Publications
2011
Serag Maged F, Kaji Noritada, Venturelli Enrica, Okamoto Yukihiro, Terasaka Kazuyoshi, Tokeshi Manabu, Mizukami Hajime, Braeckmans Kevin, Bianco Alberto, Baba Yoshinobu
Functional platform for controlled subcellular distribution of carbon nanotubes Article de journal
Dans: ACS nano, vol. 5, no. 11, p. 9264–9270, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Catharanthus, Exocytosis, Fluorescence Recovery After Photobleaching, Fluorescent Dyes, I2CT, Intracellular Space, Nanotubes, Surface Properties, Team-Bianco, Vacuoles
@article{serag_functional_2011,
title = {Functional platform for controlled subcellular distribution of carbon nanotubes},
author = {Maged F Serag and Noritada Kaji and Enrica Venturelli and Yukihiro Okamoto and Kazuyoshi Terasaka and Manabu Tokeshi and Hajime Mizukami and Kevin Braeckmans and Alberto Bianco and Yoshinobu Baba},
doi = {10.1021/nn2035654},
issn = {1936-086X},
year = {2011},
date = {2011-11-01},
journal = {ACS nano},
volume = {5},
number = {11},
pages = {9264--9270},
abstract = {As nanoparticles can cross different cellular barriers and access different tissues, control of their uptake and cellular fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show that the trafficking of single-walled carbon nanotubes (SWCNTs) through various subcellular membranes of the plant cell is facilitated or inhibited by attaching a suitable functional tag and controlling medium components. This enables a unique control over the uptake and the subcellular distribution of SWCNTs and provides a key strategy to promote their cellular elimination to minimize toxicity. Our results also demonstrate that SWCNTs are involved in a carrier-mediated transport (CMT) inside cells; this is a phenomenon that scientists could use to obtain novel molecular insights into CMT, with the potential translation to advances in subcellular nanobiology.},
keywords = {Biological Transport, carbon, Catharanthus, Exocytosis, Fluorescence Recovery After Photobleaching, Fluorescent Dyes, I2CT, Intracellular Space, Nanotubes, Surface Properties, Team-Bianco, Vacuoles},
pubstate = {published},
tppubtype = {article}
}
2000
Hetru Charles, Letellier L, Oren Z, Hoffmann Jules A, Shai Y
Androctonin, a hydrophilic disulphide-bridged non-haemolytic anti-microbial peptide: a plausible mode of action Article de journal
Dans: Biochem. J., vol. 345 Pt 3, p. 653–664, 2000, ISSN: 0264-6021.
Résumé | BibTeX | Étiquettes: Adenosine Triphosphate, Anti-Bacterial Agents, Cations, Cell Membrane Permeability, Cytoplasm, Disulfides, Electron, Escherichia coli, Fluoresceins, Fluorescent Dyes, Fourier Transform Infrared, Gram-Negative Bacteria, hoffmann, Insect Proteins, Liposomes, M3i, Microbial Sensitivity Tests, Micrococcus luteus, Microscopy, oxygen, Phospholipids, Potassium, Proteins, spectroscopy
@article{hetru_androctonin_2000,
title = {Androctonin, a hydrophilic disulphide-bridged non-haemolytic anti-microbial peptide: a plausible mode of action},
author = {Charles Hetru and L Letellier and Z Oren and Jules A Hoffmann and Y Shai},
issn = {0264-6021},
year = {2000},
date = {2000-01-01},
journal = {Biochem. J.},
volume = {345 Pt 3},
pages = {653--664},
abstract = {Androctonin is a 25-residue non-haemolytic anti-microbial peptide isolated from the scorpion Androctonus australis and contains two disulphide bridges. Androctonin is different from known native anti-microbial peptides, being a relatively hydrophilic and non-amphipathic molecule. This raises the possibility that the target of androctonin might not be the bacterial membrane, shown to be a target for most amphipathic lytic peptides. To shed light on its mode of action on bacteria and its non-haemolytic activity, we synthesized androctonin, its fluorescent derivatives and its all-D-amino acid enantiomer. The enantiomer preserved high activity, suggesting a lipid-peptide interaction between androctonin and bacterial membranes. In Gram-positive and (at higher concentrations) Gram-negative bacteria, androctonin induced an immediate perturbation of the permeability properties of the cytoplasmic membrane of the bacterial energetic state, concomitant with perturbation of the morphology of the cell envelope as revealed by electron microscopy. Androctonin binds only to negatively charged lipid vesicles and induces the leakage of markers at high concentrations and with a slow kinetics, in contrast with amphipathic alpha-helical anti-microbial peptides that bind and permeate negatively charged vesicles, and to a smaller extent also zwitterionic ones. This might explain the selective lytic activity of androctonin towards bacteria but not red blood cells. Polarized attenuated total reflection-Fourier transform infrared spectroscopy revealed that androctonin adopts a beta-sheet structure in membranes and did not affect the lipid acyl chain order, which supports a detergent-like effect. The small size of androctonin, its hydrophilic character and its physicochemical properties are favourable features for its potential application as a replacement for commercially available antibiotics to which bacteria have developed resistance.},
keywords = {Adenosine Triphosphate, Anti-Bacterial Agents, Cations, Cell Membrane Permeability, Cytoplasm, Disulfides, Electron, Escherichia coli, Fluoresceins, Fluorescent Dyes, Fourier Transform Infrared, Gram-Negative Bacteria, hoffmann, Insect Proteins, Liposomes, M3i, Microbial Sensitivity Tests, Micrococcus luteus, Microscopy, oxygen, Phospholipids, Potassium, Proteins, spectroscopy},
pubstate = {published},
tppubtype = {article}
}