Publications
2004
de la Pena-Lefebvre P Garcia, Chanseaud Y, Tamby M C, Reinbolt J, Batteux F, Allanore Y, Kahan A, Meyer O, Benveniste O, Boyer O, Guillevin L, Boissier M C, Mouthon L
In: Clin Immunol, vol. 111, no. 3, pp. 241-251, 2004, ISBN: 15183145, (1521-6616 Journal Article).
Abstract | Links | BibTeX | Tags: EHRESMANN Autoantibodies/*analysis Blotting, Non-U.S. Gov't, Polyacrylamide Gel Endothelial Cells/*immunology Enzyme-Linked Immunosorbent Assay Female Human Immunoglobulin G/analysis Immunoglobulin M/analysis Male Middle Aged Scleroderma, Systemic/*immunology Support, Type I/*immunology Electrophoresis, Unité ARN, Western Centromere/immunology DNA Topoisomerases
@article{,
title = {IgG reactivity with a 100-kDa tissue and endothelial cell antigen identified as topoisomerase 1 distinguishes between limited and diffuse systemic sclerosis patients},
author = {P Garcia de la Pena-Lefebvre and Y Chanseaud and M C Tamby and J Reinbolt and F Batteux and Y Allanore and A Kahan and O Meyer and O Benveniste and O Boyer and L Guillevin and M C Boissier and L Mouthon},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15183145},
isbn = {15183145},
year = {2004},
date = {2004-01-01},
journal = {Clin Immunol},
volume = {111},
number = {3},
pages = {241-251},
abstract = {We have analyzed antibody (Ab) reactivities of patients with limited systemic sclerosis (SSc) and anti-centromere Ab, patients with diffuse SSc and anti-topoisomerase 1 (anti-topo 1) Ab, patients with diffuse SSc without anti-topo 1 or anti-centromere Ab and age- and gender-matched healthy controls with normal human tissue and endothelial cell (EC) antigens. IgG reactivities with tissue antigens differed significantly between patients with anti-topo 1 Ab and patients with anti-centromere Ab. One 100-kDa band identified as topoisomerase 1 in macrovascular and microvascular EC extracts was recognized by IgG from patients with anti-topo 1 Ab and 50% of patients without specific Ab. IgG from patients with limited SSc and anti-centromere Ab, but not those of other patients or controls specifically recognized a 80-kDa band only in microvascular EC. Our results indicate that Ab from patients with limited or diffuse SSc with or without anti-topo 1 Ab exhibit specific and mutually exclusive reactivity patterns.},
note = {1521-6616
Journal Article},
keywords = {EHRESMANN Autoantibodies/*analysis Blotting, Non-U.S. Gov't, Polyacrylamide Gel Endothelial Cells/*immunology Enzyme-Linked Immunosorbent Assay Female Human Immunoglobulin G/analysis Immunoglobulin M/analysis Male Middle Aged Scleroderma, Systemic/*immunology Support, Type I/*immunology Electrophoresis, Unité ARN, Western Centromere/immunology DNA Topoisomerases},
pubstate = {published},
tppubtype = {article}
}
We have analyzed antibody (Ab) reactivities of patients with limited systemic sclerosis (SSc) and anti-centromere Ab, patients with diffuse SSc and anti-topoisomerase 1 (anti-topo 1) Ab, patients with diffuse SSc without anti-topo 1 or anti-centromere Ab and age- and gender-matched healthy controls with normal human tissue and endothelial cell (EC) antigens. IgG reactivities with tissue antigens differed significantly between patients with anti-topo 1 Ab and patients with anti-centromere Ab. One 100-kDa band identified as topoisomerase 1 in macrovascular and microvascular EC extracts was recognized by IgG from patients with anti-topo 1 Ab and 50% of patients without specific Ab. IgG from patients with limited SSc and anti-centromere Ab, but not those of other patients or controls specifically recognized a 80-kDa band only in microvascular EC. Our results indicate that Ab from patients with limited or diffuse SSc with or without anti-topo 1 Ab exhibit specific and mutually exclusive reactivity patterns.