Publications
2000
Moine H, Squires C L, Ehresmann B, Ehresmann C
In vivo selection of functional ribosomes with variations in the rRNA-binding site of Escherichia coli ribosomal protein S8: evolutionary implications Article de journal
Dans: Proc Natl Acad Sci U S A, vol. 97, no. 2, p. 605-610, 2000, ISBN: 10639126, (0027-8424http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10639126 Journal Article).
Résumé | Liens | BibTeX | Étiquettes: 16S/genetics/metabolism Recombinant Fusion Proteins/genetics/metabolism Ribosomal Proteins/genetics/*metabolism Ribosomes/genetics/*metabolism Spectinomycin/pharmacology Support, Base Sequence Binding Sites/genetics Binding, Competitive Cell Division/genetics Cloning, Microbial Escherichia coli/drug effects/*genetics/metabolism Evolution, Molecular Drug Resistance, Molecular Protein Binding RNA, P.H.S. Variation (Genetics), Ribosomal, Ribosomal/genetics/*metabolism RNA, U.S. Gov't, Unité ARN
@article{,
title = {In vivo selection of functional ribosomes with variations in the rRNA-binding site of Escherichia coli ribosomal protein S8: evolutionary implications},
author = {H Moine and C L Squires and B Ehresmann and C Ehresmann},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10639126},
isbn = {10639126},
year = {2000},
date = {2000-01-01},
journal = {Proc Natl Acad Sci U S A},
volume = {97},
number = {2},
pages = {605-610},
abstract = {The highly conserved nature of rRNA sequences throughout evolution allows these molecules to be used to build philogenic trees of different species. It is unknown whether the stability of specific interactions and structural features of rRNA reflects an optimal adaptation to a functional task or an evolutionary trap. In the work reported here, we have applied an in vivo selection strategy to demonstrate that unnatural sequences do work as a functional replacement of the highly conserved binding site of ribosomal protein S8. However, growth competition experiments performed between Escherichia coli isolates containing natural and unnatural S8-binding sites showed that the fate of each isolate depended on the growth condition. In exponentially growing cells, one unnatural variant was found to be equivalent to wild type in competition experiments performed in rich media. In culture conditions leading to slow growth, however, cells containing the wild-type sequence were the ultimate winner of the competition, emphasizing that the wild-type sequence is, in fact, the most fit solution for the S8-binding site.},
note = {0027-8424http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10639126
Journal Article},
keywords = {16S/genetics/metabolism Recombinant Fusion Proteins/genetics/metabolism Ribosomal Proteins/genetics/*metabolism Ribosomes/genetics/*metabolism Spectinomycin/pharmacology Support, Base Sequence Binding Sites/genetics Binding, Competitive Cell Division/genetics Cloning, Microbial Escherichia coli/drug effects/*genetics/metabolism Evolution, Molecular Drug Resistance, Molecular Protein Binding RNA, P.H.S. Variation (Genetics), Ribosomal, Ribosomal/genetics/*metabolism RNA, U.S. Gov't, Unité ARN},
pubstate = {published},
tppubtype = {article}
}