Prakash Pragya, Roychowdhury-Sinha Arghyashree, Goto Akira
Verloren negatively regulates the expression of IMD pathway dependent antimicrobial peptides in Drosophila Journal Article
In: Scientific Reports, vol. 11, no. 15549, 2021.
Abstract | Links | BibTeX | Tags: bacteria, Biochemistry, DNA, Fungi, Gene Expression, gene regulation, Genetics, hoffmann, Immunochemistry, Immunology, infection, inflammation, Innate immune cells, innate immunity, M3i, microbiology, Molecular Biology, pathogens, RNA, RNAi, Signal Transduction, Transcription
@article{Goto2021,
title = {Verloren negatively regulates the expression of IMD pathway dependent antimicrobial peptides in Drosophila},
author = {Pragya Prakash and Arghyashree Roychowdhury-Sinha and Akira Goto},
url = {https://www.nature.com/articles/s41598-021-94973-0},
doi = {10.1038/s41598-021-94973-0},
year = {2021},
date = {2021-07-30},
journal = {Scientific Reports},
volume = {11},
number = {15549},
abstract = {Drosophila immune deficiency (IMD) pathway is similar to the human tumor necrosis factor receptor (TNFR) signaling pathway and is preferentially activated by Gram-negative bacterial infection. Recent studies highlighted the importance of IMD pathway regulation as it is tightly controlled by numbers of negative regulators at multiple levels. Here, we report a new negative regulator of the IMD pathway, Verloren (Velo). Silencing of Velo led to constitutive expression of the IMD pathway dependent antimicrobial peptides (AMPs), and Escherichia coli stimulation further enhanced the AMP expression. Epistatic analysis indicated that Velo knock-down mediated AMP upregulation is dependent on the canonical members of the IMD pathway. The immune fluorescent study using overexpression constructs revealed that Velo resides both in the nucleus and cytoplasm, but the majority (~ 75%) is localized in the nucleus. We also observed from in vivo analysis that Velo knock-down flies exhibit significant upregulation of the AMP expression and reduced bacterial load. Survival experiments showed that Velo knock-down flies have a short lifespan and are susceptible to the infection of pathogenic Gram-negative bacteria, P. aeruginosa. Taken together, these data suggest that Velo is an additional new negative regulator of the IMD pathway, possibly acting in both the nucleus and cytoplasm.},
keywords = {bacteria, Biochemistry, DNA, Fungi, Gene Expression, gene regulation, Genetics, hoffmann, Immunochemistry, Immunology, infection, inflammation, Innate immune cells, innate immunity, M3i, microbiology, Molecular Biology, pathogens, RNA, RNAi, Signal Transduction, Transcription},
pubstate = {published},
tppubtype = {article}
}
Leite Thiago H J F, Ferreira Alvaro G A, Imler Jean-Luc, Marques João T
Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes Journal Article
In: Frontiers in immunology, vol. 12, pp. 660873, 2021.
Abstract | Links | BibTeX | Tags: Aedes, Dengue, Hemocytes, imler, innate immunity, M3i, Marques, Zika
@article{Leite2021,
title = {Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes},
author = {Thiago H J F Leite and Alvaro G A Ferreira and Jean-Luc Imler and João T Marques},
url = {https://www.frontiersin.org/articles/10.3389/fimmu.2021.660873/full},
doi = {10.3389/fimmu.2021.660873},
year = {2021},
date = {2021-05-13},
journal = {Frontiers in immunology},
volume = {12},
pages = {660873},
abstract = {Aedes aegypti mosquitoes are vectors for arboviruses of medical importance such as dengue (DENV) and Zika (ZIKV) viruses. Different innate immune pathways contribute to the control of arboviruses in the mosquito vector including RNA interference, Toll and Jak- STAT pathways. However, the role of cellular responses mediated by circulating macrophage-like cells known as hemocytes remains unclear. Here we show that hemocytes are recruited to the midgut of Ae. aegypti mosquitoes in response to DENV or ZIKV. Blockade of the phagocytic function of hemocytes using latex beads induced increased accumulation of hemocytes in the midgut and a reduction in virus infection levels in this organ. In contrast, inhibition of phagocytosis by hemocytes led to increased systemic dissemination and replication of DENV and ZIKV. Hence, our work reveals a dual role for hemocytes in Ae. aegypti mosquitoes, whereby phagocytosis is not required to control viral infection in the midgut but is essential to restrict systemic dissemination. Further understanding of the mechanism behind this duality could help the design of vector-based strategies to prevent transmission of arboviruses.},
keywords = {Aedes, Dengue, Hemocytes, imler, innate immunity, M3i, Marques, Zika},
pubstate = {published},
tppubtype = {article}
}
Chen Di, Roychowdhury-Sinha Arghyashree, Prakash Pragya, Lan Xiao, Fan Wenmin, Goto Akira, Hoffmann Jules A
A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects of Drosophila immune defenses Journal Article
In: PNAS, vol. 118, no. 12, 2021.
Abstract | Links | BibTeX | Tags: cancer, chemoreceptor, Drosophila melanogaster, goto, hoffmann, innate immunity, M3i, RasV12
@article{chen2021,
title = {A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects of Drosophila immune defenses},
author = {Di Chen and Arghyashree Roychowdhury-Sinha and Pragya Prakash and Xiao Lan and Wenmin Fan and Akira Goto and Jules A Hoffmann},
url = {https://www.pnas.org/content/118/12/e2100825118},
doi = {https://doi.org/10.1073/pnas.2100825118},
year = {2021},
date = {2021-03-23},
urldate = {2021-03-23},
journal = {PNAS},
volume = {118},
number = {12},
abstract = {Oncogenic RasV12 cells [A. Simcox et al., PLoS Genet. 4, e1000142 (2008)] injected into adult males proliferated massively after a lag period of several days, and led to the demise of the flies after 2 to 3 wk. The injection induced an early massive transcriptomic response that, unexpectedly, included more than 100 genes encoding chemoreceptors of various families. The kinetics of induction and the identities of the induced genes differed markedly from the responses generated by injections of microbes. Subsequently, hundreds of genes were up-regulated, attesting to intense catabolic activities in the flies, active tracheogenesis, and cuticulogenesis, as well as stress and inflammation-type responses. At 11 d after the injections, GFP-positive oncogenic cells isolated from the host flies exhibited a markedly different transcriptomic profile from that of the host and distinct from that at the time of their injection, including in particular up-regulated expression of genes typical for cells engaged in the classical antimicrobial response of Drosophila.},
keywords = {cancer, chemoreceptor, Drosophila melanogaster, goto, hoffmann, innate immunity, M3i, RasV12},
pubstate = {published},
tppubtype = {article}
}
Talide L, Imler JL, Meignin C
Sensing Viral Infections in Insects: A Dearth of Pathway Receptors Journal Article
In: Curr Issues Mol Biol, vol. 34, pp. 31-60, 2019, ISBN: 9781912530090.
Abstract | Links | BibTeX | Tags: imler, innate immunity, M3i, meignin, STAT, viral Infection
@article{Talide_2020,
title = {Sensing Viral Infections in Insects: A Dearth of Pathway Receptors},
author = {L Talide and JL Imler and C Meignin},
url = {https://www.caister.com/insectvirology2},
doi = {10.21775/cimb.034.031},
isbn = {9781912530090},
year = {2019},
date = {2019-06-06},
journal = {Curr Issues Mol Biol},
volume = {34},
pages = {31-60},
abstract = {Insects, the most diverse group of animals, can be infected by an extraordinary diversity of viruses. Among them, arthropod-borne viruses can be transmitted to humans, while bee and silkworm viruses cause important economic losses. Like all invertebrates, insects rely solely on innate immunity to counter viral infections. Protein-based mechanisms, involving restriction factors and evolutionarily conserved signaling pathways regulating transcription factors of the NF-kB and STAT families, participate in the control of viral infections in insects. In addition, RNA-based responses play a major role in the silencing of viral RNAs. We review here our current state of knowledge on insect antiviral defense mechanisms, which include conserved as well as adaptive, insect-specific strategies. Identification of the innate immunity receptors that sense viral infection in insects remains a major challenge for the field. },
keywords = {imler, innate immunity, M3i, meignin, STAT, viral Infection},
pubstate = {published},
tppubtype = {article}
}
Rive Corvin, Reina Giacomo, Wagle Prerana, Treossi Emanuele, Palermo Vincenzo, Bianco Alberto, Delogu Lucia Gemma, Rieckher Matthias, Schumacher Björn
Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans Journal Article
In: Small (Weinheim an Der Bergstrasse, Germany), vol. 15, no. 45, pp. e1902699, 2019, ISSN: 1613-6829.
Abstract | Links | BibTeX | Tags: amino-functionalized graphene oxide, Caenorhabditis elegans, graphene oxide, I2CT, innate immunity, Team-Bianco
@article{rive_improved_2019,
title = {Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans},
author = {Corvin Rive and Giacomo Reina and Prerana Wagle and Emanuele Treossi and Vincenzo Palermo and Alberto Bianco and Lucia Gemma Delogu and Matthias Rieckher and Björn Schumacher},
doi = {10.1002/smll.201902699},
issn = {1613-6829},
year = {2019},
date = {2019-01-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {15},
number = {45},
pages = {e1902699},
abstract = {Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. This study describes adverse effects of GO and amino-functionalized GO (GONH2 ) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Sequencing data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. This work establishes enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as a biomedical nanomaterial.},
keywords = {amino-functionalized graphene oxide, Caenorhabditis elegans, graphene oxide, I2CT, innate immunity, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Koltun Bella, Shackelford Eliza, Bonnay François, Matt Nicolas, Reichhart Jean-Marc, Orian Amir
The SUMO-targeted ubiquitin ligase, Dgrn, is essential for Drosophila innate immunity Journal Article
In: The International Journal of Developmental Biology, vol. 61, no. 3-4-5, pp. 319–327, 2017, ISSN: 0214-6282.
Links | BibTeX | Tags: Dgrn, Drosophila, innate immunity, Ligase, M3i, matt, reichhart, SUMO, target, ubiquitin
@article{koltun_sumo-targeted_2017,
title = {The SUMO-targeted ubiquitin ligase, Dgrn, is essential for Drosophila innate immunity},
author = {Bella Koltun and Eliza Shackelford and François Bonnay and Nicolas Matt and Jean-Marc Reichhart and Amir Orian},
url = {http://www.intjdevbiol.com/paper.php?doi=160250ao},
doi = {10.1387/ijdb.160250ao},
issn = {0214-6282},
year = {2017},
date = {2017-01-01},
urldate = {2017-07-12},
journal = {The International Journal of Developmental Biology},
volume = {61},
number = {3-4-5},
pages = {319--327},
keywords = {Dgrn, Drosophila, innate immunity, Ligase, M3i, matt, reichhart, SUMO, target, ubiquitin},
pubstate = {published},
tppubtype = {article}
}
Lamiable Olivier, Meignin Carine, Imler Jean-Luc
WntD and Diedel: Two immunomodulatory cytokines in Drosophila immunity Journal Article
In: Fly (Austin), vol. 10, no. 4, pp. 187–194, 2016, ISSN: 1933-6942.
Abstract | Links | BibTeX | Tags: Cytokines, Drosophila, IMD pathway, imler, innate immunity, M3i, meignin, virus
@article{lamiable_wntd_2016,
title = {WntD and Diedel: Two immunomodulatory cytokines in Drosophila immunity},
author = {Olivier Lamiable and Carine Meignin and Jean-Luc Imler},
url = {http://www.tandfonline.com/doi/abs/10.1080/19336934.2016.1202387?journalCode=kfly20},
doi = {10.1080/19336934.2016.1202387},
issn = {1933-6942},
year = {2016},
date = {2016-01-01},
journal = {Fly (Austin)},
volume = {10},
number = {4},
pages = {187--194},
abstract = {Remarkable progress has been made on the understanding of the basic mechanisms of innate immunity in flies, from sensing infection to production of effector molecules. However, how the immune response is orchestrated at the level of the organism remains poorly understood. While cytokines activating immune responses, such as Spaetzle or Unpaired-3, have been identified and characterized in Drosophila, much less is known regarding immunosuppressor cytokines. In a recent publication, we reported the identification of a novel cytokine, Diedel, which acts as systemic negative regulator of the IMD pathway. Here, we discuss the similarities between Diedel and WntD, another immunomodulatory cytokine and present evidence that the 2 molecules act independently from one another.},
keywords = {Cytokines, Drosophila, IMD pathway, imler, innate immunity, M3i, meignin, virus},
pubstate = {published},
tppubtype = {article}
}
Chamy Laure El, Matt Nicolas, Ntwasa Monde, Reichhart Jean-Marc
The multilayered innate immune defense of the gut Journal Article
In: Biomed J, vol. 38, no. 4, pp. 276–284, 2015, ISSN: 2320-2890.
Abstract | Links | BibTeX | Tags: fly, gut, innate immunity, M3i, matt, reichhart
@article{el_chamy_multilayered_2015,
title = {The multilayered innate immune defense of the gut},
author = {Laure El Chamy and Nicolas Matt and Monde Ntwasa and Jean-Marc Reichhart},
url = {http://www.biomedj.org/text.asp?2015/38/4/276/158621},
doi = {10.4103/2319-4170.158621},
issn = {2320-2890},
year = {2015},
date = {2015-08-01},
journal = {Biomed J},
volume = {38},
number = {4},
pages = {276--284},
abstract = {In the wild, the fruit fly Drosophila melanogaster thrives on rotten fruit. The digestive tract maintains a powerful gut immune barrier to regulate the ingested microbiota, including entomopathogenic bacteria. This gut immune barrier includes a chitinous peritrophic matrix that isolates the gut contents from the epithelial cells. In addition, the epithelial cells are tightly sealed by septate junctions and can mount an inducible immune response. This local response can be activated by invasive bacteria, or triggered by commensal bacteria in the gut lumen. As with chronic inflammation in mammals, constitutive activation of the gut innate immune response is detrimental to the health of flies. Accordingly, the Drosophila gut innate immune response is tightly regulated to maintain the endogenous microbiota, while preventing infections by pathogenic microorganisms.},
keywords = {fly, gut, innate immunity, M3i, matt, reichhart},
pubstate = {published},
tppubtype = {article}
}
Tartey Sarang, Matsushita Kazufumi, Vandenbon Alexis, Ori Daisuke, Imamura Tomoko, Mino Takashi, Standley Daron M, Hoffmann Jules A, Reichhart Jean-Marc, Akira Shizuo, Takeuchi Osamu
Akirin2 is critical for inducing inflammatory genes by bridging IκB-ζ and the SWI/SNF complex Journal Article
In: EMBO J., vol. 33, no. 20, pp. 2332–2348, 2014, ISSN: 1460-2075.
Abstract | Links | BibTeX | Tags: Adaptor Proteins, Animals, Cell Nucleus, Chromatin Assembly and Disassembly, chromatin remodeling, Chromosomal Proteins, cytokine, Cytokines, Female, Gene Expression Regulation, gene regulation, Genetic, hoffmann, Humans, Immunity, Innate, innate immunity, Knockout, Listeria monocytogenes, M3i, Macrophages, Male, Mice, Multiprotein Complexes, Non-Histone, Nuclear Proteins, Promoter Regions, Protein Binding, reichhart, Repressor Proteins, Sequence Deletion, Signal Transducing, Transcriptional Activation
@article{tartey_akirin2_2014,
title = {Akirin2 is critical for inducing inflammatory genes by bridging IκB-ζ and the SWI/SNF complex},
author = {Sarang Tartey and Kazufumi Matsushita and Alexis Vandenbon and Daisuke Ori and Tomoko Imamura and Takashi Mino and Daron M Standley and Jules A Hoffmann and Jean-Marc Reichhart and Shizuo Akira and Osamu Takeuchi},
doi = {10.15252/embj.201488447},
issn = {1460-2075},
year = {2014},
date = {2014-10-01},
journal = {EMBO J.},
volume = {33},
number = {20},
pages = {2332--2348},
abstract = {Transcription of inflammatory genes in innate immune cells is coordinately regulated by transcription factors, including NF-κB, and chromatin modifiers. However, it remains unclear how microbial sensing initiates chromatin remodeling. Here, we show that Akirin2, an evolutionarily conserved nuclear protein, bridges NF-κB and the chromatin remodeling SWI/SNF complex by interacting with BRG1-Associated Factor 60 (BAF60) proteins as well as IκB-ζ, which forms a complex with the NF-κB p50 subunit. These interactions are essential for Toll-like receptor-, RIG-I-, and Listeria-mediated expression of proinflammatory genes including Il6 and Il12b in macrophages. Consistently, effective clearance of Listeria infection required Akirin2. Furthermore, Akirin2 and IκB-ζ recruitment to the Il6 promoter depend upon the presence of IκB-ζ and Akirin2, respectively, for regulation of chromatin remodeling. BAF60 proteins were also essential for the induction of Il6 in response to LPS stimulation. Collectively, the IκB-ζ-Akirin2-BAF60 complex physically links the NF-κB and SWI/SNF complexes in innate immune cell activation. By recruiting SWI/SNF chromatin remodellers to IκB-ζ, transcriptional coactivator for NF-κB, the conserved nuclear protein Akirin2 stimulates pro-inflammatory gene promoters in mouse macrophages during innate immune responses to viral or bacterial infection.},
keywords = {Adaptor Proteins, Animals, Cell Nucleus, Chromatin Assembly and Disassembly, chromatin remodeling, Chromosomal Proteins, cytokine, Cytokines, Female, Gene Expression Regulation, gene regulation, Genetic, hoffmann, Humans, Immunity, Innate, innate immunity, Knockout, Listeria monocytogenes, M3i, Macrophages, Male, Mice, Multiprotein Complexes, Non-Histone, Nuclear Proteins, Promoter Regions, Protein Binding, reichhart, Repressor Proteins, Sequence Deletion, Signal Transducing, Transcriptional Activation},
pubstate = {published},
tppubtype = {article}
}
Imler Jean-Luc, Hoffmann Jules A
Le défi des maladies infectieuses Book Chapter
In: collection Guérir et prévenir demain, Ph. Cramer (Ed.): Chapter « L’immunité innée », pp. 167-174, Editions DOCIS, 2014, ISBN: 978-2-85525-390-9.
Abstract | BibTeX | Tags: hoffmann, imler, innate immunity, M3i, maladies infectieuses
@inbook{Imler2014,
title = {Le défi des maladies infectieuses},
author = {Jean-Luc Imler and Jules A Hoffmann},
editor = {Ph. Cramer collection Guérir et prévenir demain},
isbn = {978-2-85525-390-9},
year = {2014},
date = {2014-06-01},
pages = {167-174},
publisher = {Editions DOCIS},
chapter = {« L’immunité innée »},
abstract = {La lèpre fait des ravages dès l’Antiquité, les épidémies de peste tuent au Moyen Âge et celles de choléra dévastent l’Inde. La tuberculose émerge véritablement au XIXème siècle, la grippe espagnole a fait vingt millions de morts en 1918 et de nouvelles maladies apparaissent : les infections hospitalières, les hépatites, le SIDA, les fièvres hémorragiques, la légionellose,…
Toutes ces maladies ont un point commun, ce sont des maladies infectieuses qui sont pour la médecine un vrai défi, tant elles sont dévastatrices. L’innovation a eu et continue à avoir un rôle essentiel dans la caractérisation de ces maladies, la découverte de l’agent responsable, leur traitement et leur prévention. Ce livre, dont les auteurs font partie des plus éminents spécialistes français et européens des maladies infectieuses décrit, de façon abordable par tous, aussi bien les découvertes et les inventions essentielles à ce domaine que les avancées médicales.},
keywords = {hoffmann, imler, innate immunity, M3i, maladies infectieuses},
pubstate = {published},
tppubtype = {inbook}
}
Imler Jean-Luc
Overview of Drosophila immunity: a historical perspective Journal Article
In: Developmental and Comparative Immunology, vol. 42, no. 1, pp. 3–15, 2014, ISSN: 1879-0089.
Abstract | Links | BibTeX | Tags: Allergy and Immunology, Animal, Animals, Antimicrobial Cationic Peptides, Antimicrobial peptides, history, Humans, IMD pathway, imler, Immunity, Innate, innate immunity, M3i, Models, Pattern recognition receptors, Signal Transduction, Toll-Like Receptors
@article{imler_overview_2014,
title = {Overview of Drosophila immunity: a historical perspective},
author = {Jean-Luc Imler},
doi = {10.1016/j.dci.2013.08.018},
issn = {1879-0089},
year = {2014},
date = {2014-01-01},
journal = {Developmental and Comparative Immunology},
volume = {42},
number = {1},
pages = {3--15},
abstract = {The functional analysis of genes from the model organism Drosophila melanogaster has provided invaluable information for many cellular and developmental or physiological processes, including immunity. The best-understood aspect of Drosophila immunity is the inducible humoral response, first recognized in 1972. This pioneering work led to a remarkable series of findings over the next 30 years, ranging from the identification and characterization of the antimicrobial peptides produced, to the deciphering of the signalling pathways activating the genes that encode them and, ultimately, to the discovery of the receptors sensing infection. These studies on an insect model coincided with a revival of the field of innate immunity, and had an unanticipated impact on the biomedical field.},
keywords = {Allergy and Immunology, Animal, Animals, Antimicrobial Cationic Peptides, Antimicrobial peptides, history, Humans, IMD pathway, imler, Immunity, Innate, innate immunity, M3i, Models, Pattern recognition receptors, Signal Transduction, Toll-Like Receptors},
pubstate = {published},
tppubtype = {article}
}
Gubb David, Robertson Andrew S, Troxler Laurent, Reichhart Jean-Marc
Drosophila Serpins: Regulatory Cascades in Innate Immunity and Morphogenesis Book Section
In: Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity, pp. 207–227, Silverman GA and Lomas DA, London UK, 2007.
BibTeX | Tags: bioinformatic, innate immunity, M3i, Morphogenesis, regulatory Cascades, reichhart, Serpins
@incollection{gubb_drosophila_2007,
title = {Drosophila Serpins: Regulatory Cascades in Innate Immunity and Morphogenesis},
author = {David Gubb and Andrew S Robertson and Laurent Troxler and Jean-Marc Reichhart},
year = {2007},
date = {2007-01-01},
booktitle = {Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity},
pages = {207--227},
publisher = {Silverman GA and Lomas DA},
address = {London UK},
edition = {World Scientific Pub.},
keywords = {bioinformatic, innate immunity, M3i, Morphogenesis, regulatory Cascades, reichhart, Serpins},
pubstate = {published},
tppubtype = {incollection}
}