Imaging

@article{serag_functional_2011,

title = {Functional platform for controlled subcellular distribution of carbon nanotubes},

author = {Maged F Serag and Noritada Kaji and Enrica Venturelli and Yukihiro Okamoto and Kazuyoshi Terasaka and Manabu Tokeshi and Hajime Mizukami and Kevin Braeckmans and Alberto Bianco and Yoshinobu Baba},

doi = {10.1021/nn2035654},

issn = {1936-086X},

year  = {2011},

date = {2011-11-01},

journal = {ACS nano},

volume = {5},

number = {11},

pages = {9264--9270},

abstract = {As nanoparticles can cross different cellular barriers and access different tissues, control of their uptake and cellular fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show that the trafficking of single-walled carbon nanotubes (SWCNTs) through various subcellular membranes of the plant cell is facilitated or inhibited by attaching a suitable functional tag and controlling medium components. This enables a unique control over the uptake and the subcellular distribution of SWCNTs and provides a key strategy to promote their cellular elimination to minimize toxicity. Our results also demonstrate that SWCNTs are involved in a carrier-mediated transport (CMT) inside cells; this is a phenomenon that scientists could use to obtain novel molecular insights into CMT, with the potential translation to advances in subcellular nanobiology.},

keywords = {Biological Transport, carbon, Catharanthus, Exocytosis, Fluorescence Recovery After Photobleaching, Fluorescent Dyes, I2CT, Intracellular Space, Nanotubes, Surface Properties, Team-Bianco, Vacuoles},

pubstate = {published},

tppubtype = {article}

}

@article{serag_trafficking_2011,

title = {Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells},

author = {Maged F Serag and Noritada Kaji and Claire Gaillard and Yukihiro Okamoto and Kazuyoshi Terasaka and Mohammad Jabasini and Manabu Tokeshi and Hajime Mizukami and Alberto Bianco and Yoshinobu Baba},

doi = {10.1021/nn102344t},

issn = {1936-086X},

year  = {2011},

date = {2011-01-01},

journal = {ACS nano},

volume = {5},

number = {1},

pages = {493--499},

abstract = {Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (textbackslashtextless100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.},

keywords = {Biological Transport, carbon, Catharanthus, Cell Membrane, Endosomes, I2CT, Intracellular Space, Nanotubes, Protoplasts, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}