Publications
2019
Reina Giacomo, Zhao Li, Bianco Alberto, Komatsu Naoki
Chemical Functionalization of Nanodiamonds: Opportunities and Challenges Ahead Journal Article
In: Angewandte Chemie International Edition, vol. 58, no. 50, pp. 17918–17929, 2019, ISSN: 1521-3773.
Abstract | Links | BibTeX | Tags: carbon materials, diamond, Functionalization, I2CT, imaging, Team-Bianco, therapy
@article{reina_chemical_2019,
title = {Chemical Functionalization of Nanodiamonds: Opportunities and Challenges Ahead},
author = {Giacomo Reina and Li Zhao and Alberto Bianco and Naoki Komatsu},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201905997},
doi = {10.1002/anie.201905997},
issn = {1521-3773},
year = {2019},
date = {2019-01-01},
urldate = {2020-04-01},
journal = {Angewandte Chemie International Edition},
volume = {58},
number = {50},
pages = {17918--17929},
abstract = {Nanodiamond(ND)-based technologies are flourishing in a wide variety of fields spanning from electronics and optics to biomedicine. NDs are considered a family of nanomaterials with an sp3 carbon core and a variety of sizes, shapes, and surfaces. They show interesting physicochemical properties such as hardness, stiffness, and chemical stability. Additionally, they can undergo ad-hoc core and surface functionalization, which tailors them for the desired applications. Noteworthy, the properties of NDs and their surface chemistry are highly dependent on the synthetic method used to prepare them. In this Minireview, we describe the preparation of NDs from the materials-chemistry viewpoint. The different methodologies of synthesis, purification, and surface functionalization as well as biomedical applications are critically discussed. New synthetic approaches as well as limits and obstacles of NDs are presented and analyzed.},
keywords = {carbon materials, diamond, Functionalization, I2CT, imaging, Team-Bianco, therapy},
pubstate = {published},
tppubtype = {article}
}
2016
Jasim Dhifaf A, Boutin Herve, Fairclough Michael, Ménard-Moyon Cécilia, Prenant Christian, Bianco Alberto, Kostarelos Kostas
Thickness of functionalized graphene oxide sheets plays critical role in tissue accumulation and urinary excretion: A pilot PET/CT study Journal Article
In: Applied Materials Today, vol. 4, pp. 24–30, 2016, ISSN: 2352-9407.
Abstract | Links | BibTeX | Tags: carbon, I2CT, imaging, Nanomedicine, Pharmacokinetics, Pharmacology, Team-Bianco
@article{jasim_thickness_2016,
title = {Thickness of functionalized graphene oxide sheets plays critical role in tissue accumulation and urinary excretion: A pilot PET/CT study},
author = {Dhifaf A Jasim and Herve Boutin and Michael Fairclough and Cécilia Ménard-Moyon and Christian Prenant and Alberto Bianco and Kostas Kostarelos},
url = {http://www.sciencedirect.com/science/article/pii/S2352940716300099},
doi = {10.1016/j.apmt.2016.04.003},
issn = {2352-9407},
year = {2016},
date = {2016-09-01},
urldate = {2020-04-01},
journal = {Applied Materials Today},
volume = {4},
pages = {24--30},
abstract = {We have recently reported that administration of thin graphene oxide (GO) sheets in the systemic circulation of rodents leads to rapid urinary excretion for the majority of injected dose and accumulation by the reticuloendothelial system organs for the remaining dose. In this study, graphene oxide was functionalized with a chelating moiety (DOTA, (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid)) and labeled with [64Cu] for positron emission computed tomography (PET/CT) imaging. The thin functionalized graphene oxide material (f-GO-thin) consisted of a few layers (∼5nm) in thickness. Aging of the f-GO-thin material led to re-stacking of the flakes that resulted in materials of increased thickness (f-GO-thick) without altering their lateral dimension. These two types of f-GOs were comparatively studied pharmacologically to reveal the previously unexplored in vivo role of graphene oxide sheet thickness. Our results showed that a significantly larger fraction of the thicker GO sheets (47.5% of injected dose) remained within the body of living animals 24h after intravenous administration, residing mainly in the spleen and liver. The thinner GO sheets were predominantly (76.9% of injected dose) excreted through the glomerular filter into the urine. This pilot study provides an initial correlation between graphene-based material structure and pharmacological profile that is imperative towards understanding of how 2D structures behave in vivo to give information on potential biomedical applications.},
keywords = {carbon, I2CT, imaging, Nanomedicine, Pharmacokinetics, Pharmacology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2011
Al-Jamal Khuloud T, Nerl Hannah, Müller Karin H, Ali-Boucetta Hanene, Li Shouping, Haynes Peter D, Jinschek Joerg R, Prato Maurizio, Bianco Alberto, Kostarelos Kostas, Porter Alexandra E
Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging Journal Article
In: Nanoscale, vol. 3, no. 6, pp. 2627–2635, 2011, ISSN: 2040-3372.
Abstract | Links | BibTeX | Tags: carbon, Cell Line, Cell Membrane, Cytoplasm, Electron Microscope Tomography, Humans, I2CT, imaging, Macrophages, Nanotubes, Phagocytosis, Phagosomes, Team-Bianco, Three-Dimensional, tumor
@article{al-jamal_cellular_2011,
title = {Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging},
author = {Khuloud T Al-Jamal and Hannah Nerl and Karin H Müller and Hanene Ali-Boucetta and Shouping Li and Peter D Haynes and Joerg R Jinschek and Maurizio Prato and Alberto Bianco and Kostas Kostarelos and Alexandra E Porter},
doi = {10.1039/c1nr10080g},
issn = {2040-3372},
year = {2011},
date = {2011-06-01},
journal = {Nanoscale},
volume = {3},
number = {6},
pages = {2627--2635},
abstract = {Carbon nanotubes (CNTs) are being investigated for a variety of biomedical applications. Despite numerous studies, the pathways by which carbon nanotubes enter cells and their subsequent intracellular trafficking and distribution remain poorly determined. Here, we use 3-D electron tomography techniques that offer optimum enhancement of contrast between carbon nanotubes and the plasma membrane to investigate the mechanisms involved in the cellular uptake of shortened, functionalised multi-walled carbon nanotubes (MWNT-NH(3)(+)). Both human lung epithelial (A549) cells, that are almost incapable of phagocytosis and primary macrophages, capable of extremely efficient phagocytosis, were used. We observed that MWNT-NH(3)(+) were internalised in both phagocytic and non-phagocytic cells by any one of three mechanisms: (a) individually via membrane wrapping; (b) individually by direct membrane translocation; and (c) in clusters within vesicular compartments. At early time points following intracellular translocation, we noticed accumulation of nanotube material within various intracellular compartments, while a long-term (14-day) study using primary human macrophages revealed that MWNT-NH(3)(+) were able to escape vesicular (phagosome) entrapment by translocating directly into the cytoplasm.},
keywords = {carbon, Cell Line, Cell Membrane, Cytoplasm, Electron Microscope Tomography, Humans, I2CT, imaging, Macrophages, Nanotubes, Phagocytosis, Phagosomes, Team-Bianco, Three-Dimensional, tumor},
pubstate = {published},
tppubtype = {article}
}