Biologie Digitale de l’ARN

@article{,

title = {Dimerization of human immunodeficiency virus type 1 RNA involves sequences located upstream of the splice donor site},

author = {R Marquet and J C Paillart and E Skripkin and C Ehresmann and B Ehresmann},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8121797},

isbn = {8121797},

year  = {1994},

date = {1994-01-01},

journal = {Nucleic Acids Res},

volume = {22},

number = {2},

pages = {145-151},

abstract = {The retroviral genome consists of two homologous RNA molecules associated close to their 5' ends. We studied the spontaneous dimerization of four HIV-1 RNA fragments (RNAs 1-707, 1-615, 311-612, and 311-415) containing the previously defined dimerization domain, and a RNA fragment (RNA 1-311) corresponding to the upstream sequences. Significant dimerization of all RNAs is observed on agarose gels when magnesium is included in the electrophoresis buffer. In contrast to dimerization of RNAs 311-612 and 311-415, dimerization of RNAs 1-707, 1-615 and 1-311 strongly depends on the size of the monovalent cation present in the incubation buffer. Also, dimerization of RNAs 1-707, 1-615, and 1-311 is 10 times faster than that of RNAs 311-612 and 311-415. The dimers formed by the latter RNAs are substantially more stable than that of RNA 1-615, while RNA 1-311 dimer is 5-7 degrees C less stable than RNA 1-615 dimer. These results indicate that dimerization of HIV-1 genomic RNA involves elements located upstream of the splice donor site (position 305), i.e. outside of the previously defined dimerization domain.},

note = {0305-1048 

Journal Article},

keywords = {Base Sequence  Cations  HIV-1/*genetics  Kinetics  Macromolecular Systems  Magnesium  Models, Chemical  Models, Genetic  Molecular Sequence Data  RNA Splicing  RNA, MARQUET, Non-U.S. Gov't  Thermodynamics, PAILLART, Unité ARN, Viral/*chemistry  Support},

pubstate = {published},

tppubtype = {article}

}