Imaging

@article{,

title = {Influence of C-5 halogenation of uridines on hairpin versus duplex RNA folding.},

author = {E Ennifar and S Bernacchi and P Wolff and P Dumas},

url = {http://www.ncbi.nlm.nih.gov/pubmed/17630326},

isbn = {17630326},

year  = {2007},

date = {2007-01-01},

journal = {RNA},

volume = {13},

number = {9},

pages = {1445-52},

abstract = {Halogenation of bases is a widespread method used for solving crystal structures of nucleic acids. However, this modification may have important consequences on RNA folding and thus on the success of crystallization. We have used a combination of UV thermal melting, steady-state fluorescence, X-ray crystallography, and gel electrophoresis techniques to study the influence of uridine halogenation (bromination or iodination) on the RNA folding. The HIV-1 Dimerization Initiation Site is an RNA hairpin that can adopt an alternative duplex conformation and was used as a model. We have shown that, unexpectedly, the RNA hairpin/duplex ratio is strongly dependent not only on the presence but also on the position of halogenation.},

keywords = {ENNIFAR, MARQUET, RNA  HIV  fluorescence  halogen  crystal, Unité ARN},

pubstate = {published},

tppubtype = {article}

}