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2021
Husser C, Dentz N, Ryckelynck M
Structure-Switching RNAs: From Gene Expression Regulation to Small Molecule Detection Journal Article
In: Small Structures, vol. 2, no. 4, pp. 2000132, 2021.
Abstract | Links | BibTeX | Tags: biosensing, Gene Expression Regulation, riboswitches, RNA aptamers, RYCKELYNCK, Synthetic Biology, Unité ARN
@article{C2021c,
title = {Structure-Switching RNAs: From Gene Expression Regulation to Small Molecule Detection},
author = {C Husser and N Dentz and M Ryckelynck},
url = {https://doi.org/10.1002/sstr.202000132},
doi = {10.1002/sstr.202000132},
year = {2021},
date = {2021-01-01},
journal = {Small Structures},
volume = {2},
number = {4},
pages = {2000132},
abstract = {RNA is instrumental to cell life in many aspects, especially gene expression regulation. Among the various known regulatory RNAs, riboswitches are particularly interesting cis‐acting molecules as they do not need cellular factor to achieve their function and are therefore highly portable from one organism to the other. These molecules usually found in the 5′ untranslated region of bacterial messenger RNAs are able to specifically sense a target ligand via an aptamer domain prior to transmitting this recognition event to an expression platform that turns on, or off, the expression of downstream genes. In addition to their obvious scientific interest, these modular molecules can also serve for the development of synthetic RNA devices with applications ranging from the control of transgene expression in gene therapy to the specific biosensing of small molecules. The engineering of such nanomachines is greatly facilitated by the proper understanding of their structure as well as the introduction of new technologies. Herein, a general overview of the current knowledge on natural riboswitches prior to explaining the main strategies used to develop new synthetic structure‐switching molecules (riboswitches or biosensors) controlled by small molecules is given.},
keywords = {biosensing, Gene Expression Regulation, riboswitches, RNA aptamers, RYCKELYNCK, Synthetic Biology, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
RNA is instrumental to cell life in many aspects, especially gene expression regulation. Among the various known regulatory RNAs, riboswitches are particularly interesting cis‐acting molecules as they do not need cellular factor to achieve their function and are therefore highly portable from one organism to the other. These molecules usually found in the 5′ untranslated region of bacterial messenger RNAs are able to specifically sense a target ligand via an aptamer domain prior to transmitting this recognition event to an expression platform that turns on, or off, the expression of downstream genes. In addition to their obvious scientific interest, these modular molecules can also serve for the development of synthetic RNA devices with applications ranging from the control of transgene expression in gene therapy to the specific biosensing of small molecules. The engineering of such nanomachines is greatly facilitated by the proper understanding of their structure as well as the introduction of new technologies. Herein, a general overview of the current knowledge on natural riboswitches prior to explaining the main strategies used to develop new synthetic structure‐switching molecules (riboswitches or biosensors) controlled by small molecules is given.
2020
Ryckelynck M
Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More Book Chapter
In: vol. 2166, pp. 73-102, Methods in Molecular Biology, 2020.
Abstract | Links | BibTeX | Tags: aptamer, biosensing, Engineering, fluorogen, Functional screening, Live-cell imaging, RNA, RYCKELYNCK, SELEX, Unité ARN
@inbook{Ryckelynck2020,
title = {Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More},
author = {M Ryckelynck},
url = {https://link.springer.com/protocol/10.1007%2F978-1-0716-0712-1_5},
doi = {10.1007/978-1-0716-0712-1_5 },
year = {2020},
date = {2020-07-25},
journal = {RNA Tagging},
volume = {2166},
pages = {73-102},
publisher = {Methods in Molecular Biology},
abstract = {The central role of RNA in living systems made it highly desirable to have noninvasive and sensitive technologies allowing for imaging the synthesis and the location of these molecules in living cells. This need motivated the development of small pro-fluorescent molecules called “fluorogens” that become fluorescent upon binding to genetically encodable RNAs called “light-up aptamers.” Yet, the development of these fluorogen/light-up RNA pairs is a long and thorough process starting with the careful design of the fluorogen and pursued by the selection of a specific and efficient synthetic aptamer. This chapter summarizes the main design and the selection strategies used up to now prior to introducing the main pairs. Then, the vast application potential of these molecules for live-cell RNA imaging and other applications is presented and discussed.},
keywords = {aptamer, biosensing, Engineering, fluorogen, Functional screening, Live-cell imaging, RNA, RYCKELYNCK, SELEX, Unité ARN},
pubstate = {published},
tppubtype = {inbook}
}
The central role of RNA in living systems made it highly desirable to have noninvasive and sensitive technologies allowing for imaging the synthesis and the location of these molecules in living cells. This need motivated the development of small pro-fluorescent molecules called “fluorogens” that become fluorescent upon binding to genetically encodable RNAs called “light-up aptamers.” Yet, the development of these fluorogen/light-up RNA pairs is a long and thorough process starting with the careful design of the fluorogen and pursued by the selection of a specific and efficient synthetic aptamer. This chapter summarizes the main design and the selection strategies used up to now prior to introducing the main pairs. Then, the vast application potential of these molecules for live-cell RNA imaging and other applications is presented and discussed.
