Publications
2007
Bernacchi S, Henriet S, Dumas P, Paillart J C, Marquet R
RNA and DNA binding properties of HIV-1 Vif protein: a fluorescence study Article de journal
Dans: J Biol Chem, vol. 282, no. 36, p. 26361-26368, 2007, ISBN: 17609216.
Résumé | Liens | BibTeX | Étiquettes: 5' Untranslated Regions/genetics/immunology/metabolism Binding Sites/physiology Cytidine Deaminase Cytosine Deaminase/immunology/metabolism DNA, gag/genetics/immunology/metabolism Gene Products, Human Immunodeficiency Virus, MARQUET, Natural/physiology Nucleoside Deaminases/immunology/metabolism Oligonucleotides/genetics/immunology/metabolism Protein Binding/physiology Protein Biosynthesis/physiology RNA, PAILLART, Unité ARN, vif/genetics/immunology/*metabolism Genome, Viral/genetics/immunology/*metabolism DNA-Binding Proteins/genetics/immunology/*metabolism Gene Products, Viral/genetics/immunology/*metabolism RNA-Binding Proteins/genetics/immunology/*metabolism Repressor Proteins/genetics/immunology/*metabolism vif Gene Products, Viral/physiology HIV Long Terminal Repeat/physiology HIV-1/genetics/immunology/*metabolism/pathogenicity Humans Immunity
@article{,
title = {RNA and DNA binding properties of HIV-1 Vif protein: a fluorescence study},
author = {S Bernacchi and S Henriet and P Dumas and J C Paillart and R Marquet},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17609216},
isbn = {17609216},
year = {2007},
date = {2007-01-01},
journal = {J Biol Chem},
volume = {282},
number = {36},
pages = {26361-26368},
abstract = {The HIV-1 viral infectivity factor (Vif) is a small basic protein essential for viral fitness and pathogenicity. Some "non-permissive" cell lines cannot sustain replication of Vif(-) HIV-1 virions. In these cells, Vif counteracts the natural antiretroviral activity of the DNA-editing enzymes APOBEC3G/3F. Moreover, Vif is packaged into viral particles through a strong interaction with genomic RNA in viral nucleoprotein complexes. To gain insights into determinants of this binding process, we performed the first characterization of Vif/nucleic acid interactions using Vif intrinsic fluorescence. We determined the affinity of Vif for RNA fragments corresponding to various regions of the HIV-1 genome. Our results demonstrated preferential and moderately cooperative binding for RNAs corresponding to the 5'-untranslated region of HIV-1 (5'-untranslated region) and gag (cooperativity parameter omega approximately 65-80, and K(d) = 45-55 nM). In addition, fluorescence spectroscopy allowed us to point out the TAR apical loop and a short region in gag as primary strong affinity binding sites (K(d) = 9.5-14 nM). Interestingly, beside its RNA binding properties, the Vif protein can also bind the corresponding DNA oligonucleotides and their complementary counterparts with an affinity similar to the one observed for the RNA sequences, while other DNA sequences displayed reduced affinity. Taken together, our results suggest that Vif binding to RNA and DNA offers several non-exclusive ways to counteract APOBEC3G/3F factors, in addition to the well documented Vif-induced degradation by the proteasome and to the Vif-mediated repression of translation of these antiviral factors.},
keywords = {5' Untranslated Regions/genetics/immunology/metabolism Binding Sites/physiology Cytidine Deaminase Cytosine Deaminase/immunology/metabolism DNA, gag/genetics/immunology/metabolism Gene Products, Human Immunodeficiency Virus, MARQUET, Natural/physiology Nucleoside Deaminases/immunology/metabolism Oligonucleotides/genetics/immunology/metabolism Protein Binding/physiology Protein Biosynthesis/physiology RNA, PAILLART, Unité ARN, vif/genetics/immunology/*metabolism Genome, Viral/genetics/immunology/*metabolism DNA-Binding Proteins/genetics/immunology/*metabolism Gene Products, Viral/genetics/immunology/*metabolism RNA-Binding Proteins/genetics/immunology/*metabolism Repressor Proteins/genetics/immunology/*metabolism vif Gene Products, Viral/physiology HIV Long Terminal Repeat/physiology HIV-1/genetics/immunology/*metabolism/pathogenicity Humans Immunity},
pubstate = {published},
tppubtype = {article}
}