Publications
2001
Postawski K., Olech-Fudali E., Korobowicz E., Jakowicki J. A., Keith G., Baranowski W.
[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer.] Journal Article
In: Ginekol Pol, vol. 72, no. 9, pp. 709-16, 2001, (0017-0011 Journal Article).
Abstract | BibTeX | Tags: Abstract, Adducts/*analysis, Autoradiography, DNA, English, Estrogen/*analysis, Female, Human, Neoplasm/*analysis, Neoplasms/genetics/*pathology, Progesterone/*analysis, Receptors, Uterine
@article{,
title = {[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer.]},
author = { K. Postawski and E. Olech-Fudali and E. Korobowicz and J. A. Jakowicki and G. Keith and W. Baranowski},
year = {2001},
date = {2001-01-01},
journal = {Ginekol Pol},
volume = {72},
number = {9},
pages = {709-16},
abstract = {OBJECTIVE: Determination of the relationship between hydrophobic DNA adducts (A) and estrogen receptors (ER) and progesterone (PR) receptor status in uterine cancers. METHODS: Using the P1 enriched version of 32P-postlabeling for hydrophobic DNA adducts detection on polyethyleneimine (PEI) cellulose thin layer chromatograms (TLC) we examined 11 uterine cancer DNAs. The quantification of the adducts was performed by Cerenkov counting of the spots. ER and PR status was recognized histochemically and H-score estimate was performed for each investigated cancer tissue. Patterns of uterine cancer DNA adducts were compared to the maps of adducts recognized in normal human endometrium. RESULTS: In three of the studied uterine cancers there was no positive staining of ER and PR; in one case there was a weak ER staining but PR staining was negative. In ER negative tumors the A level was significantly higher than in ER positive cancers (138.1 +/- 64.1 vs. 49.7 +/- 26.8 adducts per 10(9) nucleotides, respectively, p < 0.05). Highest A levels were found in two ER and PR negative G3 metastatic tumors. Finally, in all investigated cancers there was a strong, inverse correlation between ER content and A level (r = -0.67, p < 0.03). In addition, the correlation between PR level and A was of borderline significance (r = -0.6},
note = {0017-0011
Journal Article},
keywords = {Abstract, Adducts/*analysis, Autoradiography, DNA, English, Estrogen/*analysis, Female, Human, Neoplasm/*analysis, Neoplasms/genetics/*pathology, Progesterone/*analysis, Receptors, Uterine},
pubstate = {published},
tppubtype = {article}
}
1995
Keith G., Dirheimer G.
Postlabeling: a sensitive method for studying DNA adducts and their role in carcinogenesis Journal Article
In: Curr Opin Biotechnol, vol. 6, no. 1, pp. 3-11, 1995, (0958-1669 Journal Article Review Review, Academic).
Abstract | BibTeX | Tags: *Cell, *Genome, Adducts/*analysis, and, Animals, Base, Cell, Conditions/genetics/pathology, Data, Dilution, Division, DNA, Genetic, Gov't, Human, Models, Molecular, Mutagenesis, Neoplasms/*genetics/pathology, Neoplastic, Non-U.S., Phosphorus, Precancerous, Radioisotope, Radioisotopes, Sensitivity, Sequence, Specificity, Support, Technique, Transformation, Xenobiotics
@article{,
title = {Postlabeling: a sensitive method for studying DNA adducts and their role in carcinogenesis},
author = { G. Keith and G. Dirheimer},
year = {1995},
date = {1995-01-01},
journal = {Curr Opin Biotechnol},
volume = {6},
number = {1},
pages = {3-11},
abstract = {The covalent binding of xenobiotics to DNA is an important trigger of the multistage process that leads to carcinogenesis. 32P-postlabeling represents a highly sensitive method for biomonitoring exposure to genotoxic agents and for cancer risk assessment; it is capable of detecting less than one DNA adduct per human genome. Recent improvements to the technique have shown that the resistance of adducted DNA to enzyme digestion may lead to an overestimation of the number of different adducts present in a sample.},
note = {0958-1669
Journal Article
Review
Review, Academic},
keywords = {*Cell, *Genome, Adducts/*analysis, and, Animals, Base, Cell, Conditions/genetics/pathology, Data, Dilution, Division, DNA, Genetic, Gov't, Human, Models, Molecular, Mutagenesis, Neoplasms/*genetics/pathology, Neoplastic, Non-U.S., Phosphorus, Precancerous, Radioisotope, Radioisotopes, Sensitivity, Sequence, Specificity, Support, Technique, Transformation, Xenobiotics},
pubstate = {published},
tppubtype = {article}
}