Imaging

@inbook{,

title = {Modification of Selenoprotein mRNAs by Cap Tri-methylation},

author = {A S Gribling-Burrer and G Eriani and C Allmang},

editor = {L Chavatte},

url = {https://www.ncbi.nlm.nih.gov/pubmed/28917041?dopt=Abstract},

doi = {1007/978-1-4939-7258-6_9},

isbn = {28917041},

year  = {2018},

date = {2018-01-01},

booktitle = {Selenoproteins: Methods and Protocols},

volume = {1661},

pages = {125-141},

publisher = {Springer Protocols, Humana Press},

series = {Methods in Molecular Biology},

abstract = {Several selenoprotein mRNAs undergo 5' cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m32,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunoprecipitation methods for the detection of endogenous m32,2,7G-capped selenoprotein mRNAs from total cell extracts or after polysome fractionation of cytoplasmic extracts. We have also developed a method for the in vitro cap hypermethylation of selenoprotein mRNA transcripts using purified Tgs1 enzyme.},

keywords = {2, 7G cap, Cap hypermethylation Cap immunoprecipitation Selenoprotein mRNAs TMG cap Tgs1 m3 2, ERIANI, Unité ARN},

pubstate = {published},

tppubtype = {inbook}

}