Pathogenesis of bacterial infections and immunity

@article{,

title = {Rapid selection of aminoacyl-tRNAs based on biotinylation of alpha-NH2 group of charged amino acids},

author = {J Putz and J Wientges and M Sissler and R Giege and C Florentz and A Schwienhorst},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9162902},

isbn = {9162902},

year  = {1997},

date = {1997-01-01},

journal = {Nucleic Acids Res},

volume = {25},

number = {9},

pages = {1862-1863},

abstract = {A rapid selection procedure to separate low amounts of aminoacylated tRNAs from large pools of inactive variants is described. The procedure involves a three-step protocol. After initial aminoacylation of a tRNA pool, N-hydroxysuccinimide ester chemistry is applied to biotinylate the alpha-NH2 group of the amino acid bound to the 3'-end of a tRNA. The biotin tag is used to capture the derivatized tRNAs on streptavidin-conjugated magnetic beads. Variants bound to the solid phase can be amplified by RT-PCR and transcription, providing tRNAs for subsequent selection rounds.},

note = {0305-1048 

Journal Article},

keywords = {Amino Acids/*chemistry  Biotin/*chemistry  Polymerase Chain Reaction  RNA, Amino Acyl/chemistry/*isolation & purification  Support, FLORENTZ, Non-U.S. Gov't, SISSLER, Transfer, Unité ARN},

pubstate = {published},

tppubtype = {article}

}