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2008
Prato Maurizio, Kostarelos Kostas, Bianco Alberto
Functionalized carbon nanotubes in drug design and discovery Journal Article
In: Accounts of Chemical Research, vol. 41, no. 1, pp. 60–68, 2008, ISSN: 1520-4898.
Abstract | Links | BibTeX | Tags: Animals, carbon, Communicable Diseases, Drug Carriers, Drug Design, Genetic Therapy, Humans, I2CT, Immunization, Nanotubes, Neoplasms, Team-Bianco
@article{prato_functionalized_2008,
title = {Functionalized carbon nanotubes in drug design and discovery},
author = {Maurizio Prato and Kostas Kostarelos and Alberto Bianco},
doi = {10.1021/ar700089b},
issn = {1520-4898},
year = {2008},
date = {2008-01-01},
journal = {Accounts of Chemical Research},
volume = {41},
number = {1},
pages = {60--68},
abstract = {Carbon nanotubes (CNTs) have been proposed and actively explored as multipurpose innovative carriers for drug delivery and diagnostic applications. Their versatile physicochemical features enable the covalent and noncovalent introduction of several pharmaceutically relevant entities and allow for rational design of novel candidate nanoscale constructs for drug development. CNTs can be functionalized with different functional groups to carry simultaneously several moieties for targeting, imaging, and therapy. Among the most interesting examples of such multimodal CNT constructs described in this Account is one carrying a fluorescein probe together with the antifungal drug amphotericin B or fluorescein and the antitumor agent methotrexate. The biological action of the drug in these cases is retained or, as in the case of amphotericin B constructs, enhanced, while CNTs are able to reduce the unwanted toxicity of the drug administered alone. Ammonium-functionalized CNTs can also be considered very promising vectors for gene-encoding nucleic acids. Indeed, we have formed stable complexes between cationic CNTs and plasmid DNA and demonstrated the enhancement of the gene therapeutic capacity in comparison to DNA alone. On the other hand, CNTs conjugated with antigenic peptides can be developed as a new and effective system for synthetic vaccine applications. What makes CNTs quite unique is their ability, first shown by our groups in 2004, to passively cross membranes of many different types of cells following a translocation mechanism that has been termed the nanoneedle mechanism. In that way, CNTs open innumerable possibilities for future drug discovery based on intracellular targets that have been hard to reach until today. Moreover, adequately functionalized CNTs as those shown in this Account can be rapidly eliminated from the body following systemic administration offering further encouragment for their development. CNT excretion rates and accumulation in organs and any reactivity with the immune system will determine the CNT safety profile and, consequently, any further pharmaceutical development. Caution is advised about the need for systematic data on the long-term fate of these very interesting and versatile nano-objects in correlation with the type of CNT material used. CNTs are gradually plyaing a bigger and more important role in the emerging field of nanomedicine; however, we need to guarantee that the great opportunities they offer will be translated into feasible and safe constructs to be included in drug discovery and development pipelines.},
keywords = {Animals, carbon, Communicable Diseases, Drug Carriers, Drug Design, Genetic Therapy, Humans, I2CT, Immunization, Nanotubes, Neoplasms, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) have been proposed and actively explored as multipurpose innovative carriers for drug delivery and diagnostic applications. Their versatile physicochemical features enable the covalent and noncovalent introduction of several pharmaceutically relevant entities and allow for rational design of novel candidate nanoscale constructs for drug development. CNTs can be functionalized with different functional groups to carry simultaneously several moieties for targeting, imaging, and therapy. Among the most interesting examples of such multimodal CNT constructs described in this Account is one carrying a fluorescein probe together with the antifungal drug amphotericin B or fluorescein and the antitumor agent methotrexate. The biological action of the drug in these cases is retained or, as in the case of amphotericin B constructs, enhanced, while CNTs are able to reduce the unwanted toxicity of the drug administered alone. Ammonium-functionalized CNTs can also be considered very promising vectors for gene-encoding nucleic acids. Indeed, we have formed stable complexes between cationic CNTs and plasmid DNA and demonstrated the enhancement of the gene therapeutic capacity in comparison to DNA alone. On the other hand, CNTs conjugated with antigenic peptides can be developed as a new and effective system for synthetic vaccine applications. What makes CNTs quite unique is their ability, first shown by our groups in 2004, to passively cross membranes of many different types of cells following a translocation mechanism that has been termed the nanoneedle mechanism. In that way, CNTs open innumerable possibilities for future drug discovery based on intracellular targets that have been hard to reach until today. Moreover, adequately functionalized CNTs as those shown in this Account can be rapidly eliminated from the body following systemic administration offering further encouragment for their development. CNT excretion rates and accumulation in organs and any reactivity with the immune system will determine the CNT safety profile and, consequently, any further pharmaceutical development. Caution is advised about the need for systematic data on the long-term fate of these very interesting and versatile nano-objects in correlation with the type of CNT material used. CNTs are gradually plyaing a bigger and more important role in the emerging field of nanomedicine; however, we need to guarantee that the great opportunities they offer will be translated into feasible and safe constructs to be included in drug discovery and development pipelines.
Lacerda L, Soundararajan A, Singh R, Pastorin G, Al‐Jamal K T, Turton J, Frederik P, Herrero M A, Li S, Bao A, Emfietzoglou D, Mather S, Phillips W T, Prato M, Bianco A, Goins B, Kostarelos K
Dynamic Imaging of Functionalized Multi-Walled Carbon Nanotube Systemic Circulation and Urinary Excretion Journal Article
In: Advanced Materials, vol. 20, no. 2, pp. 225–230, 2008, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: Biomedical applications, Carbon nanotubes, I2CT, multiwalled, Nanomaterials, Team-Bianco
@article{lacerda_dynamic_2008,
title = {Dynamic Imaging of Functionalized Multi-Walled Carbon Nanotube Systemic Circulation and Urinary Excretion},
author = {L Lacerda and A Soundararajan and R Singh and G Pastorin and K T Al‐Jamal and J Turton and P Frederik and M A Herrero and S Li and A Bao and D Emfietzoglou and S Mather and W T Phillips and M Prato and A Bianco and B Goins and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200702334},
doi = {10.1002/adma.200702334},
issn = {1521-4095},
year = {2008},
date = {2008-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {20},
number = {2},
pages = {225--230},
abstract = {Intravenously administered multi-walled carbon nanotubes, functionalized with DTPA and radiolabeled with Indium-111, were dynamically tracked in vivo using a microSingle Photon Emission Tomography scanner. Imaging showed that nanotubes enter the systemic blood circulation and within 5 min begin to permeate through the renal glomerular filtration system into the bladder.},
keywords = {Biomedical applications, Carbon nanotubes, I2CT, multiwalled, Nanomaterials, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Intravenously administered multi-walled carbon nanotubes, functionalized with DTPA and radiolabeled with Indium-111, were dynamically tracked in vivo using a microSingle Photon Emission Tomography scanner. Imaging showed that nanotubes enter the systemic blood circulation and within 5 min begin to permeate through the renal glomerular filtration system into the bladder.
Lacotte Stéphanie, García Ainara, Décossas Marion, Al‐Jamal Wafa' T, Li Shouping, Kostarelos Kostas, Muller Sylviane, Prato Maurizio, Dumortier Hélène, Bianco Alberto
Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells Journal Article
In: Advanced Materials, vol. 20, no. 12, pp. 2421–2426, 2008, ISSN: 1521-4095.
Abstract | Links | BibTeX | Tags: carbon nanostructures, Cells, Cytotoxicity, Drug delivery, I2CT, Team-Bianco
@article{lacotte_interfacing_2008,
title = {Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells},
author = {Stéphanie Lacotte and Ainara García and Marion Décossas and Wafa' T Al‐Jamal and Shouping Li and Kostas Kostarelos and Sylviane Muller and Maurizio Prato and Hélène Dumortier and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200702753},
doi = {10.1002/adma.200702753},
issn = {1521-4095},
year = {2008},
date = {2008-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {20},
number = {12},
pages = {2421--2426},
abstract = {Functionalized carbon nanohorns (f-CNH) are uptaken by macrophages, without affecting cell viability. f-CNH induce the production of reactive oxygen species and of pro-inflammatory cytokines. The level of inflammation, although moderate, should be taken into consideration when using f-CNH for drug delivery. However, it could be exploited as an intrinsic f-CNH adjuvant function for biomedical applications requiring some activation of the immune system.},
keywords = {carbon nanostructures, Cells, Cytotoxicity, Drug delivery, I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalized carbon nanohorns (f-CNH) are uptaken by macrophages, without affecting cell viability. f-CNH induce the production of reactive oxygen species and of pro-inflammatory cytokines. The level of inflammation, although moderate, should be taken into consideration when using f-CNH for drug delivery. However, it could be exploited as an intrinsic f-CNH adjuvant function for biomedical applications requiring some activation of the immune system.
Violette Aude, Lancelot Nathalie, Poschalko Alexander, Piotto Martial, Briand Jean-Paul, Raya Jesus, Elbayed Karim, Bianco Alberto, Guichard Gilles
Exploring helical folding of oligoureas during chain elongation by high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy Journal Article
In: Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 14, no. 13, pp. 3874–3882, 2008, ISSN: 0947-6539.
Abstract | Links | BibTeX | Tags: I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Solvents, Team-Bianco, Urea
@article{violette_exploring_2008,
title = {Exploring helical folding of oligoureas during chain elongation by high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy},
author = {Aude Violette and Nathalie Lancelot and Alexander Poschalko and Martial Piotto and Jean-Paul Briand and Jesus Raya and Karim Elbayed and Alberto Bianco and Gilles Guichard},
doi = {10.1002/chem.200701923},
issn = {0947-6539},
year = {2008},
date = {2008-01-01},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
volume = {14},
number = {13},
pages = {3874--3882},
abstract = {The development of novel folding oligomers (foldamers) for biological and biomedical applications requires both precise structural information and appropriate methods to detect folding propensity. However, the synthesis and the systematic conformational investigation of large arrays of oligomers to determine the influence of factors, such as chain length, side chains, and surrounding environment, on secondary structure can be quite tedious. Herein, we show for 2.5-helical N,N'-linked oligoureas (gamma-peptide lineage) that the whole process of foldamer characterization can be accelerated by using high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy. This was achieved by monitoring a simple descriptor of conformational homogeneity (e.g., chemical shift difference between diastereotopic main chain CH2 protons) at different stages of oligourea chain growth on a solid support. HRMAS NMR experiments were conducted on two sets of oligoureas, ranging from dimer to hexamer, immobilized on DEUSS, a perdeuterated poly(oxyethylene)-based solid support swollen in solvents of low to high polarity. One evident advantage of the method is that only minute amount of material is required. In addition, the resonance of the deuterated resin is almost negligeable. On-bead NOESY spectra of high quality and with resolution comparable to that of liquid samples were obtained for longer oligomers, thus allowing detailed structural characterization.},
keywords = {I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Solvents, Team-Bianco, Urea},
pubstate = {published},
tppubtype = {article}
}
The development of novel folding oligomers (foldamers) for biological and biomedical applications requires both precise structural information and appropriate methods to detect folding propensity. However, the synthesis and the systematic conformational investigation of large arrays of oligomers to determine the influence of factors, such as chain length, side chains, and surrounding environment, on secondary structure can be quite tedious. Herein, we show for 2.5-helical N,N'-linked oligoureas (gamma-peptide lineage) that the whole process of foldamer characterization can be accelerated by using high-resolution magic-angle-spinning (HRMAS) NMR spectroscopy. This was achieved by monitoring a simple descriptor of conformational homogeneity (e.g., chemical shift difference between diastereotopic main chain CH2 protons) at different stages of oligourea chain growth on a solid support. HRMAS NMR experiments were conducted on two sets of oligoureas, ranging from dimer to hexamer, immobilized on DEUSS, a perdeuterated poly(oxyethylene)-based solid support swollen in solvents of low to high polarity. One evident advantage of the method is that only minute amount of material is required. In addition, the resonance of the deuterated resin is almost negligeable. On-bead NOESY spectra of high quality and with resolution comparable to that of liquid samples were obtained for longer oligomers, thus allowing detailed structural characterization.
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Opportunities and challenges of carbon-based nanomaterials for cancer therapy Journal Article
In: Expert Opinion on Drug Delivery, vol. 5, no. 3, pp. 331–342, 2008, ISSN: 1742-5247.
Abstract | Links | BibTeX | Tags: Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission
@article{bianco_opportunities_2008,
title = {Opportunities and challenges of carbon-based nanomaterials for cancer therapy},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1517/17425247.5.3.331},
issn = {1742-5247},
year = {2008},
date = {2008-01-01},
journal = {Expert Opinion on Drug Delivery},
volume = {5},
number = {3},
pages = {331--342},
abstract = {The possibility of incorporating carbon-based nanomaterials into living systems has opened the way for the investigation of their potential applications in the emerging field of nanomedicine. A wide variety of different nanomaterials based on allotropic forms of carbon, such as nanotubes, nanohorns and nanodiamonds, are currently being explored towards different biomedical applications. In this review, we discuss the recent advances in the development of these novel nanomaterials for cancer therapy. A comparison between the characteristics, the advantages, the drawbacks, the benefits and the risks associated with these novel biocompatible forms of carbon is presented here.},
keywords = {Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission},
pubstate = {published},
tppubtype = {article}
}
The possibility of incorporating carbon-based nanomaterials into living systems has opened the way for the investigation of their potential applications in the emerging field of nanomedicine. A wide variety of different nanomaterials based on allotropic forms of carbon, such as nanotubes, nanohorns and nanodiamonds, are currently being explored towards different biomedical applications. In this review, we discuss the recent advances in the development of these novel nanomaterials for cancer therapy. A comparison between the characteristics, the advantages, the drawbacks, the benefits and the risks associated with these novel biocompatible forms of carbon is presented here.
2007
Lacerda Lara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon nanotube cell translocation and delivery of nucleic acidsin vitro and in vivo Journal Article
In: Journal of Materials Chemistry, vol. 18, no. 1, pp. 17–22, 2007, ISSN: 1364-5501.
Abstract | Links | BibTeX | Tags: I2CT, Team-Bianco
@article{lacerda_carbon_2007,
title = {Carbon nanotube cell translocation and delivery of nucleic acidsin vitro and in vivo},
author = {Lara Lacerda and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
url = {https://pubs.rsc.org/en/content/articlelanding/2008/jm/b711554g},
doi = {10.1039/B711554G},
issn = {1364-5501},
year = {2007},
date = {2007-12-01},
urldate = {2020-03-31},
journal = {Journal of Materials Chemistry},
volume = {18},
number = {1},
pages = {17--22},
abstract = {In the last few years, the carbon nanotube (CNT) field has seen a new direction of investigation growing rapidly, along with the interest of more researchers from diverse fields of expertise interested in this new material in an attempt to exploit their properties in biomedical applications. Here we describe the most recently reported work on the application of CNT for gene encoding nucleic acid (DNA and RNA) delivery purposes by using in vitro and in vivo models. Several groups have now successfully observed the cellular internalisation of nucleic acids with the aid of CNT following very different protocols. The main processes for the internalisation pathways and intracellular release of the nucleic acids are here reviewed. Furthermore, we have just started to see some initial studies of in vivo work using siRNA-CNT conjugates to achieve silencing in tumour tissue. Admittedly, it is still very early days for the technology, but future studies are necessary, and will surely appear, in order to determine the feasibility of bringing the CNT closer to the clinic.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
In the last few years, the carbon nanotube (CNT) field has seen a new direction of investigation growing rapidly, along with the interest of more researchers from diverse fields of expertise interested in this new material in an attempt to exploit their properties in biomedical applications. Here we describe the most recently reported work on the application of CNT for gene encoding nucleic acid (DNA and RNA) delivery purposes by using in vitro and in vivo models. Several groups have now successfully observed the cellular internalisation of nucleic acids with the aid of CNT following very different protocols. The main processes for the internalisation pathways and intracellular release of the nucleic acids are here reviewed. Furthermore, we have just started to see some initial studies of in vivo work using siRNA-CNT conjugates to achieve silencing in tumour tissue. Admittedly, it is still very early days for the technology, but future studies are necessary, and will surely appear, in order to determine the feasibility of bringing the CNT closer to the clinic.
Lacerda Lara, Raffa Simona, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Cell-penetrating CNTs for delivery of therapeutics Journal Article
In: Nano Today, vol. 2, no. 6, pp. 38–43, 2007, ISSN: 1748-0132.
Abstract | Links | BibTeX | Tags: I2CT, Team-Bianco
@article{lacerda_cell-penetrating_2007,
title = {Cell-penetrating CNTs for delivery of therapeutics},
author = {Lara Lacerda and Simona Raffa and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
url = {http://www.sciencedirect.com/science/article/pii/S174801320770172X},
doi = {10.1016/S1748-0132(07)70172-X},
issn = {1748-0132},
year = {2007},
date = {2007-12-01},
urldate = {2020-03-31},
journal = {Nano Today},
volume = {2},
number = {6},
pages = {38--43},
abstract = {The use of carbon-based nanostructures, such as carbon nanotubes, in biomedicine is increasingly attracting attention. One key advantage of carbon nanotubes is their ability to translocate through plasma membranes, allowing their use for the delivery of therapeutically active molecules in a manner that resembles cell-penetrating peptides. Moreover, exploitation of their unique electrical, optical, thermal, and spectroscopic properties in a biological context is hoped to yield great advances in the detection, monitoring, and therapy of disease. Here we offer a speculative overview of the general principles behind the mechanism of carbon nanotube penetration of the plasma membrane and a snapshot of the different therapeutic modalities based on these fascinating nanostructures that are currently being investigated.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The use of carbon-based nanostructures, such as carbon nanotubes, in biomedicine is increasingly attracting attention. One key advantage of carbon nanotubes is their ability to translocate through plasma membranes, allowing their use for the delivery of therapeutically active molecules in a manner that resembles cell-penetrating peptides. Moreover, exploitation of their unique electrical, optical, thermal, and spectroscopic properties in a biological context is hoped to yield great advances in the detection, monitoring, and therapy of disease. Here we offer a speculative overview of the general principles behind the mechanism of carbon nanotube penetration of the plasma membrane and a snapshot of the different therapeutic modalities based on these fascinating nanostructures that are currently being investigated.
Habib Mohammed, Rivas Magali Noval, Chamekh Mustapha, Wieckowski Sébastien, Sun Weimin, Bianco Alberto, Trouche Nathalie, Chaloin Olivier, Dumortier Hélène, Goldman Michel, Guichard Gilles, Fournel Sylvie, Vray Bernard
In: Journal of Immunology (Baltimore, Md.: 1950), vol. 178, no. 11, pp. 6700–6704, 2007, ISSN: 0022-1767.
Abstract | Links | BibTeX | Tags: Animals, CD40 Antigens, CD40 Ligand, Cell Line, Cells, Chagas Disease, Cultured, Dumortier, I2CT, Inbred BALB C, Inbred C57BL, Interferon-gamma, Knockout, Mice, Molecular Mimicry, Parasitemia, T-Lymphocyte Subsets, Team-Bianco, Team-Dumortier, Trypanosoma cruzi
@article{habib_cutting_2007,
title = {Cutting edge: small molecule CD40 ligand mimetics promote control of parasitemia and enhance Ŧ cells producing IFN-gamma during experimental Trypanosoma cruzi infection},
author = {Mohammed Habib and Magali Noval Rivas and Mustapha Chamekh and Sébastien Wieckowski and Weimin Sun and Alberto Bianco and Nathalie Trouche and Olivier Chaloin and Hélène Dumortier and Michel Goldman and Gilles Guichard and Sylvie Fournel and Bernard Vray},
doi = {10.4049/jimmunol.178.11.6700},
issn = {0022-1767},
year = {2007},
date = {2007-06-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {178},
number = {11},
pages = {6700--6704},
abstract = {Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-gamma. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textless3 kDa) mimicking trimeric CD40L (mini CD40Ls(-1) and (-2)) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8(+) T cells, and IFN-gamma production. Mice surviving T. cruzi infection in the presence of miniCD40L(-1) were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.},
keywords = {Animals, CD40 Antigens, CD40 Ligand, Cell Line, Cells, Chagas Disease, Cultured, Dumortier, I2CT, Inbred BALB C, Inbred C57BL, Interferon-gamma, Knockout, Mice, Molecular Mimicry, Parasitemia, T-Lymphocyte Subsets, Team-Bianco, Team-Dumortier, Trypanosoma cruzi},
pubstate = {published},
tppubtype = {article}
}
Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-gamma. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textless3 kDa) mimicking trimeric CD40L (mini CD40Ls(-1) and (-2)) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8(+) T cells, and IFN-gamma production. Mice surviving T. cruzi infection in the presence of miniCD40L(-1) were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.
Habib Mohammed, Rivas Magali Noval, Chamekh Mustapha, Wieckowski Sébastien, Sun Weimin, Bianco Alberto, Trouche Nathalie, Chaloin Olivier, Dumortier Hélène, Goldman Michel, Guichard Gilles, Fournel Sylvie, Vray Bernard
In: The Journal of Immunology, vol. 178, no. 11, pp. 6700–6704, 2007, ISSN: 0022-1767, 1550-6606.
Abstract | Links | BibTeX | Tags: I2CT, Team-Bianco
@article{habib_cutting_2007b,
title = {Cutting Edge: Small Molecule CD40 Ligand Mimetics Promote Control of Parasitemia and Enhance Ŧ Cells Producing IFN-γ during Experimental Trypanosoma cruzi Infection},
author = {Mohammed Habib and Magali Noval Rivas and Mustapha Chamekh and Sébastien Wieckowski and Weimin Sun and Alberto Bianco and Nathalie Trouche and Olivier Chaloin and Hélène Dumortier and Michel Goldman and Gilles Guichard and Sylvie Fournel and Bernard Vray},
url = {https://www.jimmunol.org/content/178/11/6700},
doi = {10.4049/jimmunol.178.11.6700},
issn = {0022-1767, 1550-6606},
year = {2007},
date = {2007-06-01},
urldate = {2020-03-31},
journal = {The Journal of Immunology},
volume = {178},
number = {11},
pages = {6700--6704},
abstract = {Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-γ. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textbackslashtextless3 kDa) mimicking trimeric CD40L (mini CD40Ls-1 and -2) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8+ T cells, and IFN-γ production. Mice surviving T. cruzi infection in the presence of miniCD40L-1 were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Host resistance to Trypanosoma cruzi infection depends on a type 1 response characterized by a strong production of IL-12 and IFN-γ. Amplifying this response through CD40 triggering results in control of parasitemia. Two newly synthesized molecules (textbackslashtextless3 kDa) mimicking trimeric CD40L (mini CD40Ls-1 and -2) bind to CD40, activate murine dendritic cells, and elicit IL-12 production. Wild-type but not CD40 knockout mice exhibited a sharp decrease of parasitemia and mortality when inoculated with T. cruzi mixed with miniCD40Ls. Moreover, the immunosuppression induced by T. cruzi infection was impaired in mice treated with miniCD40Ls, as shown by proliferation of splenic lymphocytes, percentage of CD8+ T cells, and IFN-γ production. Mice surviving T. cruzi infection in the presence of miniCD40L-1 were immunized against a challenge infection. Our results indicate that CD40L mimetics are effective in vivo and promote the control of T. cruzi infection by overcoming the immunosuppression usually induced by the parasites.
Kostarelos Kostas, Lacerda Lara, Pastorin Giorgia, Wu Wei, Wieckowski Sébastien, Luangsivilay Jacqueline, Godefroy Sylvie, Pantarotto Davide, Briand Jean-Paul, Muller Sylviane, Prato Maurizio, Bianco Alberto
Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type Journal Article
In: Nature Nanotechnology, vol. 2, no. 2, pp. 108–113, 2007, ISSN: 1748-3395.
Links | BibTeX | Tags: Animals, carbon, Cell Membrane, Cells, Cultured, Diffusion, Humans, I2CT, Nanotubes, Team-Bianco
@article{kostarelos_cellular_2007,
title = {Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type},
author = {Kostas Kostarelos and Lara Lacerda and Giorgia Pastorin and Wei Wu and Sébastien Wieckowski and Jacqueline Luangsivilay and Sylvie Godefroy and Davide Pantarotto and Jean-Paul Briand and Sylviane Muller and Maurizio Prato and Alberto Bianco},
doi = {10.1038/nnano.2006.209},
issn = {1748-3395},
year = {2007},
date = {2007-02-01},
journal = {Nature Nanotechnology},
volume = {2},
number = {2},
pages = {108--113},
keywords = {Animals, carbon, Cell Membrane, Cells, Cultured, Diffusion, Humans, I2CT, Nanotubes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Monneaux Fanny, Muller Sylviane
Peptide-based therapy in lupus: promising data Journal Article
In: Advances in Experimental Medicine and Biology, vol. 601, pp. 105–112, 2007, ISSN: 0065-2598.
Abstract | Links | BibTeX | Tags: Adrenal Cortex Hormones, Animals, Autoantigens, Autoimmune Diseases, Cyclophosphamide, Epitopes, Humans, I2CT, Immune System, Immunosuppressive Agents, inflammation, Lupus Erythematosus, Monneaux, Peptides, Systemic, T-Lymphocytes, Team-Dumortier
@article{monneaux_peptide-based_2007,
title = {Peptide-based therapy in lupus: promising data},
author = {Fanny Monneaux and Sylviane Muller},
doi = {10.1007/978-0-387-72005-0_11},
issn = {0065-2598},
year = {2007},
date = {2007-01-01},
journal = {Advances in Experimental Medicine and Biology},
volume = {601},
pages = {105--112},
abstract = {Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of multifactorial aetiology, characterized by inflammation and damage of various tissues and organs. Current treatments of the disease are mainly based on immunosuppressive drugs such as corticosteroids and cyclophosphamide. Although these treatments have reduced mortality and morbidity, they cause a non-specific immune suppression. To avoid these side effects, our efforts should focus on the development of alternative therapeutic strategies, which consist, for example in specific T cell targeting using autoantigen-derived peptides identified as sequences encompassing major epitopes.},
keywords = {Adrenal Cortex Hormones, Animals, Autoantigens, Autoimmune Diseases, Cyclophosphamide, Epitopes, Humans, I2CT, Immune System, Immunosuppressive Agents, inflammation, Lupus Erythematosus, Monneaux, Peptides, Systemic, T-Lymphocytes, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of multifactorial aetiology, characterized by inflammation and damage of various tissues and organs. Current treatments of the disease are mainly based on immunosuppressive drugs such as corticosteroids and cyclophosphamide. Although these treatments have reduced mortality and morbidity, they cause a non-specific immune suppression. To avoid these side effects, our efforts should focus on the development of alternative therapeutic strategies, which consist, for example in specific T cell targeting using autoantigen-derived peptides identified as sequences encompassing major epitopes.
Parietti Véronique, Chifflot Hélène, Muller Sylviane, Monneaux Fanny
Regulatory Ŧ cells and systemic lupus erythematosus Journal Article
In: Annals of the New York Academy of Sciences, vol. 1108, pp. 64–75, 2007, ISSN: 0077-8923.
Abstract | Links | BibTeX | Tags: Animals, Autoimmunity, Humans, I2CT, Immune Tolerance, Lupus Erythematosus, Monneaux, Regulatory, Systemic, T-Lymphocyte Subsets, T-Lymphocytes, Team-Dumortier
@article{parietti_regulatory_2007,
title = {Regulatory Ŧ cells and systemic lupus erythematosus},
author = {Véronique Parietti and Hélène Chifflot and Sylviane Muller and Fanny Monneaux},
doi = {10.1196/annals.1422.007},
issn = {0077-8923},
year = {2007},
date = {2007-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1108},
pages = {64--75},
abstract = {Regulatory T cells, especially CD4+CD25+ T cells, "natural killer" T cells and gammadelta T cells, are central in the maintenance of peripheral tolerance and the protection from the development of autoimmune diseases. Numerical or functional modifications of these cell populations were demonstrated to lead to the breakdown of tolerance and the emergence of autoimmunity. Involvement of regulatory T cells in the pathogenesis of systemic autoimmune diseases, such as systemic lupus erythematosus, might be of first importance. In murine models and patients with lupus, these regulatory T cells seem to be reduced in number. Functional deficiencies have also been described in a few studies. A better knowledge of regulatory T cell functional properties in systemic autoimmune diseases is essential to manipulate these cells and hopefully to restore immune tolerance.},
keywords = {Animals, Autoimmunity, Humans, I2CT, Immune Tolerance, Lupus Erythematosus, Monneaux, Regulatory, Systemic, T-Lymphocyte Subsets, T-Lymphocytes, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Regulatory T cells, especially CD4+CD25+ T cells, "natural killer" T cells and gammadelta T cells, are central in the maintenance of peripheral tolerance and the protection from the development of autoimmune diseases. Numerical or functional modifications of these cell populations were demonstrated to lead to the breakdown of tolerance and the emergence of autoimmunity. Involvement of regulatory T cells in the pathogenesis of systemic autoimmune diseases, such as systemic lupus erythematosus, might be of first importance. In murine models and patients with lupus, these regulatory T cells seem to be reduced in number. Functional deficiencies have also been described in a few studies. A better knowledge of regulatory T cell functional properties in systemic autoimmune diseases is essential to manipulate these cells and hopefully to restore immune tolerance.
Monneaux F, Muller S
[The spliceosome and its interest for lupus therapy] Journal Article
In: La Revue De Medecine Interne, vol. 28, no. 10, pp. 725–728, 2007, ISSN: 0248-8663.
Abstract | Links | BibTeX | Tags: Amino Acid Motifs, Animals, Antibodies, CD4-Positive T-Lymphocytes, Conserved Sequence, DNA, Epitopes, Haplotypes, Humans, I2CT, Immune Tolerance, Inbred MRL lpr, Inbred NZB, Lupus Erythematosus, Mice, Monneaux, Phosphoserine, Protein, Recombinant, Ribonucleoprotein, Sequence Analysis, Serine, Spliceosomes, Systemic, Team-Dumortier, U1 Small Nuclear
@article{monneaux_spliceosome_2007,
title = {[The spliceosome and its interest for lupus therapy]},
author = {F Monneaux and S Muller},
doi = {10.1016/j.revmed.2007.05.003},
issn = {0248-8663},
year = {2007},
date = {2007-01-01},
journal = {La Revue De Medecine Interne},
volume = {28},
number = {10},
pages = {725--728},
abstract = {INTRODUCTION: The spliceosome, which is a particle containing a molecule of U-RNA and proteins that are specific to each U ribonuclear particle (U-snRNP) or common to every U-snRNPs, is one of the numerous nuclear targets recognized by the antibodies (Abs) and CD4+ T cells from patients with systemic lupus erythematosus and lupus mice.
EXEGESIS: We recently characterized a peptide from the spliceosomal protein U1-70K (sequence 131-151), which is recognized by the Abs and CD4+ T cells from lupus mice and patients. This peptide contains a conserved RNP1 motif, which is also present in other spliceosomal proteins targeted by the Abs from individuals with lupus. We further showed that peptide 131-151 containing a phosphoserine at position 140 (peptide P140) possessed tolerogenic properties in lupus mice and was recognized by the Abs and CD4+ T cells from lupus patients.
CONCLUSION: Thanks to its RNP1 motif, the peptide P140 might play an important role in the initiation and perpetuation steps of the humoral and cellular immune response diversification in lupus individuals. Therapeutic and particularly immunomodulating properties of P140 peptide are being evaluated in humans (a phase III clinical trial will be undertaken in the next weeks).},
keywords = {Amino Acid Motifs, Animals, Antibodies, CD4-Positive T-Lymphocytes, Conserved Sequence, DNA, Epitopes, Haplotypes, Humans, I2CT, Immune Tolerance, Inbred MRL lpr, Inbred NZB, Lupus Erythematosus, Mice, Monneaux, Phosphoserine, Protein, Recombinant, Ribonucleoprotein, Sequence Analysis, Serine, Spliceosomes, Systemic, Team-Dumortier, U1 Small Nuclear},
pubstate = {published},
tppubtype = {article}
}
INTRODUCTION: The spliceosome, which is a particle containing a molecule of U-RNA and proteins that are specific to each U ribonuclear particle (U-snRNP) or common to every U-snRNPs, is one of the numerous nuclear targets recognized by the antibodies (Abs) and CD4+ T cells from patients with systemic lupus erythematosus and lupus mice.
EXEGESIS: We recently characterized a peptide from the spliceosomal protein U1-70K (sequence 131-151), which is recognized by the Abs and CD4+ T cells from lupus mice and patients. This peptide contains a conserved RNP1 motif, which is also present in other spliceosomal proteins targeted by the Abs from individuals with lupus. We further showed that peptide 131-151 containing a phosphoserine at position 140 (peptide P140) possessed tolerogenic properties in lupus mice and was recognized by the Abs and CD4+ T cells from lupus patients.
CONCLUSION: Thanks to its RNP1 motif, the peptide P140 might play an important role in the initiation and perpetuation steps of the humoral and cellular immune response diversification in lupus individuals. Therapeutic and particularly immunomodulating properties of P140 peptide are being evaluated in humans (a phase III clinical trial will be undertaken in the next weeks).
EXEGESIS: We recently characterized a peptide from the spliceosomal protein U1-70K (sequence 131-151), which is recognized by the Abs and CD4+ T cells from lupus mice and patients. This peptide contains a conserved RNP1 motif, which is also present in other spliceosomal proteins targeted by the Abs from individuals with lupus. We further showed that peptide 131-151 containing a phosphoserine at position 140 (peptide P140) possessed tolerogenic properties in lupus mice and was recognized by the Abs and CD4+ T cells from lupus patients.
CONCLUSION: Thanks to its RNP1 motif, the peptide P140 might play an important role in the initiation and perpetuation steps of the humoral and cellular immune response diversification in lupus individuals. Therapeutic and particularly immunomodulating properties of P140 peptide are being evaluated in humans (a phase III clinical trial will be undertaken in the next weeks).
Woods Anne, Monneaux Fanny, Soulas-Sprauel Pauline, Muller Sylviane, Martin Thierry, Korganow Anne-Sophie, Pasquali Jean-Louis
Influenza virus-induced type I interferon leads to polyclonal B-cell activation but does not break down B-cell tolerance Journal Article
In: Journal of Virology, vol. 81, no. 22, pp. 12525–12534, 2007, ISSN: 0022-538X.
Abstract | Links | BibTeX | Tags: Animals, Antibody Formation, Autoantibodies, Autoantigens, Autoimmunity, B-Lymphocytes, Humans, I2CT, Immune Tolerance, Immunoglobulin M, Inbred Strains, Influenza A virus, Interferon Type I, Lymphocyte Activation, Mice, Monneaux, Rheumatoid Factor, Team-Dumortier, transgenic
@article{woods_influenza_2007,
title = {Influenza virus-induced type I interferon leads to polyclonal B-cell activation but does not break down B-cell tolerance},
author = {Anne Woods and Fanny Monneaux and Pauline Soulas-Sprauel and Sylviane Muller and Thierry Martin and Anne-Sophie Korganow and Jean-Louis Pasquali},
doi = {10.1128/JVI.00839-07},
issn = {0022-538X},
year = {2007},
date = {2007-01-01},
journal = {Journal of Virology},
volume = {81},
number = {22},
pages = {12525--12534},
abstract = {The link between infection and autoimmunity is not yet well understood. This study was designed to evaluate if an acute viral infection known to induce type I interferon production, like influenza, can by itself be responsible for the breakdown of immune tolerance and for autoimmunity. We first tested the effects of influenza virus on B cells in vitro. We then infected different transgenic mice expressing human rheumatoid factors (RF) in the absence or in the constitutive presence of the autoantigen (human immunoglobulin G [IgG]) and young lupus-prone mice [(NZB x NZW)F(1)] with influenza virus and looked for B-cell activation. In vitro, the virus induces B-cell activation through type I interferon production by non-B cells but does not directly stimulate purified B cells. In vivo, both RF and non-RF B cells were activated in an autoantigen-independent manner. This activation was abortive since IgM and IgM-RF production levels were not increased in infected mice compared to uninfected controls, whether or not anti-influenza virus human IgG was detected and even after viral rechallenge. As in RF transgenic mice, acute viral infection of (NZB x NZW)F(1) mice induced only an abortive activation of B cells and no increase in autoantibody production compared to uninfected animals. Taken together, these experiments show that virus-induced acute type I interferon production is not able by itself to break down B-cell tolerance in both normal and autoimmune genetic backgrounds.},
keywords = {Animals, Antibody Formation, Autoantibodies, Autoantigens, Autoimmunity, B-Lymphocytes, Humans, I2CT, Immune Tolerance, Immunoglobulin M, Inbred Strains, Influenza A virus, Interferon Type I, Lymphocyte Activation, Mice, Monneaux, Rheumatoid Factor, Team-Dumortier, transgenic},
pubstate = {published},
tppubtype = {article}
}
The link between infection and autoimmunity is not yet well understood. This study was designed to evaluate if an acute viral infection known to induce type I interferon production, like influenza, can by itself be responsible for the breakdown of immune tolerance and for autoimmunity. We first tested the effects of influenza virus on B cells in vitro. We then infected different transgenic mice expressing human rheumatoid factors (RF) in the absence or in the constitutive presence of the autoantigen (human immunoglobulin G [IgG]) and young lupus-prone mice [(NZB x NZW)F(1)] with influenza virus and looked for B-cell activation. In vitro, the virus induces B-cell activation through type I interferon production by non-B cells but does not directly stimulate purified B cells. In vivo, both RF and non-RF B cells were activated in an autoantigen-independent manner. This activation was abortive since IgM and IgM-RF production levels were not increased in infected mice compared to uninfected controls, whether or not anti-influenza virus human IgG was detected and even after viral rechallenge. As in RF transgenic mice, acute viral infection of (NZB x NZW)F(1) mice induced only an abortive activation of B cells and no increase in autoantibody production compared to uninfected animals. Taken together, these experiments show that virus-induced acute type I interferon production is not able by itself to break down B-cell tolerance in both normal and autoimmune genetic backgrounds.
Monneaux Fanny, Parietti Véronique, Briand Jean-Paul, Muller Sylviane
Importance of spliceosomal RNP1 motif for intermolecular Ŧ-B cell spreading and tolerance restoration in lupus Journal Article
In: Arthritis Research & Therapy, vol. 9, no. 5, pp. R111, 2007, ISSN: 1478-6362.
Abstract | Links | BibTeX | Tags: Amino Acid Motifs, Amino Acid Sequence, Animals, B-Lymphocytes, I2CT, Immune Tolerance, Inbred MRL lpr, Lupus Erythematosus, Mice, Molecular Sequence Data, Monneaux, Ribonucleoproteins, RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Spliceosomes, Systemic, T-Lymphocytes, Team-Dumortier
@article{monneaux_importance_2007,
title = {Importance of spliceosomal RNP1 motif for intermolecular Ŧ-B cell spreading and tolerance restoration in lupus},
author = {Fanny Monneaux and Véronique Parietti and Jean-Paul Briand and Sylviane Muller},
doi = {10.1186/ar2317},
issn = {1478-6362},
year = {2007},
date = {2007-01-01},
journal = {Arthritis Research & Therapy},
volume = {9},
number = {5},
pages = {R111},
abstract = {We previously demonstrated the importance of the RNP1 motif-bearing region 131-151 of the U1-70K spliceosomal protein in the intramolecular T-B spreading that occurs in MRL/lpr lupus mice. Here, we analyze the involvement of RNP1 motif in the development and prevention of naturally-occurring intermolecular T-B cell diversification. We found that MRL/lpr peripheral blood lymphocytes proliferated in response to peptides containing or corresponding exactly to the RNP1 motif of spliceosomal U1-70K, U1-A and hnRNP-A2 proteins. We also demonstrated that rabbit antibodies to peptide 131-151 cross-reacted with U1-70K, U1-A and hnRNP-A2 RNP1-peptides. These antibodies recognized the U1-70K and U1-A proteins, and also U1-C and SmD1 proteins, which are devoid of RNP1 motif. Repeated administration of phosphorylated peptide P140 into MRL/lpr mice abolished T-cell response to several peptides from the U1-70K, U1-A and SmD1 proteins without affecting antibody and T-cell responses to foreign (viral) antigen in treated mice challenged with infectious virus. These results emphasized the importance of the dominant RNP1 region, which seems to be central in the activation cascade of B and T cells reacting with spliceosomal RNP1+ and RNP1- spliceosomal proteins. The tolerogenic peptide P140, which is recognized by lupus patients' CD4+ T cells and known to protect MRL/lpr mice, is able to thwart emergence of intermolecular T-cell spreading in treated animals.},
keywords = {Amino Acid Motifs, Amino Acid Sequence, Animals, B-Lymphocytes, I2CT, Immune Tolerance, Inbred MRL lpr, Lupus Erythematosus, Mice, Molecular Sequence Data, Monneaux, Ribonucleoproteins, RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Spliceosomes, Systemic, T-Lymphocytes, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
We previously demonstrated the importance of the RNP1 motif-bearing region 131-151 of the U1-70K spliceosomal protein in the intramolecular T-B spreading that occurs in MRL/lpr lupus mice. Here, we analyze the involvement of RNP1 motif in the development and prevention of naturally-occurring intermolecular T-B cell diversification. We found that MRL/lpr peripheral blood lymphocytes proliferated in response to peptides containing or corresponding exactly to the RNP1 motif of spliceosomal U1-70K, U1-A and hnRNP-A2 proteins. We also demonstrated that rabbit antibodies to peptide 131-151 cross-reacted with U1-70K, U1-A and hnRNP-A2 RNP1-peptides. These antibodies recognized the U1-70K and U1-A proteins, and also U1-C and SmD1 proteins, which are devoid of RNP1 motif. Repeated administration of phosphorylated peptide P140 into MRL/lpr mice abolished T-cell response to several peptides from the U1-70K, U1-A and SmD1 proteins without affecting antibody and T-cell responses to foreign (viral) antigen in treated mice challenged with infectious virus. These results emphasized the importance of the dominant RNP1 region, which seems to be central in the activation cascade of B and T cells reacting with spliceosomal RNP1+ and RNP1- spliceosomal proteins. The tolerogenic peptide P140, which is recognized by lupus patients' CD4+ T cells and known to protect MRL/lpr mice, is able to thwart emergence of intermolecular T-cell spreading in treated animals.
Lacerda L, Pastorin G, Gathercole D, Buddle J, Prato M, Bianco A, Kostarelos K
Intracellular Trafficking of Carbon Nanotubes by Confocal Laser Scanning Microscopy Journal Article
In: Advanced Materials, vol. 19, no. 14, pp. 1789–1789, 2007, ISSN: 1521-4095.
Links | BibTeX | Tags: Biomedical materials, Cells, Drug delivery, fluorescence, I2CT, Nanotubes, single-walled, Team-Bianco
@article{lacerda_intracellular_2007,
title = {Intracellular Trafficking of Carbon Nanotubes by Confocal Laser Scanning Microscopy},
author = {L Lacerda and G Pastorin and D Gathercole and J Buddle and M Prato and A Bianco and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200790051},
doi = {10.1002/adma.200790051},
issn = {1521-4095},
year = {2007},
date = {2007-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {19},
number = {14},
pages = {1789--1789},
keywords = {Biomedical materials, Cells, Drug delivery, fluorescence, I2CT, Nanotubes, single-walled, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Klumpp Cédric, Lacerda Lara, Chaloin Olivier, Ros Tatiana Da, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Multifunctionalised cationic fullerene adducts for gene transfer: design, synthesis and DNA complexation Journal Article
In: Chemical Communications (Cambridge, England), no. 36, pp. 3762–3764, 2007, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: DNA, Electrophoresis, Fullerenes, Gene Transfer Techniques, I2CT, Molecular Structure, Plasmids, Team-Bianco
@article{klumpp_multifunctionalised_2007,
title = {Multifunctionalised cationic fullerene adducts for gene transfer: design, synthesis and DNA complexation},
author = {Cédric Klumpp and Lara Lacerda and Olivier Chaloin and Tatiana Da Ros and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b708435h},
issn = {1359-7345},
year = {2007},
date = {2007-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {36},
pages = {3762--3764},
abstract = {Cationic poly-N,N-dimethylfulleropyrrolidinium derivatives have been designed and synthesised to complex plasmid DNA for gene delivery.},
keywords = {DNA, Electrophoresis, Fullerenes, Gene Transfer Techniques, I2CT, Molecular Structure, Plasmids, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Cationic poly-N,N-dimethylfulleropyrrolidinium derivatives have been designed and synthesised to complex plasmid DNA for gene delivery.
2006
Lacerda Lara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon nanotubes as nanomedicines: from toxicology to pharmacology Journal Article
In: Advanced Drug Delivery Reviews, vol. 58, no. 14, pp. 1460–1470, 2006, ISSN: 0169-409X.
Abstract | Links | BibTeX | Tags: Animals, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Pharmacokinetics, Pharmacology, Team-Bianco
@article{lacerda_carbon_2006,
title = {Carbon nanotubes as nanomedicines: from toxicology to pharmacology},
author = {Lara Lacerda and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1016/j.addr.2006.09.015},
issn = {0169-409X},
year = {2006},
date = {2006-12-01},
journal = {Advanced Drug Delivery Reviews},
volume = {58},
number = {14},
pages = {1460--1470},
abstract = {Various biomedical applications of carbon nanotubes have been proposed in the last few years leading to the emergence of a new field in diagnostics and therapeutics. Most of these applications will involve the administration or implantation of carbon nanotubes and their matrices into patients. The toxicological and pharmacological profile of such carbon nanotube systems developed as nanomedicines will have to be determined prior to any clinical studies undertaken. This review brings together all the toxicological and pharmacological in vivo studies that have been carried out using carbon nanotubes, to offer the first summary of the state-of-the-art in the pharmaceutical development of carbon nanotubes on the road to becoming viable and effective nanomedicines.},
keywords = {Animals, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Pharmacokinetics, Pharmacology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Various biomedical applications of carbon nanotubes have been proposed in the last few years leading to the emergence of a new field in diagnostics and therapeutics. Most of these applications will involve the administration or implantation of carbon nanotubes and their matrices into patients. The toxicological and pharmacological profile of such carbon nanotube systems developed as nanomedicines will have to be determined prior to any clinical studies undertaken. This review brings together all the toxicological and pharmacological in vivo studies that have been carried out using carbon nanotubes, to offer the first summary of the state-of-the-art in the pharmaceutical development of carbon nanotubes on the road to becoming viable and effective nanomedicines.
Hauquier Fanny, Pastorin Giorgia, Hapiot Philippe, Prato Maurizio, Bianco Alberto, Fabre Bruno
Carbon nanotube-functionalized silicon surfaces with efficient redox communication Journal Article
In: Chemical Communications (Cambridge, England), no. 43, pp. 4536–4538, 2006, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: carbon, I2CT, Nanotubes, Oxidation-Reduction, Silicon, Surface Properties, Team-Bianco
@article{hauquier_carbon_2006,
title = {Carbon nanotube-functionalized silicon surfaces with efficient redox communication},
author = {Fanny Hauquier and Giorgia Pastorin and Philippe Hapiot and Maurizio Prato and Alberto Bianco and Bruno Fabre},
doi = {10.1039/b610559a},
issn = {1359-7345},
year = {2006},
date = {2006-11-01},
journal = {Chemical Communications (Cambridge, England)},
number = {43},
pages = {4536--4538},
abstract = {Sidewall functionalized multi-walled carbon nanotubes can be covalently bound parallel to a silicon surface via a self-assembled acid-terminated monolayer used as an organic molecular glue.},
keywords = {carbon, I2CT, Nanotubes, Oxidation-Reduction, Silicon, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Sidewall functionalized multi-walled carbon nanotubes can be covalently bound parallel to a silicon surface via a self-assembled acid-terminated monolayer used as an organic molecular glue.
Dumortier Hélène, Lacotte Stéphanie, Pastorin Giorgia, Marega Riccardo, Wu Wei, Bonifazi Davide, Briand Jean-Paul, Prato Maurizio, Muller Sylviane, Bianco Alberto
Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells Journal Article
In: Nano Letters, vol. 6, no. 7, pp. 1522–1528, 2006, ISSN: 1530-6984.
Abstract | Links | BibTeX | Tags: Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, Dumortier, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco, Team-Dumortier
@article{dumortier_functionalized_2006,
title = {Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells},
author = {Hélène Dumortier and Stéphanie Lacotte and Giorgia Pastorin and Riccardo Marega and Wei Wu and Davide Bonifazi and Jean-Paul Briand and Maurizio Prato and Sylviane Muller and Alberto Bianco},
doi = {10.1021/nl061160x},
issn = {1530-6984},
year = {2006},
date = {2006-07-01},
journal = {Nano Letters},
volume = {6},
number = {7},
pages = {1522--1528},
abstract = {Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.},
keywords = {Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, Dumortier, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.
Dumortier Hélène, Lacotte Stéphanie, Pastorin Giorgia, Marega Riccardo, Wu Wei, Bonifazi Davide, Briand Jean-Paul, Prato Maurizio, Muller Sylviane, Bianco Alberto
Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells Journal Article
In: Nano Letters, vol. 6, no. 7, pp. 1522–1528, 2006, ISSN: 1530-6984.
Abstract | Links | BibTeX | Tags: Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco
@article{dumortier_functionalized_2006b,
title = {Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells},
author = {Hélène Dumortier and Stéphanie Lacotte and Giorgia Pastorin and Riccardo Marega and Wei Wu and Davide Bonifazi and Jean-Paul Briand and Maurizio Prato and Sylviane Muller and Alberto Bianco},
doi = {10.1021/nl061160x},
issn = {1530-6984},
year = {2006},
date = {2006-07-01},
journal = {Nano Letters},
volume = {6},
number = {7},
pages = {1522--1528},
abstract = {Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.},
keywords = {Amides, B-Lymphocytes, Biotechnology, carbon, Cell Survival, Cytokines, I2CT, Macrophages, Molecular Structure, Nanotubes, Oxidation-Reduction, T-Lymphocytes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction (f-CNTs 1 and 2) and the oxidation/amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.
Pastorin Giorgia, Marchesan Silvia, Hoebeke Johan, Ros Tatiana Da, Ehret-Sabatier Laurence, Briand Jean-Paul, Prato Maurizio, Bianco Alberto
Design and activity of cationic fullerene derivatives as inhibitors of acetylcholinesterase Journal Article
In: Organic & Biomolecular Chemistry, vol. 4, no. 13, pp. 2556–2562, 2006, ISSN: 1477-0520.
Abstract | Links | BibTeX | Tags: Acetylcholinesterase, Binding Sites, Cations, Cholinesterase Inhibitors, Drug Design, Fullerenes, I2CT, Models, Molecular, Team-Bianco
@article{pastorin_design_2006,
title = {Design and activity of cationic fullerene derivatives as inhibitors of acetylcholinesterase},
author = {Giorgia Pastorin and Silvia Marchesan and Johan Hoebeke and Tatiana Da Ros and Laurence Ehret-Sabatier and Jean-Paul Briand and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b604361e},
issn = {1477-0520},
year = {2006},
date = {2006-07-01},
journal = {Organic & Biomolecular Chemistry},
volume = {4},
number = {13},
pages = {2556--2562},
abstract = {Four different regioisomers of cationic bis-N,N-dimethylfulleropyrrolidinium salts have been prepared and evaluated as inhibitors of the enzymatic activity of acetylcholinesterase. These fullerene-based derivatives were found to be noncompetitive inhibitors of acetylthiocholine hydrolysis. Molecular modelling was used to describe the possible interactions between the fullerene cage and the amino acids surrounding the cavity of the enzyme. The cationic C(60) derivatives used in this study represent a new class of molecules potentially able to modulate the enzymatic activity of acetylcholinesterase.},
keywords = {Acetylcholinesterase, Binding Sites, Cations, Cholinesterase Inhibitors, Drug Design, Fullerenes, I2CT, Models, Molecular, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Four different regioisomers of cationic bis-N,N-dimethylfulleropyrrolidinium salts have been prepared and evaluated as inhibitors of the enzymatic activity of acetylcholinesterase. These fullerene-based derivatives were found to be noncompetitive inhibitors of acetylthiocholine hydrolysis. Molecular modelling was used to describe the possible interactions between the fullerene cage and the amino acids surrounding the cavity of the enzyme. The cationic C(60) derivatives used in this study represent a new class of molecules potentially able to modulate the enzymatic activity of acetylcholinesterase.
Bianco Alberto, Maggini Michele, Nogarole Marco, Scorrano Gianfranco
Hydrolysis Rate of Functionalized Fullerenes Bearing Alkoxysilanes: A Comparative Study Journal Article
In: European Journal of Organic Chemistry, vol. 2006, no. 13, pp. 2934–2941, 2006, ISSN: 1434-193X.
Abstract | Links | BibTeX | Tags: Alkoxysilanes, Fullerenes, Fulleropyrrolidines, I2CT, Sol–gel, Team-Bianco
@article{bianco_hydrolysis_2006,
title = {Hydrolysis Rate of Functionalized Fullerenes Bearing Alkoxysilanes: A Comparative Study},
author = {Alberto Bianco and Michele Maggini and Marco Nogarole and Gianfranco Scorrano},
url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/ejoc.200600084},
doi = {10.1002/ejoc.200600084},
issn = {1434-193X},
year = {2006},
date = {2006-07-01},
urldate = {2020-03-31},
journal = {European Journal of Organic Chemistry},
volume = {2006},
number = {13},
pages = {2934--2941},
abstract = {Abstract Soluble fulleropyrrolidines bearing a trialkoxysilyl functionality (methoxy, ethoxy, butoxy, and isopropoxy) have been prepared and characterized. The hydrolysis rate constant for each fulleropyrrolidine was measured with 1H NMR spectroscopy by following the disappearance of selected resonances of the fullerene substrate under the conditions (HCl/H2O/THF) used for the preparation of fullerene-doped sol?gel glasses. It has been found that fulleropyrrolidine 1, bearing the trimethoxysilyl group, hydrolyzes faster than substrates 2?7 and should be the reagent of choice to minimize aggregation of the fullerene spheroid in sol?gel glassy matrices. The triethoxysilyl derivative 2, our benchmark fulleropyrrolidine for incorporation in sol?gel glasses, has the secondfastest hydrolysis rate. (? Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)},
keywords = {Alkoxysilanes, Fullerenes, Fulleropyrrolidines, I2CT, Sol–gel, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Abstract Soluble fulleropyrrolidines bearing a trialkoxysilyl functionality (methoxy, ethoxy, butoxy, and isopropoxy) have been prepared and characterized. The hydrolysis rate constant for each fulleropyrrolidine was measured with 1H NMR spectroscopy by following the disappearance of selected resonances of the fullerene substrate under the conditions (HCl/H2O/THF) used for the preparation of fullerene-doped sol?gel glasses. It has been found that fulleropyrrolidine 1, bearing the trimethoxysilyl group, hydrolyzes faster than substrates 2?7 and should be the reagent of choice to minimize aggregation of the fullerene spheroid in sol?gel glassy matrices. The triethoxysilyl derivative 2, our benchmark fulleropyrrolidine for incorporation in sol?gel glasses, has the secondfastest hydrolysis rate. (? Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Bianco Alberto, Fournel Sylvie, Wieckowski Sébastien, Hoebeke Johan, Guichard Gilles
Solid-phase synthesis of CD40L mimetics Journal Article
In: Organic & Biomolecular Chemistry, vol. 4, no. 8, pp. 1461–1463, 2006, ISSN: 1477-0520.
Abstract | Links | BibTeX | Tags: Apoptosis, CD40 Ligand, Cell Line, Chromatography, Combinatorial Chemistry Techniques, High Pressure Liquid, Humans, I2CT, Molecular Mimicry, Molecular Structure, Protein Binding, Team-Bianco, tumor
@article{bianco_solid-phase_2006,
title = {Solid-phase synthesis of CD40L mimetics},
author = {Alberto Bianco and Sylvie Fournel and Sébastien Wieckowski and Johan Hoebeke and Gilles Guichard},
doi = {10.1039/b601528j},
issn = {1477-0520},
year = {2006},
date = {2006-04-01},
journal = {Organic & Biomolecular Chemistry},
volume = {4},
number = {8},
pages = {1461--1463},
abstract = {The C3-symmetric molecule has been previously shown to mimic CD40 ligand (CD40L) homotrimers and to display effector functions. This molecule consists of a cyclic hexapeptide core containing the repetition of the D-Ala-L-Lys motif. The side chains of the lysine residues have been modified by appending the CD40L-derived sequence 143Lys-Gly-Tyr-Tyr146 via a 6-aminohexanoic acid residue as a spacer. The present report describes a general solid-phase synthesis approach to and related trimeric architectures. In addition, their CD40 binding properties as well as their effector functions have been evaluated.},
keywords = {Apoptosis, CD40 Ligand, Cell Line, Chromatography, Combinatorial Chemistry Techniques, High Pressure Liquid, Humans, I2CT, Molecular Mimicry, Molecular Structure, Protein Binding, Team-Bianco, tumor},
pubstate = {published},
tppubtype = {article}
}
The C3-symmetric molecule has been previously shown to mimic CD40 ligand (CD40L) homotrimers and to display effector functions. This molecule consists of a cyclic hexapeptide core containing the repetition of the D-Ala-L-Lys motif. The side chains of the lysine residues have been modified by appending the CD40L-derived sequence 143Lys-Gly-Tyr-Tyr146 via a 6-aminohexanoic acid residue as a spacer. The present report describes a general solid-phase synthesis approach to and related trimeric architectures. In addition, their CD40 binding properties as well as their effector functions have been evaluated.
Tasis Dimitrios, Tagmatarchis Nikos, Bianco Alberto, Prato Maurizio
Chemistry of carbon nanotubes Journal Article
In: Chemical Reviews, vol. 106, no. 3, pp. 1105–1136, 2006, ISSN: 0009-2665.
Links | BibTeX | Tags: Animals, Biopolymers, carbon, Chemical Phenomena, Chemistry, I2CT, Molecular Structure, Nanotubes, Solubility, Surface Properties, Team-Bianco
@article{tasis_chemistry_2006,
title = {Chemistry of carbon nanotubes},
author = {Dimitrios Tasis and Nikos Tagmatarchis and Alberto Bianco and Maurizio Prato},
doi = {10.1021/cr050569o},
issn = {0009-2665},
year = {2006},
date = {2006-03-01},
journal = {Chemical Reviews},
volume = {106},
number = {3},
pages = {1105--1136},
keywords = {Animals, Biopolymers, carbon, Chemical Phenomena, Chemistry, I2CT, Molecular Structure, Nanotubes, Solubility, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Pastorin Giorgia, Wu Wei, Wieckowski Sébastien, Briand Jean-Paul, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Double functionalization of carbon nanotubes for multimodal drug delivery Journal Article
In: Chemical Communications (Cambridge, England), no. 11, pp. 1182–1184, 2006, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: Azo Compounds, carbon, Cyclization, Drug Carriers, I2CT, Microscopy, Nanotubes, Pharmaceutical Preparations, Scanning Tunneling, Solubility, Team-Bianco
@article{pastorin_double_2006,
title = {Double functionalization of carbon nanotubes for multimodal drug delivery},
author = {Giorgia Pastorin and Wei Wu and Sébastien Wieckowski and Jean-Paul Briand and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b516309a},
issn = {1359-7345},
year = {2006},
date = {2006-03-01},
journal = {Chemical Communications (Cambridge, England)},
number = {11},
pages = {1182--1184},
abstract = {Multi-walled carbon nanotubes have been covalently functionalized via 1,3-dipolar cycloaddition of azomethine ylides with orthogonally protected amino functions that can be selectively deprotected and subsequently modified with drugs and fluorescent probes.},
keywords = {Azo Compounds, carbon, Cyclization, Drug Carriers, I2CT, Microscopy, Nanotubes, Pharmaceutical Preparations, Scanning Tunneling, Solubility, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Multi-walled carbon nanotubes have been covalently functionalized via 1,3-dipolar cycloaddition of azomethine ylides with orthogonally protected amino functions that can be selectively deprotected and subsequently modified with drugs and fluorescent probes.
Brough Peter, Klumpp Cedric, Bianco Alberto, Campidelli Stephane, Prato Maurizio
[60]fullerene-pyrrolidine-N-oxides Journal Article
In: The Journal of Organic Chemistry, vol. 71, no. 5, pp. 2014–2020, 2006, ISSN: 0022-3263.
Abstract | Links | BibTeX | Tags: Chromatography, Fullerenes, High Pressure Liquid, I2CT, Nitrogen, Oxidation-Reduction, Oxides, Pyrrolidines, Spectrum Analysis, Team-Bianco
@article{brough_60fullerene-pyrrolidine-n-oxides_2006,
title = {[60]fullerene-pyrrolidine-N-oxides},
author = {Peter Brough and Cedric Klumpp and Alberto Bianco and Stephane Campidelli and Maurizio Prato},
doi = {10.1021/jo052388s},
issn = {0022-3263},
year = {2006},
date = {2006-03-01},
journal = {The Journal of Organic Chemistry},
volume = {71},
number = {5},
pages = {2014--2020},
abstract = {Eight members of a new family of fullerene derivatives, [60]fulleropyrrolidine-N-oxides, have been synthesized and characterized. Facile oxidation, by a peracid, of the parent [60]fulleropyrrolidine gave clean conversions into the product molecules, in which the tertiary amine is transformed into a quaternary amine bearing an oxygen atom. The reaction is very selective, favoring the nitrogen atom of the pyrrolidine ring in preference to epoxidation of the fullerene cage. The 1H NMR shows an AB quartet splitting pattern, characteristic of nonequivalent hydrogens in the pyrrolidine ring and at a chemical shift displacement of 0.8 ppm downfield. Other methods of characterization are described, including MS, differential scanning calorimetry, thermogravimetric analysis, HPLC, UV/vis, and IR. Conclusive evidence for the formation of an N-oxide moiety is provided by the synthesis, oxidation, and NMR characterization of a novel [60]fulleropyrrolidine containing a 15N isotope, showing an 85 ppm downfield heteroatom chemical shift. Preliminary details of the effects of substitution on the reactivity of the pyrrolidine ring are also reported.},
keywords = {Chromatography, Fullerenes, High Pressure Liquid, I2CT, Nitrogen, Oxidation-Reduction, Oxides, Pyrrolidines, Spectrum Analysis, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Eight members of a new family of fullerene derivatives, [60]fulleropyrrolidine-N-oxides, have been synthesized and characterized. Facile oxidation, by a peracid, of the parent [60]fulleropyrrolidine gave clean conversions into the product molecules, in which the tertiary amine is transformed into a quaternary amine bearing an oxygen atom. The reaction is very selective, favoring the nitrogen atom of the pyrrolidine ring in preference to epoxidation of the fullerene cage. The 1H NMR shows an AB quartet splitting pattern, characteristic of nonequivalent hydrogens in the pyrrolidine ring and at a chemical shift displacement of 0.8 ppm downfield. Other methods of characterization are described, including MS, differential scanning calorimetry, thermogravimetric analysis, HPLC, UV/vis, and IR. Conclusive evidence for the formation of an N-oxide moiety is provided by the synthesis, oxidation, and NMR characterization of a novel [60]fulleropyrrolidine containing a 15N isotope, showing an 85 ppm downfield heteroatom chemical shift. Preliminary details of the effects of substitution on the reactivity of the pyrrolidine ring are also reported.
Klumpp Cédric, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Functionalized carbon nanotubes as emerging nanovectors for the delivery of therapeutics Journal Article
In: Biochimica Et Biophysica Acta, vol. 1758, no. 3, pp. 404–412, 2006, ISSN: 0006-3002.
Abstract | Links | BibTeX | Tags: carbon, DNA, Drug Carriers, Drug Delivery Systems, Humans, I2CT, Nanotubes, RNA, Team-Bianco
@article{klumpp_functionalized_2006,
title = {Functionalized carbon nanotubes as emerging nanovectors for the delivery of therapeutics},
author = {Cédric Klumpp and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1016/j.bbamem.2005.10.008},
issn = {0006-3002},
year = {2006},
date = {2006-03-01},
journal = {Biochimica Et Biophysica Acta},
volume = {1758},
number = {3},
pages = {404--412},
abstract = {Functionalized carbon nanotubes (f-CNT) are emerging as a new family of nanovectors for the delivery of different types of therapeutic molecules. The application of CNT in the field of carrier-mediated delivery has become possible after the recent discovery of their capacity to penetrate into the cells. CNT can be loaded with active molecules by forming stable covalent bonds or supramolecular assemblies based on noncovalent interactions. Once the cargos are carried into various cells, tissues and organs they are able to express their biological function. In this review, we will describe the potential of f-CNT to deliver different types of therapeutic molecules.},
keywords = {carbon, DNA, Drug Carriers, Drug Delivery Systems, Humans, I2CT, Nanotubes, RNA, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalized carbon nanotubes (f-CNT) are emerging as a new family of nanovectors for the delivery of different types of therapeutic molecules. The application of CNT in the field of carrier-mediated delivery has become possible after the recent discovery of their capacity to penetrate into the cells. CNT can be loaded with active molecules by forming stable covalent bonds or supramolecular assemblies based on noncovalent interactions. Once the cargos are carried into various cells, tissues and organs they are able to express their biological function. In this review, we will describe the potential of f-CNT to deliver different types of therapeutic molecules.
Singh Ravi, Pantarotto Davide, Lacerda Lara, Pastorin Giorgia, Klumpp Cédric, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers Journal Article
In: Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 9, pp. 3357–3362, 2006, ISSN: 0027-8424.
Abstract | Links | BibTeX | Tags: Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission
@article{singh_tissue_2006,
title = {Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers},
author = {Ravi Singh and Davide Pantarotto and Lara Lacerda and Giorgia Pastorin and Cédric Klumpp and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1073/pnas.0509009103},
issn = {0027-8424},
year = {2006},
date = {2006-02-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {103},
number = {9},
pages = {3357--3362},
abstract = {Carbon nanotubes (CNT) are intensively being developed for biomedical applications including drug and gene delivery. Although all possible clinical applications will require compatibility of CNT with the biological milieu, their in vivo capabilities and limitations have not yet been explored. In this work, water-soluble, single-walled CNT (SWNT) have been functionalized with the chelating molecule diethylentriaminepentaacetic (DTPA) and labeled with indium ((111)In) for imaging purposes. Intravenous (i.v.) administration of these functionalized SWNT (f-SWNT) followed by radioactivity tracing using gamma scintigraphy indicated that f-SWNT are not retained in any of the reticuloendothelial system organs (liver or spleen) and are rapidly cleared from systemic blood circulation through the renal excretion route. The observed rapid blood clearance and half-life (3 h) of f-SWNT has major implications for all potential clinical uses of CNT. Moreover, urine excretion studies using both f-SWNT and functionalized multiwalled CNT followed by electron microscopy analysis of urine samples revealed that both types of nanotubes were excreted as intact nanotubes. This work describes the pharmacokinetic parameters of i.v. administered functionalized CNT relevant for various therapeutic and diagnostic applications.},
keywords = {Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNT) are intensively being developed for biomedical applications including drug and gene delivery. Although all possible clinical applications will require compatibility of CNT with the biological milieu, their in vivo capabilities and limitations have not yet been explored. In this work, water-soluble, single-walled CNT (SWNT) have been functionalized with the chelating molecule diethylentriaminepentaacetic (DTPA) and labeled with indium ((111)In) for imaging purposes. Intravenous (i.v.) administration of these functionalized SWNT (f-SWNT) followed by radioactivity tracing using gamma scintigraphy indicated that f-SWNT are not retained in any of the reticuloendothelial system organs (liver or spleen) and are rapidly cleared from systemic blood circulation through the renal excretion route. The observed rapid blood clearance and half-life (3 h) of f-SWNT has major implications for all potential clinical uses of CNT. Moreover, urine excretion studies using both f-SWNT and functionalized multiwalled CNT followed by electron microscopy analysis of urine samples revealed that both types of nanotubes were excreted as intact nanotubes. This work describes the pharmacokinetic parameters of i.v. administered functionalized CNT relevant for various therapeutic and diagnostic applications.
Campidelli Stéphane, Klumpp Cédric, Bianco Alberto, Guldi Dirk M, Prato Maurizio
Functionalization of CNT: synthesis and applications in photovoltaics and biology Journal Article
In: Journal of Physical Organic Chemistry, vol. 19, no. 8-9, pp. 531–539, 2006, ISSN: 1099-1395.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, Cells, Drug delivery, electron transfer, Functionalization, I2CT, Peptides, photovoltaic, Team-Bianco, Toxicity, Vectors
@article{campidelli_functionalization_2006,
title = {Functionalization of CNT: synthesis and applications in photovoltaics and biology},
author = {Stéphane Campidelli and Cédric Klumpp and Alberto Bianco and Dirk M Guldi and Maurizio Prato},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/poc.1052},
doi = {10.1002/poc.1052},
issn = {1099-1395},
year = {2006},
date = {2006-01-01},
urldate = {2020-03-31},
journal = {Journal of Physical Organic Chemistry},
volume = {19},
number = {8-9},
pages = {531--539},
abstract = {Here, we review part of the work carried out in our laboratories on carbon nanotube functionalization. Both covalent (sidewall derivatization) and non-covalent (using π-π interactions) functionalization have been used to solubilize carbon nanotubes (NTs). The combination of NTs with various electron donors, mainly using the supramolecular approach, led to a new generation of donor-acceptor nanohybrids which can be used for the development of carbon-based photovoltaic cells. Covalent functionalization has been successfully applied for preparation of water soluble nanotubes and further derivatization of the nanotubes with bioactive molecules hold great promise for application in drug, vaccine and gene delivery. Copyright © 2006 John Wiley & Sons, Ltd.},
keywords = {Carbon nanotubes, Cells, Drug delivery, electron transfer, Functionalization, I2CT, Peptides, photovoltaic, Team-Bianco, Toxicity, Vectors},
pubstate = {published},
tppubtype = {article}
}
Here, we review part of the work carried out in our laboratories on carbon nanotube functionalization. Both covalent (sidewall derivatization) and non-covalent (using π-π interactions) functionalization have been used to solubilize carbon nanotubes (NTs). The combination of NTs with various electron donors, mainly using the supramolecular approach, led to a new generation of donor-acceptor nanohybrids which can be used for the development of carbon-based photovoltaic cells. Covalent functionalization has been successfully applied for preparation of water soluble nanotubes and further derivatization of the nanotubes with bioactive molecules hold great promise for application in drug, vaccine and gene delivery. Copyright © 2006 John Wiley & Sons, Ltd.
Lacerda L, Pastorin G, Wu W, Prato M, Bianco A, Kostarelos K
In: Advanced Functional Materials, vol. 16, no. 14, pp. 1839–1846, 2006, ISSN: 1616-3028.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, DNA, I2CT, Luminescence, multiwalled, single-walled, Team-Bianco
@article{lacerda_luminescence_2006,
title = {Luminescence of Functionalized Carbon Nanotubes as a Tool to Monitor Bundle Formation and Dissociation in Water: The Effect of Plasmid-DNA Complexation},
author = {L Lacerda and G Pastorin and W Wu and M Prato and A Bianco and K Kostarelos},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.200500569},
doi = {10.1002/adfm.200500569},
issn = {1616-3028},
year = {2006},
date = {2006-01-01},
urldate = {2020-03-31},
journal = {Advanced Functional Materials},
volume = {16},
number = {14},
pages = {1839--1846},
abstract = {Functionalized carbon nanotubes (f-CNTs) are explored as novel nanomaterials for biomedical applications. UV-vis luminescence of aqueous dispersions of CNT–NH3+ and CNT–NH–Ac (NH–Ac: acetamido) is observed using standard laboratory spectrophotometric instrumentation, and the measured fluorescence intensity is correlated with the aggregation state of the f-CNTs: a high intensity indicates improved f-CNT individualization and dispersion, while a decrease in fluorescence intensity indicates a higher degree of nanotube aggregation and bundling as a result of varying the sodium dodecyl sulfate (SDS) concentrations and pH in the aqueous phase. Moreover, utilization of this relationship between fluorescence intensity and the state of f-CNT aggregation is carried out to elucidate the interactions between f-CNTs and gene-encoding plasmid DNA (pDNA). pDNA is shown to interact with CNT–NH3+ primarily through electrostatic interactions that lead concomitantly to a higher degree of f-CNT bundling. The CNT–NH3+/pDNA interactions are successfully competed by SDS/f-CNT surface interactions, resulting in the displacement of pDNA. These studies provide exemplification of the use of fluorescence spectrophotometry to accurately describe the aggregation state of water-soluble f-CNTs. Characterization of the complexes between pDNA and f-CNTs elucidates the opportunities and limitations of such supramolecular systems as potential vectors for gene transfer.},
keywords = {Carbon nanotubes, DNA, I2CT, Luminescence, multiwalled, single-walled, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalized carbon nanotubes (f-CNTs) are explored as novel nanomaterials for biomedical applications. UV-vis luminescence of aqueous dispersions of CNT–NH3+ and CNT–NH–Ac (NH–Ac: acetamido) is observed using standard laboratory spectrophotometric instrumentation, and the measured fluorescence intensity is correlated with the aggregation state of the f-CNTs: a high intensity indicates improved f-CNT individualization and dispersion, while a decrease in fluorescence intensity indicates a higher degree of nanotube aggregation and bundling as a result of varying the sodium dodecyl sulfate (SDS) concentrations and pH in the aqueous phase. Moreover, utilization of this relationship between fluorescence intensity and the state of f-CNT aggregation is carried out to elucidate the interactions between f-CNTs and gene-encoding plasmid DNA (pDNA). pDNA is shown to interact with CNT–NH3+ primarily through electrostatic interactions that lead concomitantly to a higher degree of f-CNT bundling. The CNT–NH3+/pDNA interactions are successfully competed by SDS/f-CNT surface interactions, resulting in the displacement of pDNA. These studies provide exemplification of the use of fluorescence spectrophotometry to accurately describe the aggregation state of water-soluble f-CNTs. Characterization of the complexes between pDNA and f-CNTs elucidates the opportunities and limitations of such supramolecular systems as potential vectors for gene transfer.
2005
Fournel Sylvie, Wieckowski Sébastien, Sun Weimin, Trouche Nathalie, Dumortier Hélène, Bianco Alberto, Chaloin Olivier, Habib Mohammed, Peter Jean-Christophe, Schneider Pascal, Vray Bernard, Toes René E, Offringa Rienk, Melief Cornelis J M, Hoebeke Johan, Guichard Gilles
C3-symmetric peptide scaffolds are functional mimetics of trimeric CD40L Journal Article
In: Nature Chemical Biology, vol. 1, no. 7, pp. 377–382, 2005, ISSN: 1552-4450.
Abstract | Links | BibTeX | Tags: Animals, Apoptosis, Biological, CD40 Antigens, CD40 Ligand, Cell Line, Dumortier, Humans, I2CT, Inbred BALB C, Mice, Models, Molecular Mimicry, Molecular Structure, Peptides, Protein Conformation, Protein Structure, Quaternary, Structure-Activity Relationship, Team-Bianco, Team-Dumortier, Time Factors, tumor
@article{fournel_c3-symmetric_2005,
title = {C3-symmetric peptide scaffolds are functional mimetics of trimeric CD40L},
author = {Sylvie Fournel and Sébastien Wieckowski and Weimin Sun and Nathalie Trouche and Hélène Dumortier and Alberto Bianco and Olivier Chaloin and Mohammed Habib and Jean-Christophe Peter and Pascal Schneider and Bernard Vray and René E Toes and Rienk Offringa and Cornelis J M Melief and Johan Hoebeke and Gilles Guichard},
doi = {10.1038/nchembio746},
issn = {1552-4450},
year = {2005},
date = {2005-12-01},
journal = {Nature Chemical Biology},
volume = {1},
number = {7},
pages = {377--382},
abstract = {Interaction between CD40, a member of the tumor necrosis factor receptor (TNFR) superfamily, and its ligand CD40L, a 39-kDa glycoprotein, is essential for the development of humoral and cellular immune responses. Selective blockade or activation of this pathway provides the ground for the development of new treatments against immunologically based diseases and malignancies. Like other members of the TNF superfamily, CD40L monomers self-assemble around a threefold symmetry axis to form noncovalent homotrimers that can each bind three receptor molecules. Here, we report on the structure-based design of small synthetic molecules with C3 symmetry that can mimic CD40L homotrimers. These molecules interact with CD40, compete with the binding of CD40L to CD40, and reproduce, to a certain extent, the functional properties of the much larger homotrimeric soluble CD40L. Architectures based on rigid C3-symmetric cores may thus represent a general approach to mimicking homotrimers of the TNF superfamily.},
keywords = {Animals, Apoptosis, Biological, CD40 Antigens, CD40 Ligand, Cell Line, Dumortier, Humans, I2CT, Inbred BALB C, Mice, Models, Molecular Mimicry, Molecular Structure, Peptides, Protein Conformation, Protein Structure, Quaternary, Structure-Activity Relationship, Team-Bianco, Team-Dumortier, Time Factors, tumor},
pubstate = {published},
tppubtype = {article}
}
Interaction between CD40, a member of the tumor necrosis factor receptor (TNFR) superfamily, and its ligand CD40L, a 39-kDa glycoprotein, is essential for the development of humoral and cellular immune responses. Selective blockade or activation of this pathway provides the ground for the development of new treatments against immunologically based diseases and malignancies. Like other members of the TNF superfamily, CD40L monomers self-assemble around a threefold symmetry axis to form noncovalent homotrimers that can each bind three receptor molecules. Here, we report on the structure-based design of small synthetic molecules with C3 symmetry that can mimic CD40L homotrimers. These molecules interact with CD40, compete with the binding of CD40L to CD40, and reproduce, to a certain extent, the functional properties of the much larger homotrimeric soluble CD40L. Architectures based on rigid C3-symmetric cores may thus represent a general approach to mimicking homotrimers of the TNF superfamily.
Hayer Silvia, Tohidast-Akrad Makiyeh, Haralambous Silva, Jahn-Schmid Beatrice, Skriner Karl, Trembleau Sylvie, Dumortier Hélène, Pinol-Roma Serafin, Redlich Kurt, Schett Georg, Muller Sylviane, Kollias George, Smolen Josef, Steiner Günter
In: Journal of Immunology (Baltimore, Md.: 1950), vol. 175, no. 12, pp. 8327–8336, 2005, ISSN: 0022-1767.
Abstract | Links | BibTeX | Tags: Animals, Antibody Formation, arthritis, Autoantibodies, Autoantigens, Dumortier, Gene Expression Regulation, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, I2CT, Joints, Mice, rheumatoid, Team-Dumortier, Tissue Distribution, transgenic, Tumor Necrosis Factor-alpha
@article{hayer_aberrant_2005,
title = {Aberrant expression of the autoantigen heterogeneous nuclear ribonucleoprotein-A2 (RA33) and spontaneous formation of rheumatoid arthritis-associated anti-RA33 autoantibodies in TNF-alpha transgenic mice},
author = {Silvia Hayer and Makiyeh Tohidast-Akrad and Silva Haralambous and Beatrice Jahn-Schmid and Karl Skriner and Sylvie Trembleau and Hélène Dumortier and Serafin Pinol-Roma and Kurt Redlich and Georg Schett and Sylviane Muller and George Kollias and Josef Smolen and Günter Steiner},
doi = {10.4049/jimmunol.175.12.8327},
issn = {0022-1767},
year = {2005},
date = {2005-12-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {175},
number = {12},
pages = {8327--8336},
abstract = {Human TNF-alpha transgenic (hTNFtg) mice develop erosive arthritis closely resembling rheumatoid arthritis (RA). To investigate mechanisms leading to pathological autoimmune reactions in RA, we examined hTNFtg animals for the presence of RA-associated autoantibodies including Abs to citrullinated epitopes (anti-cyclic citrullinated peptide), heterogeneous nuclear ribonucleoprotein (hnRNP)-A2 (anti-RA33), and heat shock proteins (hsp) (anti-hsp). Although IgM anti-hsp Abs were detected in 40% of hTNFtg and control mice, IgG anti-hsp Abs were rarely seen, and anti-cyclic citrullinated peptide Abs were not seen at all. In contrast, textgreater50% of hTNFtg mice showed IgG anti-RA33 autoantibodies, which became detectable shortly after the onset of arthritis. These Abs were predominantly directed to a short epitope, which was identical with an epitope previously described in MRL/lpr mice. Incidence of anti-RA33 was significantly decreased in mice treated with the osteoclast inhibitor osteoprotegerin and also in c-fos-deficient mice lacking osteoclasts. Pronounced expression of hnRNP-A2 and a smaller splice variant was seen in joints of hTNFtg mice, whereas expression was low in control animals. Although the closely related hnRNP-A1 was also overexpressed, autoantibodies to this protein were infrequently detected. Because expression of hnRNP-A2 in thymus, spleen, brain, and lung was similar in hTNFtg and control mice, aberrant expression appeared to be restricted to the inflamed joint. Finally, immunization of hTNFtg mice with recombinant hnRNP-A2 or a peptide harboring the major B cell epitope aggravated arthritis. These findings suggest that overproduction of TNF-alpha leads to aberrant expression of hnRNP-A2 in the rheumatoid joint and subsequently to autoimmune reactions, which may enhance the inflammatory and destructive process.},
keywords = {Animals, Antibody Formation, arthritis, Autoantibodies, Autoantigens, Dumortier, Gene Expression Regulation, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, I2CT, Joints, Mice, rheumatoid, Team-Dumortier, Tissue Distribution, transgenic, Tumor Necrosis Factor-alpha},
pubstate = {published},
tppubtype = {article}
}
Human TNF-alpha transgenic (hTNFtg) mice develop erosive arthritis closely resembling rheumatoid arthritis (RA). To investigate mechanisms leading to pathological autoimmune reactions in RA, we examined hTNFtg animals for the presence of RA-associated autoantibodies including Abs to citrullinated epitopes (anti-cyclic citrullinated peptide), heterogeneous nuclear ribonucleoprotein (hnRNP)-A2 (anti-RA33), and heat shock proteins (hsp) (anti-hsp). Although IgM anti-hsp Abs were detected in 40% of hTNFtg and control mice, IgG anti-hsp Abs were rarely seen, and anti-cyclic citrullinated peptide Abs were not seen at all. In contrast, textgreater50% of hTNFtg mice showed IgG anti-RA33 autoantibodies, which became detectable shortly after the onset of arthritis. These Abs were predominantly directed to a short epitope, which was identical with an epitope previously described in MRL/lpr mice. Incidence of anti-RA33 was significantly decreased in mice treated with the osteoclast inhibitor osteoprotegerin and also in c-fos-deficient mice lacking osteoclasts. Pronounced expression of hnRNP-A2 and a smaller splice variant was seen in joints of hTNFtg mice, whereas expression was low in control animals. Although the closely related hnRNP-A1 was also overexpressed, autoantibodies to this protein were infrequently detected. Because expression of hnRNP-A2 in thymus, spleen, brain, and lung was similar in hTNFtg and control mice, aberrant expression appeared to be restricted to the inflamed joint. Finally, immunization of hTNFtg mice with recombinant hnRNP-A2 or a peptide harboring the major B cell epitope aggravated arthritis. These findings suggest that overproduction of TNF-alpha leads to aberrant expression of hnRNP-A2 in the rheumatoid joint and subsequently to autoimmune reactions, which may enhance the inflammatory and destructive process.
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Applications of carbon nanotubes in drug delivery Journal Article
In: Current Opinion in Chemical Biology, vol. 9, no. 6, pp. 674–679, 2005, ISSN: 1367-5931.
Abstract | Links | BibTeX | Tags: Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco
@article{bianco_applications_2005,
title = {Applications of carbon nanotubes in drug delivery},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1016/j.cbpa.2005.10.005},
issn = {1367-5931},
year = {2005},
date = {2005-12-01},
journal = {Current Opinion in Chemical Biology},
volume = {9},
number = {6},
pages = {674--679},
abstract = {The development of new and efficient drug delivery systems is of fundamental importance to improve the pharmacological profiles of many classes of therapeutic molecules. Many different types of drug delivery systems are currently available. Within the family of nanomaterials, carbon nanotubes (CNT) have emerged as a new alternative and efficient tool for transporting and translocating therapeutic molecules. CNT can be functionalised with bioactive peptides, proteins, nucleic acids and drugs, and used to deliver their cargos to cells and organs. Because functionalised CNT display low toxicity and are not immunogenic, such systems hold great potential in the field of nanobiotechnology and nanomedicine.},
keywords = {Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The development of new and efficient drug delivery systems is of fundamental importance to improve the pharmacological profiles of many classes of therapeutic molecules. Many different types of drug delivery systems are currently available. Within the family of nanomaterials, carbon nanotubes (CNT) have emerged as a new alternative and efficient tool for transporting and translocating therapeutic molecules. CNT can be functionalised with bioactive peptides, proteins, nucleic acids and drugs, and used to deliver their cargos to cells and organs. Because functionalised CNT display low toxicity and are not immunogenic, such systems hold great potential in the field of nanobiotechnology and nanomedicine.
Monneaux Fanny, Hoebeke Johan, Sordet Christelle, Nonn Céline, Briand Jean-Paul, Maillère Bernard, Sibilia Jean, Muller Sylviane
Selective modulation of CD4+ Ŧ cells from lupus patients by a promiscuous, protective peptide analog Journal Article
In: Journal of Immunology (Baltimore, Md.: 1950), vol. 175, no. 9, pp. 5839–5847, 2005, ISSN: 0022-1767.
Abstract | Links | BibTeX | Tags: Amino Acid Sequence, CD4-Positive T-Lymphocytes, HLA-DR Antigens, Humans, I2CT, Interleukin-10, Lupus Erythematosus, Lymphocyte Activation, Molecular Sequence Data, Monneaux, Peptide Fragments, Phosphorylation, Ribonucleoprotein, Systemic, Team-Dumortier, U1 Small Nuclear
@article{monneaux_selective_2005,
title = {Selective modulation of CD4+ Ŧ cells from lupus patients by a promiscuous, protective peptide analog},
author = {Fanny Monneaux and Johan Hoebeke and Christelle Sordet and Céline Nonn and Jean-Paul Briand and Bernard Maillère and Jean Sibilia and Sylviane Muller},
doi = {10.4049/jimmunol.175.9.5839},
issn = {0022-1767},
year = {2005},
date = {2005-11-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {175},
number = {9},
pages = {5839--5847},
abstract = {A peptide encompassing residues 131-151 of the spliceosomal U1-70K protein and its analog phosphorylated at Ser140 were synthesized as potential candidates for the treatment of patients with lupus. Studies in the MRL/lpr and (NZB x NZW)F1 lupus models have demonstrated that these sequences contain a CD4+ T cell epitope but administration of the phosphorylated peptide only ameliorates the clinical manifestations of treated MRL/lpr mice. Binding assays with soluble HLA class II molecules and molecular modeling experiments indicate that both peptides behave as promiscuous epitopes and bind to a large panel of human DR molecules. In contrast to normal T cells and T cells from non-lupus autoimmune patients, we found that PBMCs from 40% of lupus patients selected randomly and CFSE-labeled CD4+ T cells proliferate in response to peptide 131-151. Remarkably, however, we observed that phosphorylation of Ser140 prevents CD4+ T cells proliferation but not secretion of regulatory cytokines, suggesting a striking immunomodulatory effect of phosphorylated analog on lupus CD4+ T cells that was unique to patients. The analog might act as an activator of regulatory T cells or as a partial agonist of TCR.},
keywords = {Amino Acid Sequence, CD4-Positive T-Lymphocytes, HLA-DR Antigens, Humans, I2CT, Interleukin-10, Lupus Erythematosus, Lymphocyte Activation, Molecular Sequence Data, Monneaux, Peptide Fragments, Phosphorylation, Ribonucleoprotein, Systemic, Team-Dumortier, U1 Small Nuclear},
pubstate = {published},
tppubtype = {article}
}
A peptide encompassing residues 131-151 of the spliceosomal U1-70K protein and its analog phosphorylated at Ser140 were synthesized as potential candidates for the treatment of patients with lupus. Studies in the MRL/lpr and (NZB x NZW)F1 lupus models have demonstrated that these sequences contain a CD4+ T cell epitope but administration of the phosphorylated peptide only ameliorates the clinical manifestations of treated MRL/lpr mice. Binding assays with soluble HLA class II molecules and molecular modeling experiments indicate that both peptides behave as promiscuous epitopes and bind to a large panel of human DR molecules. In contrast to normal T cells and T cells from non-lupus autoimmune patients, we found that PBMCs from 40% of lupus patients selected randomly and CFSE-labeled CD4+ T cells proliferate in response to peptide 131-151. Remarkably, however, we observed that phosphorylation of Ser140 prevents CD4+ T cells proliferation but not secretion of regulatory cytokines, suggesting a striking immunomodulatory effect of phosphorylated analog on lupus CD4+ T cells that was unique to patients. The analog might act as an activator of regulatory T cells or as a partial agonist of TCR.
Wu Wei, Wieckowski Sébastien, Pastorin Giorgia, Benincasa Monica, Klumpp Cédric, Briand Jean-Paul, Gennaro Renato, Prato Maurizio, Bianco Alberto
Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes Journal Article
In: Angewandte Chemie (International Ed. in English), vol. 44, no. 39, pp. 6358–6362, 2005, ISSN: 1433-7851.
Links | BibTeX | Tags: Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco
@article{wu_targeted_2005,
title = {Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes},
author = {Wei Wu and Sébastien Wieckowski and Giorgia Pastorin and Monica Benincasa and Cédric Klumpp and Jean-Paul Briand and Renato Gennaro and Maurizio Prato and Alberto Bianco},
doi = {10.1002/anie.200501613},
issn = {1433-7851},
year = {2005},
date = {2005-10-01},
journal = {Angewandte Chemie (International Ed. in English)},
volume = {44},
number = {39},
pages = {6358--6362},
keywords = {Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto
Efficient solid-phase synthesis of fullero-peptides using Merrifield strategy Journal Article
In: Chemical Communications (Cambridge, England), no. 25, pp. 3174–3176, 2005, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: Chromatography, Fullerenes, High Pressure Liquid, I2CT, Mass Spectrometry, Peptides, Team-Bianco
@article{bianco_efficient_2005,
title = {Efficient solid-phase synthesis of fullero-peptides using Merrifield strategy},
author = {Alberto Bianco},
doi = {10.1039/b504659a},
issn = {1359-7345},
year = {2005},
date = {2005-07-01},
journal = {Chemical Communications (Cambridge, England)},
number = {25},
pages = {3174--3176},
abstract = {Boc-protected l-fulleropyrrolidino-glutamic acid was readily prepared and employed for the synthesis of fullerene-containing peptides using the solid-phase Boc chemistry developed by Merrifield.},
keywords = {Chromatography, Fullerenes, High Pressure Liquid, I2CT, Mass Spectrometry, Peptides, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Boc-protected l-fulleropyrrolidino-glutamic acid was readily prepared and employed for the synthesis of fullerene-containing peptides using the solid-phase Boc chemistry developed by Merrifield.
Bianco Alberto, Hoebeke Johan, Kostarelos Kostas, Prato Maurizio, Partidos Charalambos D
Carbon nanotubes: on the road to deliver Journal Article
In: Current Drug Delivery, vol. 2, no. 3, pp. 253–259, 2005, ISSN: 1567-2018.
Abstract | Links | BibTeX | Tags: carbon, CpG Islands, DNA, Drug Carriers, I2CT, nanotechnology, Team-Bianco
@article{bianco_carbon_2005,
title = {Carbon nanotubes: on the road to deliver},
author = {Alberto Bianco and Johan Hoebeke and Kostas Kostarelos and Maurizio Prato and Charalambos D Partidos},
doi = {10.2174/1567201054367959},
issn = {1567-2018},
year = {2005},
date = {2005-07-01},
journal = {Current Drug Delivery},
volume = {2},
number = {3},
pages = {253--259},
abstract = {Over the last few years, considerable advances have been made in the field of nanotechnology. The advent of carbon nanotube functionalization has paved the way for their potential application as a delivery system of diverse molecules such as peptides, proteins, plasmid DNA, and synthetic oligodeoxynucleotides. This opens new therapeutic and preventive opportunities to combat diseases. The scope of this review is to summarize our recent work in this rapidly growing field.},
keywords = {carbon, CpG Islands, DNA, Drug Carriers, I2CT, nanotechnology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Over the last few years, considerable advances have been made in the field of nanotechnology. The advent of carbon nanotube functionalization has paved the way for their potential application as a delivery system of diverse molecules such as peptides, proteins, plasmid DNA, and synthetic oligodeoxynucleotides. This opens new therapeutic and preventive opportunities to combat diseases. The scope of this review is to summarize our recent work in this rapidly growing field.
Pastorin Giorgia, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Functionalized Carbon Nanotubes: Towards the Delivery of Therapeutic Molecules Journal Article
In: Journal of Biomedical Nanotechnology, vol. 1, no. 2, pp. 133–142, 2005, ISSN: 15507033, 00000000.
Links | BibTeX | Tags: I2CT, Team-Bianco
@article{pastorin_functionalized_2005,
title = {Functionalized Carbon Nanotubes: Towards the Delivery of Therapeutic Molecules},
author = {Giorgia Pastorin and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
url = {http://www.ingentaselect.com/rpsv/cgi-bin/cgi?ini=xref&body=linker&reqdoi=10.1166/jbn.2005.017},
doi = {10.1166/jbn.2005.017},
issn = {15507033, 00000000},
year = {2005},
date = {2005-06-01},
urldate = {2020-11-26},
journal = {Journal of Biomedical Nanotechnology},
volume = {1},
number = {2},
pages = {133--142},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Fauny Jean Daniel, Silber Joël, Zider Alain
Drosophila Lipid Storage Droplet 2 gene (Lsd-2) is expressed and controls lipid storage in wing imaginal discs Journal Article
In: Developmental Dynamics: An Official Publication of the American Association of Anatomists, vol. 232, no. 3, pp. 725–732, 2005, ISSN: 1058-8388.
Abstract | Links | BibTeX | Tags: Animals, Biological, Drosophila, Drosophila Proteins, Embryo, Fat Body, Genes, I2CT, Imagerie, Insect, Larva, Lipid Metabolism, Metamorphosis, Mutation, Nonmammalian, Nuclear Proteins, Phosphoproteins, Wing
@article{fauny_drosophila_2005,
title = {Drosophila Lipid Storage Droplet 2 gene (Lsd-2) is expressed and controls lipid storage in wing imaginal discs},
author = {Jean Daniel Fauny and Joël Silber and Alain Zider},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15704138},
doi = {10.1002/dvdy.20277},
issn = {1058-8388},
year = {2005},
date = {2005-03-01},
urldate = {2011-10-24},
journal = {Developmental Dynamics: An Official Publication of the American Association of Anatomists},
volume = {232},
number = {3},
pages = {725--732},
abstract = {Lipid droplets are the major neutral lipid storage organelles in higher eukaryotes. The PAT domain proteins (Perilipin, ADRP [adipose differentiation related protein], and TIP47 [tail-interacting 47-kDa protein]) are associated with these structures. Perilipin and ADRP are involved in the regulation of lipid storage and metabolism in mammals. Two genes encoding PAT proteins, Drosophila Lipid Storage Droplet 2 Gene (Lsd-2) and Lsd-2, have been identified in Drosophila. Lsd-2 is expressed in fat bodies and in the female germ line and is involved in lipid storage in these tissues. We showed that Lsd-2 is expressed in third-instar wing imaginal discs in Drosophila, with higher levels in the wing pouch, which corresponds to the presumptive wing region of the wing disc. This specific expression pattern is correlated with a high level of neutral lipid accumulation. We also showed that neutral lipid deposition in the wing disc is severely reduced in an Lsd-2 mutant and is increased with Lsd-2 overexpression. Finally, we showed that overexpression of the vestigial (vg) pro-wing gene induces Lsd-2 expression, suggesting that Lsd-2 mediates a vg role during wing formation. Our results suggest that Lsd-2 function is not restricted to tissues directly involved in lipid storage and could play additional roles during development.},
keywords = {Animals, Biological, Drosophila, Drosophila Proteins, Embryo, Fat Body, Genes, I2CT, Imagerie, Insect, Larva, Lipid Metabolism, Metamorphosis, Mutation, Nonmammalian, Nuclear Proteins, Phosphoproteins, Wing},
pubstate = {published},
tppubtype = {article}
}
Lipid droplets are the major neutral lipid storage organelles in higher eukaryotes. The PAT domain proteins (Perilipin, ADRP [adipose differentiation related protein], and TIP47 [tail-interacting 47-kDa protein]) are associated with these structures. Perilipin and ADRP are involved in the regulation of lipid storage and metabolism in mammals. Two genes encoding PAT proteins, Drosophila Lipid Storage Droplet 2 Gene (Lsd-2) and Lsd-2, have been identified in Drosophila. Lsd-2 is expressed in fat bodies and in the female germ line and is involved in lipid storage in these tissues. We showed that Lsd-2 is expressed in third-instar wing imaginal discs in Drosophila, with higher levels in the wing pouch, which corresponds to the presumptive wing region of the wing disc. This specific expression pattern is correlated with a high level of neutral lipid accumulation. We also showed that neutral lipid deposition in the wing disc is severely reduced in an Lsd-2 mutant and is increased with Lsd-2 overexpression. Finally, we showed that overexpression of the vestigial (vg) pro-wing gene induces Lsd-2 expression, suggesting that Lsd-2 mediates a vg role during wing formation. Our results suggest that Lsd-2 function is not restricted to tissues directly involved in lipid storage and could play additional roles during development.
Singh Ravi, Pantarotto Davide, McCarthy David, Chaloin Olivier, Hoebeke Johan, Partidos Charalambos D, Briand Jean-Paul, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors Journal Article
In: Journal of the American Chemical Society, vol. 127, no. 12, pp. 4388–4396, 2005, ISSN: 0002-7863.
Abstract | Links | BibTeX | Tags: carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco
@article{singh_binding_2005,
title = {Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors},
author = {Ravi Singh and Davide Pantarotto and David McCarthy and Olivier Chaloin and Johan Hoebeke and Charalambos D Partidos and Jean-Paul Briand and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1021/ja0441561},
issn = {0002-7863},
year = {2005},
date = {2005-03-01},
journal = {Journal of the American Chemical Society},
volume = {127},
number = {12},
pages = {4388--4396},
abstract = {Carbon nanotubes (CNTs) constitute a class of nanomaterials that possess characteristics suitable for a variety of possible applications. Their compatibility with aqueous environments has been made possible by the chemical functionalization of their surface, allowing for exploration of their interactions with biological components including mammalian cells. Functionalized CNTs (f-CNTs) are being intensively explored in advanced biotechnological applications ranging from molecular biosensors to cellular growth substrates. We have been exploring the potential of f-CNTs as delivery vehicles of biologically active molecules in view of possible biomedical applications, including vaccination and gene delivery. Recently we reported the capability of ammonium-functionalized single-walled CNTs to penetrate human and murine cells and facilitate the delivery of plasmid DNA leading to expression of marker genes. To optimize f-CNTs as gene delivery vehicles, it is essential to characterize their interactions with DNA. In the present report, we study the interactions of three types of f-CNTs, ammonium-functionalized single-walled and multiwalled carbon nanotubes (SWNT-NH3+; MWNT-NH3+), and lysine-functionalized single-walled carbon nanotubes (SWNT-Lys-NH3+), with plasmid DNA. Nanotube-DNA complexes were analyzed by scanning electron microscopy, surface plasmon resonance, PicoGreen dye exclusion, and agarose gel shift assay. The results indicate that all three types of cationic carbon nanotubes are able to condense DNA to varying degrees, indicating that both nanotube surface area and charge density are critical parameters that determine the interaction and electrostatic complex formation between f-CNTs with DNA. All three different f-CNT types in this study exhibited upregulation of marker gene expression over naked DNA using a mammalian (human) cell line. Differences in the levels of gene expression were correlated with the structural and biophysical data obtained for the f-CNT:DNA complexes to suggest that large surface area leading to very efficient DNA condensation is not necessary for effective gene transfer. However, it will require further investigation to determine whether the degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo. This study constitutes the first thorough investigation into the physicochemical interactions between cationic functionalized carbon nanotubes and DNA toward construction of carbon nanotube-based gene transfer vector systems.},
keywords = {carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) constitute a class of nanomaterials that possess characteristics suitable for a variety of possible applications. Their compatibility with aqueous environments has been made possible by the chemical functionalization of their surface, allowing for exploration of their interactions with biological components including mammalian cells. Functionalized CNTs (f-CNTs) are being intensively explored in advanced biotechnological applications ranging from molecular biosensors to cellular growth substrates. We have been exploring the potential of f-CNTs as delivery vehicles of biologically active molecules in view of possible biomedical applications, including vaccination and gene delivery. Recently we reported the capability of ammonium-functionalized single-walled CNTs to penetrate human and murine cells and facilitate the delivery of plasmid DNA leading to expression of marker genes. To optimize f-CNTs as gene delivery vehicles, it is essential to characterize their interactions with DNA. In the present report, we study the interactions of three types of f-CNTs, ammonium-functionalized single-walled and multiwalled carbon nanotubes (SWNT-NH3+; MWNT-NH3+), and lysine-functionalized single-walled carbon nanotubes (SWNT-Lys-NH3+), with plasmid DNA. Nanotube-DNA complexes were analyzed by scanning electron microscopy, surface plasmon resonance, PicoGreen dye exclusion, and agarose gel shift assay. The results indicate that all three types of cationic carbon nanotubes are able to condense DNA to varying degrees, indicating that both nanotube surface area and charge density are critical parameters that determine the interaction and electrostatic complex formation between f-CNTs with DNA. All three different f-CNT types in this study exhibited upregulation of marker gene expression over naked DNA using a mammalian (human) cell line. Differences in the levels of gene expression were correlated with the structural and biophysical data obtained for the f-CNT:DNA complexes to suggest that large surface area leading to very efficient DNA condensation is not necessary for effective gene transfer. However, it will require further investigation to determine whether the degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo. This study constitutes the first thorough investigation into the physicochemical interactions between cationic functionalized carbon nanotubes and DNA toward construction of carbon nanotube-based gene transfer vector systems.
Dumortier Hélène, van Mierlo Geertje J D, Egan Deirdre, van Ewijk Willem, Toes René E M, Offringa Rienk, Melief Cornelis J M
In: Journal of Immunology (Baltimore, Md.: 1950), vol. 175, no. 2, pp. 855–863, 2005, ISSN: 0022-1767.
Abstract | Links | BibTeX | Tags: Adenovirus E1A Proteins, Animals, Antigen, Antigen Presentation, CD8-Positive T-Lymphocytes, Cell Differentiation, Cell Line, Cell Movement, Clonal Deletion, Cytotoxic, Cytotoxicity, Dendritic Cells, Down-Regulation, Dumortier, Epitopes, Female, I2CT, Immunologic, Immunologic Memory, Inbred C57BL, Lipopolysaccharides, Lymphocyte Activation, Mice, Myeloid Cells, Receptors, Regulatory, T-Cell, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic
@article{dumortier_antigen_2005,
title = {Antigen presentation by an immature myeloid dendritic cell line does not cause CTL deletion in vivo, but generates CD8+ central memory-like Ŧ cells that can be rescued for full effector function},
author = {Hélène Dumortier and Geertje J D van Mierlo and Deirdre Egan and Willem van Ewijk and René E M Toes and Rienk Offringa and Cornelis J M Melief},
doi = {10.4049/jimmunol.175.2.855},
issn = {0022-1767},
year = {2005},
date = {2005-01-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {175},
number = {2},
pages = {855--863},
abstract = {Immature dendritic cells (DC), in contrast to their mature counterparts, are incapable of mobilizing a CD8+ CTL response, and, instead, have been reported to induce CTL tolerance. We directly addressed the impact of immature vs mature DC on CTL responses by infusing adenovirus peptide-loaded DC (of the D1 cell line) into mice that had received adenovirus-specific naive TCR-transgenic CD8+ T cells. Whereas i.v. injection of mature DC triggered vigorous CTL expansion, immature DC elicited little proliferation involving only a minority of the TCR-transgenic CTL. Even though the latter CTL developed effector functions, including cytolytic activity and proinflammatory cytokine secretion, these cells differed significantly from CTL primed by mature DC in that they did not exhibit down-regulation of CD62L and CCR7, receptors involved in trapping of T cells in the lymphoid organs. Interestingly, adoptive transfer of CTL effector cells harvested after priming by either mature or immature DC into naive recipient mice, followed by exposure to adenovirus, yielded quantitatively and qualitatively indistinguishable CTL memory responses. Therefore, in vivo priming of naive CD8+ T cells by immature DC, although failing to induce a full-blown, systemic CTL response, resulted in the formation of central memory-like T cells that were able to expand and produce IFN-gamma upon secondary antigenic stimulation.},
keywords = {Adenovirus E1A Proteins, Animals, Antigen, Antigen Presentation, CD8-Positive T-Lymphocytes, Cell Differentiation, Cell Line, Cell Movement, Clonal Deletion, Cytotoxic, Cytotoxicity, Dendritic Cells, Down-Regulation, Dumortier, Epitopes, Female, I2CT, Immunologic, Immunologic Memory, Inbred C57BL, Lipopolysaccharides, Lymphocyte Activation, Mice, Myeloid Cells, Receptors, Regulatory, T-Cell, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic},
pubstate = {published},
tppubtype = {article}
}
Immature dendritic cells (DC), in contrast to their mature counterparts, are incapable of mobilizing a CD8+ CTL response, and, instead, have been reported to induce CTL tolerance. We directly addressed the impact of immature vs mature DC on CTL responses by infusing adenovirus peptide-loaded DC (of the D1 cell line) into mice that had received adenovirus-specific naive TCR-transgenic CD8+ T cells. Whereas i.v. injection of mature DC triggered vigorous CTL expansion, immature DC elicited little proliferation involving only a minority of the TCR-transgenic CTL. Even though the latter CTL developed effector functions, including cytolytic activity and proinflammatory cytokine secretion, these cells differed significantly from CTL primed by mature DC in that they did not exhibit down-regulation of CD62L and CCR7, receptors involved in trapping of T cells in the lymphoid organs. Interestingly, adoptive transfer of CTL effector cells harvested after priming by either mature or immature DC into naive recipient mice, followed by exposure to adenovirus, yielded quantitatively and qualitatively indistinguishable CTL memory responses. Therefore, in vivo priming of naive CD8+ T cells by immature DC, although failing to induce a full-blown, systemic CTL response, resulted in the formation of central memory-like T cells that were able to expand and produce IFN-gamma upon secondary antigenic stimulation.
Kostarelos K, Lacerda L, Partidos C D, Prato M, Bianco A
Carbon nanotube-mediated delivery of peptides and genes to cells: translating nanobiotechnology to therapeutics Journal Article
In: Journal of Drug Delivery Science and Technology, vol. 15, no. 1, pp. 41–47, 2005, ISSN: 1773-2247.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, gene delivery, gene therapy, I2CT, Nanomedicine, Peptide delivery, Team-Bianco, Vaccination
@article{kostarelos_carbon_2005,
title = {Carbon nanotube-mediated delivery of peptides and genes to cells: translating nanobiotechnology to therapeutics},
author = {K Kostarelos and L Lacerda and C D Partidos and M Prato and A Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S1773224705500054},
doi = {10.1016/S1773-2247(05)50005-4},
issn = {1773-2247},
year = {2005},
date = {2005-01-01},
urldate = {2020-03-31},
journal = {Journal of Drug Delivery Science and Technology},
volume = {15},
number = {1},
pages = {41--47},
abstract = {During the last few years, there has been a tremendous amount of optimism and expectation about nanotechnology and its impact on various fields including medicine and pharmaceutical development. One of the most promising materials being developed during the nanotechnological renaissance we are currently experiencing is the carbon nanotube. Before any biology-related application can even be envisaged, the aqueous solubility of carbon nanotubes has to be resolved. Recently, a variety of methodologies have been proposed which lead to biologically compatible carbon nanotubes. Covalent functionalization of their surface is one methodology, allowing the first attempts towards applications in the field of nanomedicine. The possibility of incorporating functionalized carbon nanotubes into cells and the biological milieu offers numerous advantages for potential applications in biology and pharmacology. One of the most promising is their utilization as a new carrier system for the delivery of therapeutic molecules. In the present article, the first attempts to transform carbon nanotubes from biologically incompatible nanomaterials to biologically relevant components of advanced therapeutics and the ensuing novel structures obtained in our laboratories are presented.},
keywords = {Carbon nanotubes, gene delivery, gene therapy, I2CT, Nanomedicine, Peptide delivery, Team-Bianco, Vaccination},
pubstate = {published},
tppubtype = {article}
}
During the last few years, there has been a tremendous amount of optimism and expectation about nanotechnology and its impact on various fields including medicine and pharmaceutical development. One of the most promising materials being developed during the nanotechnological renaissance we are currently experiencing is the carbon nanotube. Before any biology-related application can even be envisaged, the aqueous solubility of carbon nanotubes has to be resolved. Recently, a variety of methodologies have been proposed which lead to biologically compatible carbon nanotubes. Covalent functionalization of their surface is one methodology, allowing the first attempts towards applications in the field of nanomedicine. The possibility of incorporating functionalized carbon nanotubes into cells and the biological milieu offers numerous advantages for potential applications in biology and pharmacology. One of the most promising is their utilization as a new carrier system for the delivery of therapeutic molecules. In the present article, the first attempts to transform carbon nanotubes from biologically incompatible nanomaterials to biologically relevant components of advanced therapeutics and the ensuing novel structures obtained in our laboratories are presented.
Fournel Sylvie, Wieckowski Sébastien, Sun Weimin, Trouche Nathalie, Dumortier Hélène, Bianco Alberto, Chaloin Olivier, Habib Mohammed, Peter Jean-Christophe, Schneider Pascal, Vray Bernard, Toes René E, Offringa Rienk, Melief Cornelis J M, Hoebeke Johan, Guichard Gilles
C3-symmetric peptide scaffolds are functional mimetics of trimeric CD40L Journal Article
In: Nature Chemical Biology, vol. 1, no. 7, pp. 377–382, 2005, ISSN: 1552-4450.
Abstract | Links | BibTeX | Tags: Animals, Apoptosis, Biological, CD40 Antigens, CD40 Ligand, Cell Line, Humans, I2CT, Inbred BALB C, Mice, Models, Molecular Mimicry, Molecular Structure, Peptides, Protein Conformation, Protein Structure, Quaternary, Structure-Activity Relationship, Team-Bianco, Time Factors, tumor
@article{fournel_c3-symmetric_2005b,
title = {C3-symmetric peptide scaffolds are functional mimetics of trimeric CD40L},
author = {Sylvie Fournel and Sébastien Wieckowski and Weimin Sun and Nathalie Trouche and Hélène Dumortier and Alberto Bianco and Olivier Chaloin and Mohammed Habib and Jean-Christophe Peter and Pascal Schneider and Bernard Vray and René E Toes and Rienk Offringa and Cornelis J M Melief and Johan Hoebeke and Gilles Guichard},
doi = {10.1038/nchembio746},
issn = {1552-4450},
year = {2005},
date = {2005-01-01},
journal = {Nature Chemical Biology},
volume = {1},
number = {7},
pages = {377--382},
abstract = {Interaction between CD40, a member of the tumor necrosis factor receptor (TNFR) superfamily, and its ligand CD40L, a 39-kDa glycoprotein, is essential for the development of humoral and cellular immune responses. Selective blockade or activation of this pathway provides the ground for the development of new treatments against immunologically based diseases and malignancies. Like other members of the TNF superfamily, CD40L monomers self-assemble around a threefold symmetry axis to form noncovalent homotrimers that can each bind three receptor molecules. Here, we report on the structure-based design of small synthetic molecules with C3 symmetry that can mimic CD40L homotrimers. These molecules interact with CD40, compete with the binding of CD40L to CD40, and reproduce, to a certain extent, the functional properties of the much larger homotrimeric soluble CD40L. Architectures based on rigid C3-symmetric cores may thus represent a general approach to mimicking homotrimers of the TNF superfamily.},
keywords = {Animals, Apoptosis, Biological, CD40 Antigens, CD40 Ligand, Cell Line, Humans, I2CT, Inbred BALB C, Mice, Models, Molecular Mimicry, Molecular Structure, Peptides, Protein Conformation, Protein Structure, Quaternary, Structure-Activity Relationship, Team-Bianco, Time Factors, tumor},
pubstate = {published},
tppubtype = {article}
}
Interaction between CD40, a member of the tumor necrosis factor receptor (TNFR) superfamily, and its ligand CD40L, a 39-kDa glycoprotein, is essential for the development of humoral and cellular immune responses. Selective blockade or activation of this pathway provides the ground for the development of new treatments against immunologically based diseases and malignancies. Like other members of the TNF superfamily, CD40L monomers self-assemble around a threefold symmetry axis to form noncovalent homotrimers that can each bind three receptor molecules. Here, we report on the structure-based design of small synthetic molecules with C3 symmetry that can mimic CD40L homotrimers. These molecules interact with CD40, compete with the binding of CD40L to CD40, and reproduce, to a certain extent, the functional properties of the much larger homotrimeric soluble CD40L. Architectures based on rigid C3-symmetric cores may thus represent a general approach to mimicking homotrimers of the TNF superfamily.
Bianco Alberto, Kostarelos Kostas, Partidos Charalambos D, Prato Maurizio
Biomedical applications of functionalised carbon nanotubes Journal Article
In: Chemical Communications (Cambridge, England), no. 5, pp. 571–577, 2005, ISSN: 1359-7345.
Abstract | Links | BibTeX | Tags: Antigens, carbon, Chemical, Drug Delivery Systems, Gene Transfer Techniques, Humans, I2CT, Models, Molecular Structure, nanotechnology, Nanotubes, Team-Bianco, Vaccines
@article{bianco_biomedical_2005,
title = {Biomedical applications of functionalised carbon nanotubes},
author = {Alberto Bianco and Kostas Kostarelos and Charalambos D Partidos and Maurizio Prato},
doi = {10.1039/b410943k},
issn = {1359-7345},
year = {2005},
date = {2005-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {5},
pages = {571--577},
abstract = {The organic functionalisation of carbon nanotubes can improve substantially their solubility and biocompatibility profile; as a consequence, their manipulation and integration into biological systems has become possible so that functionalised carbon nanotubes hold currently strong promise as novel systems for the delivery of drugs, antigens and genes.},
keywords = {Antigens, carbon, Chemical, Drug Delivery Systems, Gene Transfer Techniques, Humans, I2CT, Models, Molecular Structure, nanotechnology, Nanotubes, Team-Bianco, Vaccines},
pubstate = {published},
tppubtype = {article}
}
The organic functionalisation of carbon nanotubes can improve substantially their solubility and biocompatibility profile; as a consequence, their manipulation and integration into biological systems has become possible so that functionalised carbon nanotubes hold currently strong promise as novel systems for the delivery of drugs, antigens and genes.
Bianco Alberto, Hoebeke Johan, Godefroy Sylvie, Chaloin Olivier, Pantarotto Davide, Briand Jean-Paul, Muller Sylviane, Prato Maurizio, Partidos Charalambos D
Cationic carbon nanotubes bind to CpG oligodeoxynucleotides and enhance their immunostimulatory properties Journal Article
In: Journal of the American Chemical Society, vol. 127, no. 1, pp. 58–59, 2005, ISSN: 0002-7863.
Abstract | Links | BibTeX | Tags: Adjuvants, Animals, carbon, Cations, CpG Islands, I2CT, Immunologic, Interferon-gamma, Interleukin-6, Kinetics, Lymphocytes, Mice, Nanotubes, oligonucleotides, Surface Plasmon Resonance, Team-Bianco
@article{bianco_cationic_2005,
title = {Cationic carbon nanotubes bind to CpG oligodeoxynucleotides and enhance their immunostimulatory properties},
author = {Alberto Bianco and Johan Hoebeke and Sylvie Godefroy and Olivier Chaloin and Davide Pantarotto and Jean-Paul Briand and Sylviane Muller and Maurizio Prato and Charalambos D Partidos},
doi = {10.1021/ja044293y},
issn = {0002-7863},
year = {2005},
date = {2005-01-01},
journal = {Journal of the American Chemical Society},
volume = {127},
number = {1},
pages = {58--59},
abstract = {Functionalized cationic carbon nanotubes are able to form a stable complex with CpG ODN based on charge interaction and to increase the immunostimulatory activity of CpG motifs.},
keywords = {Adjuvants, Animals, carbon, Cations, CpG Islands, I2CT, Immunologic, Interferon-gamma, Interleukin-6, Kinetics, Lymphocytes, Mice, Nanotubes, oligonucleotides, Surface Plasmon Resonance, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalized cationic carbon nanotubes are able to form a stable complex with CpG ODN based on charge interaction and to increase the immunostimulatory activity of CpG motifs.
2004
van Mierlo Geertje J D, Boonman Zita F H M, Dumortier Hélène M H, den Boer Annemieke Th, Fransen Marieke F, Nouta Jan, van der Voort Ellen I H, Offringa Rienk, Toes René E M, Melief Cornelis J M
Activation of dendritic cells that cross-present tumor-derived antigen licenses CD8+ CTL to cause tumor eradication Journal Article
In: Journal of Immunology (Baltimore, Md.: 1950), vol. 173, no. 11, pp. 6753–6759, 2004, ISSN: 0022-1767.
Abstract | Links | BibTeX | Tags: Adenovirus E1A Proteins, Animals, Antibodies, Antigen-Presenting Cells, Antigens, CD11c Antigen, CD40 Antigens, Cross-Priming, Cultured, Cytotoxic, Cytotoxicity, Dendritic Cells, Dumortier, Epitopes, Experimental, I2CT, Immunologic, Inbred C57BL, Injections, Intralesional, Intravenous, Knockout, Male, Mice, Monoclonal, Neoplasms, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic, tumor, Tumor Cells, Viral
@article{van_mierlo_activation_2004,
title = {Activation of dendritic cells that cross-present tumor-derived antigen licenses CD8+ CTL to cause tumor eradication},
author = {Geertje J D van Mierlo and Zita F H M Boonman and Hélène M H Dumortier and Annemieke Th den Boer and Marieke F Fransen and Jan Nouta and Ellen I H van der Voort and Rienk Offringa and René E M Toes and Cornelis J M Melief},
doi = {10.4049/jimmunol.173.11.6753},
issn = {0022-1767},
year = {2004},
date = {2004-12-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {173},
number = {11},
pages = {6753--6759},
abstract = {The fate of naive CD8(+) T cells is determined by the environment in which they encounter MHC class I presented peptide Ags. The manner in which tumor Ags are presented is a longstanding matter of debate. Ag presentation might be mediated by tumor cells in tumor draining lymph nodes or via cross-presentation by professional APC. Either pathway is insufficient to elicit protective antitumor immunity. We now demonstrate using a syngeneic mouse tumor model, expressing an Ag derived from the early region 1A of human adenovirus type 5, that the inadequate nature of the antitumor CTL response is not due to direct Ag presentation by the tumor cells, but results from presentation of tumor-derived Ag by nonactivated CD11c(+) APC. Although this event results in division of naive CTL in tumor draining lymph nodes, it does not establish a productive immune response. Treatment of tumor-bearing mice with dendritic cell-stimulating agonistic anti-CD40 mAb resulted in systemic efflux of CTL with robust effector function capable to eradicate established tumors. For efficacy of anti-CD40 treatment, CD40 ligation of host APC is required because adoptive transfer of CD40-proficient tumor-specific TCR transgenic CTL into CD40-deficient tumor-bearing mice did not lead to productive antitumor immunity after CD40 triggering in vivo. CpG and detoxified LPS (MPL) acted similarly as agonistic anti-CD40 mAb with respect to CD8(+) CTL efflux and tumor eradication. Together these results indicate that dendritic cells, depending on their activation state, orchestrate the outcome of CTL-mediated immunity against tumors, leading either to an ineffective immune response or potent antitumor immunity.},
keywords = {Adenovirus E1A Proteins, Animals, Antibodies, Antigen-Presenting Cells, Antigens, CD11c Antigen, CD40 Antigens, Cross-Priming, Cultured, Cytotoxic, Cytotoxicity, Dendritic Cells, Dumortier, Epitopes, Experimental, I2CT, Immunologic, Inbred C57BL, Injections, Intralesional, Intravenous, Knockout, Male, Mice, Monoclonal, Neoplasms, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic, tumor, Tumor Cells, Viral},
pubstate = {published},
tppubtype = {article}
}
The fate of naive CD8(+) T cells is determined by the environment in which they encounter MHC class I presented peptide Ags. The manner in which tumor Ags are presented is a longstanding matter of debate. Ag presentation might be mediated by tumor cells in tumor draining lymph nodes or via cross-presentation by professional APC. Either pathway is insufficient to elicit protective antitumor immunity. We now demonstrate using a syngeneic mouse tumor model, expressing an Ag derived from the early region 1A of human adenovirus type 5, that the inadequate nature of the antitumor CTL response is not due to direct Ag presentation by the tumor cells, but results from presentation of tumor-derived Ag by nonactivated CD11c(+) APC. Although this event results in division of naive CTL in tumor draining lymph nodes, it does not establish a productive immune response. Treatment of tumor-bearing mice with dendritic cell-stimulating agonistic anti-CD40 mAb resulted in systemic efflux of CTL with robust effector function capable to eradicate established tumors. For efficacy of anti-CD40 treatment, CD40 ligation of host APC is required because adoptive transfer of CD40-proficient tumor-specific TCR transgenic CTL into CD40-deficient tumor-bearing mice did not lead to productive antitumor immunity after CD40 triggering in vivo. CpG and detoxified LPS (MPL) acted similarly as agonistic anti-CD40 mAb with respect to CD8(+) CTL efflux and tumor eradication. Together these results indicate that dendritic cells, depending on their activation state, orchestrate the outcome of CTL-mediated immunity against tumors, leading either to an ineffective immune response or potent antitumor immunity.
Bianco Alberto
Carbon nanotubes for the delivery of therapeutic molecules Journal Article
In: Expert Opinion on Drug Delivery, vol. 1, no. 1, pp. 57–65, 2004, ISSN: 1742-5247.
Abstract | Links | BibTeX | Tags: carbon, Drug Carriers, Gene Transfer Techniques, I2CT, Nanotubes, Pharmaceutical Preparations, Team-Bianco
@article{bianco_carbon_2004,
title = {Carbon nanotubes for the delivery of therapeutic molecules},
author = {Alberto Bianco},
doi = {10.1517/17425247.1.1.57},
issn = {1742-5247},
year = {2004},
date = {2004-11-01},
journal = {Expert Opinion on Drug Delivery},
volume = {1},
number = {1},
pages = {57--65},
abstract = {Functionalised carbon nanotubes (f-CNTs) are emerging as new tools in the field of nanobiotechnology and nanomedicine. This is because they can be easily manipulated and modified by encapsulation with biopolymers or by covalent linking of solubilising groups to the external walls and tips. The possibility of incorporating f-CNTs into biological systems has opened the way to the exploration of their potential applications in biology and medicinal chemistry. Within the different fields of applications (i.e., biosensors, composite materials, molecular electronics), one use of CNTs is as new carrier systems for the delivery of therapeutic molecules. Research discussed in this review is focused on recent advances in the development of CNT technology for the delivery of drugs, antigens and genes.},
keywords = {carbon, Drug Carriers, Gene Transfer Techniques, I2CT, Nanotubes, Pharmaceutical Preparations, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Functionalised carbon nanotubes (f-CNTs) are emerging as new tools in the field of nanobiotechnology and nanomedicine. This is because they can be easily manipulated and modified by encapsulation with biopolymers or by covalent linking of solubilising groups to the external walls and tips. The possibility of incorporating f-CNTs into biological systems has opened the way to the exploration of their potential applications in biology and medicinal chemistry. Within the different fields of applications (i.e., biosensors, composite materials, molecular electronics), one use of CNTs is as new carrier systems for the delivery of therapeutic molecules. Research discussed in this review is focused on recent advances in the development of CNT technology for the delivery of drugs, antigens and genes.
Monneaux Fanny, Parietti Véronique, Briand Jean-Paul, Muller Sylviane
Intramolecular Ŧ cell spreading in unprimed MRL/lpr mice: importance of the U1-70k protein sequence 131-151 Journal Article
In: Arthritis and Rheumatism, vol. 50, no. 10, pp. 3232–3238, 2004, ISSN: 0004-3591.
Abstract | Links | BibTeX | Tags: Animals, Cell Division, Female, I2CT, Immunization, Inbred BALB C, Inbred MRL lpr, Lupus Erythematosus, Lymphocytes, Mice, Monneaux, Peptides, Phosphorylation, Ribonucleoprotein, Systemic, Team-Dumortier, U1 Small Nuclear
@article{monneaux_intramolecular_2004,
title = {Intramolecular Ŧ cell spreading in unprimed MRL/lpr mice: importance of the U1-70k protein sequence 131-151},
author = {Fanny Monneaux and Véronique Parietti and Jean-Paul Briand and Sylviane Muller},
doi = {10.1002/art.20510},
issn = {0004-3591},
year = {2004},
date = {2004-10-01},
journal = {Arthritis and Rheumatism},
volume = {50},
number = {10},
pages = {3232--3238},
abstract = {OBJECTIVE: To analyze spontaneous T cell spreading against determinants of the U1-70K protein in young autoimmune MRL/lpr lupus mice, in comparison with the T cell spreading occurring in normal BALB/c mice immunized with peptide 131-151 of this protein.
METHODS: Peripheral blood lymphocytes (PBLs) from both unprimed MRL/lpr mice and immunized BALB/c mice were tested for their ability to proliferate ex vivo in response to 18 overlapping peptides of the U1-70K spliceosomal protein, using assays for lymphocyte proliferation and secretion of interleukin-2.
RESULTS: The proliferative response to peptides of the U1-70K protein evolved rapidly in MRL/lpr mice tested at different ages. At least 5 peptides were recognized by PBLs from 8-week-old autoimmune mice, whereas a different peptide was recognized by PBLs from MRL/lpr mice at 12 weeks of age. At 15 weeks, the proliferative response was weak or negative when assessed with any of the test peptides. At least 2 major peptides recognized by MRL/lpr PBLs were also recognized by PBLs generated in the BALB/c mice primed with peptide 131-151. We further demonstrated that, in preautoimmune MRL/lpr mice, repeated administration of phosphorylated peptide 131-151 (called P140), which was shown previously to be protective, transiently abolished T cell intramolecular spreading to other regions of the 70K protein.
CONCLUSION: This is the first study to demonstrate that intramolecular T cell spreading effectively occurs in MRL/lpr mice with lupus, and that region 131-151 is important in the cascade of events observed in the murine lupus response. This sequence might originate a mechanism of tolerance spreading that leads to the beneficial effect observed in MRL/lpr mice after treatment with the phosphorylated peptide 131-151.},
keywords = {Animals, Cell Division, Female, I2CT, Immunization, Inbred BALB C, Inbred MRL lpr, Lupus Erythematosus, Lymphocytes, Mice, Monneaux, Peptides, Phosphorylation, Ribonucleoprotein, Systemic, Team-Dumortier, U1 Small Nuclear},
pubstate = {published},
tppubtype = {article}
}
OBJECTIVE: To analyze spontaneous T cell spreading against determinants of the U1-70K protein in young autoimmune MRL/lpr lupus mice, in comparison with the T cell spreading occurring in normal BALB/c mice immunized with peptide 131-151 of this protein.
METHODS: Peripheral blood lymphocytes (PBLs) from both unprimed MRL/lpr mice and immunized BALB/c mice were tested for their ability to proliferate ex vivo in response to 18 overlapping peptides of the U1-70K spliceosomal protein, using assays for lymphocyte proliferation and secretion of interleukin-2.
RESULTS: The proliferative response to peptides of the U1-70K protein evolved rapidly in MRL/lpr mice tested at different ages. At least 5 peptides were recognized by PBLs from 8-week-old autoimmune mice, whereas a different peptide was recognized by PBLs from MRL/lpr mice at 12 weeks of age. At 15 weeks, the proliferative response was weak or negative when assessed with any of the test peptides. At least 2 major peptides recognized by MRL/lpr PBLs were also recognized by PBLs generated in the BALB/c mice primed with peptide 131-151. We further demonstrated that, in preautoimmune MRL/lpr mice, repeated administration of phosphorylated peptide 131-151 (called P140), which was shown previously to be protective, transiently abolished T cell intramolecular spreading to other regions of the 70K protein.
CONCLUSION: This is the first study to demonstrate that intramolecular T cell spreading effectively occurs in MRL/lpr mice with lupus, and that region 131-151 is important in the cascade of events observed in the murine lupus response. This sequence might originate a mechanism of tolerance spreading that leads to the beneficial effect observed in MRL/lpr mice after treatment with the phosphorylated peptide 131-151.
METHODS: Peripheral blood lymphocytes (PBLs) from both unprimed MRL/lpr mice and immunized BALB/c mice were tested for their ability to proliferate ex vivo in response to 18 overlapping peptides of the U1-70K spliceosomal protein, using assays for lymphocyte proliferation and secretion of interleukin-2.
RESULTS: The proliferative response to peptides of the U1-70K protein evolved rapidly in MRL/lpr mice tested at different ages. At least 5 peptides were recognized by PBLs from 8-week-old autoimmune mice, whereas a different peptide was recognized by PBLs from MRL/lpr mice at 12 weeks of age. At 15 weeks, the proliferative response was weak or negative when assessed with any of the test peptides. At least 2 major peptides recognized by MRL/lpr PBLs were also recognized by PBLs generated in the BALB/c mice primed with peptide 131-151. We further demonstrated that, in preautoimmune MRL/lpr mice, repeated administration of phosphorylated peptide 131-151 (called P140), which was shown previously to be protective, transiently abolished T cell intramolecular spreading to other regions of the 70K protein.
CONCLUSION: This is the first study to demonstrate that intramolecular T cell spreading effectively occurs in MRL/lpr mice with lupus, and that region 131-151 is important in the cascade of events observed in the murine lupus response. This sequence might originate a mechanism of tolerance spreading that leads to the beneficial effect observed in MRL/lpr mice after treatment with the phosphorylated peptide 131-151.
Poschalko Alexander, Lancelot Nathalie, Marin Julien, Larras Virginie, Limal David, Elbayed Karim, Raya Jesus, Piotto Martial, Briand Jean-Paul, Guichard Gilles, Bianco Alberto
DEUSS: A Perdeuterated Poly(oxyethylene)-Based Resin for Improving HRMAS NMR Studies of Solid-Supported Molecules Journal Article
In: Chemistry – A European Journal, vol. 10, no. 18, pp. 4532–4537, 2004, ISSN: 0947-6539.
Abstract | Links | BibTeX | Tags: combinatorial chemistry, I2CT, NMR spectroscopy, oligoureas, Peptides, poly(oxyethylene), Resins, Team-Bianco
@article{poschalko_deuss_2004,
title = {DEUSS: A Perdeuterated Poly(oxyethylene)-Based Resin for Improving HRMAS NMR Studies of Solid-Supported Molecules},
author = {Alexander Poschalko and Nathalie Lancelot and Julien Marin and Virginie Larras and David Limal and Karim Elbayed and Jesus Raya and Martial Piotto and Jean-Paul Briand and Gilles Guichard and Alberto Bianco},
url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.200400373},
doi = {10.1002/chem.200400373},
issn = {0947-6539},
year = {2004},
date = {2004-09-01},
urldate = {2020-03-31},
journal = {Chemistry – A European Journal},
volume = {10},
number = {18},
pages = {4532--4537},
abstract = {Abstract A novel resin called DEUSS (perdeuterated poly(oxyethylene)-based solid support) has been prepared by anionic polymerization of deuterated [D4]ethylene oxide, followed by cross-linking with deuterated epichlorohydrin. DEUSS can be suspended in a wide range of solvents including organic and aqueous solutions, in which it displays a high swelling capacity. As measured by proton HRMAS of the swollen polymer, the signal intensity of the oxyethylene protons is reduced by a factor of 110 relative to the corresponding nondeuterated poly(oxyethylene)poly(oxypropylene) (POEPOP) resin, thus facilitating detailed HRMAS NMR studies of covalently linked molecules. This 1H NMR invisible matrix was used for the solid-phase synthesis of peptides, oligoureas, and a series of amides as well as their characterization by HRMAS NMR spectroscopy. On-bead NMR spectra of high quality and with resolution comparable to that of liquid samples were obtained and readily interpreted. The complete absence of the parasite resin signals will be of great advantage, for example, for the optimization of multistep solid-phase stereoselective reactions, and for the conformational study of resin-bound molecules in a large variety of solvents.},
keywords = {combinatorial chemistry, I2CT, NMR spectroscopy, oligoureas, Peptides, poly(oxyethylene), Resins, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Abstract A novel resin called DEUSS (perdeuterated poly(oxyethylene)-based solid support) has been prepared by anionic polymerization of deuterated [D4]ethylene oxide, followed by cross-linking with deuterated epichlorohydrin. DEUSS can be suspended in a wide range of solvents including organic and aqueous solutions, in which it displays a high swelling capacity. As measured by proton HRMAS of the swollen polymer, the signal intensity of the oxyethylene protons is reduced by a factor of 110 relative to the corresponding nondeuterated poly(oxyethylene)poly(oxypropylene) (POEPOP) resin, thus facilitating detailed HRMAS NMR studies of covalently linked molecules. This 1H NMR invisible matrix was used for the solid-phase synthesis of peptides, oligoureas, and a series of amides as well as their characterization by HRMAS NMR spectroscopy. On-bead NMR spectra of high quality and with resolution comparable to that of liquid samples were obtained and readily interpreted. The complete absence of the parasite resin signals will be of great advantage, for example, for the optimization of multistep solid-phase stereoselective reactions, and for the conformational study of resin-bound molecules in a large variety of solvents.
