Publications
2007
Winter F., Edaye S., Huttenhofer A., Brunel C.
Anopheles gambiae miRNAs as actors of defence reaction against Plasmodium invasion Journal Article
In: Nucleic Acids Res, vol. 35, no. 20, pp. 6953-62, 2007, (1362-4962 (Electronic) 0305-1048 (Linking) Journal Article Research Support, Non-U.S. Gov't).
Abstract | BibTeX | Tags: *Gene, Animals, Anopheles, BRUNEL, Digestive, Expression, Female, gambiae/*genetics/*immunology/parasitology, Gene, III/genetics, Library, Male, MicroRNAs/*immunology, Plasmodium/*immunology, Profiling, Ribonuclease, silencing, System/immunology/metabolism/parasitology
@article{,
title = {Anopheles gambiae miRNAs as actors of defence reaction against Plasmodium invasion},
author = { F. Winter and S. Edaye and A. Huttenhofer and C. Brunel},
year = {2007},
date = {2007-01-01},
journal = {Nucleic Acids Res},
volume = {35},
number = {20},
pages = {6953-62},
abstract = {The path Plasmodium takes across the Anopheles midgut constitutes the major bottleneck during the malaria transmission cycle. In the present study, using a combination of shot-gun cloning and bioinformatic analysis, we have identified 18 miRNAs from Anopheles gambiae including three miRNAs unique to mosquito. Twelve of them are expressed ubiquitously across the body, independently of gender, while the other six exhibited an expression pattern restricted to the digestive system. Strikingly, the expression patterns of four miRNAs, including the three unique to mosquito, are affected by the presence of Plasmodium. We also show that knocking down Dicer1 and Ago1 mRNAs led to an increased sensitivity to Plasmodium infection. Altogether, these data support an involvement of miRNAs as new layers in the regulation of Anopheles defence reaction.},
note = {1362-4962 (Electronic)
0305-1048 (Linking)
Journal Article
Research Support, Non-U.S. Gov't},
keywords = {*Gene, Animals, Anopheles, BRUNEL, Digestive, Expression, Female, gambiae/*genetics/*immunology/parasitology, Gene, III/genetics, Library, Male, MicroRNAs/*immunology, Plasmodium/*immunology, Profiling, Ribonuclease, silencing, System/immunology/metabolism/parasitology},
pubstate = {published},
tppubtype = {article}
}
2004
Popiela A., Keith G., Borzecki A., Popiela G., Manowiec M., Gabrys M.
The meaning of the methylation of genomic DNA in the regulation of gene expression levels Journal Article
In: Eur J Gynaecol Oncol, vol. 25, no. 2, pp. 145-9, 2004, (0392-2936 Journal Article Review Review, Tutorial).
Abstract | BibTeX | Tags: *DNA, *Gene, Expression, Human, KEITH, Methylation, Neoplasms/*genetics, Neoplastic, Regulation
@article{,
title = {The meaning of the methylation of genomic DNA in the regulation of gene expression levels},
author = { A. Popiela and G. Keith and A. Borzecki and G. Popiela and M. Manowiec and M. Gabrys},
year = {2004},
date = {2004-01-01},
journal = {Eur J Gynaecol Oncol},
volume = {25},
number = {2},
pages = {145-9},
abstract = {INTRODUCTION: Methylation of genomic DNA is one of the major mechanisms that deactivates genes and regulates their tissue-specific transcription levels. Its patterns are based on clonal inheritance that occurs in the early stages of embryogenesis. All changes in the DNA methylation levels occurring especially in the promoter region of the genes, which involve hypo- as well as hyper-methylation, lead to cell differentiation and growth disorders. Therefore it can become an impulse that initiates different pathological processes including carcinogenesis. MATERIAL AND METHODS: The purpose of this review was to present the recent knowledge concerning methylation of genomic DNA based on recent references and authors' experience. RESULTS AND CONCLUSION: Genome stability disorders could be caused either by mutations, which damage the structure of the genes and have not been formerly removed, or as the consequence of an epigenetic mechanism. Methylation plays a decisive role in the activity of many genes and could be a natural weapon of an organism against the expression of foreign genetic material that degrades the original genome structure.},
note = {0392-2936
Journal Article
Review
Review, Tutorial},
keywords = {*DNA, *Gene, Expression, Human, KEITH, Methylation, Neoplasms/*genetics, Neoplastic, Regulation},
pubstate = {published},
tppubtype = {article}
}
2002
Cristofari G., Bampi C., Wilhelm M., Wilhelm F. X., Darlix J. L.
A 5'-3' long-range interaction in Ty1 RNA controls its reverse transcription and retrotransposition Journal Article
In: EMBO J, vol. 21, no. 16, pp. 4368-79, 2002, (0261-4189 Journal Article).
Abstract | BibTeX | Tags: *Gene, *Transcription, Acid, cerevisiae/*genetics, Complementary/biosynthesis, Conformation, DNA, Expression, Fungal, Fungal/chemistry/*metabolism, Genetic, Gov't, in, Messenger/chemistry/*metabolism, Non-U.S., Nucleic, Phylogeny, Regulation, Retroelements/*genetics, RNA, Saccharomyces, Support, vitro
@article{,
title = {A 5'-3' long-range interaction in Ty1 RNA controls its reverse transcription and retrotransposition},
author = { G. Cristofari and C. Bampi and M. Wilhelm and F. X. Wilhelm and J. L. Darlix},
year = {2002},
date = {2002-01-01},
journal = {EMBO J},
volume = {21},
number = {16},
pages = {4368-79},
abstract = {LTR-retrotransposons are abundant components of all eukaryotic genomes and appear to be key players in their evolution. They share with retroviruses a reverse transcription step during their replication cycle. To better understand the replication of retrotransposons as well as their similarities to and differences from retroviruses, we set up an in vitro model system to examine minus-strand cDNA synthesis of the yeast Ty1 LTR-retrotransposon. Results show that the 5' and 3' ends of Ty1 genomic RNA interact through 14 nucleotide 5'-3' complementary sequences (CYC sequences). This 5'-3' base pairing results in an efficient initiation of reverse transcription in vitro. Transposition of a marked Ty1 element and Ty1 cDNA synthesis in yeast rely on the ability of the CYC sequences to base pair. This 5'-3' interaction is also supported by phylogenic analysis of all full-length Ty1 and Ty2 elements present in the Saccharomyces cerevisiae genome. These novel findings lead us to propose that circularization of the Ty1 genomic RNA controls initiation of reverse transcription and may limit reverse transcription of defective retroelements.},
note = {0261-4189
Journal Article},
keywords = {*Gene, *Transcription, Acid, cerevisiae/*genetics, Complementary/biosynthesis, Conformation, DNA, Expression, Fungal, Fungal/chemistry/*metabolism, Genetic, Gov't, in, Messenger/chemistry/*metabolism, Non-U.S., Nucleic, Phylogeny, Regulation, Retroelements/*genetics, RNA, Saccharomyces, Support, vitro},
pubstate = {published},
tppubtype = {article}
}
Mohr S., Leikauf G. D., Keith G., Rihn B. H.
Microarrays as cancer keys: an array of possibilities Journal Article
In: J Clin Oncol, vol. 20, no. 14, pp. 3165-75, 2002, (0732-183x Journal Article Review Review, Tutorial).
Abstract | BibTeX | Tags: (Genetics), *Gene, *Oligonucleotide, *Sequence, Aberrations, Analysis, Analysis/methods, Animals, Array, Chromosome, DNA/methods, Expression, Genotype, Gov't, Human, Mutation, Neoplasms/*genetics, Neoplastic, Oncogenes/*genetics, P.H.S., Polymorphism, Profiling/methods, Proteome/genetics, Regulation, Sequence, Support, U.S.
@article{,
title = {Microarrays as cancer keys: an array of possibilities},
author = { S. Mohr and G. D. Leikauf and G. Keith and B. H. Rihn},
year = {2002},
date = {2002-01-01},
journal = {J Clin Oncol},
volume = {20},
number = {14},
pages = {3165-75},
abstract = {Malignant transformation results from accumulation of genetic and epigenetic events. Functional studies of cancer will be crucial to our understanding of its complexity and polymorphism. There is no doubt that emerging genomic and proteomic technologies will facilitate such investigations. Microarray technology is a new and efficient approach to extract data of biomedical relevance for a wide range of applications. In cancer research, it will provide high-throughput and valuable insights into differences in an individual's tumor as compared with constitutional DNA, mRNA expression, and protein expression and activity. Across individuals, comparisons could provide tissue-specific disease signatures that provide diagnosis based on hundreds of informative genes. The resulting product should be a wealth of tumor-associated and tumor-specific biomarkers, which may help in cancer etiology, diagnosis, and therapy and ultimately lead to "molecular nosology" of cancers. This review highlights the recent developments in microarray technologies in cancer research, focuses on the results obtained so far, and describes the eventual use of microarray technology for clinical applications.},
note = {0732-183x
Journal Article
Review
Review, Tutorial},
keywords = {(Genetics), *Gene, *Oligonucleotide, *Sequence, Aberrations, Analysis, Analysis/methods, Animals, Array, Chromosome, DNA/methods, Expression, Genotype, Gov't, Human, Mutation, Neoplasms/*genetics, Neoplastic, Oncogenes/*genetics, P.H.S., Polymorphism, Profiling/methods, Proteome/genetics, Regulation, Sequence, Support, U.S.},
pubstate = {published},
tppubtype = {article}
}
2000
Rihn B. H., Mohr S., McDowell S. A., Binet S., Loubinoux J., Galateau F., Keith G., Leikauf G. D.
Differential gene expression in mesothelioma Journal Article
In: FEBS Lett, vol. 480, no. 2-3, pp. 95-100, 2000, (0014-5793 Journal Article).
Abstract | BibTeX | Tags: *Gene, Adhesion, Analysis/methods, Array, Cell, Cells, Chain, Cultured, Cycle, Division, Expression, Gene, Human, Invasiveness, Mesothelioma/*genetics/metabolism, Neoplasm, Neoplastic, Oligonucleotide, Oxidative, Polymerase, Profiling, Proteins/metabolism, Reaction, Regulation, Reverse, Sequence, Stress, Transcriptase, tumor, Xenobiotics
@article{,
title = {Differential gene expression in mesothelioma},
author = { B. H. Rihn and S. Mohr and S. A. McDowell and S. Binet and J. Loubinoux and F. Galateau and G. Keith and G. D. Leikauf},
year = {2000},
date = {2000-01-01},
journal = {FEBS Lett},
volume = {480},
number = {2-3},
pages = {95-100},
abstract = {To investigate the molecular events controlling malignant transformation of human pleural cells, we compared constitutive gene expression of mesothelioma cells to that of pleural cells. Using cDNA microarray and high-density filter array, we assessed expression levels of > 6500 genes. Most of the highly expressed transcripts were common to both cell lines and included genes associated with stress response and DNA repair, outcomes consistent with the radio- and chemo-resistance of mesothelioma. Interestingly, of the fewer than 300 genes that differed between cell lines, most functioned in (i) macromolecule stability, (ii) cell adhesion and recognition, (iii) cell migration (invasiveness), and (iv) extended cell division. Expression levels of several of these genes were confirmed by RT-PCR and could be useful as diagnostic markers of human mesothelioma.},
note = {0014-5793
Journal Article},
keywords = {*Gene, Adhesion, Analysis/methods, Array, Cell, Cells, Chain, Cultured, Cycle, Division, Expression, Gene, Human, Invasiveness, Mesothelioma/*genetics/metabolism, Neoplasm, Neoplastic, Oligonucleotide, Oxidative, Polymerase, Profiling, Proteins/metabolism, Reaction, Regulation, Reverse, Sequence, Stress, Transcriptase, tumor, Xenobiotics},
pubstate = {published},
tppubtype = {article}
}