Publications
2004
Costa A, de Barros J P Pais, Keith G, Baranowski W, Desgres J
Determination of queuosine derivatives by reverse-phase liquid chromatography for the hypomodification study of Q-bearing tRNAs from various mammal liver cells Article de journal
Dans: J Chromatogr B Analyt Technol Biomed Life Sci, vol. 801, no. 2, p. 237-247, 2004, ISBN: 14751792, (1570-0232 Journal Article).
Résumé | Liens | BibTeX | Étiquettes: Amino Acyl/chemistry RNA, Asn/chemistry Rats Support, Cultured, Cultured Chickens *Chromatography, Experimental Nucleoside Q/*analogs & derivatives/*analysis RNA, High Pressure Liquid Hepatocytes/chemistry Liver/*chemistry Liver Neoplasms, KEITH Animals Cells, Non-U.S. Gov't Tumor Cells, Transfer, Transfer/*chemistry/isolation & purification RNA, Unité ARN
@article{,
title = {Determination of queuosine derivatives by reverse-phase liquid chromatography for the hypomodification study of Q-bearing tRNAs from various mammal liver cells},
author = {A Costa and J P Pais de Barros and G Keith and W Baranowski and J Desgres},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14751792},
isbn = {14751792},
year = {2004},
date = {2004-01-01},
journal = {J Chromatogr B Analyt Technol Biomed Life Sci},
volume = {801},
number = {2},
pages = {237-247},
abstract = {Three queuosine derivatives (Q-derivatives) have been found at position 34 of four mammalian so-called Q-tRNAs: queuosine (Q) in tRNA(Asn) and tRNA(His), mannosyl-queuosine (manQ) in tRNA(Asp), and galactosyl-queuosine (galQ) in tRNA(Tyr). An analytical procedure based on the combined means of purified tRNA isolation from liver cells and ribonucleoside analysis by reverse-phase high performance liquid chromatography coupled with real-time UV-spectrometry (RPLC-UV) was developed for the quantitative analysis of the three Q-derivatives present in total tRNA from liver tissues and liver cell cultures. Using this analytical procedure, the rates of Q-tRNA modification were studied in total tRNAs from various mammalian hepatic cells. Our results show that the four Q-tRNAs are fully modified in liver tissues from adult mammals, regardless of the mammal species. However, a lack in the Q-modification level was observed in Q-tRNAs from newborn rat liver, as well in Q-tRNAs from normal rat liver cell cultures growing in a low queuine content medium, and from a rat hepatoma cell line. It is noteworthy that in all cases of Q-tRNA hypomodification, our analytical procedure showed that tRNA(Asp) is always the least affected by the hypomodification. The biological significance of this phenomenon is discussed.},
note = {1570-0232
Journal Article},
keywords = {Amino Acyl/chemistry RNA, Asn/chemistry Rats Support, Cultured, Cultured Chickens *Chromatography, Experimental Nucleoside Q/*analogs & derivatives/*analysis RNA, High Pressure Liquid Hepatocytes/chemistry Liver/*chemistry Liver Neoplasms, KEITH Animals Cells, Non-U.S. Gov't Tumor Cells, Transfer, Transfer/*chemistry/isolation & purification RNA, Unité ARN},
pubstate = {published},
tppubtype = {article}
}