Imaging

@article{,

title = {Purification and Functional Characterization of a Biologically Active Full-Length Feline Immunodeficiency Virus (FIV) Pr50^{Gag}.},

author = {A Krishnan and V Pillai and A Chameettachal and L M Ali and F Nuzra Nagoor Pitchai and S Tariq and F Mustafa and R Marquet and T A Rizvi},

url = {https://www.ncbi.nlm.nih.gov/pubmed/31357656?report=&dispmax=200&tool=PubCrawler_2.23},

doi = {10.3390/v11080689},

isbn = {31357656},

year  = {2019},

date = {2019-01-01},

journal = {Viruses},

volume = {11},

number = {8},

pages = {689},

abstract = {The feline immunodeficiency virus (FIV) full-length Pr50Gag precursor is a key player in the assembly of new viral particles. It is also a critical component of the efficient selection and packaging of two copies of genomic RNA (gRNA) into the newly formed virus particles from a wide pool of cellular and spliced viral RNA. To understand the molecular mechanisms involved during FIV gRNA packaging, we expressed the His6-tagged and untagged recombinant FIV Pr50Gag protein both in eukaryotic and prokaryotic cells. The recombinant Pr50Gag-His6-tag fusion protein was purified from soluble fractions of prokaryotic cultures using immobilized metal affinity chromatography (IMAC). This purified protein was able to assemble in vitro into virus-like particles (VLPs), indicating that it preserved its ability to oligomerize/multimerize. Furthermore, VLPs formed in eukaryotic cells by the FIV full-length Pr50Gag both in the presence and absence of His6-tag could package FIV sub-genomic RNA to similar levels, suggesting that the biological activity of the recombinant full-length Pr50Gag fusion protein was retained in the presence of His6-tag at the carboxy terminus. Successful expression and purification of a biologically active, recombinant full-length Pr50Gag-His6-tag fusion protein will allow study of the intricate RNA-protein interactions involved during FIV gRNA encapsidation.},

keywords = {Gag protein purification His-tag fusion protein Pr50Gag protein expression feline immunodeficiency virus (FIV) retroviral RNA packaging viral assembly, MARQUET, PAILLART, Unité ARN},

pubstate = {published},

tppubtype = {article}

}