Imaging

@article{dimarcq_treatment_1997,

title = {Treatment of l(2)mbn Drosophila tumorous blood cells with the steroid hormone ecdysone amplifies the inducibility of antimicrobial peptide gene expression},

author = {Jean-Luc Dimarcq and Jean-Luc Imler and R Lanot and Alan R B Ezekowitz and Jules A Hoffmann and C A Janeway and Marie Lagueux},

issn = {0965-1748},

year  = {1997},

date = {1997-10-01},

journal = {Insect Biochemistry and Molecular Biology},

volume = {27},

number = {10},

pages = {877--886},

abstract = {Insects rely on both humoral and cellular mechanisms to defend themselves against microbial infections. The humoral response involves synthesis of a battery of potent antimicrobial peptides by the fat body and, to a lesser extent, by blood cells. The cellular response on the other hand consists of phagocytosis of small microorganisms and melanization and encapsulation of larger parasites. The l(2)mbn cell line, established from tumorous larval hemocytes, represents a system of choice to dissect the molecular events controlling cellular immunity. We report here that l(2)mbn cells can be efficiently induced to differentiate in adherent, macrophage-like cells by treatment with 20-hydroxyecdysone. Ecdysone treatment increases both the phagocytic capacity of l(2)mbn cells and their competence to express antimicrobial genes in response to immune challenge. We also report that expression of several regulatory molecules thought to be involved in the immune response is up-regulated by ecdysone in l(2)mbn cells.},

keywords = {Animals, Bacterial Infections, Cellular, Ecdysone, Gene Expression, Genes, Hemocytes, Hemolymph, hoffmann, imler, Immunity, Insect, M3i, Macrophages, Peptide Biosynthesis, Phagocytosis},

pubstate = {published},

tppubtype = {article}

}

@article{hoffmann_chinless_1991,

title = {Chinless wonders. Molecular Aspects of Invertebrate Hormones. A Jacques Monod Conference sponsored by the Centre National de la Recherche Scientifique, Roscoff, France, October 14-19, 1990},

author = {Jules A Hoffmann and J Joosse},

issn = {1043-4674},

year  = {1991},

date = {1991-04-01},

journal = {New Biol.},

volume = {3},

number = {4},

pages = {336--340},

keywords = {Animals, Crustacea, Ecdysone, hoffmann, insects, Invertebrate Hormones, Juvenile Hormones, M3i, Neuropeptides, Receptors, Snails, Steroid},

pubstate = {published},

tppubtype = {article}

}

@article{kappler_characterization_1988,

title = {Characterization of three hydroxylases involved in the final steps of biosynthesis of the steroid hormone ecdysone in Locusta migratoria (Insecta, Orthoptera)},

author = {Christine Kappler and M Kabbouh and Charles Hetru and F Durst and Jules A Hoffmann},

issn = {0022-4731},

year  = {1988},

date = {1988-12-01},

journal = {J. Steroid Biochem.},

volume = {31},

number = {6},

pages = {891--898},

abstract = {It is most generally accepted that the last three enzymatic reactions in the biosynthetic pathway of ecdysone are, in this order, the hydroxylations at positions C-25, C-22 and C-2. Using high specific activity tritiated ecdysone precursors (2,22,25-trideoxyecdysone, 2,22-dideoxyecdysone and 2-deoxyecdysone) we have characterized the hydroxylases involved in these reactions, in the major biosynthetic tissue of ecdysone, i.e. the prothoracic glands. We show that C-2 hydroxylase is a mitochondrial oxygenase which differs from conventional cytochrome P-450-dependent monooxygenases by its relative insensitivity to CO. In contrast, C-22 and C-25 hydroxylases appear as classical cytochrome P-450 monooxygenases; C-22 hydroxylase is a mitochondrial enzyme whereas our data point to a microsomal localization of the C-25 hydroxylase.},

keywords = {Animals, Biological, Ecdysone, Grasshoppers, hoffmann, Kinetics, M3i, Mixed Function Oxygenases, Models, NAD, NADP, Subcellular Fractions},

pubstate = {published},

tppubtype = {article}

}

@article{lanot_further_1988,

title = {Further experimental evidence for the involvement of ecdysone in the control of meiotic reinitiation in oocytes of Locusta migratoria (Insecta, Orthoptera)},

author = {R Lanot and J Thiebold and M F Costet-Corio and P Benveniste and Jules A Hoffmann},

issn = {0012-1606},

year  = {1988},

date = {1988-03-01},

journal = {Dev. Biol.},

volume = {126},

number = {1},

pages = {212--214},

abstract = {Ecdysone has recently been shown to be able to trigger meiotic reinitiation in vitro in submature oocytes of Locusta. In the present study we have experimentally depressed (by 60-70%) ecdysone biosynthesis in the ovaries of adult females by rearing them on a diet with a modified sterol profile. Mature oocytes from such females fail to undergo normal reinitiation, but when incubated in vitro, can be induced to break their meiotic arrest by the addition of exogenous ecdysone. These results lend further support to the hypothesis that in Locusta, ovarian ecdysone is involved in the control of meiotic reinitiation.},

keywords = {Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Meiosis, Oocytes, Ovary},

pubstate = {published},

tppubtype = {article}

}

@article{meister_conversion_1985,

title = {Conversion of a radiolabelled ecdysone precursor, 2,22,25-trideoxyecdysone, by embryonic and larval tissues of Locusta migratoria},

author = {Marie F Meister and Jean-Luc Dimarcq and Christine Kappler and Charles Hetru and Marie Lagueux and R Lanot and B Luu and Jules A Hoffmann},

issn = {0303-7207},

year  = {1985},

date = {1985-06-01},

journal = {Mol. Cell. Endocrinol.},

volume = {41},

number = {1},

pages = {27--44},

abstract = {A high specific activity tritiated ecdysone precursor, 2,22,25-trideoxyecdysone, was used to probe the capacity of various embryonic and larval tissues to perform the last 3 hydroxylation steps in ecdysone biosynthesis. Embryos at early stages of development, prior to the differentiation of their endocrine glands and embryonic heads, thoraces and abdomens of later stages, were found to have the capacity to hydroxylate the precursor to ecdysone. Larval epidermis and fat body are also able to transform 2,22,25-trideoxyecdysone into ecdysone; Malpighian tubules and midgut hydroxylate the precursor at C-2 but are apparently unable to hydroxylate both at C-22 and C-25. Larval prothoracic glands convert the precursor to ecdysone at a very efficient rate, which is 1-2 magnitudes higher than that of the other tissues investigated; several data argue for the existence of a privileged sequence of hydroxylations, C-25, C-22, C-2, in the larval prothoracic glands.},

keywords = {Abdomen, Animals, Cholestenones, Chromatography, Ecdysone, Epidermis, Fat Body, Grasshoppers, Head, High Pressure Liquid, hoffmann, Hydroxylation, Larva, M3i, Malpighian Tubules, Thorax},

pubstate = {published},

tppubtype = {article}

}

@article{hetru_ecdysone_1985,

title = {Ecdysone conjugates: isolation and identification},

author = {Charles Hetru and B Luu and Jules A Hoffmann},

issn = {0076-6879},

year  = {1985},

date = {1985-01-01},

journal = {Meth. Enzymol.},

volume = {111},

pages = {411--419},

keywords = {Animals, Bombyx, Chromatography, Ecdysone, Helix (Snails), High Pressure Liquid, hoffmann, Insect Hormones, M3i, Magnetic Resonance Spectroscopy, Mass Spectrometry, Spectrophotometry, Structure-Activity Relationship},

pubstate = {published},

tppubtype = {article}

}

@article{tsoupras_isolation_1982,

title = {Isolation and identification of three ecdysteroid conjugates with a C-20 hydroxy group in eggs of Locusta migratoria},

author = {G Tsoupras and B Luu and Jules A Hoffmann},

issn = {0039-128X},

year  = {1982},

date = {1982-11-01},

journal = {Steroids},

volume = {40},

number = {5},

pages = {551--560},

abstract = {Three ecdysteroids conjugates with a hydroxy group at C-20 were isolated from developing eggs of locusta migratoria and identified as 22-phosphate conjugates of 2-deoxy-20-hydroxy-ecdysone, 20-hydroxyecdysone and 20-hydroxyecdysone acetate.},

keywords = {Animals, Chromatography, Ecdysone, Ecdysterone, Female, Grasshoppers, High Pressure Liquid, hoffmann, M3i, Magnetic Resonance Spectroscopy, Ovum, Phosphates, Spectrophotometry, Ultraviolet},

pubstate = {published},

tppubtype = {article}

}

@article{lachaise_ecdysteroids_1982,

title = {Ecdysteroids and embryonic development in the shore crab, Carcinus maenas},

author = {F Lachaise and Jules A Hoffmann},

issn = {0018-4888},

year  = {1982},

date = {1982-09-01},

journal = {Hoppe-Seyler's Z. Physiol. Chem.},

volume = {363},

number = {9},

pages = {1059--1067},

abstract = {Eggs at various stages of embryonic development of Carcinus maenas contain high concentrations of the ecdysteroid ponasterone A together with lower titres of 20-hydroxyecdysone and ecdysone. Correlative studies on ecdysteroid titres and developmental characteristics of Carcinus embryos indicate that one function of ponasterone A might be related to the control of deposition of an embryonic envelope.},

keywords = {Animals, Brachyura, Ecdysone, Ecdysterone, Embryo, Female, hoffmann, M3i, Nonmammalian, Ovum, Radioimmunoassay},

pubstate = {published},

tppubtype = {article}

}

@article{hetru_biosynthetic_1982,

title = {The biosynthetic pathway of ecdysone: studies with vitellogenic ovaries of Locusta migratoria (Orthoptera)},

author = {Charles Hetru and Christine Kappler and Jules A Hoffmann and R Nearn and Lee Bang and D H Horn},

issn = {0303-7207},

year  = {1982},

date = {1982-04-01},

journal = {Mol. Cell. Endocrinol.},

volume = {26},

number = {1-2},

pages = {51--80},

abstract = {Ovaries of adult females of Locusta migratoria synthesize impressive amounts of the steroid hormone ecdysone (and related ecdysteroids) during the late phases of vitellogenesis. The present study, aimed at elucidating the sequence of the biosynthetic steps that lead from cholesterol to ecdysone, has taken benefit of this remarkable biological model by using a double approach: (1) isolation and physico-chemical identification of endogenous biogenetic intermediates; (2) metabolic study of labelled putative precursor molecules. The data presented in this paper lead us to propose the following sequence of events: conversion of cholesterol to 3 beta-hydroxy-5 beta-cholest-7-en-6-one (via several intermediates not identified in this study) followed by 14 beta-hydroxylation to 3 beta, 14 alpha-dihydroxy-5 beta-cholest-7-en-6-one; hydroxylation on the side-chain at C-25 and C-22 (in this order) to 2-deoxyecdysone; hydroxylation at C-2 to ecdysone.},

keywords = {Animals, Chromatography, Ecdysone, Female, Gas Chromatography-Mass Spectrometry, High Pressure Liquid, hoffmann, M3i, Orthoptera, Ovary},

pubstate = {published},

tppubtype = {article}

}

@article{lachaise_ecdysteroids_1981,

title = {Ecdysteroids and ovarian development in the shore crab, Carcinus maenas},

author = {F Lachaise and M Goudeau and Charles Hetru and Christine Kappler and Jules A Hoffmann},

issn = {0018-4888},

year  = {1981},

date = {1981-01-01},

journal = {Hoppe-Seyler's Z. Physiol. Chem.},

volume = {362},

number = {5},

pages = {521--529},

abstract = {Mature ovaries of the shore crab Carcinus maenas contain large concentrations of three major ecdysteroids which we have identified by physicochemical methods as ecdysone, 20-hydroxyecdysone and ponasterone A. The fluctuations of ovarian and blood ecdysteroid concentrations are presented in relation to the various stages of ovarian development.},

keywords = {aging, Animals, Brachyura, Cross Reactions, Ecdysone, Ecdysterone, Female, hoffmann, M3i, Ovary, Radioimmunoassay, Sexual Maturation},

pubstate = {published},

tppubtype = {article}

}

@article{zachary_endocrine_1980,

title = {Endocrine control of the metamorphosis of the larval muscles in Calliphora erythrocephala (Diptera): in vitro studies of the role of ecdysteroids},

author = {Daniel Zachary and Jules A Hoffmann},

issn = {0012-1606},

year  = {1980},

date = {1980-11-01},

journal = {Dev. Biol.},

volume = {80},

number = {1},

pages = {235--247},

keywords = {Animals, Biological, Diptera, Ecdysone, Ecdysterone, hoffmann, Larva, M3i, Metamorphosis, Muscles, Organ Culture Techniques},

pubstate = {published},

tppubtype = {article}

}

@article{goltzene_follicle_1978,

title = {The follicle cell epithelium of maturing ovaries of Locusta migratoria: a new biosynthetic tissue for ecdysone},

author = {F Goltzené and Marie Lagueux and Maurice Charlet and Jules A Hoffmann},

issn = {0018-4888},

year  = {1978},

date = {1978-10-01},

journal = {Hoppe-Seyler's Z. Physiol. Chem.},

volume = {359},

number = {10},

pages = {1427--1434},

abstract = {Follicle cells of maturing ovaries of Locusta migratoria are demonstrated to synthesize the moulting hormone ecdysone (2beta,3beta,14alpha,22R,25-pentahydroxy-5beta-cholest-7-en-6-one). Studies of secretory kinetics under in vitro conditions show that the intensity of hormone secretion is strictly dependent on the stage of maturation of the excised ovaries.},

keywords = {Animals, Ecdysone, Epithelium, Female, Grasshoppers, hoffmann, Kinetics, M3i, Oocytes, Ovarian Follicle, Ovary, Sexual Maturation},

pubstate = {published},

tppubtype = {article}

}

@article{hetru_adult_1978,

title = {Adult ovaries of Locusta migratoria contain the sequence of biosynthetic intermediates for ecdysone},

author = {Charles Hetru and Marie Lagueux and L Bang and Jules A Hoffmann},

issn = {0024-3205},

year  = {1978},

date = {1978-06-01},

journal = {Life Sci.},

volume = {22},

number = {23},

pages = {2141--2154},

keywords = {Animals, Chemical Phenomena, Chemistry, Cholesterol, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary, Ovum},

pubstate = {published},

tppubtype = {article}

}

@article{lagueux_ecdysone_1977,

title = {Ecdysone during ovarian development in Locusta migratoria},

author = {Marie Lagueux and M Hirn and Jules A Hoffmann},

issn = {0022-1910},

year  = {1977},

date = {1977-01-01},

journal = {J. Insect Physiol.},

volume = {23},

number = {1},

pages = {109--119},

keywords = {Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Male, Oogenesis, Ovary},

pubstate = {published},

tppubtype = {article}

}

@article{bang_identification_1976,

title = {Identification, by gas chromatography and mass spectrometry, of ecdysone synthesized in the ovaries of adult female Locusta migratoria (Insecta, Orthoptera)},

author = {L Bang and Marie Lagueux and M Hirn and Jules A Hoffmann},

year  = {1976},

date = {1976-10-01},

journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},

volume = {283},

number = {9},

pages = {1081--1084},

abstract = {Gas chromatographic analysis, coupled to mass spectrometry, demonstrates that the considerable amounts of ecdysteroids produced in the ovaries of female adults of Locusta migratoria at the end of each ovarian cycle, consist predominantly of ecdysone.},

keywords = {Age Factors, Animals, Chromatography, Ecdysone, Female, Gas, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary},

pubstate = {published},

tppubtype = {article}

}

@article{feyereisen_dynamics_1976,

title = {Dynamics of ecdysone metabolism after ingestion and injection in Locusta migratoria},

author = {R Feyereisen and Marie Lagueux and Jules A Hoffmann},

issn = {0016-6480},

year  = {1976},

date = {1976-07-01},

journal = {Gen. Comp. Endocrinol.},

volume = {29},

number = {3},

pages = {319--327},

keywords = {Animals, Ecdysone, Grasshoppers, hoffmann, Hydroxylation, Injections, Intestines, Larva, M3i, Malpighian Tubules, Oral},

pubstate = {published},

tppubtype = {article}

}

@article{feyereisen_hemolymphatic_1975,

title = {The hemolymphatic transport of molting hormone during the development of Locusta migratoria L},

author = {R Feyereisen and Marie Lagueux and Jules A Hoffmann},

year  = {1975},

date = {1975-04-01},

journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},

volume = {280},

number = {14},

pages = {1709--1712},

abstract = {Shortly after injection of radio-labelled ecdysone into fifth instar larvae of Locusta migratoria, 20-hydroxy-ecdysone (ecdysterone) is the main hormone found in the blood. Some 10% of the circulating hormone are bound to hemolymph macromolecules. The ratio of bound to free hormone is stage-dependent; it decreases considerably after previous injections of non-labelled ecdysone, but increases in insects in which ecdysone biosynthesis has been blocked by extirpation of the prothoracic glands or selective X-ray treatment of the hemocytopoietic tissue.},

keywords = {Age Factors, Animals, Carrier Proteins, Chromatography, Ecdysone, Ecdysterone, Gel, Grasshoppers, Hematopoietic System, hoffmann, Larva, M3i, Neurosecretory Systems, Protein Binding, Time Factors},

pubstate = {published},

tppubtype = {article}

}