Our group is interested in the localization of post-transcriptional modifications of RNA by mass spectrometry. We focus particularly on mapping modifications in transfer RNAs, using a bottom up strategy. The tRNAs are separated by two-dimensional electrophoresis and then individually digested by a RNase (e.g. RNase T1) in order to be sequenced by Lc MS/MS.
Instrumentation : Synapt G2 (Waters) + nanoAquity (Waters)