M3i

@article{hemmerlin_profiling_2014,

title = {Profiling of defense responses in Escherichia coli treated with fosmidomycin.},

author = {Andréa Hemmerlin and Denis Tritsch and Philippe Hammann and Michel Rohmer and Thomas J Bach},

doi = {10.1016/j.biochi.2013.11.008},

issn = {1638-6183 0300-9084},

year  = {2014},

date = {2014-01-01},

journal = {Biochimie},

volume = {99},

pages = {54--62},

abstract = {The mevalonate-independent isoprenoid biosynthesis pathway has been recognized as a promising target for designing new antibiotics. But pathogens treated with compounds such as fosmidomycin, a slow binding inhibitor of 1-deoxy-D-xylulose 5-phosphate reducto-isomerase, the second enzyme in this pathway, develop rapid drug resistance. In Escherichia coli, acquired resistance results mostly from inactivating the cAMP-dependent glpT transporter, thereby preventing import of the inhibitor. Such mutant strains are characterized by cross-resistance to fosfomycin, by susceptibility to efflux pump inhibitors, by disability to use glycerol 3-phosphate as a carbon source or by increased activity of the promoter controlling the expression of the glpABC regulon when grown in presence of fosmidomycin. The quite challenging task consists in conceiving new and efficient inhibitors avoiding resistance acquisition. They should be efficient in blocking the target enzyme, but should also be durably taken up by the organism. To address this issue, it is essential to characterize the mechanisms the pathogen exploits to defeat the antibiotic before resistance is acquired. Having this in mind, a 2-D Fluorescence Difference Gel Electrophoresis proteomic approach has been applied to identify defense responses in E. coli cells being shortly exposed to fosmidomycin (3 h). It seems that combined strategies are promptly induced. The major one consists in preventing toxic effects of the compound either by adapting metabolism and/or by getting rid of the molecule. The strategy adopted by the bacteria is to eliminate the drug from the cell or to increase the tolerance to oxidative stress. The design of new, but still efficient drugs, needs consideration of such rapid modulations required to adapt cell growth in contact of the inhibitor.},

note = {Place: France},

keywords = {1-Deoxy-d-xylulose 5-phosphate reducto-isomerase, Anti-Bacterial Agents/*pharmacology, Antibiotics, Bacterial, Disk Diffusion Antimicrobial Tests, Drug Resistance, Escherichia coli Proteins/*metabolism, Escherichia coli/drug effects/growth & development/*metabolism, Fosfomycin/*analogs & derivatives/pharmacology, Fosmidomycin, Isoprenoid, Oxidative Stress, Phenotype, PPSE, Proteome/metabolism, Resistance acquisition},

pubstate = {published},

tppubtype = {article}

}

@article{russier_evidencing_2013,

title = {Evidencing the mask effect of graphene oxide: a comparative study on primary human and murine phagocytic cells},

author = {Julie Russier and Emanuele Treossi and Alessia Scarsi and Francesco Perrozzi and Hélène Dumortier and Luca Ottaviano and Moreno Meneghetti and Vincenzo Palermo and Alberto Bianco},

doi = {10.1039/c3nr03543c},

issn = {2040-3372},

year  = {2013},

date = {2013-01-01},

journal = {Nanoscale},

volume = {5},

number = {22},

pages = {11234--11247},

abstract = {Graphene oxide (GO) is attracting an ever-growing interest in different fields and applications. Not much is known about the possible impact of GO sheet lateral dimensions on their effects in vitro, especially on human primary cells. In an attempt to address this issue, we present a study to evaluate, how highly soluble 2-dimensional GO constituted of large or small flakes affects human monocyte derived macrophages (hMDM). For this purpose, the lateral size of GO was tuned using sonication and three samples were obtained. The non sonicated one presented large flakes (textasciitilde1.32 μm) while sonication for 2 and 26 hours generated small (textasciitilde0.27 μm) and very small (textasciitilde0.13 μm) sheets of GO, respectively. Cell studies were then conducted to evaluate the cytotoxicity, the oxidative stress induction, the activation potential and the pro-inflammatory effects of these different types of GO at increasing concentrations. In comparison, the same experiments were run on murine intraperitoneal macrophages (mIPM). The interaction between GO and cells was further examined by TEM and Raman spectroscopy. Our data revealed that the GO sheet size had a significant impact on different cellular parameters (i.e. cellular viability, ROS generation, and cellular activation). Indeed, the more the lateral dimensions of GO were reduced, the higher were the cellular internalization and the effects on cellular functionality. Our data also revealed a particular interaction of GO flakes with the cellular membrane. In fact, a GO mask due to the parallel arrangement of the graphene sheets on the cellular surface was observed. Considering the mask effect, we have hypothesized that this particular contact between GO sheets and the cell membrane could either promote their internalization or isolate cells from their environment, thus possibly accounting for the following impact on cellular parameters.},

keywords = {Animals, Cell Survival, Cells, Cultured, Cytokines, Dumortier, Graphite, Humans, I2CT, Macrophages, Mice, Monocytes, Oxidative Stress, Oxides, Reactive Oxygen Species, Team-Bianco, Team-Dumortier},

pubstate = {published},

tppubtype = {article}

}

@article{bianco_fullerene-based_2001,

title = {Fullerene-based amino acids and peptides},

author = {A Bianco and T Da Ros and M Prato and C Toniolo},

doi = {10.1002/psc.313},

issn = {1075-2617},

year  = {2001},

date = {2001-04-01},

journal = {Journal of Peptide Science: An Official Publication of the European Peptide Society},

volume = {7},

number = {4},

pages = {208--219},

abstract = {Recent advances in the chemistry of fullerene have allowed the synthesis of many classes of novel fullerene derivatives. Among these classes, fullerene-based amino acids and peptides are particularly interesting, both for structural studies and biological applications. In this review, we will discuss our own achievements in this rapidly growing field. In particular, the application of fulleroproline (Fpr) amino acids and peptides to medicinal chemistry and material science will be highlighted.},

keywords = {Amino Acids, Animals, Antiviral Agents, carbon, Fullerenes, Humans, I2CT, Infections, Oxidative Stress, Peptides, Proline, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}