Imaging

@article{,

title = {Optimal tRNA((Ser)Sec) gene activity requires an upstream SPH motif},

author = {E Myslinski and A Krol and P Carbon},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1311068},

isbn = {1311068},

year  = {1992},

date = {1992-01-01},

journal = {Nucleic Acids Res},

volume = {20},

number = {2},

pages = {203-209},

abstract = {The X. laevis tRNA((Ser)Sec) gene is different from the other tRNA genes in that its promoter contains two external elements, a PSE and a TATA box functionally equivalent to those of the U6 snRNA gene. Of the two internal promoters governing classical tRNA gene transcription, only subsists the internal B box. In this report, we show that the tRNA((Ser)Sec) contains in addition an activator element (AE) which we have mapped by extensive mutagenesis. Activation is only dependent on a 15 bp fragment residing between -209 and -195 and containing an SPH motif. In vitro, this element forms a complex with a nuclear protein which is different from the TEF-1 transcriptional activator that binds the SV40 Sph motifs. This AE is versatile since it shows capacity of activating a variety of genes in vivo, including U1 and U6 snRNAs and HSV thymidine kinase. Unexpectedly for an snRNA-related gene, the tRNA((Ser)Sec) is deprived of octamer or octamer-like motifs. The X.laevis tRNA((Ser)Sec) gene represents the first example of a Pol III snRNA-type gene whose activation of transcription is completely octamer-independent.},

note = {0305-1048 

Journal Article},

keywords = {Amino Acid-Specific/*genetics  Recombinant Fusion Proteins/genetics/metabolism  Simian virus 40/*genetics  Support, Animals  Base Sequence  DNA Mutational Analysis  DNA-Binding Proteins/genetics  Enhancer Elements (Genetics)/*genetics/physiology  Gene Expression Regulation/*genetics  Molecular Sequence Data  Promoter Regions (Genetics)/genetics  RNA, Non-U.S. Gov't  TATA Box/genetics  Xenopus laevis/*genetics, Small Nuclear/genetics  RNA, Transfer, Unité ARN},

pubstate = {published},

tppubtype = {article}

}