Biologie Digitale de l’ARN

@article{,

title = {Transfer RNA binding protein in the nucleus of Saccharomyces cerevisiae},

author = { M. L. Wilhelm and J. Reinbolt and J. Gangloff and G. Dirheimer and F. X. Wilhelm},

year  = {1994},

date = {1994-01-01},

journal = {FEBS Lett},

volume = {349},

number = {2},

pages = {260-4},

abstract = {A yeast nuclear protein that binds to tRNA was identified using a RNA mobility shift assay. Northwestern blotting and N-terminal sequencing experiments indicate that this tRNA-binding protein is identical to zuotin which has previously been shown to bind to Z-DNA [(1992) EMBO J. 11, 3787-3796]. Labeled tRNA and poly(dG-m5dC) stabilized in the Z-DNA form identify the same protein on a Northwestern blot. In a gel retardation assay poly(dG-m5dC) in the Z-form strongly diminishes the binding of tRNA to zuotin. These studies establish that zuotin is able to bind to both tRNA and Z-DNA. Zuotin may be transiently associated with tRNA in the nucleus of yeast cells and play a role in its processing or transport to the cytoplasm.},

note = {0014-5793 

Journal Article},

keywords = {*Saccharomyces, &, Acid, Amino, Cell, cerevisiae, cerevisiae/*metabolism, Chromatography, Data, DNA-Binding, DNA/metabolism, Fungal, Fungal/*isolation, high, liquid, Molecular, Nucleus/*metabolism, Pressure, Proteins, Proteins/genetics/*metabolism, purification, RNA, Saccharomyces, Sequence, Transfer/*isolation},

pubstate = {published},

tppubtype = {article}

}