Pathogenesis of bacterial infections and immunity

@article{russier_oxidative_2011,

title = {Oxidative biodegradation of single- and multi-walled carbon nanotubes},

author = {Julie Russier and Cécilia Ménard-Moyon and Enrica Venturelli and Edmond Gravel and Gabriele Marcolongo and Moreno Meneghetti and Eric Doris and Alberto Bianco},

doi = {10.1039/c0nr00779j},

issn = {2040-3372},

year  = {2011},

date = {2011-03-01},

journal = {Nanoscale},

volume = {3},

number = {3},

pages = {893--896},

abstract = {In this study we compare the biodegradation of both single-walled (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) using two different oxidative conditions. In particular, we demonstrate that oxidized multi-walled carbon nanotubes are highly degraded, although not to completeness when treated with horseradish peroxidase (HRP) in the presence of hydrogen peroxide.},

keywords = {Absorbable Implants, Biocompatible Materials, Body Fluids, carbon, Horseradish Peroxidase, Hydrogen Peroxide, I2CT, Macromolecular Substances, Materials Testing, Molecular Conformation, Nanotubes, Oxidation-Reduction, Particle Size, Surface Properties, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}

@article{singh_organic_2009,

title = {Organic functionalisation and characterisation of single-walled carbon nanotubes},

author = {Prabhpreet Singh and Stéphane Campidelli and Silvia Giordani and Davide Bonifazi and Alberto Bianco and Maurizio Prato},

doi = {10.1039/b518111a},

issn = {1460-4744},

year  = {2009},

date = {2009-08-01},

journal = {Chemical Society Reviews},

volume = {38},

number = {8},

pages = {2214--2230},

abstract = {Since carbon nanotubes (CNTs) display unique structures and remarkable physical properties, a variety of applications have emerged in both materials and life sciences. In terms of applications, the functionalisation of nanotubes is extremely important, as it increases their solubility and processability, and combines the unique properties of single-walled carbon nanotubes (SWCNTs) with those of other classes of materials. A number of methods have been developed, which can be divided into two major approaches: (1) non-covalent supramolecular modifications, and (2) covalent functionalisation. In this tutorial review, we survey the covalent modification of SWCNTs with organic moieties, and illustrate the major analytical techniques routinely used to characterise the functionalised materials.},

keywords = {Alkylation, carbon, Esterification, Free Radicals, Halogenation, I2CT, Microscopy, Nanotubes, Oxidation-Reduction, Spectrum Analysis, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}

@article{marega_diffusion-ordered_2009,

title = {Diffusion-ordered NMR spectroscopy in the structural characterization of functionalized carbon nanotubes},

author = {Riccardo Marega and Vincent Aroulmoji and Francesca Dinon and Lisa Vaccari and Silvia Giordani and Alberto Bianco and Erminio Murano and Maurizio Prato},

doi = {10.1021/ja902728w},

issn = {1520-5126},

year  = {2009},

date = {2009-07-01},

journal = {Journal of the American Chemical Society},

volume = {131},

number = {25},

pages = {9086--9093},

abstract = {The emerging applications of functionalized carbon nanotubes (CNTs) in various research domains necessitate the use of many different analytical techniques to confirm their structural modifications in a fast and reliable manner. Thus far, NMR spectroscopy has not been among the main tools for characterization of organically modified carbon nanostructures. (1)H analysis is limited because the signals in these derivatives are typically weak and broad, resulting in uncertainties of a few parts per million, and because of the strong interference of residual solvent signals. To overcome these limitations, we investigated the applicability of proton NMR spectroscopy based on gradient-edited diffusion pulse sequences (1D diffusion-ordered spectroscopy, DOSY) in the characterization of CNT derivatives. In general, diffusion NMR experiments allow the separation of NMR signals of different species present in a mixture, according to their own diffusion coefficients, merging spectroscopy information with size analysis. In the present study, a selected set of CNT derivatives was synthesized and analyzed using 1D DOSY experiments by applying strong magnetic field gradients (up to 42.6 G cm(-1)). Colorimetric tests (i.e., Kaiser test) and TGA analysis support the NMR findings, which are related to isolated and/or bundled short SWNTs, on the basis of TEM and AFM characterization. The overall results show that the diffusion-based NMR spectroscopy is a fast and promising approach for the characterization of covalently modified CNT derivatives.},

keywords = {carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Oxidation-Reduction, Surface Properties, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}

@article{hauquier_carbon_2006,

title = {Carbon nanotube-functionalized silicon surfaces with efficient redox communication},

author = {Fanny Hauquier and Giorgia Pastorin and Philippe Hapiot and Maurizio Prato and Alberto Bianco and Bruno Fabre},

doi = {10.1039/b610559a},

issn = {1359-7345},

year  = {2006},

date = {2006-11-01},

journal = {Chemical Communications (Cambridge, England)},

number = {43},

pages = {4536--4538},

abstract = {Sidewall functionalized multi-walled carbon nanotubes can be covalently bound parallel to a silicon surface via a self-assembled acid-terminated monolayer used as an organic molecular glue.},

keywords = {carbon, I2CT, Nanotubes, Oxidation-Reduction, Silicon, Surface Properties, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}

@article{dumortier_functionalized_2006,

title = {Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells},

author = {Hélène Dumortier and Stéphanie Lacotte and Giorgia Pastorin and Riccardo Marega and Wei Wu and Davide Bonifazi and Jean-Paul Briand and Maurizio Prato and Sylviane Muller and Alberto Bianco},

doi = {10.1021/nl061160x},

issn = {1530-6984},

year  = {2006},

date = {2006-07-01},

journal = {Nano Letters},

volume = {6},

number = {7},

pages = {1522--1528},

abstract = {Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.},

keywords = {Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, Dumortier, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco, Team-Dumortier},

pubstate = {published},

tppubtype = {article}

}

@article{dumortier_functionalized_2006b,

title = {Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells},

author = {Hélène Dumortier and Stéphanie Lacotte and Giorgia Pastorin and Riccardo Marega and Wei Wu and Davide Bonifazi and Jean-Paul Briand and Maurizio Prato and Sylviane Muller and Alberto Bianco},

doi = {10.1021/nl061160x},

issn = {1530-6984},

year  = {2006},

date = {2006-07-01},

journal = {Nano Letters},

volume = {6},

number = {7},

pages = {1522--1528},

abstract = {Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.},

keywords = {Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}

@article{brough_60fullerene-pyrrolidine-n-oxides_2006,

title = {[60]fullerene-pyrrolidine-N-oxides},

author = {Peter Brough and Cedric Klumpp and Alberto Bianco and Stephane Campidelli and Maurizio Prato},

doi = {10.1021/jo052388s},

issn = {0022-3263},

year  = {2006},

date = {2006-03-01},

journal = {The Journal of Organic Chemistry},

volume = {71},

number = {5},

pages = {2014--2020},

abstract = {Eight members of a new family of fullerene derivatives, [60]fulleropyrrolidine-N-oxides, have been synthesized and characterized. Facile oxidation, by a peracid, of the parent [60]fulleropyrrolidine gave clean conversions into the product molecules, in which the tertiary amine is transformed into a quaternary amine bearing an oxygen atom. The reaction is very selective, favoring the nitrogen atom of the pyrrolidine ring in preference to epoxidation of the fullerene cage. The 1H NMR shows an AB quartet splitting pattern, characteristic of nonequivalent hydrogens in the pyrrolidine ring and at a chemical shift displacement of 0.8 ppm downfield. Other methods of characterization are described, including MS, differential scanning calorimetry, thermogravimetric analysis, HPLC, UV/vis, and IR. Conclusive evidence for the formation of an N-oxide moiety is provided by the synthesis, oxidation, and NMR characterization of a novel [60]fulleropyrrolidine containing a 15N isotope, showing an 85 ppm downfield heteroatom chemical shift. Preliminary details of the effects of substitution on the reactivity of the pyrrolidine ring are also reported.},

keywords = {Chromatography, Fullerenes, High Pressure Liquid, I2CT, Nitrogen, Oxidation-Reduction, Oxides, Pyrrolidines, Spectrum Analysis, Team-Bianco},

pubstate = {published},

tppubtype = {article}

}

@article{de_marco_solubility_2004,

title = {The solubility and stability of recombinant proteins are increased by their fusion to NusA},

author = {Valeria De Marco and Gunter Stier and Stephanie A Blandin and Ario de Marco},

doi = {10.1016/j.bbrc.2004.07.189},

issn = {0006-291X},

year  = {2004},

date = {2004-01-01},

journal = {Biochem. Biophys. Res. Commun.},

volume = {322},

number = {3},

pages = {766--771},

abstract = {The new bacterial vector pETM60 enables the expression of His-tagged recombinant proteins fused to the C-terminus of NusA through a TEV protease recognition sequence. Three sequences coding for two protein domains (Xklp3A and Tep3Ag) and one membrane-bound viral protein (E8R) could not be expressed in a soluble form in bacteria. Their GST-fusions were mostly soluble but quickly degraded during purification. The same sequences cloned in pETM60 were efficiently purified by metal affinity and recovered soluble after the removal of the fusion partner. The NusA-fused constructs enabled to yield 13-20mg of fusion protein per litre of culture and 2.5-5mg of pure protein per litre of culture. Structural analysis indicated that the purified proteins were monodispersed and correctly folded. NusA has been used to raise antibodies that have been successfully used for Western blot and immunoprecipitation of NusA fusion proteins.},

keywords = {blandin, Drug Stability, Escherichia coli Proteins, Genetic Vectors, Glutathione Transferase, Kinetics, M3i, Oxidation-Reduction, Peptide Elongation Factors, Recombinant Fusion Proteins, Recombinant Proteins, Solubility, Transcription Factors, Transcriptional Elongation Factors},

pubstate = {published},

tppubtype = {article}

}