Publications
2021
Leite Thiago H J F, Ferreira Alvaro G A, Imler Jean-Luc, Marques João T
Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes Article de journal
Dans: Frontiers in immunology, vol. 12, p. 660873, 2021.
Résumé | Liens | BibTeX | Étiquettes: Aedes, Dengue, Hemocytes, imler, innate immunity, M3i, Marques, Zika
@article{Leite2021,
title = {Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes},
author = {Thiago H J F Leite and Alvaro G A Ferreira and Jean-Luc Imler and João T Marques},
url = {https://www.frontiersin.org/articles/10.3389/fimmu.2021.660873/full},
doi = {10.3389/fimmu.2021.660873},
year = {2021},
date = {2021-05-13},
journal = {Frontiers in immunology},
volume = {12},
pages = {660873},
abstract = {Aedes aegypti mosquitoes are vectors for arboviruses of medical importance such as dengue (DENV) and Zika (ZIKV) viruses. Different innate immune pathways contribute to the control of arboviruses in the mosquito vector including RNA interference, Toll and Jak- STAT pathways. However, the role of cellular responses mediated by circulating macrophage-like cells known as hemocytes remains unclear. Here we show that hemocytes are recruited to the midgut of Ae. aegypti mosquitoes in response to DENV or ZIKV. Blockade of the phagocytic function of hemocytes using latex beads induced increased accumulation of hemocytes in the midgut and a reduction in virus infection levels in this organ. In contrast, inhibition of phagocytosis by hemocytes led to increased systemic dissemination and replication of DENV and ZIKV. Hence, our work reveals a dual role for hemocytes in Ae. aegypti mosquitoes, whereby phagocytosis is not required to control viral infection in the midgut but is essential to restrict systemic dissemination. Further understanding of the mechanism behind this duality could help the design of vector-based strategies to prevent transmission of arboviruses.},
keywords = {Aedes, Dengue, Hemocytes, imler, innate immunity, M3i, Marques, Zika},
pubstate = {published},
tppubtype = {article}
}
2020
Liégeois Samuel, Ferrandon Dominique
An atlas for hemocytes in an insect Article de journal
Dans: Elife, 2020.
Résumé | Liens | BibTeX | Étiquettes: Drosophila, ferrandon, Hemocytes, M3i, single-cell
@article{Liégeois2020b,
title = {An atlas for hemocytes in an insect},
author = {Samuel Liégeois and Dominique Ferrandon},
editor = {Elife},
url = {https://elifesciences.org/articles/59113},
doi = {10.7554/eLife.59113},
year = {2020},
date = {2020-06-30},
journal = {Elife},
abstract = {Single-cell RNA sequencing has revealed distinct subpopulations of hemocytes in fruit fly larvae},
keywords = {Drosophila, ferrandon, Hemocytes, M3i, single-cell},
pubstate = {published},
tppubtype = {article}
}
2016
Lamiable Olivier, Arnold Johan, da de Faria Isaque Joao Silva, Olmo Roenick Proveti, Bergami Francesco, Meignin Carine, Hoffmann Jules A, Marques Joao Trindade, Imler Jean-Luc
Analysis of the Contribution of Hemocytes and Autophagy to Drosophila Antiviral Immunity Article de journal
Dans: J. Virol., vol. 90, no. 11, p. 5415–5426, 2016, ISSN: 0022-538X, 1098-5514.
Liens | BibTeX | Étiquettes: antiviral immunity, Autophagy, Hemocytes, hoffmann, imler, M3i, meignin
@article{lamiable_analysis_2016,
title = {Analysis of the Contribution of Hemocytes and Autophagy to Drosophila Antiviral Immunity},
author = {Olivier Lamiable and Johan Arnold and Isaque Joao Silva da de Faria and Roenick Proveti Olmo and Francesco Bergami and Carine Meignin and Jules A Hoffmann and Joao Trindade Marques and Jean-Luc Imler},
url = {http://jvi.asm.org/content/90/11/5415},
doi = {10.1128/JVI.00238-16},
issn = {0022-538X, 1098-5514},
year = {2016},
date = {2016-01-01},
urldate = {2016-06-05},
journal = {J. Virol.},
volume = {90},
number = {11},
pages = {5415--5426},
keywords = {antiviral immunity, Autophagy, Hemocytes, hoffmann, imler, M3i, meignin},
pubstate = {published},
tppubtype = {article}
}
2011
Aoun Richard Bou, Hetru Charles, Troxler Laurent, Doucet Daniel, Ferrandon Dominique, Matt Nicolas
Analysis of thioester-containing proteins during the innate immune response of Drosophila melanogaster Article de journal
Dans: J Innate Immun, vol. 3, no. 1, p. 52–64, 2011, ISSN: 1662-8128.
Résumé | Liens | BibTeX | Étiquettes: Animals, bioinformatic, DNA, Evolution, ferrandon, Gene Expression Regulation, Hemocytes, Immunity, In Situ Hybridization, Innate, M3i, matt, Molecular, Mutation, Phylogeny, Sequence Analysis
@article{bou_aoun_analysis_2011,
title = {Analysis of thioester-containing proteins during the innate immune response of Drosophila melanogaster},
author = {Richard Bou Aoun and Charles Hetru and Laurent Troxler and Daniel Doucet and Dominique Ferrandon and Nicolas Matt},
doi = {10.1159/000321554},
issn = {1662-8128},
year = {2011},
date = {2011-01-01},
journal = {J Innate Immun},
volume = {3},
number = {1},
pages = {52--64},
abstract = {Thioester-containing proteins (TEPs) are conserved proteins among insects that are thought to be involved in innate immunity. In Drosophila, the Tep family is composed of 6 genes named Tep1-Tep6. In this study, we investigated the phylogeny, expression pattern and roles of these genes in the host defense of Drosophila. Protostomian Tep genes are clustered in 3 distinct branches, 1 of which is specific to mosquitoes. Most D. melanogaster Tep genes are expressed in hemocytes, can be induced in the fat body, and are expressed in specific regions of the hypodermis. This expression pattern is consistent with a role in innate immunity. However, we find that TEP1, TEP2, and TEP4 are not strictly required in the body cavity to fight several bacterial and fungal infections. One possibility is that Drosophila TEPs act redundantly or that their absence can be compensated by other components of the immune response. TEPs may thus provide a subtle selective advantage during evolution. Alternatively, they may be required in host defense against specific as yet unidentified natural pathogens of Drosophila.},
keywords = {Animals, bioinformatic, DNA, Evolution, ferrandon, Gene Expression Regulation, Hemocytes, Immunity, In Situ Hybridization, Innate, M3i, matt, Molecular, Mutation, Phylogeny, Sequence Analysis},
pubstate = {published},
tppubtype = {article}
}
2010
Garcia Alvaro Baeza, Pierce Raymond J, Gourbal Benjamin, Werkmeister Elisabeth, Colinet Dominique, Reichhart Jean-Marc, Dissous Colette, Coustau Christine
Involvement of the cytokine MIF in the snail host immune response to the parasite Schistosoma mansoni Article de journal
Dans: PLoS Pathog., vol. 6, no. 9, p. e1001115, 2010, ISSN: 1553-7374.
Résumé | Liens | BibTeX | Étiquettes: Amino Acid, Animals, Apoptosis, Biomphalaria, Blotting, Cell Proliferation, Cells, Cricetinae, Cultured, Hemocytes, Host-Parasite Interactions, Humans, Liver, M3i, Macrophage Migration-Inhibitory Factors, messenger, Oocysts, Recombinant Proteins, reichhart, Reverse Transcriptase Polymerase Chain Reaction, RNA, Schistosoma mansoni, Schistosomiasis mansoni, Sequence Homology, Small Interfering, Western
@article{baeza_garcia_involvement_2010,
title = {Involvement of the cytokine MIF in the snail host immune response to the parasite Schistosoma mansoni},
author = {Alvaro Baeza Garcia and Raymond J Pierce and Benjamin Gourbal and Elisabeth Werkmeister and Dominique Colinet and Jean-Marc Reichhart and Colette Dissous and Christine Coustau},
doi = {10.1371/journal.ppat.1001115},
issn = {1553-7374},
year = {2010},
date = {2010-01-01},
journal = {PLoS Pathog.},
volume = {6},
number = {9},
pages = {e1001115},
abstract = {We have identified and characterized a Macrophage Migration Inhibitory Factor (MIF) family member in the Lophotrochozoan invertebrate, Biomphalaria glabrata, the snail intermediate host of the human blood fluke Schistosoma mansoni. In mammals, MIF is a widely expressed pleiotropic cytokine with potent pro-inflammatory properties that controls cell functions such as gene expression, proliferation or apoptosis. Here we show that the MIF protein from B. glabrata (BgMIF) is expressed in circulating immune defense cells (hemocytes) of the snail as well as in the B. glabrata embryonic (Bge) cell line that has hemocyte-like features. Recombinant BgMIF (rBgMIF) induced cell proliferation and inhibited NO-dependent p53-mediated apoptosis in Bge cells. Moreover, knock-down of BgMIF expression in Bge cells interfered with the in vitro encapsulation of S. mansoni sporocysts. Furthermore, the in vivo knock-down of BgMIF prevented the changes in circulating hemocyte populations that occur in response to an infection by S. mansoni miracidia and led to a significant increase in the parasite burden of the snails. These results provide the first functional evidence that a MIF ortholog is involved in an invertebrate immune response towards a parasitic infection and highlight the importance of cytokines in invertebrate-parasite interactions.},
keywords = {Amino Acid, Animals, Apoptosis, Biomphalaria, Blotting, Cell Proliferation, Cells, Cricetinae, Cultured, Hemocytes, Host-Parasite Interactions, Humans, Liver, M3i, Macrophage Migration-Inhibitory Factors, messenger, Oocysts, Recombinant Proteins, reichhart, Reverse Transcriptase Polymerase Chain Reaction, RNA, Schistosoma mansoni, Schistosomiasis mansoni, Sequence Homology, Small Interfering, Western},
pubstate = {published},
tppubtype = {article}
}
2009
Shia Alice K H, Glittenberg Marcus, Thompson Gavin, Weber Alexander N R, Reichhart Jean-Marc, Ligoxygakis Petros
Toll-dependent antimicrobial responses in Drosophila larval fat body require Spätzle secreted by haemocytes Article de journal
Dans: J. Cell. Sci., vol. 122, no. Pt 24, p. 4505–4515, 2009, ISSN: 1477-9137.
Résumé | Liens | BibTeX | Étiquettes: Animals, Enterococcus faecalis, Escherichia coli, Fat Body, Hemocytes, Larva, M3i, reichhart, Toll-Like Receptors
@article{shia_toll-dependent_2009,
title = {Toll-dependent antimicrobial responses in Drosophila larval fat body require Spätzle secreted by haemocytes},
author = {Alice K H Shia and Marcus Glittenberg and Gavin Thompson and Alexander N R Weber and Jean-Marc Reichhart and Petros Ligoxygakis},
doi = {10.1242/jcs.049155},
issn = {1477-9137},
year = {2009},
date = {2009-12-01},
journal = {J. Cell. Sci.},
volume = {122},
number = {Pt 24},
pages = {4505--4515},
abstract = {In Drosophila, the humoral response characterised by the synthesis of antimicrobial peptides (AMPs) in the fat body (the equivalent of the mammalian liver) and the cellular response mediated by haemocytes (blood cells) engaged in phagocytosis represent two major reactions that counter pathogens. Although considerable analysis has permitted the elucidation of mechanisms pertaining to the two responses individually, the mechanism of their coordination has been unclear. To characterise the signals with which infection might be communicated between blood cells and fat body, we ablated circulating haemocytes and defined the parameters of AMP gene activation in larvae. We found that targeted ablation of blood cells influenced the levels of AMP gene expression in the fat body following both septic injury and oral infection. Expression of the AMP gene drosomycin (a Toll target) was blocked when expression of the Toll ligand Spätzle was knocked down in haemocytes. These results show that in larvae, integration of the two responses in a systemic reaction depend on the production of a cytokine (spz), a process that strongly parallels the mammalian immune response.},
keywords = {Animals, Enterococcus faecalis, Escherichia coli, Fat Body, Hemocytes, Larva, M3i, reichhart, Toll-Like Receptors},
pubstate = {published},
tppubtype = {article}
}
Cronin Shane J F, Nehme Nadine T, Limmer Stefanie, Liegeois Samuel, Pospisilik Andrew J, Schramek Daniel, Leibbrandt Andreas, de Simoes Ricardo Matos, Gruber Susanne, Puc Urszula, Ebersberger Ingo, Zoranovic Tamara, Neely Gregory G, von Haeseler Arndt, Ferrandon Dominique, Penninger Josef M
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection Article de journal
Dans: Science, vol. 325, no. 5938, p. 340–343, 2009, ISSN: 1095-9203.
Résumé | Liens | BibTeX | Étiquettes: *Genome, *RNA Interference, Animal, Animals, Cell Proliferation, Drosophila melanogaster/*genetics/immunology/*microbiology, Drosophila Proteins/genetics/metabolism, Epithelial Cells, Epithelial Cells/cytology/physiology, ferrandon, Genetically Modified, Genome, Hemocytes, Hemocytes/immunology/metabolism/microbiology, Homeostasis, Immunity, Innate, Innate/*genetics, Insect, Intestinal Mucosa, Intestinal Mucosa/cytology/immunology/metabolism/microbiology, Janus Kinases, Janus Kinases/genetics/metabolism, M3i, Models, RNA Interference, Serratia Infections, Serratia Infections/genetics/*immunology/microbiology, Serratia marcescens, Serratia marcescens/*immunology/physiology, Signal Transduction, STAT Transcription Factors, STAT Transcription Factors/genetics/metabolism, Stem Cells, Stem Cells/cytology/physiology
@article{cronin_genome-wide_2009b,
title = {Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection},
author = {Shane J F Cronin and Nadine T Nehme and Stefanie Limmer and Samuel Liegeois and Andrew J Pospisilik and Daniel Schramek and Andreas Leibbrandt and Ricardo Matos de Simoes and Susanne Gruber and Urszula Puc and Ingo Ebersberger and Tamara Zoranovic and Gregory G Neely and Arndt von Haeseler and Dominique Ferrandon and Josef M Penninger},
doi = {10.1126/science.1173164},
issn = {1095-9203},
year = {2009},
date = {2009-01-01},
journal = {Science},
volume = {325},
number = {5938},
pages = {340--343},
abstract = {Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.},
keywords = {*Genome, *RNA Interference, Animal, Animals, Cell Proliferation, Drosophila melanogaster/*genetics/immunology/*microbiology, Drosophila Proteins/genetics/metabolism, Epithelial Cells, Epithelial Cells/cytology/physiology, ferrandon, Genetically Modified, Genome, Hemocytes, Hemocytes/immunology/metabolism/microbiology, Homeostasis, Immunity, Innate, Innate/*genetics, Insect, Intestinal Mucosa, Intestinal Mucosa/cytology/immunology/metabolism/microbiology, Janus Kinases, Janus Kinases/genetics/metabolism, M3i, Models, RNA Interference, Serratia Infections, Serratia Infections/genetics/*immunology/microbiology, Serratia marcescens, Serratia marcescens/*immunology/physiology, Signal Transduction, STAT Transcription Factors, STAT Transcription Factors/genetics/metabolism, Stem Cells, Stem Cells/cytology/physiology},
pubstate = {published},
tppubtype = {article}
}
2005
Irving Phil, Ubeda Jean-Michel, Doucet Daniel, Troxler Laurent, Lagueux Marie, Zachary Daniel, Hoffmann Jules A, Hetru Charles, Meister Marie
New insights into Drosophila larval haemocyte functions through genome-wide analysis Article de journal
Dans: Cell. Microbiol., vol. 7, no. 3, p. 335–350, 2005, ISSN: 1462-5814.
Résumé | Liens | BibTeX | Étiquettes: Animals, bioinformatic, Catechol Oxidase, Cell Lineage, Enzyme Precursors, Escherichia coli, Fat Body, Gene Expression Profiling, Genome, Hemocytes, hoffmann, Integrin alpha Chains, Integrins, Larva, M3i, Micrococcus luteus
@article{irving_new_2005,
title = {New insights into Drosophila larval haemocyte functions through genome-wide analysis},
author = {Phil Irving and Jean-Michel Ubeda and Daniel Doucet and Laurent Troxler and Marie Lagueux and Daniel Zachary and Jules A Hoffmann and Charles Hetru and Marie Meister},
doi = {10.1111/j.1462-5822.2004.00462.x},
issn = {1462-5814},
year = {2005},
date = {2005-03-01},
journal = {Cell. Microbiol.},
volume = {7},
number = {3},
pages = {335--350},
abstract = {Drosophila blood cells or haemocytes comprise three cell lineages, plasmatocytes, crystal cells and lamellocytes, involved in immune functions such as phagocytosis, melanisation and encapsulation. Transcriptional profiling of activities of distinct haemocyte populations and from naive or infected larvae, was performed to find genes contributing to haemocyte functions. Of the 13 000 genes represented on the microarray, over 2500 exhibited significantly enriched transcription in haemocytes. Among these were genes encoding integrins, peptidoglycan recognition proteins (PGRPs), scavenger receptors, lectins, cell adhesion molecules and serine proteases. One relevant outcome of this analysis was the gain of new insights into the lamellocyte encapsulation process. We showed that lamellocytes require betaPS integrin for encapsulation and that they transcribe one prophenoloxidase gene enabling them to produce the enzyme necessary for melanisation of the capsule. A second compelling observation was that following infection, the gene encoding the cytokine Spatzle was uniquely upregulated in haemocytes and not the fat body. This shows that Drosophila haemocytes produce a signal molecule ready to be activated through cleavage after pathogen recognition, informing distant tissues of infection.},
keywords = {Animals, bioinformatic, Catechol Oxidase, Cell Lineage, Enzyme Precursors, Escherichia coli, Fat Body, Gene Expression Profiling, Genome, Hemocytes, hoffmann, Integrin alpha Chains, Integrins, Larva, M3i, Micrococcus luteus},
pubstate = {published},
tppubtype = {article}
}
2004
Blandin Stéphanie A, Levashina Elena A
Mosquito immune responses against malaria parasites Article de journal
Dans: Curr. Opin. Immunol., vol. 16, no. 1, p. 16–20, 2004, ISSN: 0952-7915.
Résumé | BibTeX | Étiquettes: Animals, Anopheles, blandin, Gene Library, Genes, Hemocytes, Host-Parasite Interactions, Immunity, Innate, Insect, Insect Vectors, M3i, Malaria, Plasmodium
@article{blandin_mosquito_2004,
title = {Mosquito immune responses against malaria parasites},
author = {Stéphanie A Blandin and Elena A Levashina},
issn = {0952-7915},
year = {2004},
date = {2004-01-01},
journal = {Curr. Opin. Immunol.},
volume = {16},
number = {1},
pages = {16--20},
abstract = {Anopheline mosquitoes are the major vectors of human malaria. Mosquito-parasite interactions are a critical aspect of disease transmission and a potential target for malaria control. Mosquitoes vary in their innate ability to support development of the malaria parasite, but the molecular mechanisms that determine vector competence are poorly understood. This area of research has been revolutionized by recent advances in the mosquito genome characterization and by the development of new tools for functional gene analysis.},
keywords = {Animals, Anopheles, blandin, Gene Library, Genes, Hemocytes, Host-Parasite Interactions, Immunity, Innate, Insect, Insect Vectors, M3i, Malaria, Plasmodium},
pubstate = {published},
tppubtype = {article}
}
2002
Munier Anne-Isabelle, Doucet Daniel, Perrodou Emmanuel, Zachary Daniel, Meister Marie, Hoffmann Jules A, Janeway Charles A, Lagueux Marie
PVF2, a PDGF/VEGF-like growth factor, induces hemocyte proliferation in Drosophila larvae Article de journal
Dans: EMBO Rep., vol. 3, no. 12, p. 1195–1200, 2002, ISSN: 1469-221X.
Résumé | Liens | BibTeX | Étiquettes: Animals, Antibodies, Blotting, Cell Differentiation, Hemocytes, hoffmann, Immunohistochemistry, Larva, ligands, M3i, Platelet-Derived Growth Factor, Receptors, Vascular Endothelial Growth Factor, Western
@article{munier_pvf2_2002,
title = {PVF2, a PDGF/VEGF-like growth factor, induces hemocyte proliferation in Drosophila larvae},
author = {Anne-Isabelle Munier and Daniel Doucet and Emmanuel Perrodou and Daniel Zachary and Marie Meister and Jules A Hoffmann and Charles A Janeway and Marie Lagueux},
doi = {10.1093/embo-reports/kvf242},
issn = {1469-221X},
year = {2002},
date = {2002-12-01},
journal = {EMBO Rep.},
volume = {3},
number = {12},
pages = {1195--1200},
abstract = {Blood cells play a crucial role in both morphogenetic and immunological processes in Drosophila, yet the factors regulating their proliferation remain largely unknown. In order to address this question, we raised antibodies against a tumorous blood cell line and identified an antigenic determinant that marks the surface of prohemocytes and also circulating plasmatocytes in larvae. This antigen was identified as a Drosophila homolog of the mammalian receptor for platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF). The Drosophila receptor controls cell proliferation in vitro. By overexpressing in vivo one of its putative ligands, PVF2, we induced a dramatic increase in circulating hemocytes. These results identify the PDGF/VEGF receptor homolog and one of its ligands as important players in Drosophila hematopoiesis.},
keywords = {Animals, Antibodies, Blotting, Cell Differentiation, Hemocytes, hoffmann, Immunohistochemistry, Larva, ligands, M3i, Platelet-Derived Growth Factor, Receptors, Vascular Endothelial Growth Factor, Western},
pubstate = {published},
tppubtype = {article}
}
2001
Boulanger Nathalie, Ehret-Sabatier Laurence, Brun R, Zachary Daniel, Bulet Philippe, Imler Jean-Luc
Immune response of Drosophila melanogaster to infection with the flagellate parasite Crithidia spp Article de journal
Dans: Insect Biochemistry and Molecular Biology, vol. 31, no. 2, p. 129–137, 2001, ISSN: 0965-1748.
Résumé | BibTeX | Étiquettes: Animals, Anti-Bacterial Agents, Antimicrobial Cationic Peptides, Crithidia, Defensins, Gene Expression, Glycopeptides, Hemocytes, imler, Insect Proteins, M3i, Phagocytosis
@article{boulanger_immune_2001,
title = {Immune response of Drosophila melanogaster to infection with the flagellate parasite Crithidia spp},
author = {Nathalie Boulanger and Laurence Ehret-Sabatier and R Brun and Daniel Zachary and Philippe Bulet and Jean-Luc Imler},
issn = {0965-1748},
year = {2001},
date = {2001-02-01},
journal = {Insect Biochemistry and Molecular Biology},
volume = {31},
number = {2},
pages = {129--137},
abstract = {Insects are able to recognize invading microorganisms and to mount an immune response to bacterial and fungal infections. Recently, the fruitfly Drosophila melanogaster has emerged as a promising invertebrate model to investigate innate immunity because of its well-characterized genetics. Insects are also vectors of numerous parasites which can trigger an immune response. We have investigated the interaction of Drosophila melanogaster with the flagellate protozoan Crithidia spp. We show that a per os parasitic infection triggers the synthesis of several antimicrobial peptides. By reverse phase HPLC and mass spectrometry, peptides were shown to be present in the hemolymph and not in the gut tissue, suggesting the presence of immune messengers between the site of the infection, namely the gut, and the fat body, the main site of synthesis for antimicrobial peptides. Interestingly, we have identified one molecule which is specifically induced in the hemolymph after infection with Crithidia, but not with bacteria, suggesting that Drosophila can discriminate between pathogens. When flagellates were injected into the hemolymph, a low synthesis of antimicrobial peptides was observed together with phagocytosis of parasites by circulating hemocytes. The data presented here suggest that Drosophila-Crithidia spp. represents an interesting model to study host defense against protozoan parasites.},
keywords = {Animals, Anti-Bacterial Agents, Antimicrobial Cationic Peptides, Crithidia, Defensins, Gene Expression, Glycopeptides, Hemocytes, imler, Insect Proteins, M3i, Phagocytosis},
pubstate = {published},
tppubtype = {article}
}
Levashina Elena A, Moita L F, Blandin Stéphanie A, Vriend G, Lagueux Marie, Kafatos F C
Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae Article de journal
Dans: Cell, vol. 104, no. 5, p. 709–718, 2001, ISSN: 0092-8674.
Résumé | BibTeX | Étiquettes: alpha-Macroglobulins, Animals, Anopheles, blandin, Cells, Cloning, Complement C3, Cultured, DNA Fragmentation, Double-Stranded, Female, Genetic, Gram-Negative Bacteria, Hemocytes, Insect Proteins, M3i, Molecular, Nucleic Acid Denaturation, Phagocytosis, Protein Structure, RNA, Tertiary, Transcription
@article{levashina_conserved_2001,
title = {Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae},
author = {Elena A Levashina and L F Moita and Stéphanie A Blandin and G Vriend and Marie Lagueux and F C Kafatos},
issn = {0092-8674},
year = {2001},
date = {2001-01-01},
journal = {Cell},
volume = {104},
number = {5},
pages = {709--718},
abstract = {We characterize a novel hemocyte-specific acute phase glycoprotein from the malaria vector, Anopheles gambiae. It shows substantial structural and functional similarities, including the highly conserved thioester motif, to both a central component of mammalian complement system, factor C3, and to a pan-protease inhibitor, alpha2-macroglobulin. Most importantly, this protein serves as a complement-like opsonin and promotes phagocytosis of some Gram-negative bacteria in a mosquito hemocyte-like cell line. Chemical inactivation by methylamine and depletion by double-stranded RNA knockout demonstrate that this function is dependent on the internal thioester bond. This evidence of a complement-like function in a protostome animal adds substantially to the accumulating evidence of a common ancestry of immune defenses in insects and vertebrates.},
keywords = {alpha-Macroglobulins, Animals, Anopheles, blandin, Cells, Cloning, Complement C3, Cultured, DNA Fragmentation, Double-Stranded, Female, Genetic, Gram-Negative Bacteria, Hemocytes, Insect Proteins, M3i, Molecular, Nucleic Acid Denaturation, Phagocytosis, Protein Structure, RNA, Tertiary, Transcription},
pubstate = {published},
tppubtype = {article}
}
1998
Braun A, Hoffmann Jules A, Meister Marie
Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes Article de journal
Dans: Proc. Natl. Acad. Sci. U.S.A., vol. 95, no. 24, p. 14337–14342, 1998, ISSN: 0027-8424.
Résumé | BibTeX | Étiquettes: Adipose Tissue, Animals, Candida, Escherichia coli, Fungal, Genotype, Hemocytes, hoffmann, Larva, M3i, Melanins, Micrococcus luteus, Spores
@article{braun_analysis_1998,
title = {Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes},
author = {A Braun and Jules A Hoffmann and Marie Meister},
issn = {0027-8424},
year = {1998},
date = {1998-11-01},
journal = {Proc. Natl. Acad. Sci. U.S.A.},
volume = {95},
number = {24},
pages = {14337--14342},
abstract = {We have analyzed the Drosophila immune response in domino mutant larvae, which are devoid of blood cells. The domino mutants have a good larval viability, but they die as prepupae. We show that, on immune challenge, induction of the genes encoding antimicrobial peptides in the fat body is not affected significantly in the mutant larvae, indicating that hemocytes are not essential in this process. The hemocoele of domino larvae contains numerous live microorganisms, the presence of which induces a weak antimicrobial response in the fat body. A full response is observed only after septic injury. We propose that the fat body cells are activated both by the presence of microorganisms and by injury and that injury potentiates the effect of microorganisms. Survival experiments after an immune challenge showed that domino mutants devoid of blood cells maintain a wild-type resistance to septic injury. This resistance was also observed in mutant larvae in which the synthesis of antibacterial peptides is impaired (immune deficiency larvae) and in mutants that are deficient for humoral melanization (Black cells larvae). However, if domino was combined with either the immune deficiency or the Black cell mutation, the resistance to septic injury was reduced severely. These results establish the relevance of the three immune reactions: phagocytosis, synthesis of antibacterial peptides, and melanization. By working in synergy, they provide Drosophila a highly effective defense against injury and/or infection.},
keywords = {Adipose Tissue, Animals, Candida, Escherichia coli, Fungal, Genotype, Hemocytes, hoffmann, Larva, M3i, Melanins, Micrococcus luteus, Spores},
pubstate = {published},
tppubtype = {article}
}
1997
Dimarcq Jean-Luc, Imler Jean-Luc, Lanot R, Ezekowitz Alan R B, Hoffmann Jules A, Janeway C A, Lagueux Marie
Treatment of l(2)mbn Drosophila tumorous blood cells with the steroid hormone ecdysone amplifies the inducibility of antimicrobial peptide gene expression Article de journal
Dans: Insect Biochemistry and Molecular Biology, vol. 27, no. 10, p. 877–886, 1997, ISSN: 0965-1748.
Résumé | BibTeX | Étiquettes: Animals, Bacterial Infections, Cellular, Ecdysone, Gene Expression, Genes, Hemocytes, Hemolymph, hoffmann, imler, Immunity, Insect, M3i, Macrophages, Peptide Biosynthesis, Phagocytosis
@article{dimarcq_treatment_1997,
title = {Treatment of l(2)mbn Drosophila tumorous blood cells with the steroid hormone ecdysone amplifies the inducibility of antimicrobial peptide gene expression},
author = {Jean-Luc Dimarcq and Jean-Luc Imler and R Lanot and Alan R B Ezekowitz and Jules A Hoffmann and C A Janeway and Marie Lagueux},
issn = {0965-1748},
year = {1997},
date = {1997-10-01},
journal = {Insect Biochemistry and Molecular Biology},
volume = {27},
number = {10},
pages = {877--886},
abstract = {Insects rely on both humoral and cellular mechanisms to defend themselves against microbial infections. The humoral response involves synthesis of a battery of potent antimicrobial peptides by the fat body and, to a lesser extent, by blood cells. The cellular response on the other hand consists of phagocytosis of small microorganisms and melanization and encapsulation of larger parasites. The l(2)mbn cell line, established from tumorous larval hemocytes, represents a system of choice to dissect the molecular events controlling cellular immunity. We report here that l(2)mbn cells can be efficiently induced to differentiate in adherent, macrophage-like cells by treatment with 20-hydroxyecdysone. Ecdysone treatment increases both the phagocytic capacity of l(2)mbn cells and their competence to express antimicrobial genes in response to immune challenge. We also report that expression of several regulatory molecules thought to be involved in the immune response is up-regulated by ecdysone in l(2)mbn cells.},
keywords = {Animals, Bacterial Infections, Cellular, Ecdysone, Gene Expression, Genes, Hemocytes, Hemolymph, hoffmann, imler, Immunity, Insect, M3i, Macrophages, Peptide Biosynthesis, Phagocytosis},
pubstate = {published},
tppubtype = {article}
}
1978
Brehélin M, Zachary Daniel, Hoffmann Jules A
A comparative ultrastructural study of blood cells from nine insect orders Article de journal
Dans: Cell Tissue Res., vol. 195, no. 1, p. 45–57, 1978, ISSN: 0302-766X.
Résumé | BibTeX | Étiquettes: Animals, Blood Cells, Electron, Granulocytes, Hemocytes, hoffmann, insects, M3i, Microscopy, Species Specificity
@article{brehelin_comparative_1978,
title = {A comparative ultrastructural study of blood cells from nine insect orders},
author = {M Brehélin and Daniel Zachary and Jules A Hoffmann},
issn = {0302-766X},
year = {1978},
date = {1978-01-01},
journal = {Cell Tissue Res.},
volume = {195},
number = {1},
pages = {45--57},
abstract = {An ultrastructural study of hemocytes from 9 different insect orders has led to the identification of 8 cell types: (1) Plasmatocytes, whose cytoplasm is filled with small dense lysosomes and large heterogeneous structures, are phagocytic cells. (2) Granulocytes, filled with uniformly electron dense granules, are involved in capsule formation. (3) Coagulocytes, which contain granules and structured globules and which possess a well developed RER, are involved in phagocytosis. (4) Spherule cells are filled with large spherical inclusions. (5) Oenocytoids are large cells with few cytoplasmic organelles. These 5 hemocyte types represent the majority of insect blood cells. (6) Prohemocytes, blastic cells which are one of the stem cells a hemocytes, are very few in number in each species investigated. (7) Thrombocytoids and (8) Prodocytes are restricted to a small number of insect species. The ultrastructural characteristics of these hemocyte types are discussed.},
keywords = {Animals, Blood Cells, Electron, Granulocytes, Hemocytes, hoffmann, insects, M3i, Microscopy, Species Specificity},
pubstate = {published},
tppubtype = {article}
}
1977
Brehelin M, Zachary Daniel, Hoffmann Jules A
Comparative study of hemocyte capsule formation in Locusta migratoria, Melolontha melolontha and Calliphora erythrocephala Article de journal
Dans: Ann Parasitol Hum Comp, vol. 52, no. 1, p. 66–67, 1977, ISSN: 0003-4150.
BibTeX | Étiquettes: Animals, Beetles, Blood Cells, Cellular, Diptera, Grasshoppers, Hemocytes, hoffmann, Immunity, M3i
@article{brehelin_comparative_1977,
title = {Comparative study of hemocyte capsule formation in Locusta migratoria, Melolontha melolontha and Calliphora erythrocephala},
author = {M Brehelin and Daniel Zachary and Jules A Hoffmann},
issn = {0003-4150},
year = {1977},
date = {1977-02-01},
journal = {Ann Parasitol Hum Comp},
volume = {52},
number = {1},
pages = {66--67},
keywords = {Animals, Beetles, Blood Cells, Cellular, Diptera, Grasshoppers, Hemocytes, hoffmann, Immunity, M3i},
pubstate = {published},
tppubtype = {article}
}
Hoffmann Danièle, Brehelin M, Hoffmann Jules A
First results of the antibacterial defense reactions of Locusta migratoria larva and imago Article de journal
Dans: Ann Parasitol Hum Comp, vol. 52, no. 1, p. 87–88, 1977, ISSN: 0003-4150.
BibTeX | Étiquettes: Animals, Bacillus thuringiensis, bacteria, Cellular, Grasshoppers, Hematopoietic System, Hemocytes, Hemolymph, hoffmann, Immunity, Larva, M3i, Muramidase
@article{hoffmann_first_1977,
title = {First results of the antibacterial defense reactions of Locusta migratoria larva and imago},
author = {Danièle Hoffmann and M Brehelin and Jules A Hoffmann},
issn = {0003-4150},
year = {1977},
date = {1977-02-01},
journal = {Ann Parasitol Hum Comp},
volume = {52},
number = {1},
pages = {87--88},
keywords = {Animals, Bacillus thuringiensis, bacteria, Cellular, Grasshoppers, Hematopoietic System, Hemocytes, Hemolymph, hoffmann, Immunity, Larva, M3i, Muramidase},
pubstate = {published},
tppubtype = {article}
}