Publications
2014
Marangon Iris, Ménard‐Moyon Cécilia, Kolosnjaj‐Tabi Jelena, Béoutis Marie Lys, Lartigue Lénaic, Alloyeau Damien, Pach Elzbieta, Ballesteros Belén, Autret Gwennhael, Ninjbadgar Tsedev, Brougham Dermot F, Bianco Alberto, Gazeau Florence
Covalent Functionalization of Multi-walled Carbon Nanotubes with a Gadolinium Chelate for Efficient T1-Weighted Magnetic Resonance Imaging Journal Article
In: Advanced Functional Materials, vol. 24, no. 45, pp. 7173–7186, 2014, ISSN: 1616-3028.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, contrast agents, I2CT, magnetic resonance imaging, Nanomedicine, Team-Bianco
@article{marangon_covalent_2014,
title = {Covalent Functionalization of Multi-walled Carbon Nanotubes with a Gadolinium Chelate for Efficient T1-Weighted Magnetic Resonance Imaging},
author = {Iris Marangon and Cécilia Ménard‐Moyon and Jelena Kolosnjaj‐Tabi and Marie Lys Béoutis and Lénaic Lartigue and Damien Alloyeau and Elzbieta Pach and Belén Ballesteros and Gwennhael Autret and Tsedev Ninjbadgar and Dermot F Brougham and Alberto Bianco and Florence Gazeau},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201402234},
doi = {10.1002/adfm.201402234},
issn = {1616-3028},
year = {2014},
date = {2014-01-01},
urldate = {2020-04-02},
journal = {Advanced Functional Materials},
volume = {24},
number = {45},
pages = {7173--7186},
abstract = {Given the promise of carbon nanotubes (CNTs) for photothermal therapy, drug delivery, tissue engineering, and gene therapy, there is a need for non-invasive imaging methods to monitor CNT distribution and fate in the body. In this study, non-ionizing whole-body high field magnetic resonance imaging (MRI) is used to follow the distribution of water-dispersible non-toxic functionalized CNTs administrated intravenously to mice. Oxidized CNTs are endowed with positive MRI contrast properties by covalent functionalization with the chelating ligand diethylenetriaminepentaacetic dianhydride (DTPA), followed by chelation to Gd3+. The structural and magnetic properties, MR relaxivities, cellular uptake, and application for MRI cell imaging of Gd-CNTs in comparison to the precursor oxidized CNTs are evaluated. Despite the intrinsic T2 contrast of oxidized CNTs internalized in macrophages, the anchoring of paramagnetic gadolinium onto the nanotube sidewall allows efficient T1 contrast and MR signal enhancement, which is preserved after CNT internalization by cells. Hence, due to their high dispersibility, Gd-CNTs have the potential to produce positive contrast in vivo following injection into the bloodstream. The uptake of Gd-CNTs in the liver and spleen is assessed using MRI, while rapid renal clearance of extracellular Gd-CNTs is observed, confirming the evidences of other studies using different imaging modalities.},
keywords = {Carbon nanotubes, contrast agents, I2CT, magnetic resonance imaging, Nanomedicine, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2013
Dumortier Hélène
When carbon nanotubes encounter the immune system: desirable and undesirable effects Journal Article
In: Advanced Drug Delivery Reviews, vol. 65, no. 15, pp. 2120–2126, 2013, ISSN: 1872-8294.
Abstract | Links | BibTeX | Tags: Animals, Biomedical application, carbon, Carbon nanotubes, Dumortier, Environmental Exposure, Functionalization, Humans, I2CT, Immune cell activation, Immune System, inflammation, Inhalation Exposure, Lymphocyte, Macrophage, Nanotubes, Occupational Exposure, Team-Dumortier, Toxicity
@article{dumortier_when_2013,
title = {When carbon nanotubes encounter the immune system: desirable and undesirable effects},
author = {Hélène Dumortier},
doi = {10.1016/j.addr.2013.09.005},
issn = {1872-8294},
year = {2013},
date = {2013-01-01},
journal = {Advanced Drug Delivery Reviews},
volume = {65},
number = {15},
pages = {2120--2126},
abstract = {The role of our immune system is to bring efficient protection against invasion by foreign elements, not only pathogens but also any material it may be in contact with. Nanoparticles may enter the body and encounter the immune system either intentionally (e.g. administration for biomedical application) or not (e.g. respiratory occupational exposure). Therefore, it is of fundamental importance to get a thorough knowledge of the way they interact with immune cells and all related consequences. Among nanomaterials, carbon nanotubes (CNTs) are of special interest because of their tremendous field of applications. Consequently, their increasing production, processing and eventual incorporation into new types of composites and/or into biological systems have raised fundamental issues regarding their potential impact on health. This review aims at giving an overview of the known desirable and undesirable effects of CNTs on the immune system, i.e. beneficial modulation of immune cells by CNTs engineered for biomedical applications versus toxicity, inflammation and unwanted immune reactions triggered by CNTs themselves.},
keywords = {Animals, Biomedical application, carbon, Carbon nanotubes, Dumortier, Environmental Exposure, Functionalization, Humans, I2CT, Immune cell activation, Immune System, inflammation, Inhalation Exposure, Lymphocyte, Macrophage, Nanotubes, Occupational Exposure, Team-Dumortier, Toxicity},
pubstate = {published},
tppubtype = {article}
}
2009
Gaillard Claire, Cellot Giada, Li Shouping, Toma Francesca Maria, Dumortier Hélène, Spalluto Giampiero, Cacciari Barbara, Prato Maurizio, Ballerini Laura, Bianco Alberto
Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality Journal Article
In: Advanced Materials, vol. 21, no. 28, pp. 2903–2908, 2009, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco
@article{gaillard_carbon_2009,
title = {Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality},
author = {Claire Gaillard and Giada Cellot and Shouping Li and Francesca Maria Toma and Hélène Dumortier and Giampiero Spalluto and Barbara Cacciari and Maurizio Prato and Laura Ballerini and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200900050},
doi = {10.1002/adma.200900050},
issn = {1521-4095},
year = {2009},
date = {2009-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {21},
number = {28},
pages = {2903--2908},
abstract = {Water-soluble carbon nanotubes functionalized with cell-adhesion peptides do not affect the viability of different cell types, including Jurkat cells, splenocytes, and neurons. They also do not modify the neuronal morphology and basic functions, thus representing a promising candidate for the exploitation of novel drug-delivery systems or for designing a new generation of self-assembling nerve bridges.},
keywords = {Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2008
Lacerda L, Soundararajan A, Singh R, Pastorin G, Al‐Jamal K T, Turton J, Frederik P, Herrero M A, Li S, Bao A, Emfietzoglou D, Mather S, Phillips W T, Prato M, Bianco A, Goins B, Kostarelos K
Dynamic Imaging of Functionalized Multi-Walled Carbon Nanotube Systemic Circulation and Urinary Excretion Journal Article
In: Advanced Materials, vol. 20, no. 2, pp. 225–230, 2008, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: Biomedical applications, Carbon nanotubes, I2CT, multiwalled, Nanomaterials, Team-Bianco
@article{lacerda_dynamic_2008,
title = {Dynamic Imaging of Functionalized Multi-Walled Carbon Nanotube Systemic Circulation and Urinary Excretion},
author = {L Lacerda and A Soundararajan and R Singh and G Pastorin and K T Al‐Jamal and J Turton and P Frederik and M A Herrero and S Li and A Bao and D Emfietzoglou and S Mather and W T Phillips and M Prato and A Bianco and B Goins and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200702334},
doi = {10.1002/adma.200702334},
issn = {1521-4095},
year = {2008},
date = {2008-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {20},
number = {2},
pages = {225--230},
abstract = {Intravenously administered multi-walled carbon nanotubes, functionalized with DTPA and radiolabeled with Indium-111, were dynamically tracked in vivo using a microSingle Photon Emission Tomography scanner. Imaging showed that nanotubes enter the systemic blood circulation and within 5 min begin to permeate through the renal glomerular filtration system into the bladder.},
keywords = {Biomedical applications, Carbon nanotubes, I2CT, multiwalled, Nanomaterials, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2006
Campidelli Stéphane, Klumpp Cédric, Bianco Alberto, Guldi Dirk M, Prato Maurizio
Functionalization of CNT: synthesis and applications in photovoltaics and biology Journal Article
In: Journal of Physical Organic Chemistry, vol. 19, no. 8-9, pp. 531–539, 2006, ISSN: 1099-1395.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, Cells, Drug delivery, electron transfer, Functionalization, I2CT, Peptides, photovoltaic, Team-Bianco, Toxicity, Vectors
@article{campidelli_functionalization_2006,
title = {Functionalization of CNT: synthesis and applications in photovoltaics and biology},
author = {Stéphane Campidelli and Cédric Klumpp and Alberto Bianco and Dirk M Guldi and Maurizio Prato},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/poc.1052},
doi = {10.1002/poc.1052},
issn = {1099-1395},
year = {2006},
date = {2006-01-01},
urldate = {2020-03-31},
journal = {Journal of Physical Organic Chemistry},
volume = {19},
number = {8-9},
pages = {531--539},
abstract = {Here, we review part of the work carried out in our laboratories on carbon nanotube functionalization. Both covalent (sidewall derivatization) and non-covalent (using π-π interactions) functionalization have been used to solubilize carbon nanotubes (NTs). The combination of NTs with various electron donors, mainly using the supramolecular approach, led to a new generation of donor-acceptor nanohybrids which can be used for the development of carbon-based photovoltaic cells. Covalent functionalization has been successfully applied for preparation of water soluble nanotubes and further derivatization of the nanotubes with bioactive molecules hold great promise for application in drug, vaccine and gene delivery. Copyright © 2006 John Wiley & Sons, Ltd.},
keywords = {Carbon nanotubes, Cells, Drug delivery, electron transfer, Functionalization, I2CT, Peptides, photovoltaic, Team-Bianco, Toxicity, Vectors},
pubstate = {published},
tppubtype = {article}
}
Lacerda L, Pastorin G, Wu W, Prato M, Bianco A, Kostarelos K
In: Advanced Functional Materials, vol. 16, no. 14, pp. 1839–1846, 2006, ISSN: 1616-3028.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, DNA, I2CT, Luminescence, multiwalled, single-walled, Team-Bianco
@article{lacerda_luminescence_2006,
title = {Luminescence of Functionalized Carbon Nanotubes as a Tool to Monitor Bundle Formation and Dissociation in Water: The Effect of Plasmid-DNA Complexation},
author = {L Lacerda and G Pastorin and W Wu and M Prato and A Bianco and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.200500569},
doi = {10.1002/adfm.200500569},
issn = {1616-3028},
year = {2006},
date = {2006-01-01},
urldate = {2020-03-31},
journal = {Advanced Functional Materials},
volume = {16},
number = {14},
pages = {1839--1846},
abstract = {Functionalized carbon nanotubes (f-CNTs) are explored as novel nanomaterials for biomedical applications. UV-vis luminescence of aqueous dispersions of CNT–NH3+ and CNT–NH–Ac (NH–Ac: acetamido) is observed using standard laboratory spectrophotometric instrumentation, and the measured fluorescence intensity is correlated with the aggregation state of the f-CNTs: a high intensity indicates improved f-CNT individualization and dispersion, while a decrease in fluorescence intensity indicates a higher degree of nanotube aggregation and bundling as a result of varying the sodium dodecyl sulfate (SDS) concentrations and pH in the aqueous phase. Moreover, utilization of this relationship between fluorescence intensity and the state of f-CNT aggregation is carried out to elucidate the interactions between f-CNTs and gene-encoding plasmid DNA (pDNA). pDNA is shown to interact with CNT–NH3+ primarily through electrostatic interactions that lead concomitantly to a higher degree of f-CNT bundling. The CNT–NH3+/pDNA interactions are successfully competed by SDS/f-CNT surface interactions, resulting in the displacement of pDNA. These studies provide exemplification of the use of fluorescence spectrophotometry to accurately describe the aggregation state of water-soluble f-CNTs. Characterization of the complexes between pDNA and f-CNTs elucidates the opportunities and limitations of such supramolecular systems as potential vectors for gene transfer.},
keywords = {Carbon nanotubes, DNA, I2CT, Luminescence, multiwalled, single-walled, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2005
Kostarelos K, Lacerda L, Partidos C D, Prato M, Bianco A
Carbon nanotube-mediated delivery of peptides and genes to cells: translating nanobiotechnology to therapeutics Journal Article
In: Journal of Drug Delivery Science and Technology, vol. 15, no. 1, pp. 41–47, 2005, ISSN: 1773-2247.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, gene delivery, gene therapy, I2CT, Nanomedicine, Peptide delivery, Team-Bianco, Vaccination
@article{kostarelos_carbon_2005,
title = {Carbon nanotube-mediated delivery of peptides and genes to cells: translating nanobiotechnology to therapeutics},
author = {K Kostarelos and L Lacerda and C D Partidos and M Prato and A Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S1773224705500054},
doi = {10.1016/S1773-2247(05)50005-4},
issn = {1773-2247},
year = {2005},
date = {2005-01-01},
urldate = {2020-03-31},
journal = {Journal of Drug Delivery Science and Technology},
volume = {15},
number = {1},
pages = {41--47},
abstract = {During the last few years, there has been a tremendous amount of optimism and expectation about nanotechnology and its impact on various fields including medicine and pharmaceutical development. One of the most promising materials being developed during the nanotechnological renaissance we are currently experiencing is the carbon nanotube. Before any biology-related application can even be envisaged, the aqueous solubility of carbon nanotubes has to be resolved. Recently, a variety of methodologies have been proposed which lead to biologically compatible carbon nanotubes. Covalent functionalization of their surface is one methodology, allowing the first attempts towards applications in the field of nanomedicine. The possibility of incorporating functionalized carbon nanotubes into cells and the biological milieu offers numerous advantages for potential applications in biology and pharmacology. One of the most promising is their utilization as a new carrier system for the delivery of therapeutic molecules. In the present article, the first attempts to transform carbon nanotubes from biologically incompatible nanomaterials to biologically relevant components of advanced therapeutics and the ensuing novel structures obtained in our laboratories are presented.},
keywords = {Carbon nanotubes, gene delivery, gene therapy, I2CT, Nanomedicine, Peptide delivery, Team-Bianco, Vaccination},
pubstate = {published},
tppubtype = {article}
}
2004
Pantarotto Davide, Singh Ravi, McCarthy David, Erhardt Mathieu, Briand Jean-Paul, Prato Maurizio, Kostarelos Kostas, Bianco Alberto
Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery Journal Article
In: Angewandte Chemie International Edition, vol. 43, no. 39, pp. 5242–5246, 2004, ISSN: 1521-3773.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, gene delivery, I2CT, plasmid DNA, supramolecular chemistry, Team-Bianco
@article{pantarotto_functionalized_2004,
title = {Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery},
author = {Davide Pantarotto and Ravi Singh and David McCarthy and Mathieu Erhardt and Jean-Paul Briand and Maurizio Prato and Kostas Kostarelos and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.200460437},
doi = {10.1002/anie.200460437},
issn = {1521-3773},
year = {2004},
date = {2004-01-01},
urldate = {2020-03-31},
journal = {Angewandte Chemie International Edition},
volume = {43},
number = {39},
pages = {5242--5246},
abstract = {Genetic vaccination and gene therapy research could benefit from the application of carbon nanotubes. Functionalized, positively charged, water-soluble carbon nanotubes are able to penetrate into cells (see figure) and can transport plasmid DNA by formation of noncovalent DNA–nanotube complexes. Such nanotubes can be used as novel nonviral delivery systems for gene transfer.},
keywords = {Carbon nanotubes, gene delivery, I2CT, plasmid DNA, supramolecular chemistry, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2003
Bianco A, Prato M
Can Carbon Nanotubes be Considered Useful Tools for Biological Applications? Journal Article
In: Advanced Materials, vol. 15, no. 20, pp. 1765–1768, 2003, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, Functionalization, I2CT, Team-Bianco
@article{bianco_can_2003,
title = {Can Carbon Nanotubes be Considered Useful Tools for Biological Applications?},
author = {A Bianco and M Prato},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200301646},
doi = {10.1002/adma.200301646},
issn = {1521-4095},
year = {2003},
date = {2003-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {15},
number = {20},
pages = {1765--1768},
abstract = {Carbon nanotubes can be made soluble in both organic solvents and in aqueous solutions by organic functionalization. In particular, soluble carbon nanotubes can be further derivatized by coupling with amino acids and bioactive peptides. Immobilization of peptides to the external walls of carbon nanotubes may find interesting applications in diagnostics, vaccine and drug delivery, or multipresentation of bioactive molecules.},
keywords = {Carbon nanotubes, Functionalization, I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}