2-year post-doctoral fellowship
September 1, 2020
December 1, 2020
September 1, 2020
Role of Shigellaand Salmonellavirulence factors in neutrophil activation and cell-death induction.
Upon Shigellaor Salmonellainfections, neutrophilsare the most abundant immune cells recruited to foci of infections. Our team has previously demonstrated that these interactions occur mainly in low-oxygen conditions, induced by pathogenaerobic respiration. The expressionand activity of Shigellaand Salmonellasecretion systems(T3SS, T5SS)are tighly regulated by oxygen(Marteyn, 2010) (Tinevez, 2019). Neutrophilviability is maintained under low-oxygen conditions (Monceaux, 2016).In this project, we aim at deciphering how oxygen modulatesinteractions betweenpathogenic bacteria and neutrophils. In more details, we will investigate howShigellaand SalmonellaT3SS and T5SSinduce neutrophil activation (inflammasomeactivation, phagocytosis, degranulation, NET formation)or cell-death(apoptosis, pyroptosis).Experiments will be conducted under controlledoxygen levels (0, 1, 4% O2) to reflect various conditions potentially encountered in vivo; particularlyin mice and guinea pigs, which are the animal models of salmonellosis and shigellosis used in routine in our laboratory.
Tinevez JY, Arena ET, Anderson M, Nigro G, Injarabian L, Andre AC, Ferrari M, Campbell-Valois FX, Devin A, Shorte SL, Sansonetti PJ, Marteyn BS. Enteropathogen mediated oxygen depletion is essential for intestinal mucosacolonization. Nat. Micro.2019 nov 4 (11), 2001-2009.
Monceaux V, Chiche-Lapierre C, Chaput C, Witko-Sarsat V, Prevost MC, Taylor CT, Ungeheuer MN, Sansonetti PJ, Marteyn BS, Anoxia and glucose supplementation maintain neutrophil viability and increase transfusion and transfection efficiency, Blood, 2016 Aug 18;128(7):993-1002
Marteyn B, West N, Browning D, Cole J, Shaw J, Palm F, Mounier J, Prevost MC, Sansonetti PJ and Tang CM, Modulation of Shigella virulence in response to available oxygen in vivo. Nature, 2010 May 20;465(7296):355-8
The “Pathogenesis of bacterial infections and immunity” team is housed at the Institute of Molecular and Cellular Biology (University of Strasbourg). The team is currently composed of three PhD students, one master student and one technician.
Shigella, Salmonella, neutrophil, oxygen
We are searching for a highly collaborative and inquisitive scientist to join our team. This position is a full-time, 2-year post-doctoral fellowship that requires previous experiencein host-pathogen interactionusing interdisciplinary techniques and knowledge in cellular and molecular biology.
This project will be conducted in collaboration with Pr. JonathanJantsch (University of Regensburg, Germany).
Documents to accompany your application
Contact informationDr. Benoit Marteyn, chef de l'équipe PII.
IBMC - UPR9002
CNRS / University of Strasbourg,
Team "Pathogenesis of bacterial infections and immunity" led by Dr. Benoit Marteyn