS) and the SD site, as the Dimerization Initiation Site.},

note = {0031-6865 

Journal Article},

keywords = {Base Sequence  *Genome, MARQUET, Non-U.S. Gov't, PAILLART, Site-Directed  Polymerase Chain Reaction  RNA, Unité ARN, Viral  HIV-1/*chemistry  Human  Molecular Sequence Data  Mutagenesis, Viral/*chemistry  Support},

pubstate = {published},

tppubtype = {article}

}