Publications
1997
Vlassov V, Abramova T, Godovikova T, Giege R, Silnikov V
Sequence-specific cleavage of yeast tRNA(Phe) with oligonucleotides conjugated to a diimidazole construct Journal Article
In: Antisense Nucleic Acid Drug Dev, vol. 7, no. 1, pp. 39-42, 1997, ISBN: 9055037, (1087-2906 Journal Article).
Abstract | Links | BibTeX | Tags: Antisense/chemical synthesis/metabolism/*pharmacology RNA, Base Sequence Imidazoles/*chemistry Molecular Sequence Data Oligonucleotides, Fungal/*drug effects RNA, Non-U.S. Gov't Yeasts/genetics, Pancreatic/pharmacology Support, Phe/chemistry/*drug effects/*metabolism Ribonuclease, Transfer, Unité ARN
@article{,
title = {Sequence-specific cleavage of yeast tRNA(Phe) with oligonucleotides conjugated to a diimidazole construct},
author = {V Vlassov and T Abramova and T Godovikova and R Giege and V Silnikov},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9055037},
isbn = {9055037},
year = {1997},
date = {1997-01-01},
journal = {Antisense Nucleic Acid Drug Dev},
volume = {7},
number = {1},
pages = {39-42},
abstract = {Oligonucleotide derivatives conjugated to a chemical construction with two histamine residues imitating the catalytic center of ribonuclease A have been synthesized. In experiments with the conjugates complementary to the 3'-end and to the variable loop and the T loop of yeast tRNA(Phe), it was shown that the compounds can accomplish sequence-specific cleavage of the target RNA in physiologic conditions.},
note = {1087-2906
Journal Article},
keywords = {Antisense/chemical synthesis/metabolism/*pharmacology RNA, Base Sequence Imidazoles/*chemistry Molecular Sequence Data Oligonucleotides, Fungal/*drug effects RNA, Non-U.S. Gov't Yeasts/genetics, Pancreatic/pharmacology Support, Phe/chemistry/*drug effects/*metabolism Ribonuclease, Transfer, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
1992
Przykorska A, el Adlouni C, Keith G, Szarkowski J W, Dirheimer G
Structural specificity of Rn nuclease I as probed on yeast tRNA(Phe) and tRNA(Asp) Journal Article
In: Nucleic Acids Res, vol. 20, no. 4, pp. 659-663, 1992, ISBN: 1542562, (0305-1048 Journal Article).
Abstract | Links | BibTeX | Tags: Asp/chemistry/genetics/*metabolism RNA, Base Composition Base Sequence Molecular Sequence Data Nucleic Acid Conformation RNA, Non-U.S. Gov't Yeasts/genetics, Pancreatic/*metabolism Secale cereale Substrate Specificity Support, Phe/chemistry/genetics/*metabolism Ribonuclease, Transfer, Unité ARN
@article{,
title = {Structural specificity of Rn nuclease I as probed on yeast tRNA(Phe) and tRNA(Asp)},
author = {A Przykorska and C el Adlouni and G Keith and J W Szarkowski and G Dirheimer},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1542562},
isbn = {1542562},
year = {1992},
date = {1992-01-01},
journal = {Nucleic Acids Res},
volume = {20},
number = {4},
pages = {659-663},
abstract = {A single-strand-specific nuclease from rye germ (Rn nuclease I) was characterized as a tool for secondary and tertiary structure investigation of RNAs. To test the procedure, yeast tRNA(Phe) and tRNA(Asp) for which the tertiary structures are known, as well as the 3'-half of tRNA(Asp) were used as substrates. In tRNA(Phe) the nuclease introduced main primary cuts at positions U33 and A35 of the anticodon loop and G18 and G19 of the D loop. No primary cuts were observed within the double stranded stems. In tRNA(Asp) the main cuts occurred at positions U33, G34, U35, C36 of the anticodon loop and G18 and C20:1 positions in the D loop. No cuts were observed in the T loop in intact tRNA(Asp) but strong primary cleavages occurred at positions psi 55, C56, A57 within that loop in the absence of the tertiary interactions between T and D loops (use of 3'-half tRNA(Asp)). These results show that Rn nuclease I is specific for exposed single-stranded regions.},
note = {0305-1048
Journal Article},
keywords = {Asp/chemistry/genetics/*metabolism RNA, Base Composition Base Sequence Molecular Sequence Data Nucleic Acid Conformation RNA, Non-U.S. Gov't Yeasts/genetics, Pancreatic/*metabolism Secale cereale Substrate Specificity Support, Phe/chemistry/genetics/*metabolism Ribonuclease, Transfer, Unité ARN},
pubstate = {published},
tppubtype = {article}
}