Schaeffer Evelyne, Dehuyser Laure, Sigwalt David, Flacher Vincent, Bernacchi Serena, Chaloin Olivier, Remy Jean-Serge, Mueller Christopher G, Baati Rachid, Wagner Alain
Dynamic micelles of mannoside glycolipids are more efficient than polymers for inhibiting HIV-1 trans-infection Journal Article
In: Bioconjugate Chemistry, vol. 24, no. 11, pp. 1813–1823, 2013, ISSN: 1520-4812.
Abstract | Links | BibTeX | Tags: Anti-HIV Agents, Calcium, Cells, Chemistry, Cultured, Dendritic Cells, Dose-Response Relationship, Drug, Electron, fluorescence, Glycolipids, HIV, HIV Infections, HIV-1, Human, Humans, immunodeficiency, immunopathology, inhibition, LECTIN, Lectins, lipid, Mannosides, Micelles, Microbial Sensitivity Tests, Microscopy, Models, Molecular, Molecular Structure, Polymers, prophylaxis, Spectrometry, Structure-Activity Relationship, Surface Plasmon Resonance, target, Team-Mueller, Thermodynamics, Transmission, virus
@article{schaeffer_dynamic_2013,
title = {Dynamic micelles of mannoside glycolipids are more efficient than polymers for inhibiting HIV-1 trans-infection},
author = {Evelyne Schaeffer and Laure Dehuyser and David Sigwalt and Vincent Flacher and Serena Bernacchi and Olivier Chaloin and Jean-Serge Remy and Christopher G Mueller and Rachid Baati and Alain Wagner},
doi = {10.1021/bc4000806},
issn = {1520-4812},
year = {2013},
date = {2013-11-01},
journal = {Bioconjugate Chemistry},
volume = {24},
number = {11},
pages = {1813--1823},
abstract = {Mannoside glycolipid conjugates are able to inhibit human immunodeficiency virus type 1 (HIV-1) trans-infection mediated by human dendritic cells (DCs). The conjugates are formed by three building blocks: a linear or branched mannose head, a hydrophilic linker, and a 24-carbon lipid chain. We have shown that, even as single molecules, these compounds efficiently target mannose-binding lectins, such as DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN) important for HIV-1 transmission. With the goal to optimize their inhibitory activity by supramolecular structure formation, we have compared saturated and unsaturated conjugates, as single molecules, self-assemblies of dynamic micelles, and photopolymerized cross-linked polymers. Surface plasmon resonance showed that, unexpectedly, polymers of trivalent conjugates did not display a higher binding affinity for DC-SIGN than single molecules. Interactions on a chip or in solution were independent of calcium; however, binding to DCs was inhibited by a calcium chelator. Moreover, HIV-1 trans-infection was mostly inhibited by dynamic micelles and not by rigid polymers. The inhibition data revealed a clear correlation between the structure and molecular assembly of a conjugate and its biological antiviral activity. We present an interaction model between DC-SIGN and conjugates-either single molecules, micelles, or polymers-that highlights that the most effective interactions by dynamic micelles involve both mannose heads and lipid chains. Our data reveal that trivalent glycolipid conjugates display the highest microbicide potential for HIV prophylaxis, as dynamic micelles conjugates and not as rigid polymers.},
keywords = {Anti-HIV Agents, Calcium, Cells, Chemistry, Cultured, Dendritic Cells, Dose-Response Relationship, Drug, Electron, fluorescence, Glycolipids, HIV, HIV Infections, HIV-1, Human, Humans, immunodeficiency, immunopathology, inhibition, LECTIN, Lectins, lipid, Mannosides, Micelles, Microbial Sensitivity Tests, Microscopy, Models, Molecular, Molecular Structure, Polymers, prophylaxis, Spectrometry, Structure-Activity Relationship, Surface Plasmon Resonance, target, Team-Mueller, Thermodynamics, Transmission, virus},
pubstate = {published},
tppubtype = {article}
}
Al-Jamal Khuloud T, Gherardini Lisa, Bardi Giuseppe, Nunes Antonio, Guo Chang, Bussy Cyrill, Herrero Antonia M, Bianco Alberto, Prato Maurizio, Kostarelos Kostas, Pizzorusso Tommaso
Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing Journal Article
In: Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 27, pp. 10952–10957, 2011, ISSN: 1091-6490.
Abstract | Links | BibTeX | Tags: Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission
@article{al-jamal_functional_2011,
title = {Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing},
author = {Khuloud T Al-Jamal and Lisa Gherardini and Giuseppe Bardi and Antonio Nunes and Chang Guo and Cyrill Bussy and Antonia M Herrero and Alberto Bianco and Maurizio Prato and Kostas Kostarelos and Tommaso Pizzorusso},
doi = {10.1073/pnas.1100930108},
issn = {1091-6490},
year = {2011},
date = {2011-07-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {108},
number = {27},
pages = {10952--10957},
abstract = {Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inhibition of caspases. Here, we studied whether silencing of Caspase-3 using carbon nanotube-mediated in vivo RNA interference (RNAi) could offer a therapeutic opportunity against stroke. Effective delivery of siRNA directly to the CNS has been shown to normalize phenotypes in animal models of several neurological diseases. It is shown here that peri-lesional stereotactic administration of a Caspase-3 siRNA (siCas 3) delivered by functionalized carbon nanotubes (f-CNT) reduced neurodegeneration and promoted functional preservation before and after focal ischemic damage of the rodent motor cortex using an endothelin-1 induced stroke model. These observations illustrate the opportunity offered by carbon nanotube-mediated siRNA delivery and gene silencing of neuronal tissue applicable to a variety of different neuropathological conditions where intervention at well localized brain foci may offer therapeutic and functional benefits.},
keywords = {Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
den Bossche Jeroen Van, Al-Jamal Wafa' T, Tian Bowen, Nunes Antonio, Fabbro Chiara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes Journal Article
In: Chemical Communications (Cambridge, England), vol. 46, no. 39, pp. 7379–7381, 2010, ISSN: 1364-548X.
Abstract | Links | BibTeX | Tags: Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor
@article{van_den_bossche_efficient_2010,
title = {Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes},
author = {Jeroen Van den Bossche and Wafa' T Al-Jamal and Bowen Tian and Antonio Nunes and Chiara Fabbro and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1039/c0cc02092c},
issn = {1364-548X},
year = {2010},
date = {2010-10-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {46},
number = {39},
pages = {7379--7381},
abstract = {We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.},
keywords = {Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor},
pubstate = {published},
tppubtype = {article}
}
Lacerda Lara, Herrero Maria A, Venner Kerrie, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon-nanotube shape and individualization critical for renal excretion Journal Article
In: Small (Weinheim an Der Bergstrasse, Germany), vol. 4, no. 8, pp. 1130–1132, 2008, ISSN: 1613-6829.
Links | BibTeX | Tags: Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission
@article{lacerda_carbon-nanotube_2008,
title = {Carbon-nanotube shape and individualization critical for renal excretion},
author = {Lara Lacerda and Maria A Herrero and Kerrie Venner and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200800323},
issn = {1613-6829},
year = {2008},
date = {2008-08-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {4},
number = {8},
pages = {1130--1132},
keywords = {Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
Fabre Bruno, Hauquier Fanny, Herrier Cyril, Pastorin Giorgia, Wu Wei, Bianco Alberto, Prato Maurizio, Hapiot Philippe, Zigah Dodzi, Prasciolu Mauro, Vaccari Lisa
Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces Journal Article
In: Langmuir: the ACS journal of surfaces and colloids, vol. 24, no. 13, pp. 6595–6602, 2008, ISSN: 0743-7463.
Abstract | Links | BibTeX | Tags: Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco
@article{fabre_covalent_2008,
title = {Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces},
author = {Bruno Fabre and Fanny Hauquier and Cyril Herrier and Giorgia Pastorin and Wei Wu and Alberto Bianco and Maurizio Prato and Philippe Hapiot and Dodzi Zigah and Mauro Prasciolu and Lisa Vaccari},
doi = {10.1021/la800358w},
issn = {0743-7463},
year = {2008},
date = {2008-06-01},
journal = {Langmuir: the ACS journal of surfaces and colloids},
volume = {24},
number = {13},
pages = {6595--6602},
abstract = {Multiwalled carbon nanotubes (MWNTs) covalently bound to monocrystalline p-type Si(111) surfaces have been prepared by attaching soluble amine-functionalized MWNTs onto a preassembled undecanoic acid monolayer using carbodiimide coupling. SEM analysis of these functionalized surfaces shows that the bound MWNTs are parallel to the surface rather than perpendicular. The voltammetric and electrochemical impedance spectroscopy measurements reveal that the electron transfer at the MWNT-modified surface is faster than that observed at a MWNT-free alkyl monolayer. We have also demonstrated that it is possible to prepare MWNT micropatterns using this surface amidation reaction and a "reagentless" UV photolithography technique. Following this approach, MWNT patterns surrounded by n-dodecyl areas have been produced and the local electrochemical properties of these micropatterned surfaces have been examined by scanning electrochemical microscopy. In particular, it is demonstrated that the MWNT patterns allow a faster charge transfer which is consistent with the results obtained for the uniformly modified surfaces.},
keywords = {Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Opportunities and challenges of carbon-based nanomaterials for cancer therapy Journal Article
In: Expert Opinion on Drug Delivery, vol. 5, no. 3, pp. 331–342, 2008, ISSN: 1742-5247.
Abstract | Links | BibTeX | Tags: Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission
@article{bianco_opportunities_2008,
title = {Opportunities and challenges of carbon-based nanomaterials for cancer therapy},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1517/17425247.5.3.331},
issn = {1742-5247},
year = {2008},
date = {2008-01-01},
journal = {Expert Opinion on Drug Delivery},
volume = {5},
number = {3},
pages = {331--342},
abstract = {The possibility of incorporating carbon-based nanomaterials into living systems has opened the way for the investigation of their potential applications in the emerging field of nanomedicine. A wide variety of different nanomaterials based on allotropic forms of carbon, such as nanotubes, nanohorns and nanodiamonds, are currently being explored towards different biomedical applications. In this review, we discuss the recent advances in the development of these novel nanomaterials for cancer therapy. A comparison between the characteristics, the advantages, the drawbacks, the benefits and the risks associated with these novel biocompatible forms of carbon is presented here.},
keywords = {Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission},
pubstate = {published},
tppubtype = {article}
}
Ali-Boucetta Hanene, Al-Jamal Khuloud T, McCarthy David, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Multiwalled carbon nanotube-doxorubicin supramolecular complexes for cancer therapeutics Journal Article
In: Chemical Communications (Cambridge, England), no. 4, pp. 459–461, 2008, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: Antineoplastic Agents, Breast Neoplasms, carbon, Cultured, Doxorubicin, Electron, Humans, I2CT, Microscopy, Nanotubes, Team-Bianco, Transmission, Tumor Cells
@article{ali-boucetta_multiwalled_2008,
title = {Multiwalled carbon nanotube-doxorubicin supramolecular complexes for cancer therapeutics},
author = {Hanene Ali-Boucetta and Khuloud T Al-Jamal and David McCarthy and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1039/b712350g},
issn = {1359-7345},
year = {2008},
date = {2008-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {4},
pages = {459--461},
abstract = {Multiwalled carbon nanotube aqueous dispersions using block copolymers are able to form supramolecular complexes with the aromatic chromophore and anticancer agent doxorubicin via pi-pi stacking and enhance its cytotoxic activity.},
keywords = {Antineoplastic Agents, Breast Neoplasms, carbon, Cultured, Doxorubicin, Electron, Humans, I2CT, Microscopy, Nanotubes, Team-Bianco, Transmission, Tumor Cells},
pubstate = {published},
tppubtype = {article}
}
Nehme Nadine T, Liégeois Samuel, Kele Beatrix, Giammarinaro Philippe, Pradel Elizabeth, Hoffmann Jules A, Ewbank Jonathan J, Ferrandon Dominique
A model of bacterial intestinal infections in Drosophila melanogaster Journal Article
In: PLoS Pathog., vol. 3, no. 11, pp. e173, 2007, ISSN: 1553-7374.
Abstract | Links | BibTeX | Tags: Animal, Animals, Disease Models, Electron, ferrandon, fluorescence, Hemolymph, hoffmann, Host-Pathogen Interactions, Immunohistochemistry, Intestines, M3i, Microscopy, Reverse Transcriptase Polymerase Chain Reaction, Serratia Infections, Serratia marcescens, Transmission
@article{nehme_model_2007b,
title = {A model of bacterial intestinal infections in Drosophila melanogaster},
author = {Nadine T Nehme and Samuel Liégeois and Beatrix Kele and Philippe Giammarinaro and Elizabeth Pradel and Jules A Hoffmann and Jonathan J Ewbank and Dominique Ferrandon},
doi = {10.1371/journal.ppat.0030173},
issn = {1553-7374},
year = {2007},
date = {2007-01-01},
journal = {PLoS Pathog.},
volume = {3},
number = {11},
pages = {e173},
abstract = {Serratia marcescens is an entomopathogenic bacterium that opportunistically infects a wide range of hosts, including humans. In a model of septic injury, if directly introduced into the body cavity of Drosophila, this pathogen is insensitive to the host's systemic immune response and kills flies in a day. We find that S. marcescens resistance to the Drosophila immune deficiency (imd)-mediated humoral response requires the bacterial lipopolysaccharide O-antigen. If ingested by Drosophila, bacteria cross the gut and penetrate the body cavity. During this passage, the bacteria can be observed within the cells of the intestinal epithelium. In such an oral infection model, the flies succumb to infection only after 6 days. We demonstrate that two complementary host defense mechanisms act together against such food-borne infection: an antimicrobial response in the intestine that is regulated by the imd pathway and phagocytosis by hemocytes of bacteria that have escaped into the hemolymph. Interestingly, bacteria present in the hemolymph elicit a systemic immune response only when phagocytosis is blocked. Our observations support a model wherein peptidoglycan fragments released during bacterial growth activate the imd pathway and do not back a proposed role for phagocytosis in the immune activation of the fat body. Thanks to the genetic tools available in both host and pathogen, the molecular dissection of the interactions between S. marcescens and Drosophila will provide a useful paradigm for deciphering intestinal pathogenesis.},
keywords = {Animal, Animals, Disease Models, Electron, ferrandon, fluorescence, Hemolymph, hoffmann, Host-Pathogen Interactions, Immunohistochemistry, Intestines, M3i, Microscopy, Reverse Transcriptase Polymerase Chain Reaction, Serratia Infections, Serratia marcescens, Transmission},
pubstate = {published},
tppubtype = {article}
}
Shiao Shin-Hong, Whitten Miranda M A, Zachary Daniel, Hoffmann Jules A, Levashina Elena A
Fz2 and cdc42 mediate melanization and actin polymerization but are dispensable for Plasmodium killing in the mosquito midgut Journal Article
In: PLoS Pathog., vol. 2, no. 12, pp. e133, 2006, ISSN: 1553-7374.
Abstract | Links | BibTeX | Tags: Actins, Animals, Anopheles, Carrier Proteins, cdc42 GTP-Binding Protein, Double-Stranded, Electron, Frizzled Receptors, Gastrointestinal Tract, hoffmann, Host-Parasite Interactions, Immunity, Innate, Insect Vectors, Intestinal Mucosa, M3i, Melanins, Microarray Analysis, Microscopy, Plasmodium berghei, Polymers, Protozoan, RNA, scanning, telomerase
@article{shiao_fz2_2006,
title = {Fz2 and cdc42 mediate melanization and actin polymerization but are dispensable for Plasmodium killing in the mosquito midgut},
author = {Shin-Hong Shiao and Miranda M A Whitten and Daniel Zachary and Jules A Hoffmann and Elena A Levashina},
doi = {10.1371/journal.ppat.0020133},
issn = {1553-7374},
year = {2006},
date = {2006-12-01},
journal = {PLoS Pathog.},
volume = {2},
number = {12},
pages = {e133},
abstract = {The midgut epithelium of the mosquito malaria vector Anopheles is a hostile environment for Plasmodium, with most parasites succumbing to host defenses. This study addresses morphological and ultrastructural features associated with Plasmodium berghei ookinete invasion in Anopheles gambiae midguts to define the sites and possible mechanisms of parasite killing. We show by transmission electron microscopy and immunofluorescence that the majority of ookinetes are killed in the extracellular space. Dead or dying ookinetes are surrounded by a polymerized actin zone formed within the basal cytoplasm of adjacent host epithelial cells. In refractory strain mosquitoes, we found that formation of this zone is strongly linked to prophenoloxidase activation leading to melanization. Furthermore, we identify two factors controlling both phenomena: the transmembrane receptor frizzled-2 and the guanosine triphosphate-binding protein cell division cycle 42. However, the disruption of actin polymerization and melanization by double-stranded RNA inhibition did not affect ookinete survival. Our results separate the mechanisms of parasite killing from subsequent reactions manifested by actin polymerization and prophenoloxidase activation in the A. gambiae-P. berghei model. These latter processes are reminiscent of wound healing in other organisms, and we propose that they represent a form of wound-healing response directed towards a moribund ookinete, which is perceived as damaged tissue.},
keywords = {Actins, Animals, Anopheles, Carrier Proteins, cdc42 GTP-Binding Protein, Double-Stranded, Electron, Frizzled Receptors, Gastrointestinal Tract, hoffmann, Host-Parasite Interactions, Immunity, Innate, Insect Vectors, Intestinal Mucosa, M3i, Melanins, Microarray Analysis, Microscopy, Plasmodium berghei, Polymers, Protozoan, RNA, scanning, telomerase},
pubstate = {published},
tppubtype = {article}
}
Singh Ravi, Pantarotto Davide, Lacerda Lara, Pastorin Giorgia, Klumpp Cédric, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers Journal Article
In: Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 9, pp. 3357–3362, 2006, ISSN: 0027-8424.
Abstract | Links | BibTeX | Tags: Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission
@article{singh_tissue_2006,
title = {Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers},
author = {Ravi Singh and Davide Pantarotto and Lara Lacerda and Giorgia Pastorin and Cédric Klumpp and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1073/pnas.0509009103},
issn = {0027-8424},
year = {2006},
date = {2006-02-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {103},
number = {9},
pages = {3357--3362},
abstract = {Carbon nanotubes (CNT) are intensively being developed for biomedical applications including drug and gene delivery. Although all possible clinical applications will require compatibility of CNT with the biological milieu, their in vivo capabilities and limitations have not yet been explored. In this work, water-soluble, single-walled CNT (SWNT) have been functionalized with the chelating molecule diethylentriaminepentaacetic (DTPA) and labeled with indium ((111)In) for imaging purposes. Intravenous (i.v.) administration of these functionalized SWNT (f-SWNT) followed by radioactivity tracing using gamma scintigraphy indicated that f-SWNT are not retained in any of the reticuloendothelial system organs (liver or spleen) and are rapidly cleared from systemic blood circulation through the renal excretion route. The observed rapid blood clearance and half-life (3 h) of f-SWNT has major implications for all potential clinical uses of CNT. Moreover, urine excretion studies using both f-SWNT and functionalized multiwalled CNT followed by electron microscopy analysis of urine samples revealed that both types of nanotubes were excreted as intact nanotubes. This work describes the pharmacokinetic parameters of i.v. administered functionalized CNT relevant for various therapeutic and diagnostic applications.},
keywords = {Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Applications of carbon nanotubes in drug delivery Journal Article
In: Current Opinion in Chemical Biology, vol. 9, no. 6, pp. 674–679, 2005, ISSN: 1367-5931.
Abstract | Links | BibTeX | Tags: Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco
@article{bianco_applications_2005,
title = {Applications of carbon nanotubes in drug delivery},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1016/j.cbpa.2005.10.005},
issn = {1367-5931},
year = {2005},
date = {2005-12-01},
journal = {Current Opinion in Chemical Biology},
volume = {9},
number = {6},
pages = {674--679},
abstract = {The development of new and efficient drug delivery systems is of fundamental importance to improve the pharmacological profiles of many classes of therapeutic molecules. Many different types of drug delivery systems are currently available. Within the family of nanomaterials, carbon nanotubes (CNT) have emerged as a new alternative and efficient tool for transporting and translocating therapeutic molecules. CNT can be functionalised with bioactive peptides, proteins, nucleic acids and drugs, and used to deliver their cargos to cells and organs. Because functionalised CNT display low toxicity and are not immunogenic, such systems hold great potential in the field of nanobiotechnology and nanomedicine.},
keywords = {Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Singh Ravi, Pantarotto Davide, McCarthy David, Chaloin Olivier, Hoebeke Johan, Partidos Charalambos D, Briand Jean-Paul, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors Journal Article
In: Journal of the American Chemical Society, vol. 127, no. 12, pp. 4388–4396, 2005, ISSN: 0002-7863.
Abstract | Links | BibTeX | Tags: carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco
@article{singh_binding_2005,
title = {Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors},
author = {Ravi Singh and Davide Pantarotto and David McCarthy and Olivier Chaloin and Johan Hoebeke and Charalambos D Partidos and Jean-Paul Briand and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1021/ja0441561},
issn = {0002-7863},
year = {2005},
date = {2005-03-01},
journal = {Journal of the American Chemical Society},
volume = {127},
number = {12},
pages = {4388--4396},
abstract = {Carbon nanotubes (CNTs) constitute a class of nanomaterials that possess characteristics suitable for a variety of possible applications. Their compatibility with aqueous environments has been made possible by the chemical functionalization of their surface, allowing for exploration of their interactions with biological components including mammalian cells. Functionalized CNTs (f-CNTs) are being intensively explored in advanced biotechnological applications ranging from molecular biosensors to cellular growth substrates. We have been exploring the potential of f-CNTs as delivery vehicles of biologically active molecules in view of possible biomedical applications, including vaccination and gene delivery. Recently we reported the capability of ammonium-functionalized single-walled CNTs to penetrate human and murine cells and facilitate the delivery of plasmid DNA leading to expression of marker genes. To optimize f-CNTs as gene delivery vehicles, it is essential to characterize their interactions with DNA. In the present report, we study the interactions of three types of f-CNTs, ammonium-functionalized single-walled and multiwalled carbon nanotubes (SWNT-NH3+; MWNT-NH3+), and lysine-functionalized single-walled carbon nanotubes (SWNT-Lys-NH3+), with plasmid DNA. Nanotube-DNA complexes were analyzed by scanning electron microscopy, surface plasmon resonance, PicoGreen dye exclusion, and agarose gel shift assay. The results indicate that all three types of cationic carbon nanotubes are able to condense DNA to varying degrees, indicating that both nanotube surface area and charge density are critical parameters that determine the interaction and electrostatic complex formation between f-CNTs with DNA. All three different f-CNT types in this study exhibited upregulation of marker gene expression over naked DNA using a mammalian (human) cell line. Differences in the levels of gene expression were correlated with the structural and biophysical data obtained for the f-CNT:DNA complexes to suggest that large surface area leading to very efficient DNA condensation is not necessary for effective gene transfer. However, it will require further investigation to determine whether the degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo. This study constitutes the first thorough investigation into the physicochemical interactions between cationic functionalized carbon nanotubes and DNA toward construction of carbon nanotube-based gene transfer vector systems.},
keywords = {carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Hetru Charles, Letellier L, Oren Z, Hoffmann Jules A, Shai Y
Androctonin, a hydrophilic disulphide-bridged non-haemolytic anti-microbial peptide: a plausible mode of action Journal Article
In: Biochem. J., vol. 345 Pt 3, pp. 653–664, 2000, ISSN: 0264-6021.
Abstract | BibTeX | Tags: Adenosine Triphosphate, Anti-Bacterial Agents, Cations, Cell Membrane Permeability, Cytoplasm, Disulfides, Electron, Escherichia coli, Fluoresceins, Fluorescent Dyes, Fourier Transform Infrared, Gram-Negative Bacteria, hoffmann, Insect Proteins, Liposomes, M3i, Microbial Sensitivity Tests, Micrococcus luteus, Microscopy, oxygen, Phospholipids, Potassium, Proteins, spectroscopy
@article{hetru_androctonin_2000,
title = {Androctonin, a hydrophilic disulphide-bridged non-haemolytic anti-microbial peptide: a plausible mode of action},
author = {Charles Hetru and L Letellier and Z Oren and Jules A Hoffmann and Y Shai},
issn = {0264-6021},
year = {2000},
date = {2000-01-01},
journal = {Biochem. J.},
volume = {345 Pt 3},
pages = {653--664},
abstract = {Androctonin is a 25-residue non-haemolytic anti-microbial peptide isolated from the scorpion Androctonus australis and contains two disulphide bridges. Androctonin is different from known native anti-microbial peptides, being a relatively hydrophilic and non-amphipathic molecule. This raises the possibility that the target of androctonin might not be the bacterial membrane, shown to be a target for most amphipathic lytic peptides. To shed light on its mode of action on bacteria and its non-haemolytic activity, we synthesized androctonin, its fluorescent derivatives and its all-D-amino acid enantiomer. The enantiomer preserved high activity, suggesting a lipid-peptide interaction between androctonin and bacterial membranes. In Gram-positive and (at higher concentrations) Gram-negative bacteria, androctonin induced an immediate perturbation of the permeability properties of the cytoplasmic membrane of the bacterial energetic state, concomitant with perturbation of the morphology of the cell envelope as revealed by electron microscopy. Androctonin binds only to negatively charged lipid vesicles and induces the leakage of markers at high concentrations and with a slow kinetics, in contrast with amphipathic alpha-helical anti-microbial peptides that bind and permeate negatively charged vesicles, and to a smaller extent also zwitterionic ones. This might explain the selective lytic activity of androctonin towards bacteria but not red blood cells. Polarized attenuated total reflection-Fourier transform infrared spectroscopy revealed that androctonin adopts a beta-sheet structure in membranes and did not affect the lipid acyl chain order, which supports a detergent-like effect. The small size of androctonin, its hydrophilic character and its physicochemical properties are favourable features for its potential application as a replacement for commercially available antibiotics to which bacteria have developed resistance.},
keywords = {Adenosine Triphosphate, Anti-Bacterial Agents, Cations, Cell Membrane Permeability, Cytoplasm, Disulfides, Electron, Escherichia coli, Fluoresceins, Fluorescent Dyes, Fourier Transform Infrared, Gram-Negative Bacteria, hoffmann, Insect Proteins, Liposomes, M3i, Microbial Sensitivity Tests, Micrococcus luteus, Microscopy, oxygen, Phospholipids, Potassium, Proteins, spectroscopy},
pubstate = {published},
tppubtype = {article}
}
Ehret-Sabatier L, Loew D, Goyffon M, Fehlbaum P, Hoffmann Jules A, van Dorsselaer A, Bulet Philippe
Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood Journal Article
In: J. Biol. Chem., vol. 271, no. 47, pp. 29537–29544, 1996, ISSN: 0021-9258.
Abstract | BibTeX | Tags: Animals, Anti-Bacterial Agents, Chromatography, Cysteine, Electron, Hemolymph, Hemolysis, High Pressure Liquid, hoffmann, M3i, Mass Spectrometry, Microscopy, Peptides, Scorpions
@article{ehret-sabatier_characterization_1996,
title = {Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood},
author = {L Ehret-Sabatier and D Loew and M Goyffon and P Fehlbaum and Jules A Hoffmann and A van Dorsselaer and Philippe Bulet},
issn = {0021-9258},
year = {1996},
date = {1996-11-01},
journal = {J. Biol. Chem.},
volume = {271},
number = {47},
pages = {29537--29544},
abstract = {We have isolated, from the hemolymph of unchallenged scorpions of the species Androctonus australis, three distinct antimicrobial peptides, which we have fully characterized by Edman degradation, electrospray ionization mass spectrometry, and matrix-assisted laser desorption/ionization mass spectrometry. Two are novel molecules: (i) androctonin, a 25-residue peptide with two disulfide bridges, active against both bacteria (Gram-positive and Gram-negative) and fungi and showing marked sequence homology to tachyplesins and polyphemusins from horseshoe crabs; and (ii) buthinin, a 34-residue antibacterial (Gram-positive and Gram-negative) peptide with three disulfide bridges. The third peptide contains 37 residues and three disulfide bridges and clearly belongs to the family of anti-Gram-positive insect defensins. We have synthesized androctonin and explored its activity spectrum and mode of action.},
keywords = {Animals, Anti-Bacterial Agents, Chromatography, Cysteine, Electron, Hemolymph, Hemolysis, High Pressure Liquid, hoffmann, M3i, Mass Spectrometry, Microscopy, Peptides, Scorpions},
pubstate = {published},
tppubtype = {article}
}
Brehélin M, Zachary Daniel, Hoffmann Jules A
A comparative ultrastructural study of blood cells from nine insect orders Journal Article
In: Cell Tissue Res., vol. 195, no. 1, pp. 45–57, 1978, ISSN: 0302-766X.
Abstract | BibTeX | Tags: Animals, Blood Cells, Electron, Granulocytes, Hemocytes, hoffmann, insects, M3i, Microscopy, Species Specificity
@article{brehelin_comparative_1978,
title = {A comparative ultrastructural study of blood cells from nine insect orders},
author = {M Brehélin and Daniel Zachary and Jules A Hoffmann},
issn = {0302-766X},
year = {1978},
date = {1978-01-01},
journal = {Cell Tissue Res.},
volume = {195},
number = {1},
pages = {45--57},
abstract = {An ultrastructural study of hemocytes from 9 different insect orders has led to the identification of 8 cell types: (1) Plasmatocytes, whose cytoplasm is filled with small dense lysosomes and large heterogeneous structures, are phagocytic cells. (2) Granulocytes, filled with uniformly electron dense granules, are involved in capsule formation. (3) Coagulocytes, which contain granules and structured globules and which possess a well developed RER, are involved in phagocytosis. (4) Spherule cells are filled with large spherical inclusions. (5) Oenocytoids are large cells with few cytoplasmic organelles. These 5 hemocyte types represent the majority of insect blood cells. (6) Prohemocytes, blastic cells which are one of the stem cells a hemocytes, are very few in number in each species investigated. (7) Thrombocytoids and (8) Prodocytes are restricted to a small number of insect species. The ultrastructural characteristics of these hemocyte types are discussed.},
keywords = {Animals, Blood Cells, Electron, Granulocytes, Hemocytes, hoffmann, insects, M3i, Microscopy, Species Specificity},
pubstate = {published},
tppubtype = {article}
}
Zachary Daniel, Hoffmann Jules A
The haemocytes of Calliphora erythrocephala (Meig.) (Diptera) Journal Article
In: Z Zellforsch Mikrosk Anat, vol. 141, no. 1, pp. 55–73, 1973, ISSN: 0340-0336.
BibTeX | Tags: Animals, Cell Count, Cell Nucleus, Diptera, Electron, Endoplasmic Reticulum, Hemolymph, Hemostasis, hoffmann, Inclusion Bodies, Larva, Lipids, Lysosomes, M3i, Microscopy, mitochondria, Phase-Contrast, Pupa, Radiation Effects
@article{zachary_haemocytes_1973,
title = {The haemocytes of Calliphora erythrocephala (Meig.) (Diptera)},
author = {Daniel Zachary and Jules A Hoffmann},
issn = {0340-0336},
year = {1973},
date = {1973-07-01},
journal = {Z Zellforsch Mikrosk Anat},
volume = {141},
number = {1},
pages = {55--73},
keywords = {Animals, Cell Count, Cell Nucleus, Diptera, Electron, Endoplasmic Reticulum, Hemolymph, Hemostasis, hoffmann, Inclusion Bodies, Larva, Lipids, Lysosomes, M3i, Microscopy, mitochondria, Phase-Contrast, Pupa, Radiation Effects},
pubstate = {published},
tppubtype = {article}
}
Joly L, Weins M J, Hoffmann Jules A, Porte A
Development of the prothoracic glands of permanent larvae of Locusta migratoria obtained by selective irradiation of the hemocytopoietic tissue Journal Article
In: Z Zellforsch Mikrosk Anat, vol. 137, no. 3, pp. 387–397, 1973, ISSN: 0340-0336.
BibTeX | Tags: Age Factors, Animals, Biological, Ecdysone, Electron, Endocrine Glands, Golgi Apparatus, Grasshoppers, hoffmann, Larva, M3i, Metamorphosis, Microscopy, Radiation Effects, ribosomes
@article{joly_development_1973,
title = {Development of the prothoracic glands of permanent larvae of Locusta migratoria obtained by selective irradiation of the hemocytopoietic tissue},
author = {L Joly and M J Weins and Jules A Hoffmann and A Porte},
issn = {0340-0336},
year = {1973},
date = {1973-02-01},
journal = {Z Zellforsch Mikrosk Anat},
volume = {137},
number = {3},
pages = {387--397},
keywords = {Age Factors, Animals, Biological, Ecdysone, Electron, Endocrine Glands, Golgi Apparatus, Grasshoppers, hoffmann, Larva, M3i, Metamorphosis, Microscopy, Radiation Effects, ribosomes},
pubstate = {published},
tppubtype = {article}
}
Zachary Daniel, Hoffmann Jules A, Porte A
A new type of blood cell (the thrombocytoid) in Calliphora erythrocephala Journal Article
In: C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat., vol. 275, no. 3, pp. 393–395, 1972.
BibTeX | Tags: Animals, Blood Cells, Blood Platelets, Cell Membrane, Cell Nucleus, Cytoplasm, Diptera, Electron, Hemolymph, hoffmann, M3i, Microscopy, Phase-Contrast
@article{zachary_new_1972,
title = {A new type of blood cell (the thrombocytoid) in Calliphora erythrocephala},
author = {Daniel Zachary and Jules A Hoffmann and A Porte},
year = {1972},
date = {1972-07-01},
journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},
volume = {275},
number = {3},
pages = {393--395},
keywords = {Animals, Blood Cells, Blood Platelets, Cell Membrane, Cell Nucleus, Cytoplasm, Diptera, Electron, Hemolymph, hoffmann, M3i, Microscopy, Phase-Contrast},
pubstate = {published},
tppubtype = {article}
}
Hoffmann Jules A
The hemopoietic organs of the two orthopterans Locusta migratoria and Gryllus bimaculatus Journal Article
In: Z Zellforsch Mikrosk Anat, vol. 106, no. 3, pp. 451–472, 1970, ISSN: 0340-0336.
BibTeX | Tags: Animals, Cell Differentiation, Diptera, Electron, Hematopoiesis, Hematopoietic System, hoffmann, M3i, Microscopy, Species Specificity
@article{hoffmann_hemopoietic_1970,
title = {The hemopoietic organs of the two orthopterans Locusta migratoria and Gryllus bimaculatus},
author = {Jules A Hoffmann},
issn = {0340-0336},
year = {1970},
date = {1970-01-01},
journal = {Z Zellforsch Mikrosk Anat},
volume = {106},
number = {3},
pages = {451--472},
keywords = {Animals, Cell Differentiation, Diptera, Electron, Hematopoiesis, Hematopoietic System, hoffmann, M3i, Microscopy, Species Specificity},
pubstate = {published},
tppubtype = {article}
}