Pathogenesis of bacterial infections and immunity

@article{,

title = {Anopheles gambiae miRNAs as actors of defence reaction against Plasmodium invasion},

author = { F. Winter and S. Edaye and A. Huttenhofer and C. Brunel},

year  = {2007},

date = {2007-01-01},

journal = {Nucleic Acids Res},

volume = {35},

number = {20},

pages = {6953-62},

abstract = {The path Plasmodium takes across the Anopheles midgut constitutes the major bottleneck during the malaria transmission cycle. In the present study, using a combination of shot-gun cloning and bioinformatic analysis, we have identified 18 miRNAs from Anopheles gambiae including three miRNAs unique to mosquito. Twelve of them are expressed ubiquitously across the body, independently of gender, while the other six exhibited an expression pattern restricted to the digestive system. Strikingly, the expression patterns of four miRNAs, including the three unique to mosquito, are affected by the presence of Plasmodium. We also show that knocking down Dicer1 and Ago1 mRNAs led to an increased sensitivity to Plasmodium infection. Altogether, these data support an involvement of miRNAs as new layers in the regulation of Anopheles defence reaction.},

note = {1362-4962 (Electronic) 

0305-1048 (Linking) 

Journal Article 

Research Support, Non-U.S. Gov't},

keywords = {*Gene, Animals, Anopheles, BRUNEL, Digestive, Expression, Female, gambiae/*genetics/*immunology/parasitology, Gene, III/genetics, Library, Male, MicroRNAs/*immunology, Plasmodium/*immunology, Profiling, Ribonuclease, silencing, System/immunology/metabolism/parasitology},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {[Estimation of DNA methylation level in nonendometrial uterus malignancies]},

author = { A. Popiela and M. S. Gabrys and J. Rabczynski and M. Panszczyk and G. Keith and W. Baranowski},

year  = {2002},

date = {2002-01-01},

journal = {Ginekol Pol},

volume = {73},

number = {11},

pages = {962-5},

abstract = {OBJECTIVES: Rebuilding of genome structure leads to many pathological states including neoplastic malignancies. Rebuilding often occurs as a process caused by disturbances in gene silencing mechanism. DNA methylation pattern is one of the most important mechanisms connected to gene's silencing. Estimation of DNA methylation level in nonendometrial uterine neoplastic tissues compared to normal endometrial samples was the aim of this study. DESIGN: It was to be shown, that changes in methylation rate in promotory regions could lead to carcinogenesis in particular cell. Authors describe an analysis of DNA methylation level in nonendometrial neoplastic uterine tissues compared to DNA methylation level in normal endometrium. MATERIALS AND METHODS: Tissue samples from 9 women with tumor mixtus mesodermalis were collected. 12 samples were normal endometrium-control group. DNA was isolated from tissues and than we performed an estimation of DNA methylation levels. Than we performed a statistical analysis of results. RESULTS: The median DNA methylation level was significantly higher in neoplastic tissues than in normal endometrium. CONCLUSIONS: Authors conclude, that DNA methylation level is higher in neoplastic tissues, but does not correlate with clinical stage of the disease.},

note = {0017-0011 

Journal Article},

keywords = {*DNA, 80, Abstract, Age, Aged, and, Case-Control, English, Expression, Factors, Female, Gene, Human, Mesodermal/genetics/*metabolism, Methylation, Middle, Mixed, Neoplasms/genetics/*metabolism, Neoplastic, over, Regulation, silencing, Studies, tumor, Uterine},

pubstate = {published},

tppubtype = {article}

}