RNA interference and receptors

@article{,

title = {Reverse transcription of the yeast Ty1 retrotransposon: the mode of first strand transfer is either intermolecular or intramolecular},

author = {M Wilhelm and M Boutabout and T Heyman and F X Wilhelm},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10329158},

isbn = {10329158},

year  = {1999},

date = {1999-01-01},

journal = {J Mol Biol},

volume = {288},

number = {4},

pages = {505-510},

abstract = {Replication of the yeast Ty1 retrotransposon occurs by a mechanism similar to that of retroviruses. According to the current model of retroviral reverse transcription, two strand transfers (the so-called minus-strand and plus-strand strong-stop DNA transfers) are required to produce full-length preintegrative DNA. Because two genomic RNA molecules are packaged inside the viral particles, the strand transfers can be either intra- or intermolecular. To study the mode of transfer of minus-strand strong-stop DNA during reverse transcription of the yeast Ty1 retrotransposon, we have analyzed the cDNA products that accumulate in the cytoplasmic virus-like particles of yeast cells harboring two marked Ty1 elements. Our results indicate that Ty1 minus-strand transfer occurs in a random manner with approximately similar frequencies of intra- and intermolecular transfer. It has been observed recently that intra- and intermolecular minus-strand transfer occur at similar frequencies during replication of a complex retrovirus such as HIV-1. These results together with the observation that genetic recombination occurs with a high frequency during minus-strand synthesis suggest that both packaged RNA molecules are needed for the synthesis of one minus-strand DNA.},

note = {0022-2836 

Journal Article},

keywords = {Base Sequence  DNA, Genetic, Non-U.S. Gov't  *Transcription, Nucleic Acid  *Retroelements  Saccharomyces cerevisiae/*genetics  Support, Single-Stranded/genetics  Repetitive Sequences, Unité ARN},

pubstate = {published},

tppubtype = {article}

}