Issa Najwa, Guillaumot Nina, Lauret Emilie, Matt Nicolas, Scaeffer-Reiss Christine, Dorsselaer Alain Van, Reichhart Jean-Marc, Veillard Florian
The circulating protease Persephone is an immune sensor for microbial proteolytic activities upstream of the Drosophila Toll pathway. Journal Article
In: Molecular Cell, vol. 69, no. 4, pp. 539-550, 2018, ISSN: 1097-2765.
Abstract | Links | BibTeX | Tags: circulating protease, immune sensor, M3i, matt, persephone, Protease, proteolitic activities, reichhart, toll pathway
@article{Issa2018,
title = {The circulating protease Persephone is an immune sensor for microbial proteolytic activities upstream of the Drosophila Toll pathway.},
author = {Najwa Issa and Nina Guillaumot and Emilie Lauret and Nicolas Matt and Christine Scaeffer-Reiss and Alain Van Dorsselaer and Jean-Marc Reichhart and Florian Veillard},
url = {http://www.cell.com/molecular-cell/fulltext/S1097-2765(18)30058-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1097276518300583%3Fshowall%3Dtrue},
doi = {10.1016/j.molcel.2018.01.029},
issn = {1097-2765},
year = {2018},
date = {2018-02-15},
journal = {Molecular Cell},
volume = {69},
number = {4},
pages = {539-550},
abstract = {Microbial or endogenous molecular patterns as well as pathogen functional features can activate innate immune systems. Whereas detection of infection by pattern recognition receptors has been investigated in details, sensing of virulence factors activities remains less characterized. In Drosophila, genetic evidences indicate that the serine protease Persephone belongs to a danger pathway activated by abnormal proteolytic activities to induce Toll signaling. However, neither the activation mechanism of this pathway nor its specificity has been determined. Here, we identify a unique region in the pro-domain of Persephone that functions as bait for exogenous proteases independently of their origin, type, or specificity. Cleavage in this bait region constitutes the first step of a sequential activation and licenses the subsequent maturation of Persephone to the endogenous cysteine cathepsin 26-29-p. Our results establish Persephone itself as an immune receptor able to sense a broad range of microbes through virulence factor activities rather than molecular patterns.},
keywords = {circulating protease, immune sensor, M3i, matt, persephone, Protease, proteolitic activities, reichhart, toll pathway},
pubstate = {published},
tppubtype = {article}
}
Patrnogic Jelena, Leclerc Vincent
The serine protease homolog spheroide is involved in sensing of pathogenic Gram-positive bacteria Journal Article
In: PLoS One, vol. 12, no. 12, 2017.
Abstract | Links | BibTeX | Tags: Fungi, Gram-Positive Bacteria, serine protease, spheroide, toll pathway
@article{Jelena2017,
title = {The serine protease homolog spheroide is involved in sensing of pathogenic Gram-positive bacteria},
author = {Jelena Patrnogic and Vincent Leclerc},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718610/},
doi = {10.1371/journal.pone.0188339},
year = {2017},
date = {2017-12-06},
journal = {PLoS One},
volume = {12},
number = {12},
abstract = {In Drosophila, recognition of pathogens such as Gram-positive bacteria and fungi triggers the activation of proteolytic cascades and the subsequent activation of the Toll pathway. This response can be achieved by either detection of pathogen associated molecular patterns or by sensing microbial proteolytic activities ("danger signals"). Previous data suggested that certain serine protease homologs (serine protease folds that lack an active catalytic triad) could be involved in the pathway. We generated a null mutant of the serine protease homolog spheroide (sphe). These mutant flies are susceptible to Enterococcus faecalis infection and unable to fully activate the Toll pathway. Sphe is required to activate the Toll pathway after challenge with pathogenic Gram-Positive bacteria. Sphe functions in the danger signal pathway, downstream or at the level of Persephone.},
keywords = {Fungi, Gram-Positive Bacteria, serine protease, spheroide, toll pathway},
pubstate = {published},
tppubtype = {article}
}