Publications
2014
Schwarzmüller Tobias, Ma Biao, Hiller Ekkehard, Istel Fabian, Tscherner Michael, Brunke Sascha, Ames Lauren, Firon Arnaud, Green Brian, Cabral Vitor, Marcet-Houben Marina, Jacobsen Ilse D, Quintin Jessica, Seider Katja, Frohner Ingrid, Glaser Walter, Jungwirth Helmut, Bachellier-Bassi Sophie, Chauvel Murielle, Zeidler Ute, Ferrandon Dominique, Gabaldón Toni, Hube Bernhard, d'Enfert Christophe, Rupp Steffen, Cormack Brendan, Haynes Ken, Kuchler Karl
Systematic phenotyping of a large-scale Candida glabrata deletion collection reveals novel antifungal tolerance genes Article de journal
Dans: PLoS Pathog., vol. 10, no. 6, p. e1004211, 2014, ISSN: 1553-7374.
Résumé | Liens | BibTeX | Étiquettes: Antifungal Agents, Azoles, Biofilms, Candida glabrata, Candidiasis, Cell Wall, Drug Resistance, Echinocandins, ferrandon, Fungal, Fungal Proteins, Gene Deletion, Gene Knockout Techniques, Gene Library, M3i, Microbial Sensitivity Tests, Osmotic Pressure, Phenotype
@article{schwarzmuller_systematic_2014b,
title = {Systematic phenotyping of a large-scale Candida glabrata deletion collection reveals novel antifungal tolerance genes},
author = {Tobias Schwarzmüller and Biao Ma and Ekkehard Hiller and Fabian Istel and Michael Tscherner and Sascha Brunke and Lauren Ames and Arnaud Firon and Brian Green and Vitor Cabral and Marina Marcet-Houben and Ilse D Jacobsen and Jessica Quintin and Katja Seider and Ingrid Frohner and Walter Glaser and Helmut Jungwirth and Sophie Bachellier-Bassi and Murielle Chauvel and Ute Zeidler and Dominique Ferrandon and Toni Gabaldón and Bernhard Hube and Christophe d'Enfert and Steffen Rupp and Brendan Cormack and Ken Haynes and Karl Kuchler},
doi = {10.1371/journal.ppat.1004211},
issn = {1553-7374},
year = {2014},
date = {2014-01-01},
journal = {PLoS Pathog.},
volume = {10},
number = {6},
pages = {e1004211},
abstract = {The opportunistic fungal pathogen Candida glabrata is a frequent cause of candidiasis, causing infections ranging from superficial to life-threatening disseminated disease. The inherent tolerance of C. glabrata to azole drugs makes this pathogen a serious clinical threat. To identify novel genes implicated in antifungal drug tolerance, we have constructed a large-scale C. glabrata deletion library consisting of 619 unique, individually bar-coded mutant strains, each lacking one specific gene, all together representing almost 12% of the genome. Functional analysis of this library in a series of phenotypic and fitness assays identified numerous genes required for growth of C. glabrata under normal or specific stress conditions, as well as a number of novel genes involved in tolerance to clinically important antifungal drugs such as azoles and echinocandins. We identified 38 deletion strains displaying strongly increased susceptibility to caspofungin, 28 of which encoding proteins that have not previously been linked to echinocandin tolerance. Our results demonstrate the potential of the C. glabrata mutant collection as a valuable resource in functional genomics studies of this important fungal pathogen of humans, and to facilitate the identification of putative novel antifungal drug target and virulence genes.},
keywords = {Antifungal Agents, Azoles, Biofilms, Candida glabrata, Candidiasis, Cell Wall, Drug Resistance, Echinocandins, ferrandon, Fungal, Fungal Proteins, Gene Deletion, Gene Knockout Techniques, Gene Library, M3i, Microbial Sensitivity Tests, Osmotic Pressure, Phenotype},
pubstate = {published},
tppubtype = {article}
}
1999
Otten L, Salomone J Y, Helfer A, Schmidt J, Hammann P, Ruffray P De
Sequence and functional analysis of the left-hand part of the Ŧ-region from the nopaline-type Ti plasmid, pTiC58. Article de journal
Dans: Plant molecular biology, vol. 41, no. 6, p. 765–776, 1999, ISSN: 0167-4412 0167-4412, (Place: Netherlands).
Résumé | Liens | BibTeX | Étiquettes: Agrobacterium tumefaciens/*genetics/pathogenicity, Bacterial/chemistry/*genetics, Bacterial/genetics, Chromosome Mapping, DNA, Gene Deletion, Genes, Genetic Complementation Test, Lycopersicon esculentum/genetics/microbiology, Medicinal/genetics/microbiology, Molecular Sequence Data, Mutation, Phylogeny, Plant Tumors/genetics/microbiology, Plants, Plasmids/chemistry/*genetics, PPSE, Sequence Analysis, Species Specificity, Tobacco/genetics/microbiology, Toxic, Virulence/genetics
@article{otten_sequence_1999,
title = {Sequence and functional analysis of the left-hand part of the Ŧ-region from the nopaline-type Ti plasmid, pTiC58.},
author = {L Otten and J Y Salomone and A Helfer and J Schmidt and P Hammann and P De Ruffray},
doi = {10.1023/a:1006370207379},
issn = {0167-4412 0167-4412},
year = {1999},
date = {1999-12-01},
journal = {Plant molecular biology},
volume = {41},
number = {6},
pages = {765--776},
abstract = {The Agrobacterium tumefaciens nopaline strain C58 transfers a large, 29 kb T-DNA into plant cells during infection. Part of this DNA (the 'common DNA') is also found on the T-DNA of octopine strains, the remaining DNA is nopaline strain-specific. Up to now, only parts of the C58 T-DNA and related T37 T-DNA have been sequenced. We have sequenced the remainder of the nopaline-specific T-DNA (containing genes a to d) and acs to iaaM. Gene c codes for a new unknown T-DNA protein. Gene a is homologous to the agrocinopine synthase gene. Genes b, c', d and e are part of a larger family: they are related to the T-DNA genes 5, rolB, lso and 3'. Genes 5, rolB and lso induce or modify plant growth and have been called T-DNA oncogenes. Our studies show that gene 3' (located on the TR-DNA of octopine strains) is also oncogenic. Although the b-e T-DNA fragment from C58 and its individual genes lack growth-inducing activity, an a-acs deletion mutant was distinctly less virulent on Kalanchoe daigremontiana and showed reduced shoot formation on Kalanchoe tubiflora. Shoot formation could be restored by genes c and c' in co-infection experiments. Contrary to an earlier report, a C58 e gene deletion mutant was fully virulent on all plants tested.},
note = {Place: Netherlands},
keywords = {Agrobacterium tumefaciens/*genetics/pathogenicity, Bacterial/chemistry/*genetics, Bacterial/genetics, Chromosome Mapping, DNA, Gene Deletion, Genes, Genetic Complementation Test, Lycopersicon esculentum/genetics/microbiology, Medicinal/genetics/microbiology, Molecular Sequence Data, Mutation, Phylogeny, Plant Tumors/genetics/microbiology, Plants, Plasmids/chemistry/*genetics, PPSE, Sequence Analysis, Species Specificity, Tobacco/genetics/microbiology, Toxic, Virulence/genetics},
pubstate = {published},
tppubtype = {article}
}