Imaging

@article{,

title = {The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates},

author = {J Kondo and E Westhof},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18346970},

isbn = {18346970},

year  = {2008},

date = {2008-01-01},

journal = {Nucleic Acids Res},

volume = {36},

number = {8},

pages = {2654-2666},

abstract = {The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or bacteria have been solved. The resting 'off' state of the mitochondrial wild-type A site is surprisingly different from that of the bacterial A site. The mitochondrial A1555G mutant has two types of the 'off' states; one is similar to the mitochondrial wild-type 'off' state and the other is similar to the bacterial 'off' state. Our present results indicate that the dynamics of the A site in bacteria and mitochondria are different, a property probably related to the small number of tRNAs used for decoding in mitochondria. Based on these structures, we propose a hypothesis for the molecular mechanism of non-syndromic hearing loss due to the mitochondrial A1555G mutation.},

note = {1362-4962 (Electronic) 

Journal Article},

keywords = {Bacterial/*chemistry  RNA, Crystallography, Messenger/chemistry  RNA, Molecular  Nucleic Acid Conformation  Point Mutation  RNA/*chemistry/genetics  RNA, Ribosomal/*chemistry  RNA, Transfer/chemistry, Unité ARN, WESTHOF, WESTHOF  Crystallography, X-Ray  Hearing Loss/genetics  Humans  Models},

pubstate = {published},

tppubtype = {article}

}