Publications
2016
Mollerup M Storm, Ross J A, Helfer A C, Meistrup K, Romby P, Kallipolitis B H
In: RNA Biol, vol. 13, no. 9, pp. 895-915, 2016, ISBN: 27400116.
Abstract | Links | BibTeX | Tags: Listeria monocytogenes antisense multiplicity post-transcriptional regulation sRNAs, ROMBY, Unité ARN
@article{,
title = {Two novel members of the LhrC family of small RNAs in Listeria monocytogenes with overlapping regulatory functions but distinctive expression profiles.},
author = {M Storm Mollerup and J A Ross and A C Helfer and K Meistrup and P Romby and B H Kallipolitis},
url = {http://www.ncbi.nlm.nih.gov/pubmed/27400116?dopt=Abstract},
doi = {10.1080/15476286.2016.1208332},
isbn = {27400116},
year = {2016},
date = {2016-01-01},
journal = {RNA Biol},
volume = {13},
number = {9},
pages = {895-915},
abstract = {Multicopy small RNAs (sRNAs) have gained recognition as an important feature of bacterial gene regulation. In the human pathogen Listeria monocytogenes, five homologous sRNAs, called LhrC1-5, control gene expression by base pairing to target mRNAs though three conserved UCCC motifs common to all five LhrCs. We show here that the sRNAs Rli22 and Rli33-1 are structurally and functionally related to LhrC1-5, expanding the LhrC family to seven members, which makes it the largest multicopy sRNA family reported so far. Rli22 and Rli33-1 both contain two UCCC motifs important for post-transcriptional repression of three LhrC target genes. One such target, oppA, encodes a virulence-associated oligo-peptide binding protein. Like LhrC1-5, Rli22 and Rli33-1 employ their UCCC motifs to recognize the Shine-Dalgarno region of oppA mRNA and prevent formation of the ribosomal complex, demonstrating that the seven sRNAs act in a functionally redundant manner. However, differential expression profiles of the sRNAs under infection-relevant conditions suggest that they might also possess non-overlapping functions. Collectively, this makes the LhrC family a unique case for studying the purpose of sRNA multiplicity in the context of bacterial virulence.},
keywords = {Listeria monocytogenes antisense multiplicity post-transcriptional regulation sRNAs, ROMBY, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
Multicopy small RNAs (sRNAs) have gained recognition as an important feature of bacterial gene regulation. In the human pathogen Listeria monocytogenes, five homologous sRNAs, called LhrC1-5, control gene expression by base pairing to target mRNAs though three conserved UCCC motifs common to all five LhrCs. We show here that the sRNAs Rli22 and Rli33-1 are structurally and functionally related to LhrC1-5, expanding the LhrC family to seven members, which makes it the largest multicopy sRNA family reported so far. Rli22 and Rli33-1 both contain two UCCC motifs important for post-transcriptional repression of three LhrC target genes. One such target, oppA, encodes a virulence-associated oligo-peptide binding protein. Like LhrC1-5, Rli22 and Rli33-1 employ their UCCC motifs to recognize the Shine-Dalgarno region of oppA mRNA and prevent formation of the ribosomal complex, demonstrating that the seven sRNAs act in a functionally redundant manner. However, differential expression profiles of the sRNAs under infection-relevant conditions suggest that they might also possess non-overlapping functions. Collectively, this makes the LhrC family a unique case for studying the purpose of sRNA multiplicity in the context of bacterial virulence.