Publications
2012
Peltier Claire, Klein Elodie, Hleibieh Kamal, D'Alonzo Massimiliano, Hammann Philippe, Bouzoubaa Salah, Ratti Claudio, Gilmer David
Beet necrotic yellow vein virus subgenomic RNA3 is a cleavage product leading to stable non-coding RNA required for long-distance movement. Article de journal
Dans: The Journal of general virology, vol. 93, no. Pt 5, p. 1093–1102, 2012, ISSN: 1465-2099 0022-1317, (Place: England).
Résumé | Liens | BibTeX | Étiquettes: *Genome, Arabidopsis/virology, Beta vulgaris/virology, Plant Viruses/genetics/*pathogenicity, PPSE, RNA, RNA Stability, Tobacco/virology, Untranslated/*metabolism, Viral, Viral/genetics/*metabolism
@article{peltier_beet_2012,
title = {Beet necrotic yellow vein virus subgenomic RNA3 is a cleavage product leading to stable non-coding RNA required for long-distance movement.},
author = {Claire Peltier and Elodie Klein and Kamal Hleibieh and Massimiliano D'Alonzo and Philippe Hammann and Salah Bouzoubaa and Claudio Ratti and David Gilmer},
doi = {10.1099/vir.0.039685-0},
issn = {1465-2099 0022-1317},
year = {2012},
date = {2012-01-01},
journal = {The Journal of general virology},
volume = {93},
number = {Pt 5},
pages = {1093--1102},
abstract = {Beet necrotic yellow vein virus (BNYVV) is a multipartite RNA virus. BNYVV RNA3 does not accumulate in non-host transgenic Arabidopsis thaliana plants when expressed using a 35S promoter. However, a 3'-derivative species has been detected in transgenic plants and in transient expression assays conducted in Nicotiana benthamiana and Beta macrocarpa. The 3'-derivative species is similar to the previously reported subgenomic RNA3 produced during virus infection. 5' RACE revealed that the truncated forms had identical 5' ends. The 5' termini carried the coremin motif also present on BNYVV RNA5, beet soil-borne mosaic virus RNA3 and 4, and cucumber mosaic virus group 2 RNAs. This RNA3 species lacks a m(7)Gppp at the 5' end of the cleavage products, whether expressed transiently or virally. Mutagenesis revealed the importance of the coremin sequence for both long-distance movement and stabilization of the cleavage product in vivo and in vitro. The isolation of various RNA3 5'-end products suggests the existence of a cleavage between nt 212 and 1234 and subsequent exonucleolytic degradation, leading to the accumulation of a non-coding RNA. When RNA3 was incubated in wheatgerm extracts, truncated forms appeared rapidly and their appearance was protein- and divalent ion-dependent.},
note = {Place: England},
keywords = {*Genome, Arabidopsis/virology, Beta vulgaris/virology, Plant Viruses/genetics/*pathogenicity, PPSE, RNA, RNA Stability, Tobacco/virology, Untranslated/*metabolism, Viral, Viral/genetics/*metabolism},
pubstate = {published},
tppubtype = {article}
}
2009
Cronin Shane J F, Nehme Nadine T, Limmer Stefanie, Liegeois Samuel, Pospisilik Andrew J, Schramek Daniel, Leibbrandt Andreas, de Simoes Ricardo Matos, Gruber Susanne, Puc Urszula, Ebersberger Ingo, Zoranovic Tamara, Neely Gregory G, von Haeseler Arndt, Ferrandon Dominique, Penninger Josef M
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection Article de journal
Dans: Science, vol. 325, no. 5938, p. 340–343, 2009, ISSN: 1095-9203.
Résumé | Liens | BibTeX | Étiquettes: *Genome, *RNA Interference, Animal, Animals, Cell Proliferation, Drosophila melanogaster/*genetics/immunology/*microbiology, Drosophila Proteins/genetics/metabolism, Epithelial Cells, Epithelial Cells/cytology/physiology, ferrandon, Genetically Modified, Genome, Hemocytes, Hemocytes/immunology/metabolism/microbiology, Homeostasis, Immunity, Innate, Innate/*genetics, Insect, Intestinal Mucosa, Intestinal Mucosa/cytology/immunology/metabolism/microbiology, Janus Kinases, Janus Kinases/genetics/metabolism, M3i, Models, RNA Interference, Serratia Infections, Serratia Infections/genetics/*immunology/microbiology, Serratia marcescens, Serratia marcescens/*immunology/physiology, Signal Transduction, STAT Transcription Factors, STAT Transcription Factors/genetics/metabolism, Stem Cells, Stem Cells/cytology/physiology
@article{cronin_genome-wide_2009b,
title = {Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection},
author = {Shane J F Cronin and Nadine T Nehme and Stefanie Limmer and Samuel Liegeois and Andrew J Pospisilik and Daniel Schramek and Andreas Leibbrandt and Ricardo Matos de Simoes and Susanne Gruber and Urszula Puc and Ingo Ebersberger and Tamara Zoranovic and Gregory G Neely and Arndt von Haeseler and Dominique Ferrandon and Josef M Penninger},
doi = {10.1126/science.1173164},
issn = {1095-9203},
year = {2009},
date = {2009-01-01},
journal = {Science},
volume = {325},
number = {5938},
pages = {340--343},
abstract = {Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.},
keywords = {*Genome, *RNA Interference, Animal, Animals, Cell Proliferation, Drosophila melanogaster/*genetics/immunology/*microbiology, Drosophila Proteins/genetics/metabolism, Epithelial Cells, Epithelial Cells/cytology/physiology, ferrandon, Genetically Modified, Genome, Hemocytes, Hemocytes/immunology/metabolism/microbiology, Homeostasis, Immunity, Innate, Innate/*genetics, Insect, Intestinal Mucosa, Intestinal Mucosa/cytology/immunology/metabolism/microbiology, Janus Kinases, Janus Kinases/genetics/metabolism, M3i, Models, RNA Interference, Serratia Infections, Serratia Infections/genetics/*immunology/microbiology, Serratia marcescens, Serratia marcescens/*immunology/physiology, Signal Transduction, STAT Transcription Factors, STAT Transcription Factors/genetics/metabolism, Stem Cells, Stem Cells/cytology/physiology},
pubstate = {published},
tppubtype = {article}
}
1995
Keith G., Dirheimer G.
Postlabeling: a sensitive method for studying DNA adducts and their role in carcinogenesis Article de journal
Dans: Curr Opin Biotechnol, vol. 6, no. 1, p. 3-11, 1995, (0958-1669 Journal Article Review Review, Academic).
Résumé | BibTeX | Étiquettes: *Cell, *Genome, Adducts/*analysis, and, Animals, Base, Cell, Conditions/genetics/pathology, Data, Dilution, Division, DNA, Genetic, Gov't, Human, Models, Molecular, Mutagenesis, Neoplasms/*genetics/pathology, Neoplastic, Non-U.S., Phosphorus, Precancerous, Radioisotope, Radioisotopes, Sensitivity, Sequence, Specificity, Support, Technique, Transformation, Xenobiotics
@article{,
title = {Postlabeling: a sensitive method for studying DNA adducts and their role in carcinogenesis},
author = { G. Keith and G. Dirheimer},
year = {1995},
date = {1995-01-01},
journal = {Curr Opin Biotechnol},
volume = {6},
number = {1},
pages = {3-11},
abstract = {The covalent binding of xenobiotics to DNA is an important trigger of the multistage process that leads to carcinogenesis. 32P-postlabeling represents a highly sensitive method for biomonitoring exposure to genotoxic agents and for cancer risk assessment; it is capable of detecting less than one DNA adduct per human genome. Recent improvements to the technique have shown that the resistance of adducted DNA to enzyme digestion may lead to an overestimation of the number of different adducts present in a sample.},
note = {0958-1669
Journal Article
Review
Review, Academic},
keywords = {*Cell, *Genome, Adducts/*analysis, and, Animals, Base, Cell, Conditions/genetics/pathology, Data, Dilution, Division, DNA, Genetic, Gov't, Human, Models, Molecular, Mutagenesis, Neoplasms/*genetics/pathology, Neoplastic, Non-U.S., Phosphorus, Precancerous, Radioisotope, Radioisotopes, Sensitivity, Sequence, Specificity, Support, Technique, Transformation, Xenobiotics},
pubstate = {published},
tppubtype = {article}
}