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2009
Herrero Antonia M, Toma Francesca M, Al-Jamal Khuloud T, Kostarelos Kostas, Bianco Alberto, Ros Tatiana Da, Bano Fouzia, Casalis Loredana, Scoles Giacinto, Prato Maurizio
Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery Journal Article
In: Journal of the American Chemical Society, vol. 131, no. 28, pp. 9843–9848, 2009, ISSN: 1520-5126.
Abstract | Links | BibTeX | Tags: Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water
@article{herrero_synthesis_2009,
title = {Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery},
author = {Antonia M Herrero and Francesca M Toma and Khuloud T Al-Jamal and Kostas Kostarelos and Alberto Bianco and Tatiana Da Ros and Fouzia Bano and Loredana Casalis and Giacinto Scoles and Maurizio Prato},
doi = {10.1021/ja903316z},
issn = {1520-5126},
year = {2009},
date = {2009-07-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {28},
pages = {9843--9848},
abstract = {A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.},
keywords = {Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water},
pubstate = {published},
tppubtype = {article}
}
A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.
Marega Riccardo, Aroulmoji Vincent, Dinon Francesca, Vaccari Lisa, Giordani Silvia, Bianco Alberto, Murano Erminio, Prato Maurizio
Diffusion-ordered NMR spectroscopy in the structural characterization of functionalized carbon nanotubes Journal Article
In: Journal of the American Chemical Society, vol. 131, no. 25, pp. 9086–9093, 2009, ISSN: 1520-5126.
Abstract | Links | BibTeX | Tags: carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Oxidation-Reduction, Surface Properties, Team-Bianco
@article{marega_diffusion-ordered_2009,
title = {Diffusion-ordered NMR spectroscopy in the structural characterization of functionalized carbon nanotubes},
author = {Riccardo Marega and Vincent Aroulmoji and Francesca Dinon and Lisa Vaccari and Silvia Giordani and Alberto Bianco and Erminio Murano and Maurizio Prato},
doi = {10.1021/ja902728w},
issn = {1520-5126},
year = {2009},
date = {2009-07-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {25},
pages = {9086--9093},
abstract = {The emerging applications of functionalized carbon nanotubes (CNTs) in various research domains necessitate the use of many different analytical techniques to confirm their structural modifications in a fast and reliable manner. Thus far, NMR spectroscopy has not been among the main tools for characterization of organically modified carbon nanostructures. (1)H analysis is limited because the signals in these derivatives are typically weak and broad, resulting in uncertainties of a few parts per million, and because of the strong interference of residual solvent signals. To overcome these limitations, we investigated the applicability of proton NMR spectroscopy based on gradient-edited diffusion pulse sequences (1D diffusion-ordered spectroscopy, DOSY) in the characterization of CNT derivatives. In general, diffusion NMR experiments allow the separation of NMR signals of different species present in a mixture, according to their own diffusion coefficients, merging spectroscopy information with size analysis. In the present study, a selected set of CNT derivatives was synthesized and analyzed using 1D DOSY experiments by applying strong magnetic field gradients (up to 42.6 G cm(-1)). Colorimetric tests (i.e., Kaiser test) and TGA analysis support the NMR findings, which are related to isolated and/or bundled short SWNTs, on the basis of TEM and AFM characterization. The overall results show that the diffusion-based NMR spectroscopy is a fast and promising approach for the characterization of covalently modified CNT derivatives.},
keywords = {carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Oxidation-Reduction, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The emerging applications of functionalized carbon nanotubes (CNTs) in various research domains necessitate the use of many different analytical techniques to confirm their structural modifications in a fast and reliable manner. Thus far, NMR spectroscopy has not been among the main tools for characterization of organically modified carbon nanostructures. (1)H analysis is limited because the signals in these derivatives are typically weak and broad, resulting in uncertainties of a few parts per million, and because of the strong interference of residual solvent signals. To overcome these limitations, we investigated the applicability of proton NMR spectroscopy based on gradient-edited diffusion pulse sequences (1D diffusion-ordered spectroscopy, DOSY) in the characterization of CNT derivatives. In general, diffusion NMR experiments allow the separation of NMR signals of different species present in a mixture, according to their own diffusion coefficients, merging spectroscopy information with size analysis. In the present study, a selected set of CNT derivatives was synthesized and analyzed using 1D DOSY experiments by applying strong magnetic field gradients (up to 42.6 G cm(-1)). Colorimetric tests (i.e., Kaiser test) and TGA analysis support the NMR findings, which are related to isolated and/or bundled short SWNTs, on the basis of TEM and AFM characterization. The overall results show that the diffusion-based NMR spectroscopy is a fast and promising approach for the characterization of covalently modified CNT derivatives.
Podesta Jennifer E, Al-Jamal Khuloud T, Herrero Antonia M, Tian Bowen, Ali-Boucetta Hanene, Hegde Vikas, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model Journal Article
In: Small (Weinheim an Der Bergstrasse, Germany), vol. 5, no. 10, pp. 1176–1185, 2009, ISSN: 1613-6829.
Abstract | Links | BibTeX | Tags: Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays
@article{podesta_antitumor_2009,
title = {Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model},
author = {Jennifer E Podesta and Khuloud T Al-Jamal and Antonia M Herrero and Bowen Tian and Hanene Ali-Boucetta and Vikas Hegde and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200801572},
issn = {1613-6829},
year = {2009},
date = {2009-05-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {5},
number = {10},
pages = {1176--1185},
abstract = {Carbon nanotubes are novel nanomaterials that are thought to offer potential benefits to a variety of biomedical and clinical applications. In this study, the treatment of a human lung carcinoma model in vivo using siRNA sequences leading to cytotoxicity and cell death is carried out using either cationic liposomes (DOTAP:cholesterol) or amino-functionalized multi-walled carbon nanotubes (MWNT - NH(+)(3)). Validation for the most cytotoxic siRNA sequence using a panel of human carcinoma and murine cells reveals that the proprietary siTOX sequence is human specific and can lead to significant cytotoxic activities delivered both by liposome or MWNT - NH(+)(3) in vitro. A comparative study using both types of vector indicates that only MWNT - NH(+)(3):siRNA complexes administered intratumorally can elicit delayed tumor growth and increased survival of xenograft-bearing animals. siTOX delivery via the cationic MWNT - NH(+)(3) is biologically active in vivo by triggering an apoptotic cascade, leading to extensive necrosis of the human tumor mass. This suggests that carbon-nanotube-mediated delivery of siRNA by intratumoral administration leads to successful and statistically significant suppression of tumor volume, followed by a concomitant prolongation of survival of human lung tumor-bearing animals. The direct comparison between carbon nanotubes and liposomes demonstrates the potential advantages offered by carbon nanotubes for the intracellular delivery of therapeutic agents in vivo. The present work may act as the impetus for further studies to explore the therapeutic capacity of chemically functionalized carbon nanotubes to deliver siRNA directly into the cytoplasm of target cells and achieve effective therapeutic silencing in various disease indications where local delivery is feasible or desirable.},
keywords = {Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are novel nanomaterials that are thought to offer potential benefits to a variety of biomedical and clinical applications. In this study, the treatment of a human lung carcinoma model in vivo using siRNA sequences leading to cytotoxicity and cell death is carried out using either cationic liposomes (DOTAP:cholesterol) or amino-functionalized multi-walled carbon nanotubes (MWNT - NH(+)(3)). Validation for the most cytotoxic siRNA sequence using a panel of human carcinoma and murine cells reveals that the proprietary siTOX sequence is human specific and can lead to significant cytotoxic activities delivered both by liposome or MWNT - NH(+)(3) in vitro. A comparative study using both types of vector indicates that only MWNT - NH(+)(3):siRNA complexes administered intratumorally can elicit delayed tumor growth and increased survival of xenograft-bearing animals. siTOX delivery via the cationic MWNT - NH(+)(3) is biologically active in vivo by triggering an apoptotic cascade, leading to extensive necrosis of the human tumor mass. This suggests that carbon-nanotube-mediated delivery of siRNA by intratumoral administration leads to successful and statistically significant suppression of tumor volume, followed by a concomitant prolongation of survival of human lung tumor-bearing animals. The direct comparison between carbon nanotubes and liposomes demonstrates the potential advantages offered by carbon nanotubes for the intracellular delivery of therapeutic agents in vivo. The present work may act as the impetus for further studies to explore the therapeutic capacity of chemically functionalized carbon nanotubes to deliver siRNA directly into the cytoplasm of target cells and achieve effective therapeutic silencing in various disease indications where local delivery is feasible or desirable.
Bianco Alberto
Potential usefulness of carbon nanotubes for cancer therapy Journal Article
In: Medecine Sciences: M/S, vol. 25, no. 2, pp. 125–127, 2009, ISSN: 0767-0974.
Links | BibTeX | Tags: carbon, Graphite, Humans, I2CT, Nanotubes, Neoplasms, Team-Bianco
@article{bianco_potential_2009,
title = {Potential usefulness of carbon nanotubes for cancer therapy},
author = {Alberto Bianco},
doi = {10.1051/medsci/2009252125},
issn = {0767-0974},
year = {2009},
date = {2009-02-01},
journal = {Medecine Sciences: M/S},
volume = {25},
number = {2},
pages = {125--127},
keywords = {carbon, Graphite, Humans, I2CT, Nanotubes, Neoplasms, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Gaillard Claire, Cellot Giada, Li Shouping, Toma Francesca Maria, Dumortier Hélène, Spalluto Giampiero, Cacciari Barbara, Prato Maurizio, Ballerini Laura, Bianco Alberto
Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality Journal Article
In: Advanced Materials, vol. 21, no. 28, pp. 2903–2908, 2009, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco
@article{gaillard_carbon_2009,
title = {Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality},
author = {Claire Gaillard and Giada Cellot and Shouping Li and Francesca Maria Toma and Hélène Dumortier and Giampiero Spalluto and Barbara Cacciari and Maurizio Prato and Laura Ballerini and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200900050},
doi = {10.1002/adma.200900050},
issn = {1521-4095},
year = {2009},
date = {2009-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {21},
number = {28},
pages = {2903--2908},
abstract = {Water-soluble carbon nanotubes functionalized with cell-adhesion peptides do not affect the viability of different cell types, including Jurkat cells, splenocytes, and neurons. They also do not modify the neuronal morphology and basic functions, thus representing a promising candidate for the exploitation of novel drug-delivery systems or for designing a new generation of self-assembling nerve bridges.},
keywords = {Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Water-soluble carbon nanotubes functionalized with cell-adhesion peptides do not affect the viability of different cell types, including Jurkat cells, splenocytes, and neurons. They also do not modify the neuronal morphology and basic functions, thus representing a promising candidate for the exploitation of novel drug-delivery systems or for designing a new generation of self-assembling nerve bridges.
Bianco Alberto
Les nanotubes de carbone : un nouvel outil contre le cancer Journal Article
In: M/S. Médecine sciences [ISSN papier : 0767-0974 ; ISSN numérique : 1958-5381], 2009, Vol. 25, N° 2; p. 125-127, 2009, ISSN: 1958-5381.
Links | BibTeX | Tags: I2CT, Team-Bianco
@article{bianco_les_2009,
title = {Les nanotubes de carbone : un nouvel outil contre le cancer},
author = {Alberto Bianco},
url = {http://www.ipubli.inserm.fr/handle/10608/6569},
doi = {10.1051/medsci/2009252125},
issn = {1958-5381},
year = {2009},
date = {2009-01-01},
urldate = {2020-04-01},
journal = {M/S. Médecine sciences [ISSN papier : 0767-0974 ; ISSN numérique : 1958-5381], 2009, Vol. 25, N° 2; p. 125-127},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Guichard Gilles, Trouche Nathalie, Wieckowski Sébastien, Sun Weimin, Chaloin Olivier, Bianco Alberto, Hoebeke Johan, Schneider Pascal, Fournel Sylvie
Rationally-designed multivalent architectures for mimicking homotrimers of CD40L, a member of the TNF superfamily Journal Article
In: Advances in Experimental Medicine and Biology, vol. 611, pp. 355–357, 2009, ISSN: 0065-2598.
Links | BibTeX | Tags: Biopolymers, CD40 Ligand, I2CT, Models, Molecular, Molecular Mimicry, Team-Bianco, X-Ray Diffraction
@article{guichard_rationally-designed_2009,
title = {Rationally-designed multivalent architectures for mimicking homotrimers of CD40L, a member of the TNF superfamily},
author = {Gilles Guichard and Nathalie Trouche and Sébastien Wieckowski and Weimin Sun and Olivier Chaloin and Alberto Bianco and Johan Hoebeke and Pascal Schneider and Sylvie Fournel},
doi = {10.1007/978-0-387-73657-0_157},
issn = {0065-2598},
year = {2009},
date = {2009-01-01},
journal = {Advances in Experimental Medicine and Biology},
volume = {611},
pages = {355--357},
keywords = {Biopolymers, CD40 Ligand, I2CT, Models, Molecular, Molecular Mimicry, Team-Bianco, X-Ray Diffraction},
pubstate = {published},
tppubtype = {article}
}
Geotti-Bianchini Piero, Crisma Marco, Peggion Cristina, Bianco Alberto, Formaggio Fernando
Conformationally controlled, thymine-based alpha-nucleopeptides Journal Article
In: Chemical Communications (Cambridge, England), no. 22, pp. 3178–3180, 2009, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: I2CT, Magnetic Resonance Spectroscopy, Oligopeptides, Protein Conformation, Team-Bianco, thymine, X-Ray Diffraction
@article{geotti-bianchini_conformationally_2009,
title = {Conformationally controlled, thymine-based alpha-nucleopeptides},
author = {Piero Geotti-Bianchini and Marco Crisma and Cristina Peggion and Alberto Bianco and Fernando Formaggio},
doi = {10.1039/b822789f},
issn = {1359-7345},
year = {2009},
date = {2009-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {22},
pages = {3178--3180},
abstract = {Rigid peptide backbones and backbone-to-side chain H-bonds permit the design of alpha-nucleopeptides with known 3D-structure; thymine-thymine base pairing is also observed.},
keywords = {I2CT, Magnetic Resonance Spectroscopy, Oligopeptides, Protein Conformation, Team-Bianco, thymine, X-Ray Diffraction},
pubstate = {published},
tppubtype = {article}
}
Rigid peptide backbones and backbone-to-side chain H-bonds permit the design of alpha-nucleopeptides with known 3D-structure; thymine-thymine base pairing is also observed.
Geotti-Bianchini Piero, Crisma Marco, Peggion Cristina, Bianco Alberto, Formaggio Fernando
Synthesis and 3D-structure of conformationally controlled nucleo-peptides Journal Article
In: Advances in Experimental Medicine and Biology, vol. 611, pp. 37–38, 2009, ISSN: 0065-2598.
Links | BibTeX | Tags: I2CT, Models, Molecular, Nucleoproteins, Peptides, Protein Conformation, Team-Bianco
@article{geotti-bianchini_synthesis_2009,
title = {Synthesis and 3D-structure of conformationally controlled nucleo-peptides},
author = {Piero Geotti-Bianchini and Marco Crisma and Cristina Peggion and Alberto Bianco and Fernando Formaggio},
doi = {10.1007/978-0-387-73657-0_16},
issn = {0065-2598},
year = {2009},
date = {2009-01-01},
journal = {Advances in Experimental Medicine and Biology},
volume = {611},
pages = {37--38},
keywords = {I2CT, Models, Molecular, Nucleoproteins, Peptides, Protein Conformation, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2008
Yandar Nubia, Pastorin Giorgia, Prato Maurizio, Bianco Alberto, Patarroyo Manuel Elkin, Lozano José Manuel
Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model Journal Article
In: Vaccine, vol. 26, no. 46, pp. 5864–5873, 2008, ISSN: 0264-410X.
Abstract | Links | BibTeX | Tags: Apical membrane antigen 1 (AMA-1), Functionalized carbon nanotubes (-CNT), I2CT, Synthetic vaccine delivery system, Team-Bianco
@article{yandar_immunological_2008,
title = {Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model},
author = {Nubia Yandar and Giorgia Pastorin and Maurizio Prato and Alberto Bianco and Manuel Elkin Patarroyo and José Manuel Lozano},
url = {http://www.sciencedirect.com/science/article/pii/S0264410X08010736},
doi = {10.1016/j.vaccine.2008.08.014},
issn = {0264-410X},
year = {2008},
date = {2008-10-01},
urldate = {2020-03-31},
journal = {Vaccine},
volume = {26},
number = {46},
pages = {5864--5873},
abstract = {We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated to CNTs as well as being recognized by BALB/c polyclonal antibodies. Subsequent experiments lead us to assess the AMA-1 peptide alone, as well as the CNT-peptide conjugate regarding rodent malarial infection. Remarkably, the peptide effectively controlled and delayed Plasmodium berghei-challenged animals’ parasitaemia. The peptide-CNT conjugate displayed similar immunological properties to the peptide alone by protecting or delaying malarial infection. The peptide presentation by f-CNT to the immune system thus constitutes a promising approach for synthetic malarial vaccine formulation since the immunogen peptide conformation is well preserved.},
keywords = {Apical membrane antigen 1 (AMA-1), Functionalized carbon nanotubes (-CNT), I2CT, Synthetic vaccine delivery system, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated to CNTs as well as being recognized by BALB/c polyclonal antibodies. Subsequent experiments lead us to assess the AMA-1 peptide alone, as well as the CNT-peptide conjugate regarding rodent malarial infection. Remarkably, the peptide effectively controlled and delayed Plasmodium berghei-challenged animals’ parasitaemia. The peptide-CNT conjugate displayed similar immunological properties to the peptide alone by protecting or delaying malarial infection. The peptide presentation by f-CNT to the immune system thus constitutes a promising approach for synthetic malarial vaccine formulation since the immunogen peptide conformation is well preserved.
Geotti-Bianchini Piero, Beyrath Julien, Chaloin Olivier, Formaggio Fernando, Bianco Alberto
Design and synthesis of intrinsically cell-penetrating nucleopeptides Journal Article
In: Organic & Biomolecular Chemistry, vol. 6, no. 20, pp. 3661–3663, 2008, ISSN: 1477-0539.
Abstract | Links | BibTeX | Tags: Amino Acid Sequence, Cell Line, Cells, Drug Design, Humans, I2CT, Molecular Sequence Data, Peptides, Purines, Pyrimidines, Team-Bianco
@article{geotti-bianchini_design_2008,
title = {Design and synthesis of intrinsically cell-penetrating nucleopeptides},
author = {Piero Geotti-Bianchini and Julien Beyrath and Olivier Chaloin and Fernando Formaggio and Alberto Bianco},
doi = {10.1039/b811639c},
issn = {1477-0539},
year = {2008},
date = {2008-10-01},
journal = {Organic & Biomolecular Chemistry},
volume = {6},
number = {20},
pages = {3661--3663},
abstract = {Nucleopeptides, which are constituted of alpha-amino acids bearing nucleobases at their side chains, are able to penetrate into cells and to reach the nucleus without cytotoxic effects.},
keywords = {Amino Acid Sequence, Cell Line, Cells, Drug Design, Humans, I2CT, Molecular Sequence Data, Peptides, Purines, Pyrimidines, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Nucleopeptides, which are constituted of alpha-amino acids bearing nucleobases at their side chains, are able to penetrate into cells and to reach the nucleus without cytotoxic effects.
Lacerda Lara, Herrero Maria A, Venner Kerrie, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon-nanotube shape and individualization critical for renal excretion Journal Article
In: Small (Weinheim an Der Bergstrasse, Germany), vol. 4, no. 8, pp. 1130–1132, 2008, ISSN: 1613-6829.
Links | BibTeX | Tags: Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission
@article{lacerda_carbon-nanotube_2008,
title = {Carbon-nanotube shape and individualization critical for renal excretion},
author = {Lara Lacerda and Maria A Herrero and Kerrie Venner and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200800323},
issn = {1613-6829},
year = {2008},
date = {2008-08-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {4},
number = {8},
pages = {1130--1132},
keywords = {Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
Fabre Bruno, Hauquier Fanny, Herrier Cyril, Pastorin Giorgia, Wu Wei, Bianco Alberto, Prato Maurizio, Hapiot Philippe, Zigah Dodzi, Prasciolu Mauro, Vaccari Lisa
Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces Journal Article
In: Langmuir: the ACS journal of surfaces and colloids, vol. 24, no. 13, pp. 6595–6602, 2008, ISSN: 0743-7463.
Abstract | Links | BibTeX | Tags: Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco
@article{fabre_covalent_2008,
title = {Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces},
author = {Bruno Fabre and Fanny Hauquier and Cyril Herrier and Giorgia Pastorin and Wei Wu and Alberto Bianco and Maurizio Prato and Philippe Hapiot and Dodzi Zigah and Mauro Prasciolu and Lisa Vaccari},
doi = {10.1021/la800358w},
issn = {0743-7463},
year = {2008},
date = {2008-06-01},
journal = {Langmuir: the ACS journal of surfaces and colloids},
volume = {24},
number = {13},
pages = {6595--6602},
abstract = {Multiwalled carbon nanotubes (MWNTs) covalently bound to monocrystalline p-type Si(111) surfaces have been prepared by attaching soluble amine-functionalized MWNTs onto a preassembled undecanoic acid monolayer using carbodiimide coupling. SEM analysis of these functionalized surfaces shows that the bound MWNTs are parallel to the surface rather than perpendicular. The voltammetric and electrochemical impedance spectroscopy measurements reveal that the electron transfer at the MWNT-modified surface is faster than that observed at a MWNT-free alkyl monolayer. We have also demonstrated that it is possible to prepare MWNT micropatterns using this surface amidation reaction and a "reagentless" UV photolithography technique. Following this approach, MWNT patterns surrounded by n-dodecyl areas have been produced and the local electrochemical properties of these micropatterned surfaces have been examined by scanning electrochemical microscopy. In particular, it is demonstrated that the MWNT patterns allow a faster charge transfer which is consistent with the results obtained for the uniformly modified surfaces.},
keywords = {Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Multiwalled carbon nanotubes (MWNTs) covalently bound to monocrystalline p-type Si(111) surfaces have been prepared by attaching soluble amine-functionalized MWNTs onto a preassembled undecanoic acid monolayer using carbodiimide coupling. SEM analysis of these functionalized surfaces shows that the bound MWNTs are parallel to the surface rather than perpendicular. The voltammetric and electrochemical impedance spectroscopy measurements reveal that the electron transfer at the MWNT-modified surface is faster than that observed at a MWNT-free alkyl monolayer. We have also demonstrated that it is possible to prepare MWNT micropatterns using this surface amidation reaction and a "reagentless" UV photolithography technique. Following this approach, MWNT patterns surrounded by n-dodecyl areas have been produced and the local electrochemical properties of these micropatterned surfaces have been examined by scanning electrochemical microscopy. In particular, it is demonstrated that the MWNT patterns allow a faster charge transfer which is consistent with the results obtained for the uniformly modified surfaces.
Li Shouping, Wu Wei, Campidelli Stéphane, Sarnatskaïa Veronika, Prato Maurizio, Tridon Arlette, Nikolaev Andrey, Nikolaev Vladimir, Bianco Alberto, Snezhkova Elisaveta
Adsorption of carbon nanotubes on active carbon microparticles Journal Article
In: Carbon, vol. 46, no. 7, pp. 1091–1095, 2008, ISSN: 0008-6223.
Links | BibTeX | Tags: I2CT, Team-Bianco
@article{li_adsorption_2008,
title = {Adsorption of carbon nanotubes on active carbon microparticles},
author = {Shouping Li and Wei Wu and Stéphane Campidelli and Veronika Sarnatskaïa and Maurizio Prato and Arlette Tridon and Andrey Nikolaev and Vladimir Nikolaev and Alberto Bianco and Elisaveta Snezhkova},
url = {http://www.sciencedirect.com/science/article/pii/S0008622308001462},
doi = {10.1016/j.carbon.2008.03.010},
issn = {0008-6223},
year = {2008},
date = {2008-06-01},
urldate = {2020-03-31},
journal = {Carbon},
volume = {46},
number = {7},
pages = {1091--1095},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Kostarelos Kostas, Bianco Alberto, Prato Maurizio
Hype around nanotubes creates unrealistic hopes Journal Article
In: Nature, vol. 453, no. 7193, pp. 280, 2008, ISSN: 1476-4687.
Links | BibTeX | Tags: Adult Stem Cells, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Reproducibility of Results, Team-Bianco
@article{kostarelos_hype_2008,
title = {Hype around nanotubes creates unrealistic hopes},
author = {Kostas Kostarelos and Alberto Bianco and Maurizio Prato},
doi = {10.1038/453280c},
issn = {1476-4687},
year = {2008},
date = {2008-05-01},
journal = {Nature},
volume = {453},
number = {7193},
pages = {280},
keywords = {Adult Stem Cells, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Reproducibility of Results, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Lacerda Lara, Ali-Boucetta Hanene, Herrero Maria A, Pastorin Giorgia, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Tissue histology and physiology following intravenous administration of different types of functionalized multiwalled carbon nanotubes Journal Article
In: Nanomedicine (London, England), vol. 3, no. 2, pp. 149–161, 2008, ISSN: 1748-6963.
Abstract | Links | BibTeX | Tags: Animals, carbon, Female, I2CT, Inbred BALB C, Injections, Intravenous, Mice, Nanotubes, Organ Specificity, Team-Bianco, Tissue Distribution
@article{lacerda_tissue_2008,
title = {Tissue histology and physiology following intravenous administration of different types of functionalized multiwalled carbon nanotubes},
author = {Lara Lacerda and Hanene Ali-Boucetta and Maria A Herrero and Giorgia Pastorin and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.2217/17435889.3.2.149},
issn = {1748-6963},
year = {2008},
date = {2008-04-01},
journal = {Nanomedicine (London, England)},
volume = {3},
number = {2},
pages = {149--161},
abstract = {BACKGROUND: Carbon nanotubes (CNTs) constitute one of the most important types of nanomaterials, increasingly gaining interest as tools for nanomedicine applications, such as sensors, implants or delivery systems. Our groups have reported previously that chemical functionalization of CNTs can lead to their almost complete elimination from the body of animals through the urinary excretion route. The administration of CNTs may, however, impact the physiological function of organs through which CNTs traverse or accumulate. AIM: The present study addresses the short-term impact (first 24 h) of intravenous administration of various types of multiwalled nanotubes (MWNTs) on the physiology of healthy mice. MATERIALS & METHODS: Nonfunctionalized, purified MWNTs (pMWNTs) and different types of water-dispersible, functionalized MWNTs (f-MWNTs) were tail-vein injected. Histological examination of tissues (kidney, liver, spleen and lung) harvested 24 h post-administration indicated that organ accumulation depended on the degree of ammonium (NH(3)(+)) functionalization at the f-MWNT surface. RESULTS: The higher the degree of functionalization of MWNT-NH(3)(+), the less their accumulation in tissues. pMWNTs coated with autologous serum proteins prior to injection accumulated almost entirely in the lung and liver in large dark clusters. Moreover, various indicators of serum and urine analyses also confirmed that MWNT-NH(3)(+) injections did not induce any physiological abnormality in all major organs within the first 24 h post-injection. Interestingly, no abnormalities were observed either for f-MWNTs highly functionalized with carboxylate groups (diethylentriaminepentaacetic-functionalized MWNTs) or by upscaling to the highest doses ever injected so far in vivo (20 mg/kg). CONCLUSION: The high degree of f-MWNT functionalization responsible for adequate individualization of nanotubes and not the nature of the functional groups was the critical factor leading to less tissue accumulation and normal tissue physiology at least within the first 24 h post-administration, even at the highest carbon nanotube doses ever administered in any study today.},
keywords = {Animals, carbon, Female, I2CT, Inbred BALB C, Injections, Intravenous, Mice, Nanotubes, Organ Specificity, Team-Bianco, Tissue Distribution},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Carbon nanotubes (CNTs) constitute one of the most important types of nanomaterials, increasingly gaining interest as tools for nanomedicine applications, such as sensors, implants or delivery systems. Our groups have reported previously that chemical functionalization of CNTs can lead to their almost complete elimination from the body of animals through the urinary excretion route. The administration of CNTs may, however, impact the physiological function of organs through which CNTs traverse or accumulate. AIM: The present study addresses the short-term impact (first 24 h) of intravenous administration of various types of multiwalled nanotubes (MWNTs) on the physiology of healthy mice. MATERIALS & METHODS: Nonfunctionalized, purified MWNTs (pMWNTs) and different types of water-dispersible, functionalized MWNTs (f-MWNTs) were tail-vein injected. Histological examination of tissues (kidney, liver, spleen and lung) harvested 24 h post-administration indicated that organ accumulation depended on the degree of ammonium (NH(3)(+)) functionalization at the f-MWNT surface. RESULTS: The higher the degree of functionalization of MWNT-NH(3)(+), the less their accumulation in tissues. pMWNTs coated with autologous serum proteins prior to injection accumulated almost entirely in the lung and liver in large dark clusters. Moreover, various indicators of serum and urine analyses also confirmed that MWNT-NH(3)(+) injections did not induce any physiological abnormality in all major organs within the first 24 h post-injection. Interestingly, no abnormalities were observed either for f-MWNTs highly functionalized with carboxylate groups (diethylentriaminepentaacetic-functionalized MWNTs) or by upscaling to the highest doses ever injected so far in vivo (20 mg/kg). CONCLUSION: The high degree of f-MWNT functionalization responsible for adequate individualization of nanotubes and not the nature of the functional groups was the critical factor leading to less tissue accumulation and normal tissue physiology at least within the first 24 h post-administration, even at the highest carbon nanotube doses ever administered in any study today.
Ali-Boucetta Hanene, Al-Jamal Khuloud T, McCarthy David, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Multiwalled carbon nanotube-doxorubicin supramolecular complexes for cancer therapeutics Journal Article
In: Chemical Communications (Cambridge, England), no. 4, pp. 459–461, 2008, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: Antineoplastic Agents, Breast Neoplasms, carbon, Cultured, Doxorubicin, Electron, Humans, I2CT, Microscopy, Nanotubes, Team-Bianco, Transmission, Tumor Cells
@article{ali-boucetta_multiwalled_2008,
title = {Multiwalled carbon nanotube-doxorubicin supramolecular complexes for cancer therapeutics},
author = {Hanene Ali-Boucetta and Khuloud T Al-Jamal and David McCarthy and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1039/b712350g},
issn = {1359-7345},
year = {2008},
date = {2008-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {4},
pages = {459--461},
abstract = {Multiwalled carbon nanotube aqueous dispersions using block copolymers are able to form supramolecular complexes with the aromatic chromophore and anticancer agent doxorubicin via pi-pi stacking and enhance its cytotoxic activity.},
keywords = {Antineoplastic Agents, Breast Neoplasms, carbon, Cultured, Doxorubicin, Electron, Humans, I2CT, Microscopy, Nanotubes, Team-Bianco, Transmission, Tumor Cells},
pubstate = {published},
tppubtype = {article}
}
Multiwalled carbon nanotube aqueous dispersions using block copolymers are able to form supramolecular complexes with the aromatic chromophore and anticancer agent doxorubicin via pi-pi stacking and enhance its cytotoxic activity.
Prato Maurizio, Kostarelos Kostas, Bianco Alberto
Functionalized carbon nanotubes in drug design and discovery Journal Article
In: Accounts of Chemical Research, vol. 41, no. 1, pp. 60–68, 2008, ISSN: 1520-4898.
Abstract | Links | BibTeX | Tags: Animals, carbon, Communicable Diseases, Drug Carriers, Drug Design, Genetic Therapy, Humans, I2CT, Immunization, Nanotubes, Neoplasms, Team-Bianco
@article{prato_functionalized_2008,
title = {Functionalized carbon nanotubes in drug design and discovery},
author = {Maurizio Prato and Kostas Kostarelos and Alberto Bianco},
doi = {10.1021/ar700089b},
issn = {1520-4898},
year = {2008},
date = {2008-01-01},
journal = {Accounts of Chemical Research},
volume = {41},
number = {1},
pages = {60--68},
abstract = {Carbon nanotubes (CNTs) have been proposed and actively explored as multipurpose innovative carriers for drug delivery and diagnostic applications. Their versatile physicochemical features enable the covalent and noncovalent introduction of several pharmaceutically relevant entities and allow for rational design of novel candidate nanoscale constructs for drug development. CNTs can be functionalized with different functional groups to carry simultaneously several moieties for targeting, imaging, and therapy. Among the most interesting examples of such multimodal CNT constructs described in this Account is one carrying a fluorescein probe together with the antifungal drug amphotericin B or fluorescein and the antitumor agent methotrexate. The biological action of the drug in these cases is retained or, as in the case of amphotericin B constructs, enhanced, while CNTs are able to reduce the unwanted toxicity of the drug administered alone. Ammonium-functionalized CNTs can also be considered very promising vectors for gene-encoding nucleic acids. Indeed, we have formed stable complexes between cationic CNTs and plasmid DNA and demonstrated the enhancement of the gene therapeutic capacity in comparison to DNA alone. On the other hand, CNTs conjugated with antigenic peptides can be developed as a new and effective system for synthetic vaccine applications. What makes CNTs quite unique is their ability, first shown by our groups in 2004, to passively cross membranes of many different types of cells following a translocation mechanism that has been termed the nanoneedle mechanism. In that way, CNTs open innumerable possibilities for future drug discovery based on intracellular targets that have been hard to reach until today. Moreover, adequately functionalized CNTs as those shown in this Account can be rapidly eliminated from the body following systemic administration offering further encouragment for their development. CNT excretion rates and accumulation in organs and any reactivity with the immune system will determine the CNT safety profile and, consequently, any further pharmaceutical development. Caution is advised about the need for systematic data on the long-term fate of these very interesting and versatile nano-objects in correlation with the type of CNT material used. CNTs are gradually plyaing a bigger and more important role in the emerging field of nanomedicine; however, we need to guarantee that the great opportunities they offer will be translated into feasible and safe constructs to be included in drug discovery and development pipelines.},
keywords = {Animals, carbon, Communicable Diseases, Drug Carriers, Drug Design, Genetic Therapy, Humans, I2CT, Immunization, Nanotubes, Neoplasms, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) have been proposed and actively explored as multipurpose innovative carriers for drug delivery and diagnostic applications. Their versatile physicochemical features enable the covalent and noncovalent introduction of several pharmaceutically relevant entities and allow for rational design of novel candidate nanoscale constructs for drug development. CNTs can be functionalized with different functional groups to carry simultaneously several moieties for targeting, imaging, and therapy. Among the most interesting examples of such multimodal CNT constructs described in this Account is one carrying a fluorescein probe together with the antifungal drug amphotericin B or fluorescein and the antitumor agent methotrexate. The biological action of the drug in these cases is retained or, as in the case of amphotericin B constructs, enhanced, while CNTs are able to reduce the unwanted toxicity of the drug administered alone. Ammonium-functionalized CNTs can also be considered very promising vectors for gene-encoding nucleic acids. Indeed, we have formed stable complexes between cationic CNTs and plasmid DNA and demonstrated the enhancement of the gene therapeutic capacity in comparison to DNA alone. On the other hand, CNTs conjugated with antigenic peptides can be developed as a new and effective system for synthetic vaccine applications. What makes CNTs quite unique is their ability, first shown by our groups in 2004, to passively cross membranes of many different types of cells following a translocation mechanism that has been termed the nanoneedle mechanism. In that way, CNTs open innumerable possibilities for future drug discovery based on intracellular targets that have been hard to reach until today. Moreover, adequately functionalized CNTs as those shown in this Account can be rapidly eliminated from the body following systemic administration offering further encouragment for their development. CNT excretion rates and accumulation in organs and any reactivity with the immune system will determine the CNT safety profile and, consequently, any further pharmaceutical development. Caution is advised about the need for systematic data on the long-term fate of these very interesting and versatile nano-objects in correlation with the type of CNT material used. CNTs are gradually plyaing a bigger and more important role in the emerging field of nanomedicine; however, we need to guarantee that the great opportunities they offer will be translated into feasible and safe constructs to be included in drug discovery and development pipelines.
Lacerda L, Soundararajan A, Singh R, Pastorin G, Al‐Jamal K T, Turton J, Frederik P, Herrero M A, Li S, Bao A, Emfietzoglou D, Mather S, Phillips W T, Prato M, Bianco A, Goins B, Kostarelos K
Dynamic Imaging of Functionalized Multi-Walled Carbon Nanotube Systemic Circulation and Urinary Excretion Journal Article
In: Advanced Materials, vol. 20, no. 2, pp. 225–230, 2008, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: Biomedical applications, Carbon nanotubes, I2CT, multiwalled, Nanomaterials, Team-Bianco
@article{lacerda_dynamic_2008,
title = {Dynamic Imaging of Functionalized Multi-Walled Carbon Nanotube Systemic Circulation and Urinary Excretion},
author = {L Lacerda and A Soundararajan and R Singh and G Pastorin and K T Al‐Jamal and J Turton and P Frederik and M A Herrero and S Li and A Bao and D Emfietzoglou and S Mather and W T Phillips and M Prato and A Bianco and B Goins and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200702334},
doi = {10.1002/adma.200702334},
issn = {1521-4095},
year = {2008},
date = {2008-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {20},
number = {2},
pages = {225--230},
abstract = {Intravenously administered multi-walled carbon nanotubes, functionalized with DTPA and radiolabeled with Indium-111, were dynamically tracked in vivo using a microSingle Photon Emission Tomography scanner. Imaging showed that nanotubes enter the systemic blood circulation and within 5 min begin to permeate through the renal glomerular filtration system into the bladder.},
keywords = {Biomedical applications, Carbon nanotubes, I2CT, multiwalled, Nanomaterials, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Intravenously administered multi-walled carbon nanotubes, functionalized with DTPA and radiolabeled with Indium-111, were dynamically tracked in vivo using a microSingle Photon Emission Tomography scanner. Imaging showed that nanotubes enter the systemic blood circulation and within 5 min begin to permeate through the renal glomerular filtration system into the bladder.
Lacotte Stéphanie, García Ainara, Décossas Marion, Al‐Jamal Wafa' T, Li Shouping, Kostarelos Kostas, Muller Sylviane, Prato Maurizio, Dumortier Hélène, Bianco Alberto
Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells Journal Article
In: Advanced Materials, vol. 20, no. 12, pp. 2421–2426, 2008, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: carbon nanostructures, Cells, Cytotoxicity, Drug delivery, I2CT, Team-Bianco
@article{lacotte_interfacing_2008,
title = {Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells},
author = {Stéphanie Lacotte and Ainara García and Marion Décossas and Wafa' T Al‐Jamal and Shouping Li and Kostas Kostarelos and Sylviane Muller and Maurizio Prato and Hélène Dumortier and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200702753},
doi = {10.1002/adma.200702753},
issn = {1521-4095},
year = {2008},
date = {2008-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {20},
number = {12},
pages = {2421--2426},
abstract = {Functionalized carbon nanohorns (f-CNH) are uptaken by macrophages, without affecting cell viability. f-CNH induce the production of reactive oxygen species and of pro-inflammatory cytokines. The level of inflammation, although moderate, should be taken into consideration when using f-CNH for drug delivery. However, it could be exploited as an intrinsic f-CNH adjuvant function for biomedical applications requiring some activation of the immune system.},
keywords = {carbon nanostructures, Cells, Cytotoxicity, Drug delivery, I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalized carbon nanohorns (f-CNH) are uptaken by macrophages, without affecting cell viability. f-CNH induce the production of reactive oxygen species and of pro-inflammatory cytokines. The level of inflammation, although moderate, should be taken into consideration when using f-CNH for drug delivery. However, it could be exploited as an intrinsic f-CNH adjuvant function for biomedical applications requiring some activation of the immune system.
Violette Aude, Lancelot Nathalie, Poschalko Alexander, Piotto Martial, Briand Jean-Paul, Raya Jesus, Elbayed Karim, Bianco Alberto, Guichard Gilles
Exploring helical folding of oligoureas during chain elongation by high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy Journal Article
In: Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 14, no. 13, pp. 3874–3882, 2008, ISSN: 0947-6539.
Abstract | Links | BibTeX | Tags: I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Solvents, Team-Bianco, Urea
@article{violette_exploring_2008,
title = {Exploring helical folding of oligoureas during chain elongation by high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy},
author = {Aude Violette and Nathalie Lancelot and Alexander Poschalko and Martial Piotto and Jean-Paul Briand and Jesus Raya and Karim Elbayed and Alberto Bianco and Gilles Guichard},
doi = {10.1002/chem.200701923},
issn = {0947-6539},
year = {2008},
date = {2008-01-01},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
volume = {14},
number = {13},
pages = {3874--3882},
abstract = {The development of novel folding oligomers (foldamers) for biological and biomedical applications requires both precise structural information and appropriate methods to detect folding propensity. However, the synthesis and the systematic conformational investigation of large arrays of oligomers to determine the influence of factors, such as chain length, side chains, and surrounding environment, on secondary structure can be quite tedious. Herein, we show for 2.5-helical N,N'-linked oligoureas (gamma-peptide lineage) that the whole process of foldamer characterization can be accelerated by using high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy. This was achieved by monitoring a simple descriptor of conformational homogeneity (e.g., chemical shift difference between diastereotopic main chain CH2 protons) at different stages of oligourea chain growth on a solid support. HRMAS NMR experiments were conducted on two sets of oligoureas, ranging from dimer to hexamer, immobilized on DEUSS, a perdeuterated poly(oxyethylene)-based solid support swollen in solvents of low to high polarity. One evident advantage of the method is that only minute amount of material is required. In addition, the resonance of the deuterated resin is almost negligeable. On-bead NOESY spectra of high quality and with resolution comparable to that of liquid samples were obtained for longer oligomers, thus allowing detailed structural characterization.},
keywords = {I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Solvents, Team-Bianco, Urea},
pubstate = {published},
tppubtype = {article}
}
The development of novel folding oligomers (foldamers) for biological and biomedical applications requires both precise structural information and appropriate methods to detect folding propensity. However, the synthesis and the systematic conformational investigation of large arrays of oligomers to determine the influence of factors, such as chain length, side chains, and surrounding environment, on secondary structure can be quite tedious. Herein, we show for 2.5-helical N,N'-linked oligoureas (gamma-peptide lineage) that the whole process of foldamer characterization can be accelerated by using high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy. This was achieved by monitoring a simple descriptor of conformational homogeneity (e.g., chemical shift difference between diastereotopic main chain CH2 protons) at different stages of oligourea chain growth on a solid support. HRMAS NMR experiments were conducted on two sets of oligoureas, ranging from dimer to hexamer, immobilized on DEUSS, a perdeuterated poly(oxyethylene)-based solid support swollen in solvents of low to high polarity. One evident advantage of the method is that only minute amount of material is required. In addition, the resonance of the deuterated resin is almost negligeable. On-bead NOESY spectra of high quality and with resolution comparable to that of liquid samples were obtained for longer oligomers, thus allowing detailed structural characterization.
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Opportunities and challenges of carbon-based nanomaterials for cancer therapy Journal Article
In: Expert Opinion on Drug Delivery, vol. 5, no. 3, pp. 331–342, 2008, ISSN: 1742-5247.
Abstract | Links | BibTeX | Tags: Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission
@article{bianco_opportunities_2008,
title = {Opportunities and challenges of carbon-based nanomaterials for cancer therapy},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1517/17425247.5.3.331},
issn = {1742-5247},
year = {2008},
date = {2008-01-01},
journal = {Expert Opinion on Drug Delivery},
volume = {5},
number = {3},
pages = {331--342},
abstract = {The possibility of incorporating carbon-based nanomaterials into living systems has opened the way for the investigation of their potential applications in the emerging field of nanomedicine. A wide variety of different nanomaterials based on allotropic forms of carbon, such as nanotubes, nanohorns and nanodiamonds, are currently being explored towards different biomedical applications. In this review, we discuss the recent advances in the development of these novel nanomaterials for cancer therapy. A comparison between the characteristics, the advantages, the drawbacks, the benefits and the risks associated with these novel biocompatible forms of carbon is presented here.},
keywords = {Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission},
pubstate = {published},
tppubtype = {article}
}
The possibility of incorporating carbon-based nanomaterials into living systems has opened the way for the investigation of their potential applications in the emerging field of nanomedicine. A wide variety of different nanomaterials based on allotropic forms of carbon, such as nanotubes, nanohorns and nanodiamonds, are currently being explored towards different biomedical applications. In this review, we discuss the recent advances in the development of these novel nanomaterials for cancer therapy. A comparison between the characteristics, the advantages, the drawbacks, the benefits and the risks associated with these novel biocompatible forms of carbon is presented here.
2007
Lacerda Lara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon nanotube cell translocation and delivery of nucleic acidsin vitro and in vivo Journal Article
In: Journal of Materials Chemistry, vol. 18, no. 1, pp. 17–22, 2007, ISSN: 1364-5501.
Abstract | Links | BibTeX | Tags: I2CT, Team-Bianco
@article{lacerda_carbon_2007,
title = {Carbon nanotube cell translocation and delivery of nucleic acidsin vitro and in vivo},
author = {Lara Lacerda and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
url = {https://pubs.rsc.org/en/content/articlelanding/2008/jm/b711554g},
doi = {10.1039/B711554G},
issn = {1364-5501},
year = {2007},
date = {2007-12-01},
urldate = {2020-03-31},
journal = {Journal of Materials Chemistry},
volume = {18},
number = {1},
pages = {17--22},
abstract = {In the last few years, the carbon nanotube (CNT) field has seen a new direction of investigation growing rapidly, along with the interest of more researchers from diverse fields of expertise interested in this new material in an attempt to exploit their properties in biomedical applications. Here we describe the most recently reported work on the application of CNT for gene encoding nucleic acid (DNA and RNA) delivery purposes by using in vitro and in vivo models. Several groups have now successfully observed the cellular internalisation of nucleic acids with the aid of CNT following very different protocols. The main processes for the internalisation pathways and intracellular release of the nucleic acids are here reviewed. Furthermore, we have just started to see some initial studies of in vivo work using siRNA-CNT conjugates to achieve silencing in tumour tissue. Admittedly, it is still very early days for the technology, but future studies are necessary, and will surely appear, in order to determine the feasibility of bringing the CNT closer to the clinic.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
In the last few years, the carbon nanotube (CNT) field has seen a new direction of investigation growing rapidly, along with the interest of more researchers from diverse fields of expertise interested in this new material in an attempt to exploit their properties in biomedical applications. Here we describe the most recently reported work on the application of CNT for gene encoding nucleic acid (DNA and RNA) delivery purposes by using in vitro and in vivo models. Several groups have now successfully observed the cellular internalisation of nucleic acids with the aid of CNT following very different protocols. The main processes for the internalisation pathways and intracellular release of the nucleic acids are here reviewed. Furthermore, we have just started to see some initial studies of in vivo work using siRNA-CNT conjugates to achieve silencing in tumour tissue. Admittedly, it is still very early days for the technology, but future studies are necessary, and will surely appear, in order to determine the feasibility of bringing the CNT closer to the clinic.
Lacerda Lara, Raffa Simona, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Cell-penetrating CNTs for delivery of therapeutics Journal Article
In: Nano Today, vol. 2, no. 6, pp. 38–43, 2007, ISSN: 1748-0132.
Abstract | Links | BibTeX | Tags: I2CT, Team-Bianco
@article{lacerda_cell-penetrating_2007,
title = {Cell-penetrating CNTs for delivery of therapeutics},
author = {Lara Lacerda and Simona Raffa and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
url = {http://www.sciencedirect.com/science/article/pii/S174801320770172X},
doi = {10.1016/S1748-0132(07)70172-X},
issn = {1748-0132},
year = {2007},
date = {2007-12-01},
urldate = {2020-03-31},
journal = {Nano Today},
volume = {2},
number = {6},
pages = {38--43},
abstract = {The use of carbon-based nanostructures, such as carbon nanotubes, in biomedicine is increasingly attracting attention. One key advantage of carbon nanotubes is their ability to translocate through plasma membranes, allowing their use for the delivery of therapeutically active molecules in a manner that resembles cell-penetrating peptides. Moreover, exploitation of their unique electrical, optical, thermal, and spectroscopic properties in a biological context is hoped to yield great advances in the detection, monitoring, and therapy of disease. Here we offer a speculative overview of the general principles behind the mechanism of carbon nanotube penetration of the plasma membrane and a snapshot of the different therapeutic modalities based on these fascinating nanostructures that are currently being investigated.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The use of carbon-based nanostructures, such as carbon nanotubes, in biomedicine is increasingly attracting attention. One key advantage of carbon nanotubes is their ability to translocate through plasma membranes, allowing their use for the delivery of therapeutically active molecules in a manner that resembles cell-penetrating peptides. Moreover, exploitation of their unique electrical, optical, thermal, and spectroscopic properties in a biological context is hoped to yield great advances in the detection, monitoring, and therapy of disease. Here we offer a speculative overview of the general principles behind the mechanism of carbon nanotube penetration of the plasma membrane and a snapshot of the different therapeutic modalities based on these fascinating nanostructures that are currently being investigated.
Habib Mohammed, Rivas Magali Noval, Chamekh Mustapha, Wieckowski Sébastien, Sun Weimin, Bianco Alberto, Trouche Nathalie, Chaloin Olivier, Dumortier Hélène, Goldman Michel, Guichard Gilles, Fournel Sylvie, Vray Bernard
In: The Journal of Immunology, vol. 178, no. 11, pp. 6700–6704, 2007, ISSN: 0022-1767, 1550-6606.
Abstract | Links | BibTeX | Tags: I2CT, Team-Bianco
@article{habib_cutting_2007b,
title = {Cutting Edge: Small Molecule CD40 Ligand Mimetics Promote Control of Parasitemia and Enhance Ŧ Cells Producing IFN-γ during Experimental Trypanosoma cruzi Infection},
author = {Mohammed Habib and Magali Noval Rivas and Mustapha Chamekh and Sébastien Wieckowski and Weimin Sun and Alberto Bianco and Nathalie Trouche and Olivier Chaloin and Hélène Dumortier and Michel Goldman and Gilles Guichard and Sylvie Fournel and Bernard Vray},
url = {https://www.jimmunol.org/content/178/11/6700},
doi = {10.4049/jimmunol.178.11.6700},
issn = {0022-1767, 1550-6606},
year = {2007},
date = {2007-06-01},
urldate = {2020-03-31},
journal = {The Journal of Immunology},
volume = {178},
number = {11},
pages = {6700--6704},
abstract = {Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-γ. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textbackslashtextless3 kDa) mimicking trimeric CD40L (mini CD40Ls-1 and -2) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8+ T cells, and IFN-γ production. Mice surviving T. cruzi infection in the presence of miniCD40L-1 were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-γ. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textbackslashtextless3 kDa) mimicking trimeric CD40L (mini CD40Ls-1 and -2) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8+ T cells, and IFN-γ production. Mice surviving T. cruzi infection in the presence of miniCD40L-1 were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.
Habib Mohammed, Rivas Magali Noval, Chamekh Mustapha, Wieckowski Sébastien, Sun Weimin, Bianco Alberto, Trouche Nathalie, Chaloin Olivier, Dumortier Hélène, Goldman Michel, Guichard Gilles, Fournel Sylvie, Vray Bernard
In: Journal of Immunology (Baltimore, Md.: 1950), vol. 178, no. 11, pp. 6700–6704, 2007, ISSN: 0022-1767.
Abstract | Links | BibTeX | Tags: Animals, CD40 Antigens, CD40 Ligand, Cell Line, Cells, Chagas Disease, Cultured, Dumortier, I2CT, Inbred BALB C, Inbred C57BL, Interferon-gamma, Knockout, Mice, Molecular Mimicry, Parasitemia, T-Lymphocyte Subsets, Team-Bianco, Team-Dumortier, Trypanosoma cruzi
@article{habib_cutting_2007,
title = {Cutting edge: small molecule CD40 ligand mimetics promote control of parasitemia and enhance Ŧ cells producing IFN-gamma during experimental Trypanosoma cruzi infection},
author = {Mohammed Habib and Magali Noval Rivas and Mustapha Chamekh and Sébastien Wieckowski and Weimin Sun and Alberto Bianco and Nathalie Trouche and Olivier Chaloin and Hélène Dumortier and Michel Goldman and Gilles Guichard and Sylvie Fournel and Bernard Vray},
doi = {10.4049/jimmunol.178.11.6700},
issn = {0022-1767},
year = {2007},
date = {2007-06-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {178},
number = {11},
pages = {6700--6704},
abstract = {Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-gamma. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textless3 kDa) mimicking trimeric CD40L (mini CD40Ls(-1) and (-2)) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8(+) T cells, and IFN-gamma production. Mice surviving T. cruzi infection in the presence of miniCD40L(-1) were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.},
keywords = {Animals, CD40 Antigens, CD40 Ligand, Cell Line, Cells, Chagas Disease, Cultured, Dumortier, I2CT, Inbred BALB C, Inbred C57BL, Interferon-gamma, Knockout, Mice, Molecular Mimicry, Parasitemia, T-Lymphocyte Subsets, Team-Bianco, Team-Dumortier, Trypanosoma cruzi},
pubstate = {published},
tppubtype = {article}
}
Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-gamma. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textless3 kDa) mimicking trimeric CD40L (mini CD40Ls(-1) and (-2)) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8(+) T cells, and IFN-gamma production. Mice surviving T. cruzi infection in the presence of miniCD40L(-1) were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.
Kostarelos Kostas, Lacerda Lara, Pastorin Giorgia, Wu Wei, Wieckowski Sébastien, Luangsivilay Jacqueline, Godefroy Sylvie, Pantarotto Davide, Briand Jean-Paul, Muller Sylviane, Prato Maurizio, Bianco Alberto
Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type Journal Article
In: Nature Nanotechnology, vol. 2, no. 2, pp. 108–113, 2007, ISSN: 1748-3395.
Links | BibTeX | Tags: Animals, carbon, Cell Membrane, Cells, Cultured, Diffusion, Humans, I2CT, Nanotubes, Team-Bianco
@article{kostarelos_cellular_2007,
title = {Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type},
author = {Kostas Kostarelos and Lara Lacerda and Giorgia Pastorin and Wei Wu and Sébastien Wieckowski and Jacqueline Luangsivilay and Sylvie Godefroy and Davide Pantarotto and Jean-Paul Briand and Sylviane Muller and Maurizio Prato and Alberto Bianco},
doi = {10.1038/nnano.2006.209},
issn = {1748-3395},
year = {2007},
date = {2007-02-01},
journal = {Nature Nanotechnology},
volume = {2},
number = {2},
pages = {108--113},
keywords = {Animals, carbon, Cell Membrane, Cells, Cultured, Diffusion, Humans, I2CT, Nanotubes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Lacerda L, Pastorin G, Gathercole D, Buddle J, Prato M, Bianco A, Kostarelos K
Intracellular Trafficking of Carbon Nanotubes by Confocal Laser Scanning Microscopy Journal Article
In: Advanced Materials, vol. 19, no. 14, pp. 1789–1789, 2007, ISSN: 1521-4095.
Links | BibTeX | Tags: Biomedical materials, Cells, Drug delivery, fluorescence, I2CT, Nanotubes, single-walled, Team-Bianco
@article{lacerda_intracellular_2007,
title = {Intracellular Trafficking of Carbon Nanotubes by Confocal Laser Scanning Microscopy},
author = {L Lacerda and G Pastorin and D Gathercole and J Buddle and M Prato and A Bianco and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200790051},
doi = {10.1002/adma.200790051},
issn = {1521-4095},
year = {2007},
date = {2007-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {19},
number = {14},
pages = {1789--1789},
keywords = {Biomedical materials, Cells, Drug delivery, fluorescence, I2CT, Nanotubes, single-walled, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Klumpp Cédric, Lacerda Lara, Chaloin Olivier, Ros Tatiana Da, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Multifunctionalised cationic fullerene adducts for gene transfer: design, synthesis and DNA complexation Journal Article
In: Chemical Communications (Cambridge, England), no. 36, pp. 3762–3764, 2007, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: DNA, Electrophoresis, Fullerenes, Gene Transfer Techniques, I2CT, Molecular Structure, Plasmids, Team-Bianco
@article{klumpp_multifunctionalised_2007,
title = {Multifunctionalised cationic fullerene adducts for gene transfer: design, synthesis and DNA complexation},
author = {Cédric Klumpp and Lara Lacerda and Olivier Chaloin and Tatiana Da Ros and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b708435h},
issn = {1359-7345},
year = {2007},
date = {2007-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {36},
pages = {3762--3764},
abstract = {Cationic poly-N,N-dimethylfulleropyrrolidinium derivatives have been designed and synthesised to complex plasmid DNA for gene delivery.},
keywords = {DNA, Electrophoresis, Fullerenes, Gene Transfer Techniques, I2CT, Molecular Structure, Plasmids, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Cationic poly-N,N-dimethylfulleropyrrolidinium derivatives have been designed and synthesised to complex plasmid DNA for gene delivery.
2006
Lacerda Lara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon nanotubes as nanomedicines: from toxicology to pharmacology Journal Article
In: Advanced Drug Delivery Reviews, vol. 58, no. 14, pp. 1460–1470, 2006, ISSN: 0169-409X.
Abstract | Links | BibTeX | Tags: Animals, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Pharmacokinetics, Pharmacology, Team-Bianco
@article{lacerda_carbon_2006,
title = {Carbon nanotubes as nanomedicines: from toxicology to pharmacology},
author = {Lara Lacerda and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1016/j.addr.2006.09.015},
issn = {0169-409X},
year = {2006},
date = {2006-12-01},
journal = {Advanced Drug Delivery Reviews},
volume = {58},
number = {14},
pages = {1460--1470},
abstract = {Various biomedical applications of carbon nanotubes have been proposed in the last few years leading to the emergence of a new field in diagnostics and therapeutics. Most of these applications will involve the administration or implantation of carbon nanotubes and their matrices into patients. The toxicological and pharmacological profile of such carbon nanotube systems developed as nanomedicines will have to be determined prior to any clinical studies undertaken. This review brings together all the toxicological and pharmacological in vivo studies that have been carried out using carbon nanotubes, to offer the first summary of the state-of-the-art in the pharmaceutical development of carbon nanotubes on the road to becoming viable and effective nanomedicines.},
keywords = {Animals, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Pharmacokinetics, Pharmacology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Various biomedical applications of carbon nanotubes have been proposed in the last few years leading to the emergence of a new field in diagnostics and therapeutics. Most of these applications will involve the administration or implantation of carbon nanotubes and their matrices into patients. The toxicological and pharmacological profile of such carbon nanotube systems developed as nanomedicines will have to be determined prior to any clinical studies undertaken. This review brings together all the toxicological and pharmacological in vivo studies that have been carried out using carbon nanotubes, to offer the first summary of the state-of-the-art in the pharmaceutical development of carbon nanotubes on the road to becoming viable and effective nanomedicines.
Hauquier Fanny, Pastorin Giorgia, Hapiot Philippe, Prato Maurizio, Bianco Alberto, Fabre Bruno
Carbon nanotube-functionalized silicon surfaces with efficient redox communication Journal Article
In: Chemical Communications (Cambridge, England), no. 43, pp. 4536–4538, 2006, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: carbon, I2CT, Nanotubes, Oxidation-Reduction, Silicon, Surface Properties, Team-Bianco
@article{hauquier_carbon_2006,
title = {Carbon nanotube-functionalized silicon surfaces with efficient redox communication},
author = {Fanny Hauquier and Giorgia Pastorin and Philippe Hapiot and Maurizio Prato and Alberto Bianco and Bruno Fabre},
doi = {10.1039/b610559a},
issn = {1359-7345},
year = {2006},
date = {2006-11-01},
journal = {Chemical Communications (Cambridge, England)},
number = {43},
pages = {4536--4538},
abstract = {Sidewall functionalized multi-walled carbon nanotubes can be covalently bound parallel to a silicon surface via a self-assembled acid-terminated monolayer used as an organic molecular glue.},
keywords = {carbon, I2CT, Nanotubes, Oxidation-Reduction, Silicon, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Sidewall functionalized multi-walled carbon nanotubes can be covalently bound parallel to a silicon surface via a self-assembled acid-terminated monolayer used as an organic molecular glue.
Dumortier Hélène, Lacotte Stéphanie, Pastorin Giorgia, Marega Riccardo, Wu Wei, Bonifazi Davide, Briand Jean-Paul, Prato Maurizio, Muller Sylviane, Bianco Alberto
Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells Journal Article
In: Nano Letters, vol. 6, no. 7, pp. 1522–1528, 2006, ISSN: 1530-6984.
Abstract | Links | BibTeX | Tags: Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco
@article{dumortier_functionalized_2006b,
title = {Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells},
author = {Hélène Dumortier and Stéphanie Lacotte and Giorgia Pastorin and Riccardo Marega and Wei Wu and Davide Bonifazi and Jean-Paul Briand and Maurizio Prato and Sylviane Muller and Alberto Bianco},
doi = {10.1021/nl061160x},
issn = {1530-6984},
year = {2006},
date = {2006-07-01},
journal = {Nano Letters},
volume = {6},
number = {7},
pages = {1522--1528},
abstract = {Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.},
keywords = {Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.
Pastorin Giorgia, Marchesan Silvia, Hoebeke Johan, Ros Tatiana Da, Ehret-Sabatier Laurence, Briand Jean-Paul, Prato Maurizio, Bianco Alberto
Design and activity of cationic fullerene derivatives as inhibitors of acetylcholinesterase Journal Article
In: Organic & Biomolecular Chemistry, vol. 4, no. 13, pp. 2556–2562, 2006, ISSN: 1477-0520.
Abstract | Links | BibTeX | Tags: Acetylcholinesterase, Binding Sites, Cations, Cholinesterase Inhibitors, Drug Design, Fullerenes, I2CT, Models, Molecular, Team-Bianco
@article{pastorin_design_2006,
title = {Design and activity of cationic fullerene derivatives as inhibitors of acetylcholinesterase},
author = {Giorgia Pastorin and Silvia Marchesan and Johan Hoebeke and Tatiana Da Ros and Laurence Ehret-Sabatier and Jean-Paul Briand and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b604361e},
issn = {1477-0520},
year = {2006},
date = {2006-07-01},
journal = {Organic & Biomolecular Chemistry},
volume = {4},
number = {13},
pages = {2556--2562},
abstract = {Four different regioisomers of cationic bis-N,N-dimethylfulleropyrrolidinium salts have been prepared and evaluated as inhibitors of the enzymatic activity of acetylcholinesterase. These fullerene-based derivatives were found to be noncompetitive inhibitors of acetylthiocholine hydrolysis. Molecular modelling was used to describe the possible interactions between the fullerene cage and the amino acids surrounding the cavity of the enzyme. The cationic C(60) derivatives used in this study represent a new class of molecules potentially able to modulate the enzymatic activity of acetylcholinesterase.},
keywords = {Acetylcholinesterase, Binding Sites, Cations, Cholinesterase Inhibitors, Drug Design, Fullerenes, I2CT, Models, Molecular, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Four different regioisomers of cationic bis-N,N-dimethylfulleropyrrolidinium salts have been prepared and evaluated as inhibitors of the enzymatic activity of acetylcholinesterase. These fullerene-based derivatives were found to be noncompetitive inhibitors of acetylthiocholine hydrolysis. Molecular modelling was used to describe the possible interactions between the fullerene cage and the amino acids surrounding the cavity of the enzyme. The cationic C(60) derivatives used in this study represent a new class of molecules potentially able to modulate the enzymatic activity of acetylcholinesterase.
Bianco Alberto, Maggini Michele, Nogarole Marco, Scorrano Gianfranco
Hydrolysis Rate of Functionalized Fullerenes Bearing Alkoxysilanes: A Comparative Study Journal Article
In: European Journal of Organic Chemistry, vol. 2006, no. 13, pp. 2934–2941, 2006, ISSN: 1434-193X.
Abstract | Links | BibTeX | Tags: Alkoxysilanes, Fullerenes, Fulleropyrrolidines, I2CT, Sol–gel, Team-Bianco
@article{bianco_hydrolysis_2006,
title = {Hydrolysis Rate of Functionalized Fullerenes Bearing Alkoxysilanes: A Comparative Study},
author = {Alberto Bianco and Michele Maggini and Marco Nogarole and Gianfranco Scorrano},
url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/ejoc.200600084},
doi = {10.1002/ejoc.200600084},
issn = {1434-193X},
year = {2006},
date = {2006-07-01},
urldate = {2020-03-31},
journal = {European Journal of Organic Chemistry},
volume = {2006},
number = {13},
pages = {2934--2941},
abstract = {Abstract Soluble fulleropyrrolidines bearing a trialkoxysilyl functionality (methoxy, ethoxy, butoxy, and isopropoxy) have been prepared and characterized. The hydrolysis rate constant for each fulleropyrrolidine was measured with 1H NMR spectroscopy by following the disappearance of selected resonances of the fullerene substrate under the conditions (HCl/H2O/THF) used for the preparation of fullerene-doped sol?gel glasses. It has been found that fulleropyrrolidine 1, bearing the trimethoxysilyl group, hydrolyzes faster than substrates 2?7 and should be the reagent of choice to minimize aggregation of the fullerene spheroid in sol?gel glassy matrices. The triethoxysilyl derivative 2, our benchmark fulleropyrrolidine for incorporation in sol?gel glasses, has the secondfastest hydrolysis rate. (? Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)},
keywords = {Alkoxysilanes, Fullerenes, Fulleropyrrolidines, I2CT, Sol–gel, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Abstract Soluble fulleropyrrolidines bearing a trialkoxysilyl functionality (methoxy, ethoxy, butoxy, and isopropoxy) have been prepared and characterized. The hydrolysis rate constant for each fulleropyrrolidine was measured with 1H NMR spectroscopy by following the disappearance of selected resonances of the fullerene substrate under the conditions (HCl/H2O/THF) used for the preparation of fullerene-doped sol?gel glasses. It has been found that fulleropyrrolidine 1, bearing the trimethoxysilyl group, hydrolyzes faster than substrates 2?7 and should be the reagent of choice to minimize aggregation of the fullerene spheroid in sol?gel glassy matrices. The triethoxysilyl derivative 2, our benchmark fulleropyrrolidine for incorporation in sol?gel glasses, has the secondfastest hydrolysis rate. (? Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Dumortier Hélène, Lacotte Stéphanie, Pastorin Giorgia, Marega Riccardo, Wu Wei, Bonifazi Davide, Briand Jean-Paul, Prato Maurizio, Muller Sylviane, Bianco Alberto
Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells Journal Article
In: Nano Letters, vol. 6, no. 7, pp. 1522–1528, 2006, ISSN: 1530-6984.
Abstract | Links | BibTeX | Tags: Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, Dumortier, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco, Team-Dumortier
@article{dumortier_functionalized_2006,
title = {Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells},
author = {Hélène Dumortier and Stéphanie Lacotte and Giorgia Pastorin and Riccardo Marega and Wei Wu and Davide Bonifazi and Jean-Paul Briand and Maurizio Prato and Sylviane Muller and Alberto Bianco},
doi = {10.1021/nl061160x},
issn = {1530-6984},
year = {2006},
date = {2006-07-01},
journal = {Nano Letters},
volume = {6},
number = {7},
pages = {1522--1528},
abstract = {Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.},
keywords = {Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, Dumortier, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.
Bianco Alberto, Fournel Sylvie, Wieckowski Sébastien, Hoebeke Johan, Guichard Gilles
Solid-phase synthesis of CD40L mimetics Journal Article
In: Organic & Biomolecular Chemistry, vol. 4, no. 8, pp. 1461–1463, 2006, ISSN: 1477-0520.
Abstract | Links | BibTeX | Tags: Apoptosis, CD40 Ligand, Cell Line, Chromatography, Combinatorial Chemistry Techniques, High Pressure Liquid, Humans, I2CT, Molecular Mimicry, Molecular Structure, Protein Binding, Team-Bianco, tumor
@article{bianco_solid-phase_2006,
title = {Solid-phase synthesis of CD40L mimetics},
author = {Alberto Bianco and Sylvie Fournel and Sébastien Wieckowski and Johan Hoebeke and Gilles Guichard},
doi = {10.1039/b601528j},
issn = {1477-0520},
year = {2006},
date = {2006-04-01},
journal = {Organic & Biomolecular Chemistry},
volume = {4},
number = {8},
pages = {1461--1463},
abstract = {The C3-symmetric molecule has been previously shown to mimic CD40 ligand (CD40L) homotrimers and to display effector functions. This molecule consists of a cyclic hexapeptide core containing the repetition of the D-Ala-L-Lys motif. The side chains of the lysine residues have been modified by appending the CD40L-derived sequence 143Lys-Gly-Tyr-Tyr146 via a 6-aminohexanoic acid residue as a spacer. The present report describes a general solid-phase synthesis approach to and related trimeric architectures. In addition, their CD40 binding properties as well as their effector functions have been evaluated.},
keywords = {Apoptosis, CD40 Ligand, Cell Line, Chromatography, Combinatorial Chemistry Techniques, High Pressure Liquid, Humans, I2CT, Molecular Mimicry, Molecular Structure, Protein Binding, Team-Bianco, tumor},
pubstate = {published},
tppubtype = {article}
}
The C3-symmetric molecule has been previously shown to mimic CD40 ligand (CD40L) homotrimers and to display effector functions. This molecule consists of a cyclic hexapeptide core containing the repetition of the D-Ala-L-Lys motif. The side chains of the lysine residues have been modified by appending the CD40L-derived sequence 143Lys-Gly-Tyr-Tyr146 via a 6-aminohexanoic acid residue as a spacer. The present report describes a general solid-phase synthesis approach to and related trimeric architectures. In addition, their CD40 binding properties as well as their effector functions have been evaluated.
Tasis Dimitrios, Tagmatarchis Nikos, Bianco Alberto, Prato Maurizio
Chemistry of carbon nanotubes Journal Article
In: Chemical Reviews, vol. 106, no. 3, pp. 1105–1136, 2006, ISSN: 0009-2665.
Links | BibTeX | Tags: Animals, Biopolymers, carbon, Chemical Phenomena, Chemistry, I2CT, Molecular Structure, Nanotubes, Solubility, Surface Properties, Team-Bianco
@article{tasis_chemistry_2006,
title = {Chemistry of carbon nanotubes},
author = {Dimitrios Tasis and Nikos Tagmatarchis and Alberto Bianco and Maurizio Prato},
doi = {10.1021/cr050569o},
issn = {0009-2665},
year = {2006},
date = {2006-03-01},
journal = {Chemical Reviews},
volume = {106},
number = {3},
pages = {1105--1136},
keywords = {Animals, Biopolymers, carbon, Chemical Phenomena, Chemistry, I2CT, Molecular Structure, Nanotubes, Solubility, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Pastorin Giorgia, Wu Wei, Wieckowski Sébastien, Briand Jean-Paul, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Double functionalization of carbon nanotubes for multimodal drug delivery Journal Article
In: Chemical Communications (Cambridge, England), no. 11, pp. 1182–1184, 2006, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: Azo Compounds, carbon, Cyclization, Drug Carriers, I2CT, Microscopy, Nanotubes, Pharmaceutical Preparations, Scanning Tunneling, Solubility, Team-Bianco
@article{pastorin_double_2006,
title = {Double functionalization of carbon nanotubes for multimodal drug delivery},
author = {Giorgia Pastorin and Wei Wu and Sébastien Wieckowski and Jean-Paul Briand and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b516309a},
issn = {1359-7345},
year = {2006},
date = {2006-03-01},
journal = {Chemical Communications (Cambridge, England)},
number = {11},
pages = {1182--1184},
abstract = {Multi-walled carbon nanotubes have been covalently functionalized via 1,3-dipolar cycloaddition of azomethine ylides with orthogonally protected amino functions that can be selectively deprotected and subsequently modified with drugs and fluorescent probes.},
keywords = {Azo Compounds, carbon, Cyclization, Drug Carriers, I2CT, Microscopy, Nanotubes, Pharmaceutical Preparations, Scanning Tunneling, Solubility, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Multi-walled carbon nanotubes have been covalently functionalized via 1,3-dipolar cycloaddition of azomethine ylides with orthogonally protected amino functions that can be selectively deprotected and subsequently modified with drugs and fluorescent probes.
Brough Peter, Klumpp Cedric, Bianco Alberto, Campidelli Stephane, Prato Maurizio
[60]fullerene-pyrrolidine-N-oxides Journal Article
In: The Journal of Organic Chemistry, vol. 71, no. 5, pp. 2014–2020, 2006, ISSN: 0022-3263.
Abstract | Links | BibTeX | Tags: Chromatography, Fullerenes, High Pressure Liquid, I2CT, Nitrogen, Oxidation-Reduction, Oxides, Pyrrolidines, Spectrum Analysis, Team-Bianco
@article{brough_60fullerene-pyrrolidine-n-oxides_2006,
title = {[60]fullerene-pyrrolidine-N-oxides},
author = {Peter Brough and Cedric Klumpp and Alberto Bianco and Stephane Campidelli and Maurizio Prato},
doi = {10.1021/jo052388s},
issn = {0022-3263},
year = {2006},
date = {2006-03-01},
journal = {The Journal of Organic Chemistry},
volume = {71},
number = {5},
pages = {2014--2020},
abstract = {Eight members of a new family of fullerene derivatives, [60]fulleropyrrolidine-N-oxides, have been synthesized and characterized. Facile oxidation, by a peracid, of the parent [60]fulleropyrrolidine gave clean conversions into the product molecules, in which the tertiary amine is transformed into a quaternary amine bearing an oxygen atom. The reaction is very selective, favoring the nitrogen atom of the pyrrolidine ring in preference to epoxidation of the fullerene cage. The 1H NMR shows an AB quartet splitting pattern, characteristic of nonequivalent hydrogens in the pyrrolidine ring and at a chemical shift displacement of 0.8 ppm downfield. Other methods of characterization are described, including MS, differential scanning calorimetry, thermogravimetric analysis, HPLC, UV/vis, and IR. Conclusive evidence for the formation of an N-oxide moiety is provided by the synthesis, oxidation, and NMR characterization of a novel [60]fulleropyrrolidine containing a 15N isotope, showing an 85 ppm downfield heteroatom chemical shift. Preliminary details of the effects of substitution on the reactivity of the pyrrolidine ring are also reported.},
keywords = {Chromatography, Fullerenes, High Pressure Liquid, I2CT, Nitrogen, Oxidation-Reduction, Oxides, Pyrrolidines, Spectrum Analysis, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Eight members of a new family of fullerene derivatives, [60]fulleropyrrolidine-N-oxides, have been synthesized and characterized. Facile oxidation, by a peracid, of the parent [60]fulleropyrrolidine gave clean conversions into the product molecules, in which the tertiary amine is transformed into a quaternary amine bearing an oxygen atom. The reaction is very selective, favoring the nitrogen atom of the pyrrolidine ring in preference to epoxidation of the fullerene cage. The 1H NMR shows an AB quartet splitting pattern, characteristic of nonequivalent hydrogens in the pyrrolidine ring and at a chemical shift displacement of 0.8 ppm downfield. Other methods of characterization are described, including MS, differential scanning calorimetry, thermogravimetric analysis, HPLC, UV/vis, and IR. Conclusive evidence for the formation of an N-oxide moiety is provided by the synthesis, oxidation, and NMR characterization of a novel [60]fulleropyrrolidine containing a 15N isotope, showing an 85 ppm downfield heteroatom chemical shift. Preliminary details of the effects of substitution on the reactivity of the pyrrolidine ring are also reported.
Klumpp Cédric, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Functionalized carbon nanotubes as emerging nanovectors for the delivery of therapeutics Journal Article
In: Biochimica Et Biophysica Acta, vol. 1758, no. 3, pp. 404–412, 2006, ISSN: 0006-3002.
Abstract | Links | BibTeX | Tags: carbon, DNA, Drug Carriers, Drug Delivery Systems, Humans, I2CT, Nanotubes, RNA, Team-Bianco
@article{klumpp_functionalized_2006,
title = {Functionalized carbon nanotubes as emerging nanovectors for the delivery of therapeutics},
author = {Cédric Klumpp and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1016/j.bbamem.2005.10.008},
issn = {0006-3002},
year = {2006},
date = {2006-03-01},
journal = {Biochimica Et Biophysica Acta},
volume = {1758},
number = {3},
pages = {404--412},
abstract = {Functionalized carbon nanotubes (f-CNT) are emerging as a new family of nanovectors for the delivery of different types of therapeutic molecules. The application of CNT in the field of carrier-mediated delivery has become possible after the recent discovery of their capacity to penetrate into the cells. CNT can be loaded with active molecules by forming stable covalent bonds or supramolecular assemblies based on noncovalent interactions. Once the cargos are carried into various cells, tissues and organs they are able to express their biological function. In this review, we will describe the potential of f-CNT to deliver different types of therapeutic molecules.},
keywords = {carbon, DNA, Drug Carriers, Drug Delivery Systems, Humans, I2CT, Nanotubes, RNA, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalized carbon nanotubes (f-CNT) are emerging as a new family of nanovectors for the delivery of different types of therapeutic molecules. The application of CNT in the field of carrier-mediated delivery has become possible after the recent discovery of their capacity to penetrate into the cells. CNT can be loaded with active molecules by forming stable covalent bonds or supramolecular assemblies based on noncovalent interactions. Once the cargos are carried into various cells, tissues and organs they are able to express their biological function. In this review, we will describe the potential of f-CNT to deliver different types of therapeutic molecules.
Singh Ravi, Pantarotto Davide, Lacerda Lara, Pastorin Giorgia, Klumpp Cédric, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers Journal Article
In: Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 9, pp. 3357–3362, 2006, ISSN: 0027-8424.
Abstract | Links | BibTeX | Tags: Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission
@article{singh_tissue_2006,
title = {Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers},
author = {Ravi Singh and Davide Pantarotto and Lara Lacerda and Giorgia Pastorin and Cédric Klumpp and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1073/pnas.0509009103},
issn = {0027-8424},
year = {2006},
date = {2006-02-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {103},
number = {9},
pages = {3357--3362},
abstract = {Carbon nanotubes (CNT) are intensively being developed for biomedical applications including drug and gene delivery. Although all possible clinical applications will require compatibility of CNT with the biological milieu, their in vivo capabilities and limitations have not yet been explored. In this work, water-soluble, single-walled CNT (SWNT) have been functionalized with the chelating molecule diethylentriaminepentaacetic (DTPA) and labeled with indium ((111)In) for imaging purposes. Intravenous (i.v.) administration of these functionalized SWNT (f-SWNT) followed by radioactivity tracing using gamma scintigraphy indicated that f-SWNT are not retained in any of the reticuloendothelial system organs (liver or spleen) and are rapidly cleared from systemic blood circulation through the renal excretion route. The observed rapid blood clearance and half-life (3 h) of f-SWNT has major implications for all potential clinical uses of CNT. Moreover, urine excretion studies using both f-SWNT and functionalized multiwalled CNT followed by electron microscopy analysis of urine samples revealed that both types of nanotubes were excreted as intact nanotubes. This work describes the pharmacokinetic parameters of i.v. administered functionalized CNT relevant for various therapeutic and diagnostic applications.},
keywords = {Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNT) are intensively being developed for biomedical applications including drug and gene delivery. Although all possible clinical applications will require compatibility of CNT with the biological milieu, their in vivo capabilities and limitations have not yet been explored. In this work, water-soluble, single-walled CNT (SWNT) have been functionalized with the chelating molecule diethylentriaminepentaacetic (DTPA) and labeled with indium ((111)In) for imaging purposes. Intravenous (i.v.) administration of these functionalized SWNT (f-SWNT) followed by radioactivity tracing using gamma scintigraphy indicated that f-SWNT are not retained in any of the reticuloendothelial system organs (liver or spleen) and are rapidly cleared from systemic blood circulation through the renal excretion route. The observed rapid blood clearance and half-life (3 h) of f-SWNT has major implications for all potential clinical uses of CNT. Moreover, urine excretion studies using both f-SWNT and functionalized multiwalled CNT followed by electron microscopy analysis of urine samples revealed that both types of nanotubes were excreted as intact nanotubes. This work describes the pharmacokinetic parameters of i.v. administered functionalized CNT relevant for various therapeutic and diagnostic applications.
Campidelli Stéphane, Klumpp Cédric, Bianco Alberto, Guldi Dirk M, Prato Maurizio
Functionalization of CNT: synthesis and applications in photovoltaics and biology Journal Article
In: Journal of Physical Organic Chemistry, vol. 19, no. 8-9, pp. 531–539, 2006, ISSN: 1099-1395.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, Cells, Drug delivery, electron transfer, Functionalization, I2CT, Peptides, photovoltaic, Team-Bianco, Toxicity, Vectors
@article{campidelli_functionalization_2006,
title = {Functionalization of CNT: synthesis and applications in photovoltaics and biology},
author = {Stéphane Campidelli and Cédric Klumpp and Alberto Bianco and Dirk M Guldi and Maurizio Prato},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/poc.1052},
doi = {10.1002/poc.1052},
issn = {1099-1395},
year = {2006},
date = {2006-01-01},
urldate = {2020-03-31},
journal = {Journal of Physical Organic Chemistry},
volume = {19},
number = {8-9},
pages = {531--539},
abstract = {Here, we review part of the work carried out in our laboratories on carbon nanotube functionalization. Both covalent (sidewall derivatization) and non-covalent (using π-π interactions) functionalization have been used to solubilize carbon nanotubes (NTs). The combination of NTs with various electron donors, mainly using the supramolecular approach, led to a new generation of donor-acceptor nanohybrids which can be used for the development of carbon-based photovoltaic cells. Covalent functionalization has been successfully applied for preparation of water soluble nanotubes and further derivatization of the nanotubes with bioactive molecules hold great promise for application in drug, vaccine and gene delivery. Copyright © 2006 John Wiley & Sons, Ltd.},
keywords = {Carbon nanotubes, Cells, Drug delivery, electron transfer, Functionalization, I2CT, Peptides, photovoltaic, Team-Bianco, Toxicity, Vectors},
pubstate = {published},
tppubtype = {article}
}
Here, we review part of the work carried out in our laboratories on carbon nanotube functionalization. Both covalent (sidewall derivatization) and non-covalent (using π-π interactions) functionalization have been used to solubilize carbon nanotubes (NTs). The combination of NTs with various electron donors, mainly using the supramolecular approach, led to a new generation of donor-acceptor nanohybrids which can be used for the development of carbon-based photovoltaic cells. Covalent functionalization has been successfully applied for preparation of water soluble nanotubes and further derivatization of the nanotubes with bioactive molecules hold great promise for application in drug, vaccine and gene delivery. Copyright © 2006 John Wiley & Sons, Ltd.
Lacerda L, Pastorin G, Wu W, Prato M, Bianco A, Kostarelos K
In: Advanced Functional Materials, vol. 16, no. 14, pp. 1839–1846, 2006, ISSN: 1616-3028.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, DNA, I2CT, Luminescence, multiwalled, single-walled, Team-Bianco
@article{lacerda_luminescence_2006,
title = {Luminescence of Functionalized Carbon Nanotubes as a Tool to Monitor Bundle Formation and Dissociation in Water: The Effect of Plasmid-DNA Complexation},
author = {L Lacerda and G Pastorin and W Wu and M Prato and A Bianco and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.200500569},
doi = {10.1002/adfm.200500569},
issn = {1616-3028},
year = {2006},
date = {2006-01-01},
urldate = {2020-03-31},
journal = {Advanced Functional Materials},
volume = {16},
number = {14},
pages = {1839--1846},
abstract = {Functionalized carbon nanotubes (f-CNTs) are explored as novel nanomaterials for biomedical applications. UV-vis luminescence of aqueous dispersions of CNT–NH3+ and CNT–NH–Ac (NH–Ac: acetamido) is observed using standard laboratory spectrophotometric instrumentation, and the measured fluorescence intensity is correlated with the aggregation state of the f-CNTs: a high intensity indicates improved f-CNT individualization and dispersion, while a decrease in fluorescence intensity indicates a higher degree of nanotube aggregation and bundling as a result of varying the sodium dodecyl sulfate (SDS) concentrations and pH in the aqueous phase. Moreover, utilization of this relationship between fluorescence intensity and the state of f-CNT aggregation is carried out to elucidate the interactions between f-CNTs and gene-encoding plasmid DNA (pDNA). pDNA is shown to interact with CNT–NH3+ primarily through electrostatic interactions that lead concomitantly to a higher degree of f-CNT bundling. The CNT–NH3+/pDNA interactions are successfully competed by SDS/f-CNT surface interactions, resulting in the displacement of pDNA. These studies provide exemplification of the use of fluorescence spectrophotometry to accurately describe the aggregation state of water-soluble f-CNTs. Characterization of the complexes between pDNA and f-CNTs elucidates the opportunities and limitations of such supramolecular systems as potential vectors for gene transfer.},
keywords = {Carbon nanotubes, DNA, I2CT, Luminescence, multiwalled, single-walled, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalized carbon nanotubes (f-CNTs) are explored as novel nanomaterials for biomedical applications. UV-vis luminescence of aqueous dispersions of CNT–NH3+ and CNT–NH–Ac (NH–Ac: acetamido) is observed using standard laboratory spectrophotometric instrumentation, and the measured fluorescence intensity is correlated with the aggregation state of the f-CNTs: a high intensity indicates improved f-CNT individualization and dispersion, while a decrease in fluorescence intensity indicates a higher degree of nanotube aggregation and bundling as a result of varying the sodium dodecyl sulfate (SDS) concentrations and pH in the aqueous phase. Moreover, utilization of this relationship between fluorescence intensity and the state of f-CNT aggregation is carried out to elucidate the interactions between f-CNTs and gene-encoding plasmid DNA (pDNA). pDNA is shown to interact with CNT–NH3+ primarily through electrostatic interactions that lead concomitantly to a higher degree of f-CNT bundling. The CNT–NH3+/pDNA interactions are successfully competed by SDS/f-CNT surface interactions, resulting in the displacement of pDNA. These studies provide exemplification of the use of fluorescence spectrophotometry to accurately describe the aggregation state of water-soluble f-CNTs. Characterization of the complexes between pDNA and f-CNTs elucidates the opportunities and limitations of such supramolecular systems as potential vectors for gene transfer.
2005
Fournel Sylvie, Wieckowski Sébastien, Sun Weimin, Trouche Nathalie, Dumortier Hélène, Bianco Alberto, Chaloin Olivier, Habib Mohammed, Peter Jean-Christophe, Schneider Pascal, Vray Bernard, Toes René E, Offringa Rienk, Melief Cornelis J M, Hoebeke Johan, Guichard Gilles
C3-symmetric peptide scaffolds are functional mimetics of trimeric CD40L Journal Article
In: Nature Chemical Biology, vol. 1, no. 7, pp. 377–382, 2005, ISSN: 1552-4450.
Abstract | Links | BibTeX | Tags: Animals, Apoptosis, Biological, CD40 Antigens, CD40 Ligand, Cell Line, Dumortier, Humans, I2CT, Inbred BALB C, Mice, Models, Molecular Mimicry, Molecular Structure, Peptides, Protein Conformation, Protein Structure, Quaternary, Structure-Activity Relationship, Team-Bianco, Team-Dumortier, Time Factors, tumor
@article{fournel_c3-symmetric_2005,
title = {C3-symmetric peptide scaffolds are functional mimetics of trimeric CD40L},
author = {Sylvie Fournel and Sébastien Wieckowski and Weimin Sun and Nathalie Trouche and Hélène Dumortier and Alberto Bianco and Olivier Chaloin and Mohammed Habib and Jean-Christophe Peter and Pascal Schneider and Bernard Vray and René E Toes and Rienk Offringa and Cornelis J M Melief and Johan Hoebeke and Gilles Guichard},
doi = {10.1038/nchembio746},
issn = {1552-4450},
year = {2005},
date = {2005-12-01},
journal = {Nature Chemical Biology},
volume = {1},
number = {7},
pages = {377--382},
abstract = {Interaction between CD40, a member of the tumor necrosis factor receptor (TNFR) superfamily, and its ligand CD40L, a 39-kDa glycoprotein, is essential for the development of humoral and cellular immune responses. Selective blockade or activation of this pathway provides the ground for the development of new treatments against immunologically based diseases and malignancies. Like other members of the TNF superfamily, CD40L monomers self-assemble around a threefold symmetry axis to form noncovalent homotrimers that can each bind three receptor molecules. Here, we report on the structure-based design of small synthetic molecules with C3 symmetry that can mimic CD40L homotrimers. These molecules interact with CD40, compete with the binding of CD40L to CD40, and reproduce, to a certain extent, the functional properties of the much larger homotrimeric soluble CD40L. Architectures based on rigid C3-symmetric cores may thus represent a general approach to mimicking homotrimers of the TNF superfamily.},
keywords = {Animals, Apoptosis, Biological, CD40 Antigens, CD40 Ligand, Cell Line, Dumortier, Humans, I2CT, Inbred BALB C, Mice, Models, Molecular Mimicry, Molecular Structure, Peptides, Protein Conformation, Protein Structure, Quaternary, Structure-Activity Relationship, Team-Bianco, Team-Dumortier, Time Factors, tumor},
pubstate = {published},
tppubtype = {article}
}
Interaction between CD40, a member of the tumor necrosis factor receptor (TNFR) superfamily, and its ligand CD40L, a 39-kDa glycoprotein, is essential for the development of humoral and cellular immune responses. Selective blockade or activation of this pathway provides the ground for the development of new treatments against immunologically based diseases and malignancies. Like other members of the TNF superfamily, CD40L monomers self-assemble around a threefold symmetry axis to form noncovalent homotrimers that can each bind three receptor molecules. Here, we report on the structure-based design of small synthetic molecules with C3 symmetry that can mimic CD40L homotrimers. These molecules interact with CD40, compete with the binding of CD40L to CD40, and reproduce, to a certain extent, the functional properties of the much larger homotrimeric soluble CD40L. Architectures based on rigid C3-symmetric cores may thus represent a general approach to mimicking homotrimers of the TNF superfamily.
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Applications of carbon nanotubes in drug delivery Journal Article
In: Current Opinion in Chemical Biology, vol. 9, no. 6, pp. 674–679, 2005, ISSN: 1367-5931.
Abstract | Links | BibTeX | Tags: Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco
@article{bianco_applications_2005,
title = {Applications of carbon nanotubes in drug delivery},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1016/j.cbpa.2005.10.005},
issn = {1367-5931},
year = {2005},
date = {2005-12-01},
journal = {Current Opinion in Chemical Biology},
volume = {9},
number = {6},
pages = {674--679},
abstract = {The development of new and efficient drug delivery systems is of fundamental importance to improve the pharmacological profiles of many classes of therapeutic molecules. Many different types of drug delivery systems are currently available. Within the family of nanomaterials, carbon nanotubes (CNT) have emerged as a new alternative and efficient tool for transporting and translocating therapeutic molecules. CNT can be functionalised with bioactive peptides, proteins, nucleic acids and drugs, and used to deliver their cargos to cells and organs. Because functionalised CNT display low toxicity and are not immunogenic, such systems hold great potential in the field of nanobiotechnology and nanomedicine.},
keywords = {Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The development of new and efficient drug delivery systems is of fundamental importance to improve the pharmacological profiles of many classes of therapeutic molecules. Many different types of drug delivery systems are currently available. Within the family of nanomaterials, carbon nanotubes (CNT) have emerged as a new alternative and efficient tool for transporting and translocating therapeutic molecules. CNT can be functionalised with bioactive peptides, proteins, nucleic acids and drugs, and used to deliver their cargos to cells and organs. Because functionalised CNT display low toxicity and are not immunogenic, such systems hold great potential in the field of nanobiotechnology and nanomedicine.
Wu Wei, Wieckowski Sébastien, Pastorin Giorgia, Benincasa Monica, Klumpp Cédric, Briand Jean-Paul, Gennaro Renato, Prato Maurizio, Bianco Alberto
Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes Journal Article
In: Angewandte Chemie (International Ed. in English), vol. 44, no. 39, pp. 6358–6362, 2005, ISSN: 1433-7851.
Links | BibTeX | Tags: Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco
@article{wu_targeted_2005,
title = {Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes},
author = {Wei Wu and Sébastien Wieckowski and Giorgia Pastorin and Monica Benincasa and Cédric Klumpp and Jean-Paul Briand and Renato Gennaro and Maurizio Prato and Alberto Bianco},
doi = {10.1002/anie.200501613},
issn = {1433-7851},
year = {2005},
date = {2005-10-01},
journal = {Angewandte Chemie (International Ed. in English)},
volume = {44},
number = {39},
pages = {6358--6362},
keywords = {Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto
Efficient solid-phase synthesis of fullero-peptides using Merrifield strategy Journal Article
In: Chemical Communications (Cambridge, England), no. 25, pp. 3174–3176, 2005, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: Chromatography, Fullerenes, High Pressure Liquid, I2CT, Mass Spectrometry, Peptides, Team-Bianco
@article{bianco_efficient_2005,
title = {Efficient solid-phase synthesis of fullero-peptides using Merrifield strategy},
author = {Alberto Bianco},
doi = {10.1039/b504659a},
issn = {1359-7345},
year = {2005},
date = {2005-07-01},
journal = {Chemical Communications (Cambridge, England)},
number = {25},
pages = {3174--3176},
abstract = {Boc-protected l-fulleropyrrolidino-glutamic acid was readily prepared and employed for the synthesis of fullerene-containing peptides using the solid-phase Boc chemistry developed by Merrifield.},
keywords = {Chromatography, Fullerenes, High Pressure Liquid, I2CT, Mass Spectrometry, Peptides, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Boc-protected l-fulleropyrrolidino-glutamic acid was readily prepared and employed for the synthesis of fullerene-containing peptides using the solid-phase Boc chemistry developed by Merrifield.
Bianco Alberto, Hoebeke Johan, Kostarelos Kostas, Prato Maurizio, Partidos Charalambos D
Carbon nanotubes: on the road to deliver Journal Article
In: Current Drug Delivery, vol. 2, no. 3, pp. 253–259, 2005, ISSN: 1567-2018.
Abstract | Links | BibTeX | Tags: carbon, CpG Islands, DNA, Drug Carriers, I2CT, nanotechnology, Team-Bianco
@article{bianco_carbon_2005,
title = {Carbon nanotubes: on the road to deliver},
author = {Alberto Bianco and Johan Hoebeke and Kostas Kostarelos and Maurizio Prato and Charalambos D Partidos},
doi = {10.2174/1567201054367959},
issn = {1567-2018},
year = {2005},
date = {2005-07-01},
journal = {Current Drug Delivery},
volume = {2},
number = {3},
pages = {253--259},
abstract = {Over the last few years, considerable advances have been made in the field of nanotechnology. The advent of carbon nanotube functionalization has paved the way for their potential application as a delivery system of diverse molecules such as peptides, proteins, plasmid DNA, and synthetic oligodeoxynucleotides. This opens new therapeutic and preventive opportunities to combat diseases. The scope of this review is to summarize our recent work in this rapidly growing field.},
keywords = {carbon, CpG Islands, DNA, Drug Carriers, I2CT, nanotechnology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Over the last few years, considerable advances have been made in the field of nanotechnology. The advent of carbon nanotube functionalization has paved the way for their potential application as a delivery system of diverse molecules such as peptides, proteins, plasmid DNA, and synthetic oligodeoxynucleotides. This opens new therapeutic and preventive opportunities to combat diseases. The scope of this review is to summarize our recent work in this rapidly growing field.
Pastorin Giorgia, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Functionalized Carbon Nanotubes: Towards the Delivery of Therapeutic Molecules Journal Article
In: Journal of Biomedical Nanotechnology, vol. 1, no. 2, pp. 133–142, 2005, ISSN: 15507033, 00000000.
Links | BibTeX | Tags: I2CT, Team-Bianco
@article{pastorin_functionalized_2005,
title = {Functionalized Carbon Nanotubes: Towards the Delivery of Therapeutic Molecules},
author = {Giorgia Pastorin and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
url = {http://www.ingentaselect.com/rpsv/cgi-bin/cgi?ini=xref&body=linker&reqdoi=10.1166/jbn.2005.017},
doi = {10.1166/jbn.2005.017},
issn = {15507033, 00000000},
year = {2005},
date = {2005-06-01},
urldate = {2020-11-26},
journal = {Journal of Biomedical Nanotechnology},
volume = {1},
number = {2},
pages = {133--142},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Singh Ravi, Pantarotto Davide, McCarthy David, Chaloin Olivier, Hoebeke Johan, Partidos Charalambos D, Briand Jean-Paul, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors Journal Article
In: Journal of the American Chemical Society, vol. 127, no. 12, pp. 4388–4396, 2005, ISSN: 0002-7863.
Abstract | Links | BibTeX | Tags: carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco
@article{singh_binding_2005,
title = {Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors},
author = {Ravi Singh and Davide Pantarotto and David McCarthy and Olivier Chaloin and Johan Hoebeke and Charalambos D Partidos and Jean-Paul Briand and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1021/ja0441561},
issn = {0002-7863},
year = {2005},
date = {2005-03-01},
journal = {Journal of the American Chemical Society},
volume = {127},
number = {12},
pages = {4388--4396},
abstract = {Carbon nanotubes (CNTs) constitute a class of nanomaterials that possess characteristics suitable for a variety of possible applications. Their compatibility with aqueous environments has been made possible by the chemical functionalization of their surface, allowing for exploration of their interactions with biological components including mammalian cells. Functionalized CNTs (f-CNTs) are being intensively explored in advanced biotechnological applications ranging from molecular biosensors to cellular growth substrates. We have been exploring the potential of f-CNTs as delivery vehicles of biologically active molecules in view of possible biomedical applications, including vaccination and gene delivery. Recently we reported the capability of ammonium-functionalized single-walled CNTs to penetrate human and murine cells and facilitate the delivery of plasmid DNA leading to expression of marker genes. To optimize f-CNTs as gene delivery vehicles, it is essential to characterize their interactions with DNA. In the present report, we study the interactions of three types of f-CNTs, ammonium-functionalized single-walled and multiwalled carbon nanotubes (SWNT-NH3+; MWNT-NH3+), and lysine-functionalized single-walled carbon nanotubes (SWNT-Lys-NH3+), with plasmid DNA. Nanotube-DNA complexes were analyzed by scanning electron microscopy, surface plasmon resonance, PicoGreen dye exclusion, and agarose gel shift assay. The results indicate that all three types of cationic carbon nanotubes are able to condense DNA to varying degrees, indicating that both nanotube surface area and charge density are critical parameters that determine the interaction and electrostatic complex formation between f-CNTs with DNA. All three different f-CNT types in this study exhibited upregulation of marker gene expression over naked DNA using a mammalian (human) cell line. Differences in the levels of gene expression were correlated with the structural and biophysical data obtained for the f-CNT:DNA complexes to suggest that large surface area leading to very efficient DNA condensation is not necessary for effective gene transfer. However, it will require further investigation to determine whether the degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo. This study constitutes the first thorough investigation into the physicochemical interactions between cationic functionalized carbon nanotubes and DNA toward construction of carbon nanotube-based gene transfer vector systems.},
keywords = {carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) constitute a class of nanomaterials that possess characteristics suitable for a variety of possible applications. Their compatibility with aqueous environments has been made possible by the chemical functionalization of their surface, allowing for exploration of their interactions with biological components including mammalian cells. Functionalized CNTs (f-CNTs) are being intensively explored in advanced biotechnological applications ranging from molecular biosensors to cellular growth substrates. We have been exploring the potential of f-CNTs as delivery vehicles of biologically active molecules in view of possible biomedical applications, including vaccination and gene delivery. Recently we reported the capability of ammonium-functionalized single-walled CNTs to penetrate human and murine cells and facilitate the delivery of plasmid DNA leading to expression of marker genes. To optimize f-CNTs as gene delivery vehicles, it is essential to characterize their interactions with DNA. In the present report, we study the interactions of three types of f-CNTs, ammonium-functionalized single-walled and multiwalled carbon nanotubes (SWNT-NH3+; MWNT-NH3+), and lysine-functionalized single-walled carbon nanotubes (SWNT-Lys-NH3+), with plasmid DNA. Nanotube-DNA complexes were analyzed by scanning electron microscopy, surface plasmon resonance, PicoGreen dye exclusion, and agarose gel shift assay. The results indicate that all three types of cationic carbon nanotubes are able to condense DNA to varying degrees, indicating that both nanotube surface area and charge density are critical parameters that determine the interaction and electrostatic complex formation between f-CNTs with DNA. All three different f-CNT types in this study exhibited upregulation of marker gene expression over naked DNA using a mammalian (human) cell line. Differences in the levels of gene expression were correlated with the structural and biophysical data obtained for the f-CNT:DNA complexes to suggest that large surface area leading to very efficient DNA condensation is not necessary for effective gene transfer. However, it will require further investigation to determine whether the degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo. This study constitutes the first thorough investigation into the physicochemical interactions between cationic functionalized carbon nanotubes and DNA toward construction of carbon nanotube-based gene transfer vector systems.
