Publications
2000
Postawski K., Olech-Fudali E., Jakowicki J. A., Korobowicz E., Keith G., Baranowski W.
[Overall genomic DNA methylation in relation to estrogen] Journal Article
In: Ginekol Pol, vol. 71, no. 9, pp. 1206-11, 2000, (0017-0011 Journal Article).
Abstract | BibTeX | Tags: 80, Abstract, Aged, and, Biopsy, DNA, English, Estrogen/*metabolism, Female, Gov't, Human, Methylases/metabolism, Methylation, Middle, modification, Neoplasms/*metabolism/*pathology, Non-U.S., over, Progesterone/*metabolism, Receptors, Support, Uterine, Uterus/*metabolism/pathology
@article{,
title = {[Overall genomic DNA methylation in relation to estrogen]},
author = { K. Postawski and E. Olech-Fudali and J. A. Jakowicki and E. Korobowicz and G. Keith and W. Baranowski},
year = {2000},
date = {2000-01-01},
journal = {Ginekol Pol},
volume = {71},
number = {9},
pages = {1206-11},
abstract = {Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors. Additionally, there was no low DNA methylation in ER negative uterine cancer tissues. Decrease of the overall genomic DNA methylation could be related to the increase of ER/PR ratio, however it was not significant in our investigation. The potential role of steroid receptors status in uterine cancer tissue is discussed.},
note = {0017-0011
Journal Article},
keywords = {80, Abstract, Aged, and, Biopsy, DNA, English, Estrogen/*metabolism, Female, Gov't, Human, Methylases/metabolism, Methylation, Middle, modification, Neoplasms/*metabolism/*pathology, Non-U.S., over, Progesterone/*metabolism, Receptors, Support, Uterine, Uterus/*metabolism/pathology},
pubstate = {published},
tppubtype = {article}
}
Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors. Additionally, there was no low DNA methylation in ER negative uterine cancer tissues. Decrease of the overall genomic DNA methylation could be related to the increase of ER/PR ratio, however it was not significant in our investigation. The potential role of steroid receptors status in uterine cancer tissue is discussed.