Publications
2019
Meyer B, da Veiga C, Dumas P, Ennifar E
Thermodynamics of Molecular Machines Using Incremental ITC Book Chapter
In: Ennifar, E (Ed.): Microcalorimetry of Biological Molecules: Methods and Protocols, vol. 1964, pp. 129-140, Springer Protocols, Humana Press, New York, NY, 2019, ISBN: 30929240.
Abstract | Links | BibTeX | Tags: ENNIFAR, HIV reverse transcriptase Incremental ITC Mechanism of action Molecular biomachines Thermodynamics, Unité ARN
@inbook{,
title = {Thermodynamics of Molecular Machines Using Incremental ITC},
author = {B Meyer and C da Veiga and P Dumas and E Ennifar},
editor = {E Ennifar},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30929240},
doi = {10.1007/978-1-4939-9179-2_10},
isbn = {30929240},
year = {2019},
date = {2019-01-01},
booktitle = {Microcalorimetry of Biological Molecules: Methods and Protocols},
volume = {1964},
pages = {129-140},
publisher = {Springer Protocols, Humana Press},
address = {New York, NY},
series = {Methods in Molecular Biology},
abstract = {Molecular biomachines, such as DNA and RNA polymerases or the ribosome, are fascinating biological assemblies able to swiftly perform repeated and highly regulated tasks, with a remarkable accuracy. Significant advances in structural studies during the past 20 years provided a wealth of information regarding their architecture and considerably contributed to a better understanding of their mechanism of action. However, the three-dimensional structure of a biological nanomachine alone does not provide access to its detailed mechanism of action, even when obtained at atomic resolution. When combined with other biophysical approaches, thermodynamic data, together with kinetic data, are essential for a complete description of any binding interaction, revealing forces driving complex formation and providing insights into mechanisms of action. We have developed an incremental ITC approach that is well-suitable for analysis of biomolecular machines. This strategy allows a dissection of molecular biomachine reactions through successive additions in the ITC cell of consecutive substrates.},
keywords = {ENNIFAR, HIV reverse transcriptase Incremental ITC Mechanism of action Molecular biomachines Thermodynamics, Unité ARN},
pubstate = {published},
tppubtype = {inbook}
}
Molecular biomachines, such as DNA and RNA polymerases or the ribosome, are fascinating biological assemblies able to swiftly perform repeated and highly regulated tasks, with a remarkable accuracy. Significant advances in structural studies during the past 20 years provided a wealth of information regarding their architecture and considerably contributed to a better understanding of their mechanism of action. However, the three-dimensional structure of a biological nanomachine alone does not provide access to its detailed mechanism of action, even when obtained at atomic resolution. When combined with other biophysical approaches, thermodynamic data, together with kinetic data, are essential for a complete description of any binding interaction, revealing forces driving complex formation and providing insights into mechanisms of action. We have developed an incremental ITC approach that is well-suitable for analysis of biomolecular machines. This strategy allows a dissection of molecular biomachine reactions through successive additions in the ITC cell of consecutive substrates.