Biologie Digitale de l’ARN

@article{,

title = {Variations in tRNA modifications, particularly of their queuine content in higher eukaryotes. Its relation to malignancy grading},

author = { G. Dirheimer and W. Baranowski and G. Keith},

year  = {1995},

date = {1995-01-01},

journal = {Biochimie},

volume = {77},

number = {1-2},

pages = {99-103},

abstract = {Literature references dealing with the variations in the modification level of nucleosides in total eukaryotic tRNAs as a function of different physiological status and after drug administration as well as in sequenced cytoplasmic tRNAs between normal and tumor cells and in SV40-transformed cells are reviewed. In addition, special attention is given to guanine replacement of queuine in the first position of the anticodon of tRNAs. A correlation between the level of this undermodification in cancer tissues and the malignancy grading could be found in human ovarian tumors, confirming the results reported in several laboratories for lymphomas and lung cancer tissues. Indeed tRNAs from primary and metastatic human ovarian malignant tumors are Q deficient as compared to tRNAs from normal tissues or benign tumors: thus queuine deficiency increases with malignancy and grading of differentiation.},

note = {0300-9084 

Journal Article 

Review 

Review, Tutorial},

keywords = {&, Animals, Cell, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Neoplasms/*genetics/pathology, Neoplastic/genetics, Non-U.S., Ovarian, post-transcriptional, Processing, Purines/analysis, Pyrimidines/analysis, RNA, Support, Transfer/*chemistry/metabolism, Transformation},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Deficiency of queuine, a highly modified purine base, in transfer RNAs from primary and metastatic ovarian malignant tumors in women},

author = { W. Baranowski and G. Dirheimer and J. A. Jakowicki and G. Keith},

year  = {1994},

date = {1994-01-01},

journal = {Cancer Res},

volume = {54},

number = {16},

pages = {4468-71},

abstract = {The tRNAs from rapidly growing tissues, particularly from neoplasia, often exhibit queuine deficiency. In order to check whether different kinds of ovarian tumors display queuine deficiencies we have analyzed tRNA samples from 16 ovarian malignancies. The tRNAs from histologically normal myometrium (4 samples) and myoma (6 samples) were taken as healthy tissue and benign tumor references. Queuine deficiency was determined by an exchange assay using [8-3H]guanine and tRNA:guanine transglycosylase from Escherichia coli. The mean values of queuine deficiencies in tRNAs were: 10.95 +/- 2.21 (SD) pmol/A260 in gonadal and germ cell tumors (5 cases); 23.75 +/- 7.89 pmol/A260 in primary epithelial tumors (9 cases); and 34.58 +/- 7.18 pmol/A260 in metastatic tumors (2 cases). These values displayed statistically significant differences (P = 0.0003, Kruskal-Wallis test). The queuine deficiencies in tRNAs significantly increased when moving from well-differentiated through moderately differentiated to poorly differentiated tumors, with the highest values found in poorly differentiated metastatic tumors (P = 0.0002, Kruskal-Wallis test). Queuine deficiency determination in tRNAs is proposed as a factor for clinical outcome prognosis of ovarian malignancies.},

note = {0008-5472 

Journal Article},

keywords = {&, Adolescent, Adult, Aged, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Middle, Neoplasm/*chemistry, Neoplasms/*chemistry/pathology, Non-U.S., Ovarian, RNA, Support, Transfer/*chemistry},

pubstate = {published},

tppubtype = {article}

}