Publications
1996
Yusupova G, Reinbolt J, Wakao H, Laalami S, Grunberg-Manago M, Romby P, Ehresmann B, Ehresmann C
Topography of the Escherichia coli initiation factor 2/fMet-tRNA(f)(Met) complex as studied by cross-linking Journal Article
In: Biochemistry, vol. 35, no. 9, pp. 2978-2984, 1996, ISBN: 8608135, (0006-2960 Journal Article).
Abstract | Links | BibTeX | Tags: Amino Acid Sequence Base Sequence Cisplatin/*pharmacology Cross-Linking Reagents Electrophoresis, Met/chemistry/isolation & purification/*metabolism Substrate Specificity Support, Non-U.S. Gov't, Polyacrylamide Gel Escherichia coli/drug effects/*metabolism Eukaryotic Initiation Factor-2/chemistry/isolation & purification/*metabolism Kinetics Molecular Sequence Data Nucleic Acid Conformation Peptide Fragments/chemistry/isolation & purification Protein Conformation RNA, ROMBY, Transfer, Unité ARN
@article{,
title = {Topography of the Escherichia coli initiation factor 2/fMet-tRNA(f)(Met) complex as studied by cross-linking},
author = {G Yusupova and J Reinbolt and H Wakao and S Laalami and M Grunberg-Manago and P Romby and B Ehresmann and C Ehresmann},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8608135},
isbn = {8608135},
year = {1996},
date = {1996-01-01},
journal = {Biochemistry},
volume = {35},
number = {9},
pages = {2978-2984},
abstract = {trans-Diamminedichloroplatinum(II) was used to induce reversible cross-links between Escherichia coli initiation factor 2 (IF-2) and fMet-tRNA(f)(Met). Two distinct cross-links between IF-2 and the initiator tRNA were produced. Analysis of the cross-linking regions on both RNA and protein moieties reveals that the T arm of the tRNA is in the proximity of a region of the C-terminal domain of IF-2 (residues Asn611-Arg645). This cross-link is well-correlated with the fact that the C-domain of IF-2 contains the fMet-tRNA binding site and that the cross-linked RNA fragment precisely maps in a region which is protected by IF-2 from chemical modification and enzymatic digestion. Rather unexpectedly, a second cross-link was characterized which involves the anticodon arm of fMet-tRNA(f)(Met) and the N-terminal part of IF-2 (residues Trp215-Arg237).},
note = {0006-2960
Journal Article},
keywords = {Amino Acid Sequence Base Sequence Cisplatin/*pharmacology Cross-Linking Reagents Electrophoresis, Met/chemistry/isolation & purification/*metabolism Substrate Specificity Support, Non-U.S. Gov't, Polyacrylamide Gel Escherichia coli/drug effects/*metabolism Eukaryotic Initiation Factor-2/chemistry/isolation & purification/*metabolism Kinetics Molecular Sequence Data Nucleic Acid Conformation Peptide Fragments/chemistry/isolation & purification Protein Conformation RNA, ROMBY, Transfer, Unité ARN},
pubstate = {published},
tppubtype = {article}
}