Imaging

@article{,

title = {An additional long-range interaction in human U1 snRNA},

author = {C Sturchler and P Carbon and A Krol},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1532853},

isbn = {1532853},

year  = {1992},

date = {1992-01-01},

journal = {Nucleic Acids Res},

volume = {20},

number = {6},

pages = {1215-1221},

abstract = {We present evidence for the existence of an additional long-range interaction in vertebrate U1 snRNAs. By submitting human U1 snRNP, HeLa nuclear extracts, authentic human or X. laevis in vitro transcribed U1 snRNAs to RNase V1, a nuclease specific for double-stranded regions, cleavages occurred in the sequence psi psi ACC (positions 5-9) residing in the 5' terminal region of the RNA. The RNase V1 sensitive region is insensitive to single-stranded probes, something unexpected knowing that it was considered single-stranded in order to base-pair to pre-mRNA 5' splice site. We have identified the sequence GGUAG (positions 132-136) as the only possible 3' partner. Mutants, either abolishing or restoring the interaction between the partners, coupled to an RNase V1 assay, served to substantiate this base-pairing model. The presence of this additional helix, even detected in nuclear extracts under in vitro splicing conditions, implies that a conformational change must occur to release a free U1 snRNA 5' end.},

note = {0305-1048 

Journal Article},

keywords = {Base Sequence  Binding Sites  Hela Cells  Human  Molecular Sequence Data  Mutagenesis, Site-Directed  Nucleic Acid Conformation  RNA, Small Nuclear, Small Nuclear/*chemistry/metabolism  Ribonucleases/metabolism  Ribonucleoproteins/metabolism  Ribonucleoproteins, Unité ARN},

pubstate = {published},

tppubtype = {article}

}